##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549134_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2831797
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49778038468153	31.0	31.0	33.0	30.0	34.0
2	31.902593300296598	31.0	31.0	34.0	30.0	34.0
3	31.960082943798586	33.0	31.0	34.0	30.0	34.0
4	35.72562475346926	37.0	35.0	37.0	33.0	37.0
5	35.653818052635835	37.0	35.0	37.0	33.0	37.0
6	35.70089699226322	37.0	35.0	37.0	33.0	37.0
7	35.98607915751023	37.0	35.0	37.0	35.0	37.0
8	35.96783667755845	37.0	35.0	37.0	35.0	37.0
9	37.72796496359026	39.0	38.0	39.0	35.0	39.0
10	37.263373045454884	39.0	37.0	39.0	34.0	39.0
11	37.03890815619905	39.0	37.0	39.0	33.0	39.0
12	36.51796580051465	39.0	35.0	39.0	32.0	39.0
13	36.288830731863904	39.0	35.0	39.0	32.0	39.0
14	37.385646287498716	40.0	35.0	41.0	32.0	41.0
15	37.50580073359778	40.0	35.0	41.0	32.0	41.0
16	37.61244608988568	40.0	35.0	41.0	33.0	41.0
17	37.53311166019316	39.0	35.0	41.0	33.0	41.0
18	37.4022491725219	39.0	36.0	41.0	32.0	41.0
19	37.275841100191855	39.0	36.0	41.0	32.0	41.0
20	37.09954421167902	39.0	35.0	41.0	32.0	41.0
21	36.96900660605262	38.0	35.0	41.0	32.0	41.0
22	36.86199081360705	38.0	35.0	41.0	32.0	41.0
23	36.773581580883096	38.0	35.0	41.0	31.0	41.0
24	36.82781604754861	38.0	35.0	41.0	32.0	41.0
25	36.787582937618765	38.0	35.0	41.0	32.0	41.0
26	36.5612090132167	38.0	35.0	41.0	31.0	41.0
27	36.54807671595104	38.0	35.0	41.0	31.0	41.0
28	36.50763490462064	38.0	35.0	41.0	31.0	41.0
29	36.468435767111835	38.0	35.0	41.0	31.0	41.0
30	36.36244970949542	38.0	35.0	41.0	31.0	41.0
31	36.198524117371406	38.0	35.0	41.0	30.0	41.0
32	35.9787509486026	38.0	35.0	41.0	30.0	41.0
33	35.71958618502668	38.0	35.0	41.0	29.0	41.0
34	35.461416549279484	38.0	35.0	41.0	27.0	41.0
35	35.2094454510687	38.0	34.0	41.0	25.0	41.0
36	35.042404522640574	38.0	34.0	40.0	24.0	41.0
37	34.95208095778052	38.0	34.0	40.0	24.0	41.0
38	34.87167300480932	38.0	34.0	40.0	24.0	41.0
39	34.76440542877897	38.0	34.0	40.0	23.0	41.0
40	34.643114248655536	38.0	34.0	40.0	23.0	41.0
41	34.594305312139255	38.0	34.0	40.0	23.0	41.0
42	34.564432054981346	37.0	34.0	40.0	23.0	41.0
43	34.46131025634959	37.0	34.0	40.0	23.0	41.0
44	34.322508287140636	37.0	34.0	40.0	23.0	41.0
45	34.238245184947935	37.0	34.0	40.0	23.0	41.0
46	34.18448532857405	37.0	33.0	40.0	23.0	41.0
47	34.13269807122474	37.0	33.0	40.0	23.0	41.0
48	34.01245922642054	36.0	33.0	40.0	22.0	41.0
49	33.936836574090584	36.0	33.0	40.0	22.0	41.0
50	33.822849942986736	36.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	1.0
13	9.0
14	28.0
15	79.0
16	203.0
17	529.0
18	1119.0
19	2364.0
20	4110.0
21	6724.0
22	10301.0
23	15571.0
24	22316.0
25	34152.0
26	49873.0
27	63017.0
28	66029.0
29	66450.0
30	70977.0
31	81416.0
32	97304.0
33	124251.0
34	209517.0
35	327377.0
36	192868.0
37	248767.0
38	392344.0
39	744097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.087753112246396	23.298492088239374	26.416794706682715	17.19696009283151
2	34.09160331761069	24.847614429989157	28.127934311675588	12.932847940724566
3	28.015991259260463	24.385893480358938	34.580162349207946	13.017952911172658
4	25.27063204036165	27.053563514616336	33.75623323282001	13.919571212202003
5	22.780163973618166	31.900309238268136	31.870540155244182	13.448986632869516
6	20.600841091363538	40.1420017042182	29.198208769908295	10.05894843450996
7	84.79378995033895	3.720287859617056	9.99552580922997	1.4903963808140201
8	85.56556137321991	3.0722541199104314	9.79095606076283	1.571228446106836
9	79.45551888076723	6.806490719497195	11.16284112173295	2.575149278002625
10	36.97916905766904	37.0071018508742	15.83280863705979	10.18092045439698
11	37.219652397399955	21.154588411528085	26.786948358233303	14.838810832838654
12	34.958226172285656	20.966050885709674	28.90104763865489	15.17467530334978
