##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549136_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1880027
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.729801220939912	31.0	31.0	33.0	30.0	34.0
2	32.13766398035773	33.0	31.0	34.0	30.0	34.0
3	32.193609985388505	33.0	31.0	34.0	30.0	34.0
4	35.87891556876577	37.0	35.0	37.0	35.0	37.0
5	35.86369451077032	37.0	35.0	37.0	35.0	37.0
6	35.9045944552924	37.0	35.0	37.0	35.0	37.0
7	36.1033485157394	37.0	35.0	37.0	35.0	37.0
8	36.05442794172637	37.0	35.0	37.0	35.0	37.0
9	37.8578057655555	39.0	38.0	39.0	35.0	39.0
10	37.618452288185225	39.0	37.0	39.0	35.0	39.0
11	37.325536814098946	39.0	37.0	39.0	35.0	39.0
12	36.208532643414166	37.0	35.0	39.0	33.0	39.0
13	35.830497115201005	37.0	35.0	39.0	32.0	39.0
14	36.73729100699086	38.0	35.0	41.0	33.0	41.0
15	37.00634618545372	38.0	35.0	41.0	33.0	41.0
16	37.15354939051407	38.0	35.0	41.0	33.0	41.0
17	37.095747029165004	38.0	35.0	41.0	33.0	41.0
18	37.06412461097633	38.0	35.0	41.0	33.0	41.0
19	36.97615034252168	37.0	35.0	41.0	33.0	41.0
20	36.77806914475165	37.0	35.0	41.0	33.0	41.0
21	36.6172384758304	37.0	35.0	41.0	32.0	41.0
22	36.578055527925926	37.0	35.0	41.0	32.0	41.0
23	36.60643384376927	37.0	35.0	41.0	33.0	41.0
24	36.55606009913687	36.0	35.0	41.0	33.0	41.0
25	36.434334187753684	36.0	35.0	41.0	32.0	41.0
26	36.332334588811754	36.0	35.0	40.0	32.0	41.0
27	36.24814377665853	36.0	35.0	40.0	32.0	41.0
28	36.2694828318955	36.0	35.0	40.0	32.0	41.0
29	36.27206790115248	36.0	35.0	40.0	32.0	41.0
30	36.19313020504493	36.0	35.0	40.0	32.0	41.0
31	35.972513160715245	36.0	35.0	40.0	31.0	41.0
32	35.7646411461112	36.0	35.0	40.0	31.0	41.0
33	35.60834126318399	36.0	35.0	40.0	30.0	41.0
34	35.48265636610538	36.0	35.0	40.0	30.0	41.0
35	35.30429509788955	36.0	35.0	40.0	29.0	41.0
36	35.12729072508001	36.0	35.0	40.0	29.0	41.0
37	35.035207473084164	35.0	34.0	40.0	28.0	41.0
38	34.99798034815458	35.0	34.0	40.0	28.0	41.0
39	34.98207259789354	35.0	34.0	40.0	28.0	41.0
40	34.805838426788554	35.0	34.0	40.0	27.0	41.0
41	34.747067462329	35.0	34.0	40.0	27.0	41.0
42	34.73162619473018	35.0	34.0	40.0	27.0	41.0
43	34.64050675867953	35.0	34.0	40.0	27.0	41.0
44	34.52735838368279	35.0	34.0	40.0	26.0	41.0
45	34.427786409450505	35.0	34.0	40.0	26.0	41.0
46	34.407139365551664	35.0	34.0	40.0	26.0	41.0
47	34.343070072929805	35.0	34.0	40.0	26.0	41.0
48	34.315479511730416	35.0	34.0	40.0	26.0	41.0
49	34.304895089272655	35.0	34.0	40.0	26.0	41.0
50	34.06036296287234	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	11.0
13	6.0
14	11.0
15	44.0
16	91.0
17	282.0
18	645.0
19	1357.0
20	2454.0
21	3938.0
22	6004.0
23	8977.0
24	12739.0
25	18715.0
26	26662.0
27	32740.0
28	34333.0
29	35828.0
30	40170.0
31	48018.0
32	60631.0
33	83020.0
34	175130.0
35	410539.0
36	96506.0
37	116930.0
38	198759.0
39	465481.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.21865749800402	23.01280779478167	28.934212115038772	13.834322592175537
2	31.567312597106316	25.4212306525385	30.18637498291248	12.8250817674427
3	29.73042408433496	23.97582587909642	33.23665032470278	13.057099711865842
4	26.25994201147111	27.098919324030984	32.18570797121531	14.455430693282597
5	23.63599033418137	31.219126108295253	30.891311667332438	14.253571890190939
6	21.004006857348326	40.762446496779035	28.331242051310966	9.902304594561674
7	85.32340227028654	5.375507904939663	7.669996228777566	1.6310935959962278
8	87.32379907309841	3.762658727773591	7.103887337788234	1.8096548613397574
9	83.27928269115284	5.32875325726705	8.819766950155502	2.5721971014246074
10	57.600768499601344	20.00199997127701	13.74347283310293	8.653758696018727
11	52.508182063342716	17.593470732069274	19.660834658225653	10.237512546362366
