FastQCFastQC Report
Sat 18 Jun 2016
SRR3549130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725206
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134200.7778781200621839No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32460.18815144394350589No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA27240.15789418770859828No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA26880.15580748038205292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC302.5284571E-644.014
CGTCTAC207.85758E-444.038
CGCGAAA254.443771E-544.032
TATAGCG1550.044.01
CGCACTA302.5284571E-644.028
TGCGTCG254.443771E-544.01
CGTTTTT86800.042.7580641
GCGTTAG950.041.684211
CGTTAGG2400.041.252
CGGTCTA2300.041.13043631
TCTTACG700.040.8571431
CGTTAAG700.040.85714316
TACGTCT700.040.85714344
ACGGTCT2350.040.25531830
ACGGTAC557.8216544E-1140.013
ATCGAGA501.3478711E-939.623
TACGGGA6900.039.5362324
TGCGTAG1400.039.2857131
AACGGTC452.3521352E-839.1111128
TATGACG850.038.823531
Produced by FastQC (version 0.11.5)