13	21.76038748540238	31.161979478048746	29.95691428446319	17.12071875208569
14	17.28700892048406	33.44989065247262	31.77752501326896	17.485575413774363
15	16.909616049455522	24.014715744101714	42.747802896888444	16.32786530955432
16	19.020643075757196	21.30438728482303	41.07466036583837	18.600309273581402
17	19.034944948384368	21.489252231003846	32.1974350562558	27.27836776435599
18	21.151339591079445	23.78041222587636	34.93883918939105	20.129408993653147
19	25.826180337079247	23.816078624279918	30.322194705340817	20.035546333300022
20	27.486151020006023	23.552288529156574	29.98890810322915	18.97265234760825
21	22.838113042707512	24.720981059023654	32.11197695315025	20.328928945118594
22	22.73143166688855	23.10684699503531	30.627901646904775	23.533819691171367
23	20.650314976673823	26.319541972817966	30.857649753848882	22.17249329665933
24	20.306752214230045	23.67203581330159	36.905964657777375	19.11524731469099
25	18.527422693081462	25.946987019196644	34.057949775354665	21.467640512367236
26	19.14988962838791	30.360580225206824	30.261385261726037	20.22814488467923
27	19.360286065703157	28.60547560436006	32.52281148684034	19.51142684309645
28	17.56277021269533	26.92975520491052	37.042521056417534	18.464953525976615
29	18.878048108674456	24.141455054864455	35.94074716513931	21.03974967132178
30	19.83344851343511	26.82699360158938	33.287025870851615	20.052532014123894
31	25.193119422048966	25.873747306039242	29.271589736128682	19.661543535783107
32	25.68672825064791	26.139868076701827	29.905533482802614	18.26787018984765
33	24.096465954303927	27.19061429897694	29.162189238847276	19.550730507871858
34	19.89115038966423	27.748563897765273	30.753934692352598	21.606351020217904
35	21.411351166767957	26.47650943905937	30.682107509825034	21.430031884347642
36	24.760143470736075	27.173169545698368	28.513943619546172	19.552743364019385
37	20.788177966146584	30.759973260795174	29.4682493130687	18.983599459989538
38	20.39323440204224	30.864429900872132	27.04314610122124	21.699189595864393
39	21.441155563057663	29.15565628468425	28.904190519306294	20.498997632951795
40	22.053134458437523	27.743266907903358	28.150852621144807	22.052746012514316
41	19.223835606860238	27.351324971387424	28.95631289954753	24.468526522204805
42	21.658826533116603	27.342461341685155	27.657844118063547	23.340868007134695
43	22.68785509695787	27.425941901908928	27.52488967252949	22.36131332860371
44	20.576368998201495	29.144214786582513	28.572316447824477	21.70709976739152
45	18.886382039390533	32.59725185103311	26.65664240763021	21.859723701946148
46	20.745519541125297	30.85945073040193	28.013766523518456	20.381263204954312
47	21.59441513639572	27.57973117423318	29.327985021525198	21.497868667845893
48	21.95803583378328	25.665434351403015	30.778336158983148	21.598193655830556
49	20.01418180752363	26.54265118580181	31.109503965150044	22.333663041524517
50	19.443801939192674	29.616070643481862	29.790977248722278	21.149150168603185
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	973.0
1	3210.5
2	5448.0
3	31733.0
4	58018.0
5	41537.5
6	25057.0
7	24014.0
8	22971.0
9	22805.5
10	22640.0
11	21709.0
12	20778.0
13	19377.0
14	17976.0
15	16581.0
16	15186.0
17	14003.0
18	12820.0
19	12900.0
20	12980.0
21	13256.0
22	13532.0
23	14439.0
24	15346.0
25	19484.0
26	23622.0
27	31615.5
28	39609.0
29	44645.5
30	49682.0
31	55784.5
32	61887.0
33	68724.0
34	75561.0
35	87343.0
36	99125.0
37	101187.5
38	103250.0
39	119421.0
40	135592.0
41	167646.0
42	199700.0
43	217531.5
44	235363.0
45	237864.0
46	240365.0
47	226842.0
48	213319.0
49	203325.5
50	193332.0
51	181582.0
52	169832.0
53	156732.0
54	143632.0
55	135074.5
56	126517.0
57	121250.5
58	115984.0
59	108248.5
60	100513.0
61	91799.0
62	83085.0
63	73747.5
64	64410.0
