FastQCFastQC Report
Sat 18 Jun 2016
SRR3549132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994334
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79870.803251221420569No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20180.20294991421393616No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA13840.13918864285039032No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA12880.1295339393000742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAG650.044.0000041
ACGCGAT207.85597E-444.010
TCGTTAG351.4458237E-744.01
CAAACGT207.85597E-444.041
CGAACAA302.5273712E-644.025
ACGCACC207.85597E-444.041
GCGCGAT207.85597E-444.039
CGGCATA302.5273712E-644.036
CGCGCAT254.4424058E-544.042
CGCGATT207.85597E-444.011
CGACAGT254.4424058E-544.034
TGTGCGA207.85597E-444.015
TCGATAG254.4424058E-544.01
CCGTCCG207.85597E-444.035
ATCGTAG254.4424058E-544.01
TAGCACG254.4424058E-544.01
TTGCGTA454.802132E-1044.01
TATTACG254.4424058E-544.01
CGAATGC207.85597E-444.043
GTTCACG207.85597E-444.042
Produced by FastQC (version 0.11.5)