12	46.93709186091476	20.36125013098216	21.633040376547783	11.068617631555291
13	20.194284443787243	45.24525445645195	22.266861061037954	12.293600038722847
14	13.392307663666534	46.15407119153076	27.494126414141924	12.959494730660785
15	12.408917531503537	21.550701133547552	53.2128527941354	12.82752854081351
16	13.726132656605463	16.776780333473933	51.32383737042075	18.173249639499858
17	14.405112267004677	17.23326313930598	29.386492853560082	38.975131740129264
18	20.413802567729082	21.720432738465988	37.280581608668385	20.585183085136542
19	30.656102279382157	22.271595035603212	26.228293529826963	20.844009155187663
20	33.05548271381209	20.896082875405515	26.018402927192003	20.030031483590395
21	21.268471144297397	29.24000559566432	28.912669871230573	20.578853388807712
22	22.010109429279474	24.9465034278763	25.159691855489307	27.883695287354914
23	18.54739320233167	30.821631816989864	25.721598679167908	24.909376301510562
24	19.010737611747068	21.809314440696863	43.17326293718122	16.006685010374852
25	14.98712518490426	24.167950779430296	40.618565584430435	20.226358451235008
26	14.395059219894183	37.130636953618215	28.30757217848467	20.166731648002926
27	16.303382876948046	36.39506241133771	30.101908110894154	17.199646600820095
28	13.815865410443573	28.321933674356803	41.98157792414683	15.880622991052787
29	14.328943148156913	23.84604050899269	39.85198084921121	21.973035493639188
30	16.51470962917022	32.06453949863486	32.36113098375715	19.059619888437773
31	31.038330832482725	26.186751573248685	25.062565590813325	17.71235200345527
32	31.855340375430778	25.53090992842124	27.06232410491977	15.55142559122821
33	28.498314119956785	27.920556460093394	25.03192773295277	18.549201686997048
34	18.221014910956065	28.183637788180704	28.864053548167128	24.731293752696104
35	19.95886229293515	24.803313994958582	33.11893924927674	22.118884462829524
36	31.610343893997268	24.918471915562915	26.139518209046997	17.33166598139282
37	19.02579058705008	35.074496270532286	29.18367661741028	16.716036525007354
38	19.777641491319006	34.912051795000814	24.27433223033499	21.035974483345186
39	20.504811898978044	32.84527296682441	27.439765492729624	19.210149641467915
40	24.451670108993113	26.605788108362272	24.96623718701912	23.97630459562549
41	16.523752052497116	24.970332872878952	27.85220637788713	30.653708696736803
42	21.671124935971665	25.136926224995705	24.063537385367336	29.128411453665294
43	22.123192911591165	26.4205780023372	24.711134467749666	26.745094618321968
44	18.70627389925783	31.16497794978476	28.739959585686805	21.388788565270605
45	15.844453297745192	40.92749731785767	22.260105838905506	20.967943545491636
46	21.059750737622384	34.88652024678369	25.227882365519218	18.825846650074705
47	21.519318605530664	28.098479436731495	26.62392614574152	23.758275811996317
48	22.631057958210175	24.32736338361098	32.4484701549499	20.593108503228944
49	20.37204784824899	24.74475100623555	33.47537030053292	21.407830844982545
50	19.11531057798638	35.08981519946256	26.936421657774062	18.858452564776996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1634.0
1	3046.5
2	4459.0
3	11886.5
4	19314.0
5	14380.0
6	9446.0
7	9547.5
8	9649.0
9	9835.0
10	10021.0
11	9680.5
12	9340.0
13	8676.5
14	8013.0
15	7419.0
16	6825.0
17	6581.0
18	6337.0
19	6001.0
20	5665.0
21	5433.5
22	5202.0
23	6248.0
24	7294.0
25	10754.0
26	14214.0
27	16720.5
28	19227.0
29	23452.5
30	27678.0
31	29926.0
32	32174.0
33	37776.0
34	43378.0
35	45976.0
36	48574.0
37	50080.5
38	51587.0
39	69684.5
40	87782.0
41	128213.5
42	168645.0
43	197767.0
44	226889.0
45	219299.5
46	211710.0
47	194419.0
48	177128.0
49	165097.0
50	153066.0
51	140954.5
52	128843.0
53	115885.5
54	102928.0
55	92284.5
56	81641.0
57	69928.0
58	58215.0
59	53365.5
60	48516.0
61	42308.5
