##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549126_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1328493
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.591680197035288	31.0	31.0	33.0	30.0	34.0
2	32.00993080129139	31.0	31.0	34.0	30.0	34.0
3	32.037845137309716	33.0	31.0	34.0	30.0	34.0
4	35.80341334128219	37.0	35.0	37.0	35.0	37.0
5	35.749004322943364	37.0	35.0	37.0	33.0	37.0
6	35.799192016819056	37.0	35.0	37.0	35.0	37.0
7	36.03623203133174	37.0	35.0	37.0	35.0	37.0
8	36.0213994353	37.0	35.0	37.0	35.0	37.0
9	37.81435957886116	39.0	38.0	39.0	35.0	39.0
10	37.37137342838841	39.0	37.0	39.0	34.0	39.0
11	37.081811496184024	39.0	37.0	39.0	33.0	39.0
12	36.32812517642171	38.0	35.0	39.0	33.0	39.0
13	35.9934881102121	38.0	35.0	39.0	32.0	39.0
14	37.03229674525948	39.0	35.0	41.0	32.0	41.0
15	37.21394166171745	39.0	35.0	41.0	32.0	41.0
16	37.37879386643362	39.0	35.0	41.0	33.0	41.0
17	37.29677009965427	39.0	35.0	41.0	33.0	41.0
18	37.17817256093935	39.0	35.0	41.0	32.0	41.0
19	37.037593724618795	38.0	35.0	41.0	32.0	41.0
20	36.80809533810114	38.0	35.0	41.0	32.0	41.0
21	36.664622244904564	38.0	35.0	41.0	32.0	41.0
22	36.56939253725838	38.0	35.0	40.0	32.0	41.0
23	36.508489694714235	37.0	35.0	40.0	32.0	41.0
24	36.532204535515056	38.0	35.0	40.0	32.0	41.0
25	36.499172370498	37.0	35.0	40.0	32.0	41.0
26	36.264846709768136	37.0	35.0	40.0	31.0	41.0
27	36.2471635153516	37.0	35.0	40.0	31.0	41.0
28	36.24437689923846	37.0	35.0	40.0	31.0	41.0
29	36.23002003021469	37.0	35.0	40.0	31.0	41.0
30	36.10314243281673	37.0	35.0	40.0	31.0	41.0
31	35.87954095354661	37.0	35.0	40.0	30.0	41.0
32	35.59912472252394	37.0	35.0	40.0	30.0	41.0
33	35.28841552044309	37.0	34.0	40.0	28.0	41.0
34	34.97210674049468	37.0	34.0	40.0	25.0	41.0
35	34.7176763445498	37.0	34.0	40.0	24.0	41.0
36	34.52443708773776	37.0	34.0	40.0	23.0	41.0
37	34.4236853336826	36.0	34.0	40.0	23.0	41.0
38	34.34855810305361	36.0	34.0	40.0	23.0	41.0
39	34.2728204062799	36.0	34.0	40.0	23.0	41.0
40	34.154911617900886	36.0	34.0	40.0	23.0	41.0
41	34.09405393931319	36.0	34.0	40.0	22.0	41.0
42	34.06540041987425	36.0	33.0	40.0	22.0	41.0
43	33.94956390436381	36.0	33.0	40.0	21.0	41.0
44	33.81528543996845	35.0	33.0	40.0	21.0	41.0
45	33.71212945796478	35.0	33.0	40.0	20.0	41.0
46	33.67909729294772	35.0	33.0	40.0	20.0	41.0
47	33.638719210413605	35.0	33.0	40.0	20.0	41.0
48	33.55469543309599	35.0	33.0	40.0	20.0	41.0
49	33.516899223405765	35.0	33.0	40.0	20.0	41.0
50	33.36342457205269	35.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	6.0
13	8.0
14	14.0
15	44.0
16	90.0
17	212.0
18	563.0
19	1012.0
20	1901.0
21	3119.0
22	4793.0
23	7139.0
24	10724.0
25	16748.0
26	25698.0
27	32568.0
28	33487.0
29	32497.0
30	34392.0
31	38661.0
32	47136.0
33	61983.0
34	115895.0
35	197166.0
36	83464.0
37	104345.0
38	165994.0
39	308828.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.348151627445535	21.983631076716247	28.661648951104745	18.006568344733466
2	34.87447807402824	24.252442429128344	28.575084701236662	12.297994795606751
3	27.051026990733106	23.50482840331112	35.898871879641064	13.545272726314703
4	25.714625519291406	25.83024524781087	34.20567515222135	14.249454080676374
5	22.740578986867074	31.299675647519408	32.05812902288533	13.90161634272819
6	21.4719987233655	38.57302974121806	29.556949114522997	10.398022420893449
7	83.09257180880893	4.124071410237013	11.193886606854534	1.589470174099525
8	84.39931561551322	3.145594293684649	10.764829020551858	1.6902610702502758
9	79.56315915853527	5.619374735132214	12.165965496242736	2.651500610089778
10	45.02229217617255	29.255028065635276	16.33828706662361	9.384392691568568
11	42.56635149752388	18.94296770852387	25.645449392657692	12.84523140129455