65	53245.5
66	42081.0
67	35119.5
68	28158.0
69	23133.0
70	18108.0
71	14787.5
72	11467.0
73	9054.5
74	6642.0
75	5279.5
76	3917.0
77	2750.0
78	1583.0
79	1229.0
80	875.0
81	614.5
82	354.0
83	280.5
84	207.0
85	209.5
86	212.0
87	127.5
88	43.0
89	36.0
90	29.0
91	18.5
92	8.0
93	5.5
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2831797.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.01970762118772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.11142011205563	13.634729474253241
2	7.661455358400179	2.6079146030550606
3	2.4711467266436764	1.261745843295914
4	1.1975498318510844	0.8152779199963189
5	0.7342053237075504	0.6247979971710995
6	0.4993012299114382	0.5098776568795264
7	0.41602217447857387	0.49564030414892485
8	0.31436768316185765	0.4280356842371995
9	0.2550268930274956	0.3906434839381103
>10	3.332473067719864	13.911657061802652
>50	1.4219040807286687	17.47760295572238
>100	1.5767127742271796	45.2667940031888
>500	0.006311058065062728	0.7232074404002067
>1k	0.0018933174195188184	0.7112130598598795
>5k	0.0	0.0
>10k+	2.103686021687576E-4	1.1408625120507048
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31864	1.125221899733632	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4457	0.15739122543035394	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3136	0.11074240137975992	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02733246768747901	0.0
2	0.0	0.0	0.0	0.10477445946866953	0.0
3	0.0	0.0	0.0	0.14662067937779438	0.0
4	0.0	0.0	0.0	0.20739480972682717	0.0
5	0.0	0.0	0.0	0.3553573932029732	0.0
6	0.0	0.0	0.0	0.4849570784911489	0.0
7	0.0	0.0	0.0	0.5763478102420477	0.0
8	0.0	0.0	0.0	0.8452936421643218	0.0
9	0.0	0.0	0.0	0.9540585006623002	0.0
10	0.0	0.0	0.0	1.162406768564272	0.0
11	0.0	0.0	0.0	1.3609026353230829	0.0
12	0.0	0.0	0.0	1.5379633497740128	0.0
13	0.0	0.0	0.0	1.6041757230479445	0.0
14	0.0	0.0	0.0	1.629495334587896	0.0
15	0.0	0.0	0.0	1.679816738276084	0.0
16	0.0	0.0	0.0	1.7759041343712139	0.0
17	0.0	0.0	0.0	1.8953335991245135	0.0
18	0.0	0.0	0.0	2.0507825949388323	0.0
19	0.0	0.0	0.0	2.1288955387691986	0.0
20	3.5313265746096914E-5	0.0	0.0	2.222334439933371	0.0
21	3.5313265746096914E-5	0.0	0.0	2.3383385179092993	0.0
22	3.5313265746096914E-5	0.0	0.0	2.4655722143924863	0.0
23	3.5313265746096914E-5	0.0	0.0	2.594748140491709	0.0
24	3.5313265746096914E-5	0.0	0.0	2.7012529499819373	0.0
25	3.5313265746096914E-5	0.0	0.0	2.790207066396355	0.0
26	3.5313265746096914E-5	0.0	0.0	2.88082090630084	0.0
27	3.5313265746096914E-5	0.0	0.0	2.965396177762742	0.0
28	3.5313265746096914E-5	0.0	0.0	3.061518887123618	0.0
29	3.5313265746096914E-5	0.0	0.0	3.161455429185072	0.0
30	3.5313265746096914E-5	0.0	0.0	3.288512559339529	0.0
31	3.5313265746096914E-5	0.0	0.0	3.409884253708864	0.0
32	3.5313265746096914E-5	0.0	0.0	3.5209444744803386	0.0
33	3.5313265746096914E-5	0.0	0.0	3.628826501334665	0.0
34	3.5313265746096914E-5	0.0	0.0	3.7459959170802146	0.0
35	3.5313265746096914E-5	0.0	0.0	3.886825220875649	0.0
36	3.5313265746096914E-5	0.0	0.0	4.0175549306677	0.0
37	3.5313265746096914E-5	0.0	0.0	4.144894566948125	0.0
38	3.5313265746096914E-5	0.0	0.0	4.279014350251801	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	20	7.858435E-4	44.000004	32
ACGATTC	35	1.447097E-7	44.0	29
GTACGTC	30	2.5290356E-6	44.0	38
CGTTTTT	19450	0.0	42.993317	1
TATAGCG	150	0.0	42.533333	1
TATACGG	385	0.0	41.142857	2
CGGTCTA	375	0.0	40.48	31
CGACAGG	305	0.0	40.393444	2
TACGGGC	390	0.0	40.05128	4
TAGAACG	100	0.0	39.600002	1
ATACGGG	860	0.0	38.883724	3
GCGATCG	40	4.127396E-7	38.500004	9
AGGGCGC	515	0.0	38.446598	6
TAGGGCG	980	0.0	38.163265	5
GGTCGAC	75	0.0	38.13333	9
CGGGATA	630	0.0	38.06349	6
ACGGGAT	1155	0.0	37.714287	5
TTAGCGG	800	0.0	37.675003	2
GGGCGAT	4110	0.0	37.523113	7
CGTAAGG	440	0.0	37.5	2
>>END_MODULE