##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549133_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	351651
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4807550668134	31.0	31.0	33.0	30.0	34.0
2	31.903927473546215	31.0	31.0	34.0	30.0	34.0
3	32.01595900480874	33.0	31.0	34.0	30.0	34.0
4	35.74186622531999	37.0	35.0	37.0	35.0	37.0
5	35.65541403266307	37.0	35.0	37.0	33.0	37.0
6	35.69823205393985	37.0	35.0	37.0	33.0	37.0
7	35.96279265521782	37.0	35.0	37.0	35.0	37.0
8	35.9450705386875	37.0	35.0	37.0	35.0	37.0
9	37.714014747576435	39.0	38.0	39.0	35.0	39.0
10	37.26189034013838	39.0	37.0	39.0	34.0	39.0
11	37.01598175463741	39.0	37.0	39.0	33.0	39.0
12	36.458352173035195	39.0	35.0	39.0	32.0	39.0
13	36.22503277397192	38.0	35.0	39.0	32.0	39.0
14	37.28655115441162	39.0	35.0	41.0	32.0	41.0
15	37.401156260041915	39.0	35.0	41.0	32.0	41.0
16	37.515067495897924	39.0	35.0	41.0	33.0	41.0
17	37.41240605031693	39.0	35.0	41.0	32.0	41.0
18	37.33530119351288	39.0	35.0	41.0	32.0	41.0
19	37.27701044501509	39.0	35.0	41.0	32.0	41.0
20	37.12615064367797	39.0	35.0	41.0	32.0	41.0
21	37.00999570596984	39.0	35.0	41.0	32.0	41.0
22	36.88052358730673	38.0	35.0	41.0	32.0	41.0
23	36.793664741462415	38.0	35.0	41.0	31.0	41.0
24	36.8839758738067	38.0	35.0	41.0	32.0	41.0
25	36.85925249750463	38.0	35.0	41.0	32.0	41.0
26	36.61459515258026	38.0	35.0	41.0	31.0	41.0
27	36.62712746444628	38.0	35.0	41.0	31.0	41.0
28	36.60128365908244	38.0	35.0	41.0	31.0	41.0
29	36.53683055074492	38.0	35.0	41.0	31.0	41.0
30	36.44152014355142	38.0	35.0	41.0	31.0	41.0
31	36.29282726339467	38.0	35.0	41.0	30.0	41.0
32	36.15263997543018	38.0	35.0	41.0	30.0	41.0
33	35.93973854759406	38.0	35.0	41.0	30.0	41.0
34	35.735453617364946	38.0	35.0	41.0	29.0	41.0
35	35.53644949111477	38.0	35.0	41.0	27.0	41.0
36	35.40901063838863	38.0	35.0	40.0	27.0	41.0
37	35.340434123605505	38.0	34.0	40.0	26.0	41.0
38	35.261057127663506	38.0	34.0	40.0	26.0	41.0
39	35.1893525114389	38.0	34.0	40.0	26.0	41.0
40	35.05707932012137	38.0	34.0	40.0	25.0	41.0
41	35.01382620837137	38.0	34.0	40.0	25.0	41.0
42	34.99780179780521	38.0	34.0	40.0	25.0	41.0
43	34.88923677168556	38.0	34.0	40.0	24.0	41.0
44	34.786177204102934	37.0	34.0	40.0	24.0	41.0
45	34.68410725406724	37.0	34.0	40.0	24.0	41.0
46	34.63214095793841	37.0	34.0	40.0	24.0	41.0
47	34.5603851545993	37.0	34.0	40.0	24.0	41.0
48	34.47113188928796	37.0	34.0	40.0	23.0	41.0
49	34.38366448552684	36.0	34.0	40.0	24.0	41.0
50	34.24260986034449	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	11.0
16	26.0
17	74.0
18	138.0
19	258.0
20	498.0
21	789.0
22	1155.0
23	1660.0
24	2474.0
25	3665.0
26	5434.0
27	6576.0
28	7165.0
29	7704.0
30	8513.0
31	9973.0
32	12033.0
33	15803.0
34	26978.0
35	41647.0
36	23799.0
37	31443.0
38	50787.0
39	93044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.236083503246114	23.788073971067906	29.609470753673385	15.366371772012592
2	31.35665759517249	25.60635402714623	30.142101117300964	12.894887260380319
3	28.599093988073403	24.844234766856914	33.49826959115714	13.058401653912544
4	25.61118836573762	27.360081444386626	32.834543339845474	14.194186850030285
5	23.581903648788145	32.094036416788235	30.542498101811173	13.781561832612448
6	21.376023386823867	40.93063861612792	27.083671026102586	10.609666970945625
7	87.45147888104968	3.54868889893673	7.357578963233433	1.6422532567801598
8	88.46782747667432	2.5912054849836914	7.321179237368868	1.619787800973124
9	82.86653528640613	6.183972176959542	8.559054289622381	2.3904382470119523
10	38.513184947575866	39.30260400226361	12.777725642753754	9.406485407406775
11	37.901498929336185	21.22672763620749	25.83669604238293	15.03507739207339
