##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549132_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	994334
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.489891726522476	31.0	31.0	33.0	30.0	34.0
2	31.907100632181944	31.0	31.0	34.0	30.0	34.0
3	32.00308648804124	33.0	31.0	34.0	30.0	34.0
4	35.74805145956992	37.0	35.0	37.0	35.0	37.0
5	35.664524194083675	37.0	35.0	37.0	33.0	37.0
6	35.718869112390806	37.0	35.0	37.0	33.0	37.0
7	35.97326351105363	37.0	35.0	37.0	35.0	37.0
8	35.95032554453534	37.0	35.0	37.0	35.0	37.0
9	37.72027206150046	39.0	38.0	39.0	35.0	39.0
10	37.2678265049772	39.0	37.0	39.0	34.0	39.0
11	37.01900468051983	39.0	37.0	39.0	33.0	39.0
12	36.41816431903163	38.0	35.0	39.0	32.0	39.0
13	36.1357632344866	38.0	35.0	39.0	32.0	39.0
14	37.19375380908226	39.0	35.0	41.0	32.0	41.0
15	37.33992602083404	39.0	35.0	41.0	32.0	41.0
16	37.487886364139214	39.0	35.0	41.0	33.0	41.0
17	37.40388843185489	39.0	35.0	41.0	33.0	41.0
18	37.32320326972627	39.0	35.0	41.0	32.0	41.0
19	37.210695802416495	38.0	35.0	41.0	32.0	41.0
20	37.0418843165375	38.0	35.0	41.0	32.0	41.0
21	36.91991021125698	38.0	35.0	41.0	32.0	41.0
22	36.800125511146156	38.0	35.0	41.0	32.0	41.0
23	36.724079635213116	38.0	35.0	41.0	32.0	41.0
24	36.75892104665032	38.0	35.0	41.0	32.0	41.0
25	36.73144436376509	38.0	35.0	41.0	32.0	41.0
26	36.50271940816667	38.0	35.0	41.0	31.0	41.0
27	36.48597956018803	38.0	35.0	41.0	31.0	41.0
28	36.47200940529037	38.0	35.0	41.0	31.0	41.0
29	36.43377577353284	38.0	35.0	41.0	31.0	41.0
30	36.343168392109696	38.0	35.0	40.0	31.0	41.0
31	36.18350172074977	38.0	35.0	40.0	30.0	41.0
32	35.9739876138199	38.0	35.0	40.0	30.0	41.0
33	35.7478302059469	38.0	35.0	40.0	29.0	41.0
34	35.50462420072129	38.0	35.0	40.0	27.0	41.0
35	35.31858108040156	38.0	34.0	40.0	27.0	41.0
36	35.1270900924639	38.0	34.0	40.0	25.0	41.0
37	35.03813306192889	38.0	34.0	40.0	25.0	41.0
38	34.988271546582936	38.0	34.0	40.0	25.0	41.0
39	34.90289279055126	38.0	34.0	40.0	24.0	41.0
40	34.763846956857556	37.0	34.0	40.0	24.0	41.0
41	34.745373285033	37.0	34.0	40.0	24.0	41.0
42	34.72083424684261	37.0	34.0	40.0	24.0	41.0
43	34.612374715135964	37.0	34.0	40.0	23.0	41.0
44	34.49511934621566	37.0	34.0	40.0	23.0	41.0
45	34.38650493697289	37.0	34.0	40.0	23.0	41.0
46	34.33713118529589	37.0	34.0	40.0	23.0	41.0
47	34.28723447050991	36.0	33.0	40.0	23.0	41.0
48	34.1885985996657	36.0	33.0	40.0	23.0	41.0
49	34.12095231582144	36.0	33.0	40.0	23.0	41.0
50	33.96329402393964	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	3.0
14	9.0
15	28.0
16	50.0
17	157.0
18	353.0
19	774.0
20	1352.0
21	2185.0
22	3279.0
23	5019.0
24	7319.0
25	11042.0
26	16403.0
27	20572.0
28	21772.0
29	22442.0
30	24476.0
31	28778.0
32	34955.0
33	45110.0
34	79070.0
35	129151.0
36	66106.0
37	84488.0
38	134607.0
39	254830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.414293386326932	23.452180052175628	28.924486138460516	16.209040423036928
2	31.651638181938868	25.135014994961452	30.240040067019734	12.97330675607995
3	28.435515631568464	24.510074079735784	33.89967556173278	13.154734726962971
4	25.563643604664026	27.20765859359129	33.110705255980385	14.117992545764302
5	22.536592332153983	31.653951287997796	31.787709160101134	14.021747219747088
6	20.433274935786162	41.275466794859675	28.02006166941893	10.271196599935232
7	85.39535005340258	4.026514229625056	8.90234066219198	1.6757950547803855
8	86.39833295452031	3.1241011571564488	8.700195306607236	1.7773705817160028
9	80.95398528059988	6.252828526430757	10.111089432725825	2.6820967602435397
10	40.323673936524344	35.57728087342885	14.47692626421303	9.622118925833774
11	39.941910866972265	20.772798677305612	25.43793131885262	13.847359136869503
