FastQCFastQC Report
Sat 18 Jun 2016
SRR3549126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328493
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154701.1644773438776117No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29560.22250775879135232No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA19840.14934214933763293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA207.856924E-444.00000411
CGAACGT207.856924E-444.00000437
TCCTCGA207.856924E-444.00000444
ATATCGC207.856924E-444.00000441
CGGTCGT207.856924E-444.00000421
CGGTCGA207.856924E-444.00000420
CGCGTCA207.856924E-444.00000439
CCACGAA207.856924E-444.00000411
TACCGCG207.856924E-444.00000435
AATCCGT302.528017E-644.043
ATCTCGC700.044.010
GATCGAC351.4463149E-744.035
TAGAACG302.528017E-644.01
TTCGTAC254.443217E-544.032
CACGAAC302.528017E-644.041
TAACGCG302.528017E-644.01
GACCCGT254.443217E-544.025
CCCGTCG254.443217E-544.027
TACGACG254.443217E-544.01
CGGGCTA302.528017E-644.06
Produced by FastQC (version 0.11.5)