12	35.59779440411089	20.90823003489255	27.151636139240328	16.34233942175623
13	23.663803031983417	30.38609302973687	28.220024967936958	17.730078970342756
14	17.304941547158972	32.821177815504576	31.450785011275386	18.423095626061066
15	17.56030837392756	23.967513244665874	41.44620660825648	17.025971773150083
16	20.63011337945861	22.037474655269	38.34512058831057	18.987291376961817
17	20.343181165416848	20.99752311240406	30.74127472977469	27.9180209924044
18	22.419103031130298	23.419242373830873	32.47793977551607	21.683714819522766
19	26.449235179197554	24.736173080696485	27.99082044413353	20.823771295972428
20	28.835123460476435	23.447110913945927	27.61772325402174	20.10004237155589
21	24.221742580001195	25.177519756804333	29.613451973689823	20.98728568950465
22	23.39734566374047	23.198284662918635	29.23552044498665	24.16884922835425
23	21.55460954184689	27.625116948337986	29.041578155614516	21.778695354200615
24	21.429485484187445	23.141125718396932	35.64244094286665	19.78694785454897
25	19.947902892356343	25.548626336907898	32.70060372357821	21.80286704715755
26	19.696232912745877	30.027783228257558	29.21191749774634	21.064066361250216
27	19.598124276626542	28.84422339194258	30.0300582111241	21.52759412030678
28	18.139007140602473	26.874656975239652	35.23834711119832	19.747988772959555
29	19.8967157778593	23.681434149199063	34.67671071602242	21.745139356919218
30	21.410148129821895	25.58644792706405	32.18418261287469	20.81922133023936
31	26.11737205354172	25.409852382049248	27.964942514026692	20.50783305038234
32	27.755359717447124	25.005758550380918	28.516910232019814	18.72197150015214
33	25.680006597450312	26.27861146420741	27.847780896400128	20.193601041942152
34	21.51479734168252	26.212636961077884	30.95540749208733	21.317158205152268
35	21.545509610380748	26.16941228661372	31.339595223673474	20.945482879332065
36	25.70474703612388	27.60634833968907	27.347000292904045	19.341904331283004
37	22.32469124216908	30.158025997366707	27.98797671555036	19.52930604491385
38	22.027237232369593	30.188738266064934	26.517200292335296	21.266824209230172
39	22.891446348794688	28.65369357687025	27.23780111531035	21.217058959024715
40	23.76532414240255	26.375013863176843	27.652701115594724	22.206960878825882
41	19.90496259075049	26.04371948323764	28.079544775928404	25.971773150083465
42	22.383840796699	25.989973013015742	25.765887200662018	25.86029898962324
43	22.179092338710824	26.108272122075583	27.742562938822868	23.97007260039073
44	21.13828767727093	28.520322706319618	28.065894878729196	22.27549473768026
45	20.239385072131174	30.60278514777436	26.602227776972054	22.555602003122413
46	21.965243949256507	29.80824738163691	27.328231684255126	20.898276984851456
47	21.754808034101995	26.25472414410879	29.631367463763787	22.35910035802543
48	22.686129145089875	23.652996863367374	31.186318253040657	22.474555738502094
49	21.333083085218014	24.832859852524237	31.16214656008372	22.67191050217403
50	19.76306053445035	29.17011468757376	29.30064182954122	21.766182948434672
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57.0
1	263.5
2	470.0
3	2527.5
4	4585.0
5	3320.5
6	2056.0
7	2017.5
8	1979.0
9	1934.5
10	1890.0
11	1905.0
12	1920.0
13	1747.5
14	1575.0
15	1450.0
16	1325.0
17	1319.5
18	1314.0
19	1292.5
20	1271.0
21	1234.0
22	1197.0
23	1579.0
24	1961.0
25	2168.5
26	2376.0
27	3190.5
28	4005.0
29	4962.5
30	5920.0
31	6257.0
32	6594.0
33	7811.0
34	9028.0
35	10306.5
36	11585.0
37	12312.5
38	13040.0
39	15046.5
40	17053.0
41	20710.0
42	24367.0
43	27029.0
44	29691.0
45	29982.5
46	30274.0
47	29028.0
48	27782.0
49	26238.5
50	24695.0
51	22598.5
52	20502.0
53	19900.0
54	19298.0
55	18423.0
