##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3662882
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5210427199129	31.0	31.0	33.0	30.0	34.0
2	31.932717734286825	31.0	31.0	34.0	30.0	34.0
3	32.00108138891725	33.0	31.0	34.0	30.0	34.0
4	35.747630963814835	37.0	35.0	37.0	35.0	37.0
5	35.6722367796724	37.0	35.0	37.0	33.0	37.0
6	35.72113379573789	37.0	35.0	37.0	33.0	37.0
7	36.022178983652765	37.0	35.0	37.0	35.0	37.0
8	36.01354370684068	37.0	35.0	37.0	35.0	37.0
9	37.78680749202404	39.0	38.0	39.0	35.0	39.0
10	37.303103949294574	39.0	37.0	39.0	34.0	39.0
11	37.1016314475869	39.0	37.0	39.0	33.0	39.0
12	36.71662587001165	39.0	35.0	39.0	33.0	39.0
13	36.546423826920986	39.0	35.0	39.0	32.0	39.0
14	37.67197578300366	40.0	36.0	41.0	33.0	41.0
15	37.754724285412415	40.0	36.0	41.0	33.0	41.0
16	37.83298206166619	40.0	36.0	41.0	33.0	41.0
17	37.74713845545666	40.0	36.0	41.0	33.0	41.0
18	37.61252996957041	39.0	36.0	41.0	33.0	41.0
19	37.521694392557556	39.0	36.0	41.0	33.0	41.0
20	37.3610722376533	39.0	35.0	41.0	32.0	41.0
21	37.24546763996219	39.0	35.0	41.0	32.0	41.0
22	37.153074000199844	39.0	35.0	41.0	32.0	41.0
23	37.05422942917626	39.0	35.0	41.0	32.0	41.0
24	37.11673703930402	39.0	35.0	41.0	32.0	41.0
25	37.10279473922447	39.0	35.0	41.0	32.0	41.0
26	36.88271503149706	39.0	35.0	41.0	32.0	41.0
27	36.859074357295704	39.0	35.0	41.0	31.0	41.0
28	36.83137867941146	39.0	35.0	41.0	31.0	41.0
29	36.78740265179167	39.0	35.0	41.0	31.0	41.0
30	36.67852636257461	39.0	35.0	41.0	31.0	41.0
31	36.5337919703665	39.0	35.0	41.0	31.0	41.0
32	36.34493357962391	39.0	35.0	41.0	30.0	41.0
33	36.08533253323476	39.0	35.0	41.0	30.0	41.0
34	35.82669357080026	38.0	35.0	41.0	28.0	41.0
35	35.597119972742775	38.0	35.0	41.0	27.0	41.0
36	35.43997513433411	38.0	35.0	41.0	25.0	41.0
37	35.369718434828094	38.0	35.0	41.0	25.0	41.0
38	35.2897183147041	38.0	34.0	41.0	25.0	41.0
39	35.17691697412038	38.0	34.0	40.0	24.0	41.0
40	35.07799514153063	38.0	34.0	40.0	24.0	41.0
41	35.02969956444133	38.0	34.0	40.0	24.0	41.0
42	35.013222921186106	38.0	34.0	40.0	24.0	41.0
43	34.921965545163616	38.0	34.0	40.0	23.0	41.0
44	34.80006344730734	38.0	34.0	40.0	23.0	41.0
45	34.71845694182887	38.0	34.0	40.0	23.0	41.0
46	34.668922995608376	38.0	34.0	40.0	23.0	41.0
47	34.59991503957812	38.0	34.0	40.0	23.0	41.0
48	34.48009955002645	38.0	34.0	40.0	23.0	41.0
49	34.416784925094504	37.0	34.0	40.0	23.0	41.0
50	34.293057761620496	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	0.0
12	1.0
13	9.0
14	32.0
15	90.0
16	195.0
17	528.0
18	1187.0
19	2533.0
20	4580.0
21	7365.0
22	11443.0
23	17506.0
24	26113.0
25	40273.0
26	60028.0
27	76100.0
28	78965.0
29	79008.0
30	85901.0
31	98237.0
32	118036.0
33	150494.0
34	239207.0
35	374601.0
36	250124.0
37	329340.0
38	539082.0
39	1071901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.39454123829269	23.049991782427064	29.81739515496268	15.738071824317574
2	32.861637366423494	24.915763052154013	29.65233387261724	12.570265708805254
3	27.497364097451133	24.55844878431792	35.16111084113548	12.783076277095468
4	25.01612118544905	27.51205198529464	33.908954752023135	13.562872077233173
5	22.448525505326135	32.52859360470799	31.922759182523485	13.10012170744239
6	20.2016608779644	41.08398796357623	28.54126886970424	10.17308228875514
7	86.40534966728384	3.3457534258542863	8.935695990206618	1.3132009166552459
8	87.67178413063812	2.502291911123536	8.629134108060265	1.1967898501780838
9	81.74410204860544	6.452514713823705	9.78718397152843	2.016199266042422
10	31.473713867932407	45.578672750036716	14.323011224494811	8.62460215753606
11	31.415672140134465	22.440226029667347	31.20171493375981	14.942386896438379
12	29.948849021071382	22.149089159847353	31.380781581279443	16.521280237801818
