##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549121_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1650694
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.736716799116007	31.0	31.0	33.0	30.0	34.0
2	32.16280425081814	33.0	31.0	34.0	30.0	34.0
3	32.20997410786009	34.0	31.0	34.0	30.0	34.0
4	35.8985572129056	37.0	35.0	37.0	35.0	37.0
5	35.88578440340851	37.0	35.0	37.0	35.0	37.0
6	35.941671200113404	37.0	35.0	37.0	35.0	37.0
7	36.1084107654114	37.0	35.0	37.0	35.0	37.0
8	36.04855594071342	37.0	35.0	37.0	35.0	37.0
9	37.78994834899745	39.0	38.0	39.0	35.0	39.0
10	37.59541017293332	39.0	37.0	39.0	35.0	39.0
11	37.34289698757008	39.0	37.0	39.0	35.0	39.0
12	36.4743144398659	38.0	35.0	39.0	33.0	39.0
13	36.18621258694828	38.0	35.0	39.0	33.0	39.0
14	37.15252251477258	39.0	35.0	41.0	33.0	41.0
15	37.34768588242279	39.0	35.0	41.0	33.0	41.0
16	37.449311017063124	39.0	35.0	41.0	33.0	41.0
17	37.39269361856286	39.0	35.0	41.0	33.0	41.0
18	37.32144176934065	39.0	35.0	41.0	33.0	41.0
19	37.24352847953649	38.0	35.0	41.0	33.0	41.0
20	37.08390531497661	38.0	35.0	41.0	33.0	41.0
21	36.943687927623166	38.0	35.0	41.0	33.0	41.0
22	36.949567272916724	38.0	35.0	41.0	33.0	41.0
23	36.94698108795452	38.0	35.0	41.0	33.0	41.0
24	36.92272462370373	38.0	35.0	41.0	33.0	41.0
25	36.84010058799511	38.0	35.0	41.0	33.0	41.0
26	36.72306314798503	38.0	35.0	41.0	33.0	41.0
27	36.63589072232649	38.0	35.0	41.0	32.0	41.0
28	36.59723122516954	38.0	35.0	41.0	32.0	41.0
29	36.58967379780868	38.0	35.0	41.0	32.0	41.0
30	36.507773094225826	38.0	35.0	41.0	32.0	41.0
31	36.34200402982018	38.0	35.0	41.0	31.0	41.0
32	36.14465491484188	38.0	35.0	41.0	31.0	41.0
33	35.987386517428426	38.0	35.0	41.0	30.0	41.0
34	35.77649764280963	37.0	35.0	40.0	30.0	41.0
35	35.58139970218587	37.0	35.0	40.0	29.0	41.0
36	35.40790358479524	37.0	35.0	40.0	29.0	41.0
37	35.32063847084923	37.0	35.0	40.0	28.0	41.0
38	35.2740114158045	37.0	35.0	40.0	28.0	41.0
39	35.22193089694395	37.0	35.0	40.0	27.0	41.0
40	35.05396578651161	37.0	34.0	40.0	27.0	41.0
41	35.01034231662561	36.0	34.0	40.0	27.0	41.0
42	34.95855319035509	36.0	34.0	40.0	27.0	41.0
43	34.87161702895873	36.0	34.0	40.0	26.0	41.0
44	34.72677552593031	36.0	34.0	40.0	26.0	41.0
45	34.634518572188426	36.0	34.0	40.0	25.0	41.0
46	34.59717306781269	35.0	34.0	40.0	26.0	41.0
47	34.546267812205045	35.0	34.0	40.0	25.0	41.0
48	34.486237303824936	35.0	34.0	40.0	25.0	41.0
49	34.41703610723732	36.0	34.0	40.0	24.0	41.0
50	34.26315234683109	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	2.0
12	6.0
13	12.0
14	12.0
15	37.0
16	81.0
17	199.0
18	497.0
19	990.0
20	1800.0
21	3031.0
22	4761.0
23	7245.0
24	10562.0
25	16000.0
26	22917.0
27	28554.0
28	30469.0
29	30900.0
30	34736.0
31	40752.0
32	51542.0
33	68508.0
34	142571.0
35	296861.0
36	87981.0
37	116504.0
38	198688.0
39	454471.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.42375994581673	23.457042916494515	28.595124232595502	14.524072905093252
2	32.31507475037772	25.741960654124874	29.184209792971927	12.758754802525482
3	29.414294835990194	23.989061570466724	33.006299168713284	13.590344424829798
4	26.543381147565814	26.902563406664104	31.12914931537886	15.424906130391216
5	23.446017250925973	32.283088204112936	30.367833165929	13.903061379032092
6	21.783443812117813	41.77121865106434	26.596510316266976	9.84882722055087
7	85.0543468383601	5.146380855567416	8.026623953319028	1.772648352753448
8	86.7696253818091	3.9794171421232525	7.407793328139559	1.8431641479280836
9	81.84660512487476	5.953314181792628	9.003182903675667	3.1968977896569566
10	50.74623158501817	25.192373631939052	13.974849366387712	10.086545416655056
11	46.26805452736849	19.798884590360174	20.972815070509736	12.960245811761597
12	42.50751502095482	20.45479053052837	24.138937925502848	12.898756523013958
