##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549119_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	715937
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71366474983134	31.0	31.0	33.0	30.0	34.0
2	32.13845771345803	33.0	31.0	34.0	30.0	34.0
3	32.192269710882385	33.0	31.0	34.0	30.0	34.0
4	35.89276570424493	37.0	35.0	37.0	35.0	37.0
5	35.86505516546847	37.0	35.0	37.0	35.0	37.0
6	35.92581609834385	37.0	35.0	37.0	35.0	37.0
7	36.07369642859637	37.0	35.0	37.0	35.0	37.0
8	36.00077381110349	37.0	35.0	37.0	35.0	37.0
9	37.76303780919271	39.0	38.0	39.0	35.0	39.0
10	37.58018792156293	39.0	37.0	39.0	35.0	39.0
11	37.34330674347044	39.0	37.0	39.0	35.0	39.0
12	36.4628451944794	38.0	35.0	39.0	33.0	39.0
13	36.17650016691413	38.0	35.0	39.0	33.0	39.0
14	37.129976520280415	39.0	35.0	41.0	33.0	41.0
15	37.346360084756064	39.0	35.0	41.0	33.0	41.0
16	37.441252512441736	39.0	35.0	41.0	33.0	41.0
17	37.39606697237327	39.0	35.0	41.0	33.0	41.0
18	37.332128385598175	39.0	35.0	41.0	33.0	41.0
19	37.253993018938814	38.0	35.0	41.0	33.0	41.0
20	37.083265706340086	38.0	35.0	41.0	33.0	41.0
21	36.94849127786384	38.0	35.0	41.0	33.0	41.0
22	36.93697490142289	38.0	35.0	41.0	33.0	41.0
23	36.935992971448606	38.0	35.0	41.0	33.0	41.0
24	36.89067194459848	38.0	35.0	41.0	33.0	41.0
25	36.79603652276667	38.0	35.0	41.0	33.0	41.0
26	36.66818588786443	38.0	35.0	41.0	32.0	41.0
27	36.57078346279072	38.0	35.0	41.0	32.0	41.0
28	36.54632879708689	38.0	35.0	41.0	32.0	41.0
29	36.526137076306995	38.0	35.0	41.0	32.0	41.0
30	36.45974715652355	38.0	35.0	41.0	32.0	41.0
31	36.2935830946019	38.0	35.0	41.0	31.0	41.0
32	36.079796127312875	38.0	35.0	41.0	31.0	41.0
33	35.927224043456334	38.0	35.0	41.0	30.0	41.0
34	35.79157244282667	37.0	35.0	40.0	30.0	41.0
35	35.56937272413634	37.0	35.0	40.0	29.0	41.0
36	35.42505276302244	37.0	35.0	40.0	29.0	41.0
37	35.32111065638457	37.0	35.0	40.0	28.0	41.0
38	35.25270100581476	37.0	34.0	40.0	28.0	41.0
39	35.21635981936958	37.0	35.0	40.0	27.0	41.0
40	35.07585304293534	37.0	34.0	40.0	27.0	41.0
41	35.0009358365331	36.0	34.0	40.0	26.0	41.0
42	35.00069419515963	36.0	34.0	40.0	27.0	41.0
43	34.912238087988186	36.0	34.0	40.0	27.0	41.0
44	34.8045121288605	36.0	34.0	40.0	26.0	41.0
45	34.691706113806106	36.0	34.0	40.0	26.0	41.0
46	34.6766405423941	36.0	34.0	40.0	26.0	41.0
47	34.63384627418334	35.0	34.0	40.0	26.0	41.0
48	34.58406815124795	35.0	34.0	40.0	26.0	41.0
49	34.532940747579744	36.0	34.0	40.0	26.0	41.0
50	34.37304399688799	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	4.0
13	4.0
14	7.0
15	18.0
16	47.0
17	102.0
18	254.0
19	483.0
20	889.0
21	1406.0
22	2192.0
23	3126.0
24	4697.0
25	7046.0
26	9696.0
27	12061.0
28	13083.0
29	13434.0
30	14994.0
31	17491.0
32	22126.0
33	29438.0
34	61503.0
35	130081.0
36	38364.0
37	50148.0
38	85709.0
39	197533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.177448574385735	23.010963255146752	28.50530144412148	14.306286726346032
2	32.68248463202768	25.510065829814632	28.94696041690819	12.860489121249497
3	29.220029136641912	25.696534750962723	32.006447494681794	13.07698861771357
4	27.119704666751403	26.86828589666409	31.382649590676277	14.62935984590823
5	23.25889009787174	32.28356685015581	30.477681695456443	13.979861356516007
6	20.45557081139821	41.145659464450084	27.55116721164013	10.847602512511575
7	84.86305359270439	5.085363656299367	7.985758523445499	2.065824227550748
8	86.15031769555142	4.133464257329904	7.874994587512589	1.8412234596060826
9	81.64503301268128	5.748410823857406	9.138932615579304	3.4676235478820066
10	49.86919240100735	25.205150732536524	13.994806805626753	10.930850060829375
11	46.56974007489485	19.91516013280498	21.262485386283988	12.252614406016171
12	42.50695242737839	20.73660112551803	24.537354543765723	12.219091903337864