62	36101.0
63	28888.0
64	21675.0
65	18098.0
66	14521.0
67	11184.0
68	7847.0
69	6835.5
70	5824.0
71	4700.0
72	3576.0
73	2844.0
74	2112.0
75	1840.0
76	1568.0
77	1118.5
78	669.0
79	514.5
80	360.0
81	294.5
82	229.0
83	152.5
84	76.0
85	56.0
86	36.0
87	26.0
88	16.0
89	12.0
90	8.0
91	7.5
92	7.0
93	6.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1880027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.914080158436274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96573814942631	11.126496905396433
2	7.512549022269878	2.090604185800903
3	2.338907793238702	0.9763125155494381
4	1.151453863223602	0.6408568540653726
5	0.7487424408904976	0.5209031170286809
6	0.5103457757988734	0.4260595219790923
7	0.4163422563328258	0.4055113669571413
8	0.31836606036305276	0.35438167068936616
9	0.28379878770185385	0.3553919172855569
>10	3.4367066750718682	11.482137095190387
>50	1.1647181309994707	11.704301986534258
>100	2.1233290992372766	56.20914444748529
>500	0.02397494156851414	2.0433418194543718
>1k	0.0046403112713253175	1.112304959148438
>5k	0.0	0.0
>10k+	3.866926059437765E-4	0.5522516374352813
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10264	0.5459496060428919	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3680	0.1957418696646378	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2371	0.12611521004751527	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2320	0.12340248304944558	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2105	0.11196647707719093	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2038	0.10840269847188365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.040903667872855015	0.0
2	0.0	0.0	0.0	0.1459553506412408	0.0
3	0.0	0.0	0.0	0.20223113816982416	0.0
4	0.0	0.0	0.0	0.30089993388392827	0.0
5	0.0	0.0	0.0	0.5064288970317979	0.0
6	0.0	0.0	0.0	0.7030750090291257	0.0
7	0.0	0.0	0.0	0.8291370283511885	0.0
8	0.0	0.0	0.0	1.1388666226602064	0.0
9	0.0	0.0	0.0	1.2474820840338996	0.0
10	0.0	0.0	0.0	1.4035968632365385	0.0
11	0.0	0.0	0.0	1.5595520702628207	0.0
12	0.0	0.0	0.0	1.7041244620423004	0.0
13	0.0	0.0	0.0	1.7627938322162395	0.0
14	0.0	0.0	0.0	1.7802935808900617	0.0
15	0.0	0.0	0.0	1.8123143976123748	0.0
16	0.0	0.0	0.0	1.8856112172857091	0.0
17	0.0	0.0	0.0	1.9658760219933011	0.0
18	0.0	0.0	0.0	2.1169908730034197	0.0
19	0.0	0.0	0.0	2.1684794952412916	0.0
20	0.0	0.0	0.0	2.228585015002444	0.0
21	0.0	0.0	0.0	2.314700799509794	0.0
22	0.0	0.0	0.0	2.4036888831915713	0.0
23	0.0	0.0	0.0	2.5118256280361932	0.0
24	0.0	0.0	0.0	2.588686226314835	0.0
25	0.0	0.0	0.0	2.6513449008977	0.0
26	0.0	0.0	0.0	2.720120508907585	0.0
27	0.0	0.0	0.0	2.780226028668737	0.0
28	0.0	0.0	0.0	2.842512368173436	0.0
29	0.0	0.0	0.0	2.9117135019869393	0.0
30	0.0	0.0	0.0	3.0128290710718515	0.0
31	0.0	0.0	0.0	3.111551057511408	0.0
32	0.0	0.0	0.0	3.19272010455169	0.0
33	0.0	0.0	0.0	3.270431754437569	0.0
34	0.0	0.0	0.0	3.351866755105113	0.0
35	0.0	0.0	0.0	3.4679289180421344	0.0
36	0.0	0.0	0.0	3.5575552904293395	0.0
37	0.0	0.0	0.0	3.647394425718354	0.0
38	0.0	0.0	0.0	3.7279251840532077	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTACGA	145	0.0	44.000004	10
TGATCAC	25	4.4439228E-5	44.0	35
AACCGTG	35	1.4467332E-7	44.0	38
AACCGTA	20	7.857761E-4	44.0	3
CTATGCG	55	1.8189894E-12	44.0	1
ATAGGCG	20	7.857761E-4	44.0	2
ACGTTAC	20	7.857761E-4	44.0	1
ACCATAC	20	7.857761E-4	44.0	33
GACGTAA	35	1.4467332E-7	44.0	43
CGCATCG	60	0.0	44.0	21
GATCACG	30	2.528579E-6	44.0	1
CGATTCG	50	2.7284841E-11	44.0	10
TCTAGCG	50	2.7284841E-11	44.0	1
CCTCGAG	30	2.528579E-6	44.0	1
ATACTAC	25	4.4439228E-5	44.0	39
CCGTAAA	20	7.857761E-4	44.0	43
TACGCAC	20	7.857761E-4	44.0	20
TGCGCAA	20	7.857761E-4	44.0	12
GTTCGGA	20	7.857761E-4	44.0	42
TATTCGC	25	4.4439228E-5	44.0	38
>>END_MODULE