56	17548.0
57	16491.5
58	15435.0
59	15244.5
60	15054.0
61	13427.5
62	11801.0
63	10164.5
64	8528.0
65	7202.0
66	5876.0
67	4694.0
68	3512.0
69	2842.0
70	2172.0
71	1829.0
72	1486.0
73	1292.5
74	1099.0
75	800.5
76	502.0
77	464.5
78	427.0
79	322.0
80	217.0
81	158.0
82	99.0
83	75.0
84	51.0
85	35.0
86	19.0
87	12.5
88	6.0
89	5.5
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	351651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.04329008022158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.02670928936465	18.937526126756357
2	6.366064480851333	3.4431865685011562
3	3.2587436118530357	2.6438144637723195
4	2.50793917853162	2.7129170683433292
5	1.9716502975877515	2.665995546721039
6	1.5941449872762834	2.5866555192506206
7	1.4174851206124208	2.6833422910783704
8	1.2019180214094933	2.600305416449832
9	1.1030726198237608	2.684764155369955
>10	10.410313571263329	55.906850826529706
>50	0.13354644682327704	2.113459083011281
>100	0.007360827777660939	0.40380945881001334
>500	0.0	0.0
>1k	0.0010515468253801342	0.6173734754060133
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2171	0.6173734754060133	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	476	0.13536148055884953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025877930106838882	0.0
2	0.0	0.0	0.0	0.09583365325279894	0.0
3	0.0	0.0	0.0	0.14872700489974436	0.0
4	0.0	0.0	0.0	0.22209520234550734	0.0
5	0.0	0.0	0.0	0.39442515448555526	0.0
6	0.0	0.0	0.0	0.570451953783723	0.0
7	0.0	0.0	0.0	0.6913104185684101	0.0
8	0.0	0.0	0.0	1.0354015771318723	0.0
9	0.0	0.0	0.0	1.2014753263889482	0.0
10	0.0	0.0	0.0	1.4594015088823862	0.0
11	0.0	0.0	0.0	1.6627281025789775	0.0
12	0.0	0.0	0.0	1.8686140520004209	0.0
13	0.0	0.0	0.0	1.9399916394379655	0.0
14	0.0	0.0	0.0	1.9675758066947058	0.0
15	0.0	0.0	0.0	2.0144973283169962	0.0
16	0.0	0.0	0.0	2.1094778629948445	0.0
17	0.0	0.0	0.0	2.2212363963133903	0.0
18	0.0	0.0	0.0	2.39527258560334	0.0
19	0.0	0.0	0.0	2.4729063759238565	0.0
20	0.0	0.0	0.0	2.5710150120431905	0.0
21	2.843728583169108E-4	0.0	0.0	2.6822047996451026	0.0
22	2.843728583169108E-4	0.0	0.0	2.8010726544215716	0.0
23	2.843728583169108E-4	0.0	0.0	2.925912339222695	0.0
24	2.843728583169108E-4	0.0	0.0	3.0240209753420295	0.0
25	2.843728583169108E-4	0.0	0.0	3.101939138520863	0.0
26	2.843728583169108E-4	0.0	0.0	3.181847911707915	0.0
27	2.843728583169108E-4	0.0	0.0	3.264316040619819	0.0
28	2.843728583169108E-4	0.0	0.0	3.3501966438315263	0.0
29	2.843728583169108E-4	0.0	0.0	3.453992737117199	0.0
30	2.843728583169108E-4	0.0	0.0	3.567457507585646	0.0
31	2.843728583169108E-4	0.0	0.0	3.669831736579734	0.0
32	2.843728583169108E-4	0.0	0.0	3.7736278298654065	0.0
33	2.843728583169108E-4	0.0	0.0	3.8717364659847404	0.0
34	2.843728583169108E-4	0.0	0.0	3.9803668978618005	0.0
35	2.843728583169108E-4	0.0	0.0	4.104353464087974	0.0
36	2.843728583169108E-4	0.0	0.0	4.20502145593216	0.0
37	2.843728583169108E-4	0.0	0.0	4.312230023517635	0.0
38	2.843728583169108E-4	0.0	0.0	4.433941606877273	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	40	8.283678E-9	44.0	24
ATTAGCG	40	8.283678E-9	44.0	1
ATAATCG	20	7.849022E-4	44.0	25
CGTTGAT	40	8.283678E-9	44.0	25
ATTAACG	20	7.849022E-4	44.0	1
GCGGGTC	35	1.4422585E-7	44.0	5
CGTAAGG	35	1.4422585E-7	44.0	2
CATCGCA	20	7.849022E-4	44.0	43
CGTCGGG	20	7.849022E-4	44.0	3
ATACGGA	20	7.849022E-4	44.0	3
TCGACGG	25	4.4365202E-5	44.0	2
TATCGAG	20	7.849022E-4	44.0	1
ATCGATA	20	7.849022E-4	44.0	28
AGTAACG	20	7.849022E-4	44.0	1
AATCGAT	20	7.849022E-4	44.0	27
GTACGAG	25	4.4365202E-5	44.0	1
TAATCGA	20	7.849022E-4	44.0	26
ACGCGGA	20	7.849022E-4	44.0	11
CGTTTTT	1885	0.0	41.782494	1
CGGGATG	170	0.0	41.411762	6
>>END_MODULE