13	22.30158951325927	39.30486207619341	23.937628658006872	14.455919752540447
14	15.128606513381646	40.54706687005587	29.61263565506387	14.711690961498618
15	14.842363272659863	23.008019657186612	48.23983124673622	13.909785823417304
16	16.77918499734051	17.931548790993364	45.63868288126085	19.650583330405272
17	16.07475401255472	18.78609845313547	30.664435685838804	34.47471184847101
18	21.672944834112197	22.28771656042852	35.52554258996519	20.51379601549409
19	27.813028944189533	23.584867940393554	27.64849208878205	20.953611026634857
20	29.88761090789692	22.063507833674805	27.699198034281338	20.349683224146933
21	21.86692385142249	26.886327811211526	30.223651385417284	21.023096951948695
22	22.885646885491802	24.181526073275847	26.948665228079825	25.98416181315253
23	19.170966878173665	29.326089511441854	26.70725161659278	24.795691993791703
24	19.720977964419813	23.515200273339577	39.0744741302749	17.689347631965706
25	17.40637574256646	25.24283725511815	35.979896940317225	21.370890061998164
26	17.14424357270336	33.030349658991916	29.551570430376557	20.27383633792817
27	18.460356674223085	32.162048205179154	30.187787682029498	19.189807438568263
28	14.520014006230106	28.45645528486806	39.047758094474204	17.97577261442763
29	16.751620833419157	24.151659847312708	37.789257124579116	21.307462194689023
30	17.72987604001711	29.36152915076931	33.15769003825058	19.750904770963
31	27.42161781650627	26.471835482530377	27.058679561445064	19.04786713951829
32	27.701076032262794	27.415741500241715	28.47965764702604	16.40352482046945
33	26.57979007617402	28.205288199993454	26.34170839658956	18.873213327242965
34	19.388148257641937	27.3191760556469	30.338693906926423	22.953981779784748
35	21.72789142021477	25.249016474282936	31.272180064869687	21.750912040632606
36	28.390361872036852	25.97192453598305	27.171662343232605	18.466051248747497
37	20.714257154869404	31.806197877983443	29.92856338000865	17.5509815871385
38	21.00140910429189	31.767244565013257	25.40264882528197	21.828697505412876
39	21.40166499666201	30.2738726862762	28.519277346376736	19.80518497068506
40	22.808709548832187	27.222065385831655	26.598751797728713	23.37047326760744
41	17.679594158578148	26.060432763431624	28.081703816697708	28.17826926129252
42	21.805313401514756	26.30796501350341	25.87796405633025	26.008757528651582
43	22.379496139199635	26.907652175388048	26.52005762424774	24.192794061164577
44	19.63259089813133	31.550487249605318	27.997012165792086	20.819909686471266
45	17.548376622196482	36.52106326187652	24.618433216574363	21.312126899352634
46	21.615393283067608	32.74755951133281	26.989738861351647	18.647308344247936
47	22.611580341359453	27.783950265767004	28.035056770061562	21.56941262281198
48	23.139600677048563	24.52386693112109	31.132481247281447	21.2040511445489
49	20.765690067329256	25.27446031790265	32.70260872093798	21.257240893830108
50	18.773679434225844	32.27472808406646	29.217105047937412	19.73448743377028
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	889.0
1	2289.5
2	3690.0
3	11272.5
4	18855.0
5	14040.0
6	9225.0
7	8904.5
8	8584.0
9	8816.0
10	9048.0
11	8574.5
12	8101.0
13	7711.5
14	7322.0
15	7009.0
16	6696.0
17	6777.0
18	6858.0
19	5954.0
20	5050.0
21	5370.0
22	5690.0
23	6100.0
24	6510.0
25	8455.5
26	10401.0
27	12917.5
28	15434.0
29	20110.0
30	24786.0
31	30207.0
32	35628.0
33	38314.0
34	41000.0
35	40980.5
36	40961.0
37	44520.5
38	48080.0
39	61716.0
40	75352.0
41	105183.0
42	135014.0
43	153985.5
44	172957.0
45	174359.0
46	175761.0
47	162846.5
48	149932.0
49	142218.0
50	134504.0
51	125581.0
52	116658.0
53	107041.0
54	97424.0
55	87048.5
56	76673.0
57	68930.0
58	61187.0
59	56784.0
60	52381.0
61	43177.5
62	33974.0
63	28262.5
64	22551.0