13	22.3799183266073	29.328081002882428	31.318426310211468	16.973574360298805
14	17.157637073757765	32.01339819300758	33.23049991782427	17.598464815410377
15	17.137925819068155	25.76362001287511	40.64384274459292	16.45461142346382
16	20.229262094711213	24.489896207412635	37.94561222556446	17.335229472311696
17	19.99665836901107	23.013272062818295	32.52073094355756	24.469338624613076
18	20.966959896606006	24.231192814838153	35.26226070072692	19.53958658782893
19	24.544197710982772	24.570160873323246	31.33450654430036	19.551134871393618
20	25.96171539241504	25.1316586229095	30.555011054137154	18.351614930538304
21	23.04843563074104	25.207091028321415	32.89942182139637	18.84505151954117
22	22.35480149237677	23.236893790190347	32.119325711284176	22.28897900614871
23	19.356015290691865	27.23631282689423	32.321024810518054	21.086647071895843
24	18.974021003133597	25.310534164081727	37.100294249173196	18.615150583611484
25	18.863561534332803	26.91337040068449	34.32343711864046	19.89963094634225
26	19.315009328719846	30.113200479840735	30.79154611041251	19.780244081026908
27	18.94328018210797	29.13449027295993	32.83490977869339	19.087319766238718
28	17.219200618529342	27.200630541742814	37.02783764259946	18.55233119712838
29	18.78769231441253	24.51236485368625	36.09835097062914	20.60159186127208
30	20.10840098043016	27.13988602417441	33.34519102717478	19.406521968220652
31	23.80931190248553	26.46339685526315	30.688758196414735	19.038533045836584
32	24.480313589135548	26.680411763196304	31.206519893351736	17.63275475431641
33	23.51470781750545	27.290614330464376	30.103208347962067	19.091469504068108
34	20.40133971009713	27.368258109324845	31.374611576348897	20.855790604229128
35	20.53301198346002	26.861962793232212	32.00043026229073	20.604594961017035
36	24.26878616346363	27.606567724540405	29.620582918041038	18.504063193954924
37	20.729114396805574	30.35814421540197	30.109678662867108	18.803062724925347
38	20.189266266289767	30.298546335918005	28.95040025859419	20.56178713919804
39	20.826851643050475	29.258600195146883	30.255001389616154	19.65954677218649
40	22.812064379906314	27.315621961067816	29.247133814302508	20.625179844723363
41	19.650046056629726	27.023939073112373	30.10509211052936	23.22092275972854
42	21.70372946767054	27.223262993457066	28.604033654373794	22.4689738844986
43	22.049304345594535	26.80080876206222	28.902159556327504	22.24772733601574
44	20.518105688362333	29.056955697726544	29.955373937789968	20.469564676121152
45	19.57657931650542	31.11104862236894	27.691910359110665	21.62046170201497
46	20.848200952146424	30.29240363189423	28.936067282538723	19.923328133420622
47	21.004798953392438	27.802451730631784	30.49563158190736	20.697117734068417
48	21.199945835000964	26.58414876591711	31.29232664333713	20.923578755744792
49	20.451354971304017	26.219545155972813	32.40295483174178	20.926145040981392
50	20.0454450894132	29.903939029430926	30.14148421925686	19.909131661899018
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	703.0
1	2739.5
2	4776.0
3	34597.5
4	64419.0
5	46580.5
6	28742.0
7	27822.0
8	26902.0
9	26341.0
10	25780.0
11	25206.0
12	24632.0
13	23186.0
14	21740.0
15	20454.0
16	19168.0
17	18077.5
18	16987.0
19	17050.5
20	17114.0
21	17678.0
22	18242.0
23	21801.0
24	25360.0
25	31672.0
26	37984.0
27	44715.5
28	51447.0
29	64089.5
30	76732.0
31	87333.0
32	97934.0
33	112540.0
34	127146.0
35	139842.5
36	152539.0
37	166833.0
38	181127.0
39	194331.5
40	207536.0
41	234059.0
42	260582.0
43	283014.0
44	305446.0
45	311213.0
46	316980.0
47	302581.0
48	288182.0
49	274886.5
50	261591.0
51	244472.5
52	227354.0
53	206172.0
54	184990.0
55	168580.0
56	152170.0
57	136966.0
58	121762.0
59	111754.0
60	101746.0
61	88620.0
62	75494.0
63	63672.5
64	51851.0
65	42914.0
66	33977.0
67	27346.5
68	20716.0
69	17727.5
