FastQCFastQC Report
Sat 18 Jun 2016
SRR3549121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650694
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93100.5640052002369912No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA76600.46404724315954377No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA31730.19222218048893375No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA23790.1441211999316651No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA22950.13903243120772232No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA18440.11171058960655336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG207.8574824E-444.01
GGTACTC502.7284841E-1144.08
GATACGT351.4466059E-744.043
GCCCATA254.443686E-544.011
ATTAGTC207.8574824E-444.037
GGTCGAC502.7284841E-1144.09
ACGTTGC408.3164196E-944.017
ACCATCG254.443686E-544.013
TTAGCCG454.802132E-1044.016
CTATACG254.443686E-544.01
CGTTGCC408.3164196E-944.018
GCTATGC351.4466059E-744.09
TTCGTCA254.443686E-544.043
CGACACT207.8574824E-444.036
TATGCGC207.8574824E-444.038
CCGTCGA254.443686E-544.014
TTTCACG254.443686E-544.01
ACGATTC254.443686E-544.07
ATATCGA351.4466059E-744.018
CCGTAAG302.5283916E-644.01
Produced by FastQC (version 0.11.5)