12	37.734905977267196	20.4579145438052	26.913391274963942	14.893788203963659
13	22.055767981382512	33.73242793668928	27.72076585935913	16.491038222569077
14	16.478265854330637	35.96719009910151	30.02492120353925	17.5296228430286
15	15.566600357626312	24.2836913954466	44.08418096937247	16.065527277554626
16	18.09090305671937	20.13679508092854	42.472247755784274	19.300054106567814
17	17.62777899579015	20.687817172097102	31.646509120677763	30.037894711434994
18	20.471290331015535	23.52318235120191	34.68130426999379	21.32422304778877
19	25.65757582462231	24.136859445618878	29.150567113263754	21.05499761649506
20	28.333839534804202	22.822914634318046	29.243694774592843	19.599551056284913
21	22.866863649437715	25.943696987129073	29.967898110695202	21.221541252738014
22	23.255566037166588	23.807593826621638	28.802997785452373	24.1338423507594
23	19.970352014514237	27.71352483169639	29.322742659911054	22.993380493878316
24	19.5374994720084	22.618154463188425	38.58371533106582	19.260630733737354
25	18.340215661940558	26.239070573871555	34.292903591750864	21.127810172437027
26	18.01527454557523	31.16769616647927	29.55194129940241	21.265087988543087
27	18.495797186860752	30.47597688503058	30.963841123807494	20.06438480430117
28	16.84956966170321	27.39411505590677	37.37436314156008	18.381952140829945
29	17.789394710429292	23.308365197207376	36.804031643290884	22.098208449072445
30	19.041790786596856	28.346511333213993	32.204973379166354	20.406724501022794
31	25.572091470270554	26.5394726520465	27.76008866236094	20.128347215322016
32	26.00725711883532	26.289858337339368	28.525123348894837	19.177761194930476
33	24.78684224817818	28.12083263772535	27.188148046833362	19.904177067263113
34	19.609205759835227	26.9534180667663	30.58016722751108	22.857208945887397
35	20.88121295258937	24.852916625600656	31.02046193733695	23.245408484473025
36	25.991367085908756	25.82210806429228	27.4938803259267	20.69264452387226
37	20.91942948747604	30.034978186404164	29.202762854332647	19.842829471787145
38	19.948427791868728	30.2903249813443	27.563374077523246	22.197873149263728
39	20.47672110176259	29.29860590103527	27.94292461084505	22.2817483863571
40	22.129385095953673	27.17376656133653	27.011748567382792	23.685099775327004
41	18.292746702818167	25.748289810063824	28.928508931606483	27.03045455551153
42	21.292342412106997	26.469073771992107	26.21443096585252	26.024152850048377
43	21.999448877339002	27.10467508905458	27.137360283365545	23.758515750240864
44	20.234951233690087	28.574201425275614	28.37386632660655	22.816981014427746
45	17.789998129401187	32.56873444939025	25.841115761906963	23.8001516593016
46	20.579201757156046	31.04007305392353	27.87443655753499	20.50628863138543
47	20.770284431589385	26.58181254990778	29.4554948337279	23.192408184774933
48	21.811785577079736	24.368572330826463	31.09317392345027	22.726468168643535
49	20.337834168398146	24.86699640161153	31.850062453863593	22.945106976126734
50	19.242829874066462	29.25043295311233	29.30182413555204	22.204913037269165
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	336.0
1	871.5
2	1407.0
3	8414.5
4	15422.0
5	11298.5
6	7175.0
7	6972.5
8	6770.0
9	6743.0
10	6716.0
11	6525.5
12	6335.0
13	5874.0
14	5413.0
15	5158.5
16	4904.0
17	4359.5
18	3815.0
19	3602.5
20	3390.0
21	3388.0
22	3386.0
23	3869.5
24	4353.0
25	5566.5
26	6780.0
27	9425.0
28	12070.0
29	13857.0
30	15644.0
31	17900.5
32	20157.0
33	22888.0
34	25619.0
35	28123.5
36	30628.0
37	34448.5
38	38269.0
39	43702.0
40	49135.0
41	60427.5
42	71720.0
43	79888.5
44	88057.0
45	88178.5
46	88300.0
47	82557.5
48	76815.0
49	77757.5
50	78700.0
51	73582.0
52	68464.0