70	14739.0
71	11515.5
72	8292.0
73	6134.0
74	3976.0
75	3575.0
76	3174.0
77	2217.5
78	1261.0
79	1079.0
80	897.0
81	628.5
82	360.0
83	267.0
84	174.0
85	139.0
86	104.0
87	68.5
88	33.0
89	22.0
90	11.0
91	8.0
92	5.0
93	4.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3662882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.505599793999117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2580601732535	13.082018216684228
2	7.790615782547791	2.5717757251109417
3	2.5919080304338515	1.283429899595809
4	1.25474329565376	0.8284116272905792
5	0.7799858989027639	0.643706754612584
6	0.5541999356784338	0.5488441406500969
7	0.42740619621589837	0.4938216936950556
8	0.35064252319258693	0.4630052126859916
9	0.29212408235718273	0.43395148742192075
>10	3.631000635353574	14.51905007403348
>50	1.4577559711083927	17.40500020089778
>100	1.60431495379009	45.696380649633696
>500	0.005558214183747065	0.5834213495112187
>1k	0.0013474458627265613	0.34314160473729977
>5k	1.6843073284082016E-4	0.1480098763364971
>10k+	1.6843073284082016E-4	0.9560314871028924
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34389	0.9388508829932277	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5324	0.14535002765581856	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024434311561224196	0.0
2	0.0	0.0	0.0	0.08668037900210818	0.0
3	0.0	0.0	0.0	0.12555687024588835	0.0
4	0.0	0.0	0.0	0.18886767305089272	0.0
5	0.0	0.0	0.0	0.33028637013149753	0.0
6	0.0	0.0	0.0	0.46834705567910734	0.0
7	0.0	0.0	0.0	0.563408813060317	0.0
8	0.0	0.0	0.0	0.8247057917781682	0.0
9	0.0	0.0	0.0	0.937158226773344	0.0
10	0.0	0.0	0.0	1.139294140515583	0.0
11	0.0	0.0	0.0	1.3126002967062549	0.0
12	0.0	0.0	0.0	1.4836404776348242	0.0
13	0.0	0.0	0.0	1.5464052623043822	0.0
14	0.0	0.0	0.0	1.5696383339676243	0.0
15	2.7300906772317534E-5	0.0	0.0	1.6078323025420966	0.0
16	2.7300906772317534E-5	0.0	0.0	1.6951406023999682	0.0
17	2.7300906772317534E-5	0.0	0.0	1.813189723283469	0.0
18	2.7300906772317534E-5	0.0	0.0	1.968804891885679	0.0
19	2.7300906772317534E-5	0.0	0.0	2.048660044194708	0.0
20	2.7300906772317534E-5	0.0	0.0	2.143503394321739	0.0
21	2.7300906772317534E-5	0.0	0.0	2.261279506137517	0.0
22	2.7300906772317534E-5	0.0	0.0	2.3973472254907473	0.0
23	2.7300906772317534E-5	0.0	0.0	2.5372370717921027	0.0
24	2.7300906772317534E-5	0.0	0.0	2.6464133979746003	0.0
25	2.7300906772317534E-5	0.0	0.0	2.7394821891614307	0.0
26	2.7300906772317534E-5	0.0	0.0	2.8305034123403376	0.0
27	2.7300906772317534E-5	0.0	0.0	2.9226712736036813	0.0
28	2.7300906772317534E-5	0.0	0.0	3.0283257828125505	0.0
29	2.7300906772317534E-5	0.0	0.0	3.1436994148323643	0.0
30	2.7300906772317534E-5	0.0	0.0	3.2864558563448125	0.0
31	2.7300906772317534E-5	0.0	0.0	3.420093794995307	0.0
32	2.7300906772317534E-5	0.0	0.0	3.545514160707334	0.0
33	2.7300906772317534E-5	0.0	0.0	3.6721357663173424	0.0
34	2.7300906772317534E-5	0.0	0.0	3.8091044155940597	0.0
35	2.7300906772317534E-5	0.0	0.0	3.9653202041452604	0.0
36	2.7300906772317534E-5	0.0	0.0	4.112035277139695	0.0
37	5.460181354463507E-5	0.0	0.0	4.259405571896665	0.0
38	5.460181354463507E-5	0.0	0.0	4.41119861355075	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	25	4.444753E-5	44.0	31
CGTTTTT	23520	0.0	42.65306	1
CACGACC	275	0.0	40.8	27
CGACGGT	370	0.0	39.24324	28
TACGGGA	2200	0.0	39.2	4
CCTTACG	45	2.3534085E-8	39.11111	38
GGCGATA	1340	0.0	39.074627	8
AGGGCGA	3120	0.0	39.064102	6
GGGCGAT	5625	0.0	38.289776	7
AGGGATC	6960	0.0	38.02586	6
CTCGCGA	35	7.2945677E-6	37.714287	25
CGTAAGG	415	0.0	37.638554	2
AATACGG	415	0.0	37.638554	2
TCGTAAG	240	0.0	37.583332	1
CGAGGGA	3465	0.0	37.460316	4
TAGGGAT	9240	0.0	37.333332	5
GCGATAA	460	0.0	37.304348	9
ATGGGAC	4205	0.0	37.30321	5
TGTTGCG	295	0.0	37.288136	1
TAGGGAC	4945	0.0	37.2821	5
>>END_MODULE