FastQCFastQC Report
Sat 18 Jun 2016
SRR3549128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3662882
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343890.9388508829932277No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA53240.14535002765581856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC254.444753E-544.031
CGTTTTT235200.042.653061
CACGACC2750.040.827
CGACGGT3700.039.2432428
TACGGGA22000.039.24
CCTTACG452.3534085E-839.1111138
GGCGATA13400.039.0746278
AGGGCGA31200.039.0641026
GGGCGAT56250.038.2897767
AGGGATC69600.038.025866
CTCGCGA357.2945677E-637.71428725
CGTAAGG4150.037.6385542
AATACGG4150.037.6385542
TCGTAAG2400.037.5833321
CGAGGGA34650.037.4603164
TAGGGAT92400.037.3333325
GCGATAA4600.037.3043489
ATGGGAC42050.037.303215
TGTTGCG2950.037.2881361
TAGGGAC49450.037.28215
Produced by FastQC (version 0.11.5)