53	61980.5
54	55497.0
55	50680.5
56	45864.0
57	42316.0
58	38768.0
59	36916.5
60	35065.0
61	31475.5
62	27886.0
63	23462.5
64	19039.0
65	15259.5
66	11480.0
67	9795.0
68	8110.0
69	6646.0
70	5182.0
71	4241.0
72	3300.0
73	2605.0
74	1910.0
75	1628.0
76	1346.0
77	968.0
78	590.0
79	448.5
80	307.0
81	198.5
82	90.0
83	66.0
84	42.0
85	40.5
86	39.0
87	25.0
88	11.0
89	12.0
90	13.0
91	10.5
92	8.0
93	7.5
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	994334.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.288813186429767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.87991031017843	16.00379764445545
2	6.564494342469765	2.663715987554883
3	2.18160722758448	1.327866644598794
4	1.225179107844246	0.9942972015587437
5	0.8580507260693558	0.8704415442850789
6	0.6502825414717053	0.7916076601389717
7	0.535458111983527	0.7604666722234518
8	0.4696251582808548	0.7622509683206219
9	0.37859369116166103	0.6913095006185843
>10	5.64837551648171	27.5967016471742
>50	1.8997706851384597	27.617432464448065
>100	0.7056434408630646	18.5131712848391
>500	0.0010030468242545338	0.11701612039861585
>1k	0.0015045702363818008	0.47722226493000725
>5k	5.015234121272669E-4	0.8127023944554301
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7987	0.803251221420569	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2018	0.20294991421393616	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	1384	0.13918864285039032	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1288	0.1295339393000742	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06054303684677382	0.0
2	0.0	0.0	0.0	0.22457242737349825	0.0
3	0.0	0.0	0.0	0.30925222309606226	0.0
4	0.0	0.0	0.0	0.41595681129278494	0.0
5	0.0	0.0	0.0	0.6784440640670036	0.0
6	0.0	0.0	0.0	0.893562927547484	0.0
7	0.0	0.0	0.0	1.0435125420633309	0.0
8	0.0	0.0	0.0	1.4416684936852204	0.0
9	0.0	0.0	0.0	1.604993895411401	0.0
10	0.0	0.0	0.0	1.909217627075007	0.0
11	0.0	0.0	0.0	2.176632801453033	0.0
12	0.0	0.0	0.0	2.426548825646111	0.0
13	0.0	0.0	0.0	2.5278226430957806	0.0
14	0.0	0.0	0.0	2.5613123960359396	0.0
15	0.0	0.0	0.0	2.620950304424871	0.0
16	0.0	0.0	0.0	2.7600383774466124	0.0
17	0.0	0.0	0.0	2.932716773237162	0.0
18	0.0	0.0	0.0	3.179414562913468	0.0
19	0.0	0.0	0.0	3.28843225716912	0.0
20	0.0	0.0	0.0	3.406702375660492	0.0
21	0.0	0.0	0.0	3.551623498743883	0.0
22	0.0	0.0	0.0	3.7143454814981687	0.0
23	0.0	0.0	0.0	3.8792800004827352	0.0
24	0.0	0.0	0.0	4.009618498412002	0.0
25	0.0	0.0	0.0	4.119641890954147	0.0
26	0.0	0.0	0.0	4.229263004181694	0.0
27	0.0	0.0	0.0	4.340493234667627	0.0
28	0.0	0.0	0.0	4.457355375557911	0.0
29	0.0	0.0	0.0	4.582363672568775	0.0
30	0.0	0.0	0.0	4.743074258750078	0.0
31	0.0	0.0	0.0	4.888900510291311	0.0
32	0.0	0.0	0.0	5.016020773703806	0.0
33	0.0	0.0	0.0	5.139218813798985	0.0
34	0.0	0.0	0.0	5.279714864421814	0.0
35	0.0	0.0	0.0	5.451085852439925	0.0
36	0.0	0.0	0.0	5.600331478155227	0.0
37	0.0	0.0	0.0	5.751588500443513	0.0
38	0.0	0.0	0.0	5.894397657125272	1.0056982864912595E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGAG	65	0.0	44.000004	1
ACGCGAT	20	7.85597E-4	44.0	10
TCGTTAG	35	1.4458237E-7	44.0	1
CAAACGT	20	7.85597E-4	44.0	41
CGAACAA	30	2.5273712E-6	44.0	25
ACGCACC	20	7.85597E-4	44.0	41
GCGCGAT	20	7.85597E-4	44.0	39
CGGCATA	30	2.5273712E-6	44.0	36
CGCGCAT	25	4.4424058E-5	44.0	42
CGCGATT	20	7.85597E-4	44.0	11
CGACAGT	25	4.4424058E-5	44.0	34
TGTGCGA	20	7.85597E-4	44.0	15
TCGATAG	25	4.4424058E-5	44.0	1
CCGTCCG	20	7.85597E-4	44.0	35
ATCGTAG	25	4.4424058E-5	44.0	1
TAGCACG	25	4.4424058E-5	44.0	1
TTGCGTA	45	4.802132E-10	44.0	1
TATTACG	25	4.4424058E-5	44.0	1
CGAATGC	20	7.85597E-4	44.0	43
GTTCACG	20	7.85597E-4	44.0	42
>>END_MODULE