12	38.69557460972696	19.44127669472101	27.814523674569607	14.048625020982422
13	21.537787553265243	34.92205077482531	28.291455054712372	15.24870661719708
14	15.892895182737131	36.51242422805389	32.179921158786684	15.41475943042229
15	15.641633038337424	21.802448338079312	47.19317301634258	15.362745607240685
16	18.049097737059963	18.69727578542002	45.075886737830004	18.17773973969001
17	17.700356719982718	18.618615227931198	32.89900661877782	30.782021433308266
18	22.03587071968012	21.745918119252416	36.017427265329964	20.2007838957375
19	26.63837897527499	23.78341474136484	29.22875769763183	20.349448585728343
20	28.97847410562193	22.157512309059964	30.124735320396873	18.73927826492123
21	21.825256136088033	25.78681257635531	31.731819437513032	20.656111850043622
22	22.889845862943954	22.35231950789353	30.936933803941756	23.820900825220757
23	19.457761538826325	27.09227673762677	29.699667216914204	23.750294506632702
24	19.81576116697642	22.55013763715729	39.7704767733063	17.863624422559997
25	17.426964236921084	24.643637565271327	37.39884214670307	20.530556051104522
26	17.387295228503273	30.877844294249197	31.18217408748108	20.55268638976645
27	18.464530863166008	29.218144167865397	32.767127865935315	19.550197103033288
28	15.70697022867264	26.97552791019599	39.363097886101016	17.954403975030353
29	17.007014715169746	23.269825283234464	38.848002962755544	20.87515703884025
30	19.34161489748158	27.084975231333548	34.24617216650746	19.327237704677405
31	27.23093008393721	24.980937046713834	28.973656616933624	18.81447625241533
32	27.66570843805726	24.848757200828306	29.80851235196572	17.677022009148715
33	24.87480174904949	27.61068368444546	27.9655971089046	19.548917457600453
34	19.702700729322622	27.217757263305113	31.140773794065908	21.938768213306357
35	20.370901465043474	25.458997525767916	32.380223305655356	21.78987770353325
36	26.305144249913248	26.50469366417437	28.738502950335455	18.45165913557693
37	21.01531585036579	30.565535535377304	29.956123216305997	18.46302539795091
38	20.04978573466326	30.958461956517645	26.98794799822054	22.00380431059855
39	21.016369676016357	30.461206795971073	28.584117492527245	19.93830603548532
40	23.402080402380744	26.419634879521382	27.239511235663265	22.93877348243461
41	17.64939672245168	25.4586964327249	30.195266365724173	26.696640479099248
42	21.984835448888326	25.460804084026034	26.863521298192765	25.690839168892875
43	22.11852075998895	26.22625787264216	27.11628890780757	24.538932459561323
44	20.05023737422779	29.03297194640845	28.766429330075503	22.150361349288254
45	17.972695377393784	34.394309943673015	25.90198066531024	21.731014013622953
46	21.108579420441057	31.20099240266979	27.795554812859386	19.89487336402977
47	21.294429101244795	27.41226336909566	29.264136130186607	22.029171399472936
48	22.422022547352526	24.029859397076237	32.10818574128731	21.43993231428393
49	20.899395028803312	24.051989735738164	32.262947565399294	22.785667670059233
50	19.013122387547394	30.657368913498228	29.344829065715818	20.984679633238564
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	414.0
1	1308.5
2	2203.0
3	15711.0
4	29219.0
5	20775.5
6	12332.0
7	12148.5
8	11965.0
9	12075.5
10	12186.0
11	11778.5
12	11371.0
13	10694.5
14	10018.0
15	9177.0
16	8336.0
17	7532.5
18	6729.0
19	6661.5
20	6594.0
21	6216.0
22	5838.0
23	5907.0
24	5976.0
25	7199.5
26	8423.0
27	11092.0
28	13761.0
29	16001.5
30	18242.0
31	22171.5
32	26101.0
33	28433.5
34	30766.0
35	33619.0
36	36472.0
37	37974.5
38	39477.0
39	49397.5
40	59318.0
41	79181.0
42	99044.0
43	110258.5
44	121473.0
45	124277.0
46	127081.0
47	114977.0
48	102873.0
49	95560.0
50	88247.0