65	18423.5
66	14296.0
67	10947.0
68	7598.0
69	6170.0
70	4742.0
71	3927.5
72	3113.0
73	2347.0
74	1581.0
75	1297.5
76	1014.0
77	809.0
78	604.0
79	469.5
80	335.0
81	219.0
82	103.0
83	85.5
84	68.0
85	49.5
86	31.0
87	22.5
88	14.0
89	12.0
90	10.0
91	7.5
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1650694.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.519685583322573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.30522596764887	11.80526317331255
2	7.0911761117216185	2.059232951163316
3	2.2801886692801254	0.9932286764580636
4	1.0957878618458574	0.6364198088009264
5	0.6843895802121087	0.4968560760590971
6	0.4511067350136702	0.3929956774150622
7	0.3519883029794442	0.3577531641788169
8	0.26862124577134483	0.3120236823680276
9	0.21814808436307953	0.28506974360004383
>10	3.131643595207181	10.983414575242437
>50	1.0806291487724589	11.325980044868649
>100	2.0238080038182864	57.334774250097574
>500	0.013913680026250718	1.2307440911297816
>1k	0.0025297600047728577	0.7473599216331265
>5k	8.43253334924286E-4	1.0388841636725226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9310	0.5640052002369912	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	7660	0.46404724315954377	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3173	0.19222218048893375	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2379	0.1441211999316651	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2295	0.13903243120772232	No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	1844	0.11171058960655336	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024656296079103697	0.0
2	0.0	0.0	0.0	0.10843923828401872	0.0
3	0.0	0.0	0.0	0.14454526399199366	0.0
4	0.0	0.0	0.0	0.2043382964983213	0.0
5	0.0	0.0	0.0	0.3545781350147271	0.0
6	0.0	0.0	0.0	0.45114357960954604	0.0
7	0.0	0.0	0.0	0.5345630383341794	0.0
8	0.0	0.0	0.0	0.7314499234867274	0.0
9	0.0	0.0	0.0	0.8383746472695727	0.0
10	0.0	0.0	0.0	1.1290402703347804	0.0
11	0.0	0.0	0.0	1.3298648931903794	0.0
12	0.0	0.0	0.0	1.5615856118699165	0.0
13	0.0	0.0	0.0	1.6365843699680256	0.0
14	0.0	0.0	0.0	1.6609377631468947	0.0
15	0.0	0.0	0.0	1.7080088738433652	0.0
16	0.0	0.0	0.0	1.8005154195750392	0.0
17	0.0	0.0	0.0	1.9058650482766641	0.0
18	0.0	0.0	0.0	2.0819122138930655	0.0
19	0.0	0.0	0.0	2.145037178301975	0.0
20	0.0	0.0	0.0	2.21694632681769	0.0
21	0.0	0.0	0.0	2.305757457166501	0.0
22	0.0	0.0	0.0	2.400990129000287	0.0
23	0.0	0.0	0.0	2.4981613793955755	0.0
24	0.0	0.0	0.0	2.5750987160551864	0.0
25	0.0	0.0	0.0	2.6345888456612796	0.0
26	0.0	0.0	0.0	2.6989254216711274	0.0
27	0.0	0.0	0.0	2.760899355059145	0.0
28	0.0	0.0	0.0	2.8217222574262704	0.0
29	0.0	0.0	0.0	2.89314676130161	0.0
30	0.0	0.0	0.0	2.9814126664299985	0.0
31	0.0	0.0	0.0	3.069678571558387	0.0
32	0.0	0.0	0.0	3.148736228519641	0.0
33	0.0	0.0	0.0	3.2269457573602374	0.0
34	0.0	0.0	0.0	3.308426637523369	0.0
35	0.0	0.0	0.0	3.4144426526055103	0.0
36	6.0580580046937835E-5	0.0	0.0	3.50149694613296	0.0
37	6.0580580046937835E-5	0.0	0.0	3.594064072444681	0.0
38	6.0580580046937835E-5	0.0	0.0	3.6813606882923184	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	20	7.8574824E-4	44.0	1
GGTACTC	50	2.7284841E-11	44.0	8
GATACGT	35	1.4466059E-7	44.0	43
GCCCATA	25	4.443686E-5	44.0	11
ATTAGTC	20	7.8574824E-4	44.0	37
GGTCGAC	50	2.7284841E-11	44.0	9
ACGTTGC	40	8.3164196E-9	44.0	17
ACCATCG	25	4.443686E-5	44.0	13
TTAGCCG	45	4.802132E-10	44.0	16
CTATACG	25	4.443686E-5	44.0	1
CGTTGCC	40	8.3164196E-9	44.0	18
GCTATGC	35	1.4466059E-7	44.0	9
TTCGTCA	25	4.443686E-5	44.0	43
CGACACT	20	7.8574824E-4	44.0	36
TATGCGC	20	7.8574824E-4	44.0	38
CCGTCGA	25	4.443686E-5	44.0	14
TTTCACG	25	4.443686E-5	44.0	1
ACGATTC	25	4.443686E-5	44.0	7
ATATCGA	35	1.4466059E-7	44.0	18
CCGTAAG	30	2.5283916E-6	44.0	1
>>END_MODULE