51	84690.5
52	81134.0
53	77401.0
54	73668.0
55	66365.0
56	59062.0
57	55231.5
58	51401.0
59	49627.0
60	47853.0
61	44137.5
62	40422.0
63	33657.5
64	26893.0
65	22957.0
66	19021.0
67	15479.0
68	11937.0
69	10261.5
70	8586.0
71	7626.0
72	6666.0
73	5104.0
74	3542.0
75	2616.5
76	1691.0
77	1344.0
78	997.0
79	737.0
80	477.0
81	439.5
82	402.0
83	278.0
84	154.0
85	111.0
86	68.0
87	49.5
88	31.0
89	21.5
90	12.0
91	10.5
92	9.0
93	6.5
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1328493.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.91971026670475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.78933702591233	14.477215121435115
2	6.642811890760959	2.380745288773151
3	2.2151767440943817	1.1908597633114109
4	1.0751657067574405	0.7706663181516071
5	0.6972104873799673	0.6246904964378511
6	0.5343474468684679	0.5745210857781814
7	0.4007049720425076	0.5026361901000834
8	0.3394330353622889	0.48660333189122923
9	0.2910864972534183	0.46945671239980835
>10	4.00672273550368	17.81449712452439
>50	1.5824898305973265	20.592294795372478
>100	1.4216952873936397	38.17456169405884
>500	0.0016970400326990628	0.20686719290361363
>1k	0.0016970400326990628	0.5582601240320599
>5k	0.0	0.0
>10k+	4.242600081747657E-4	1.176124760830174
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15470	1.1644773438776117	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2956	0.22250775879135232	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1984	0.14934214933763293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017915036059655564	0.0
2	0.0	0.0	0.0	0.07045577206654458	0.0
3	0.0	0.0	0.0	0.10207054158358381	0.0
4	0.0	0.0	0.0	0.17583833712334201	0.0
5	0.0	0.0	0.0	0.32886887623796285	0.0
6	0.0	0.0	0.0	0.4761786475352147	0.0
7	0.0	0.0	0.0	0.5793782880301214	0.0
8	0.0	0.0	0.0	0.8666210510706492	0.0
9	0.0	0.0	0.0	1.0109951651984617	0.0
10	0.0	0.0	0.0	1.2474284772294622	0.0
11	0.0	0.0	0.0	1.4201053374011003	0.0
12	0.0	0.0	0.0	1.5802115630266775	0.0
13	0.0	0.0	0.0	1.6436669218430207	0.0
14	0.0	0.0	0.0	1.6645928883328704	0.0
15	0.0	0.0	0.0	1.700648780234446	0.0
16	0.0	0.0	0.0	1.7822449948927093	0.0
17	0.0	0.0	0.0	1.8799496873525114	0.0
18	0.0	0.0	0.0	2.0313994879912802	0.0
19	0.0	0.0	0.0	2.096209765501211	0.0
20	0.0	0.0	0.0	2.1691495551726656	0.0
21	0.0	0.0	0.0	2.2643702300275574	0.0
22	0.0	0.0	0.0	2.3606447305330174	0.0
23	0.0	0.0	0.0	2.4645971036354726	0.0
24	0.0	0.0	0.0	2.5477740567695877	0.0
25	0.0	0.0	0.0	2.614691985580654	0.0
26	0.0	0.0	0.0	2.6825131935207787	0.0
27	0.0	0.0	0.0	2.744463087122025	0.0
28	0.0	0.0	0.0	2.8183814291832925	0.0
29	0.0	0.0	0.0	2.892224497983806	0.0
30	0.0	0.0	0.0	2.9933164871775766	0.0
31	0.0	0.0	0.0	3.0916233657234176	0.0
32	0.0	0.0	0.0	3.184134203191135	0.0
33	0.0	0.0	0.0	3.2716770054490314	0.0
34	0.0	0.0	0.0	3.366370767478639	0.0
35	0.0	0.0	0.0	3.4817646762158327	0.0
36	0.0	0.0	0.0	3.58172756649828	0.0
37	0.0	0.0	0.0	3.680184991565631	0.0
38	0.0	0.0	0.0	3.7836857251035574	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	20	7.856924E-4	44.000004	11
CGAACGT	20	7.856924E-4	44.000004	37
TCCTCGA	20	7.856924E-4	44.000004	44
ATATCGC	20	7.856924E-4	44.000004	41
CGGTCGT	20	7.856924E-4	44.000004	21
CGGTCGA	20	7.856924E-4	44.000004	20
CGCGTCA	20	7.856924E-4	44.000004	39
CCACGAA	20	7.856924E-4	44.000004	11
TACCGCG	20	7.856924E-4	44.000004	35
AATCCGT	30	2.528017E-6	44.0	43
ATCTCGC	70	0.0	44.0	10
GATCGAC	35	1.4463149E-7	44.0	35
TAGAACG	30	2.528017E-6	44.0	1
TTCGTAC	25	4.443217E-5	44.0	32
CACGAAC	30	2.528017E-6	44.0	41
TAACGCG	30	2.528017E-6	44.0	1
GACCCGT	25	4.443217E-5	44.0	25
CCCGTCG	25	4.443217E-5	44.0	27
TACGACG	25	4.443217E-5	44.0	1
CGGGCTA	30	2.528017E-6	44.0	6
>>END_MODULE