13	22.146501717329876	39.4016512626111	24.588197006161156	13.863650013897871
14	14.769316294590165	42.34059700783728	27.694056879306416	15.196029818266133
15	15.203153350085271	23.24799528450129	47.039334466580165	14.509516898833278
16	16.300875635705374	18.13776910538218	46.92591666585189	18.635438593060563
17	16.264838945326197	18.95376269141	30.496817457401974	34.28458090586183
18	21.82286988938971	22.826030782038085	34.05676756474382	21.29433176382838
19	27.83024204643705	23.501788565194982	27.468478371700307	21.199491016667668
20	30.705774390763434	22.02651909316043	26.89286906529485	20.374837450781282
21	22.35769348420322	27.04763128599304	29.15731412121458	21.437361108589165
22	21.93656704430697	24.823971941665256	27.842254276563438	25.39720673746433
23	20.002178962674087	28.48784180730986	27.097356331632533	24.412622898383518
24	19.16830670855117	23.49606180432077	39.11251967701069	18.22311181011737
25	17.529056327581895	25.772239736177905	36.10904311412876	20.589660822111444
26	16.84016889754266	33.94530524333845	28.788287237564198	20.426238621554692
27	18.9082279586053	32.09472341840134	29.489745606107796	19.50730301688556
28	15.623860758698042	27.733166465764448	39.137661554019424	17.505311221518095
29	16.22056130637193	24.93096459604686	37.26445203977445	21.584022057806763
30	17.730750052029716	30.08295422641936	32.75232317927416	19.433972542276763
31	28.57583837684042	26.6759505375473	26.33667487502392	18.411536210588363
32	28.830050688817593	26.51490284759693	27.506051510118905	17.148994953466577
33	25.99362793094923	28.45781123199388	26.358324824670326	19.190236012386563
34	19.76696273554796	28.067134398697092	29.229247824878446	22.936655040876502
35	21.277011804111257	26.140707911450313	31.507381236058478	21.074899048379955
36	28.891229256205502	26.70681917543024	26.046844903951044	18.35510666441321
37	20.83800669611991	32.70832489450887	28.601119930943646	17.852548478427572
38	20.576112143945625	33.05640021398531	25.242304839671647	21.12518280239742
39	21.22030290374712	31.438799782662443	27.482306404055105	19.858590909535337
40	24.308563462986267	27.442638109219107	26.178141372774423	22.070657055020206
41	17.789693785905744	26.908512899878062	27.627989613611252	27.673803700604942
42	21.59240268347634	27.6390241040762	24.815172284712204	25.95340092773526
43	23.101893043661665	27.23759213450345	25.989996326492417	23.670518495342467
44	20.355422334646764	30.501566478614738	28.00204487266338	21.140966314075122
45	16.89450328730042	37.229393089056714	23.48418925128887	22.39191437235399
46	20.97991862412475	34.03246375030205	26.216133542476506	18.7714840830967
47	22.198880627764733	27.76836509357667	27.877592581470157	22.15516169718844
48	23.201901843318616	24.878166654328524	30.818074774735766	21.101856727617093
49	20.835352831324546	25.804086113722295	32.41695847539658	20.94360257955658
50	20.154426995671407	32.209537990074544	28.02020289494746	19.615832119306585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	505.0
1	1035.5
2	1566.0
3	5070.5
4	8575.0
5	6300.0
6	4025.0
7	3823.5
8	3622.0
9	3695.5
10	3769.0
11	3641.0
12	3513.0
13	3236.0
14	2959.0
15	2764.0
16	2569.0
17	2331.5
18	2094.0
19	2164.5
20	2235.0
21	2384.5
22	2534.0
23	2916.5
24	3299.0
25	3448.5
26	3598.0
27	5897.0
28	8196.0
29	9541.5
30	10887.0
31	12525.5
32	14164.0
33	15912.5
34	17661.0
35	19382.0
36	21103.0
37	20563.5
38	20024.0
39	26465.5
40	32907.0
41	45415.5
42	57924.0
43	66017.0
44	74110.0
45	74868.0
46	75626.0
47	69632.0
48	63638.0
49	60556.5
50	57475.0
51	53728.0
52	49981.0
53	47853.0
54	45725.0
55	39736.0
56	33747.0
57	30155.5
58	26564.0
59	24375.0
60	22186.0
61	18628.0
62	15070.0
63	12253.5
64	9437.0
65	7523.0
66	5609.0
67	4587.5
68	3566.0
69	2853.0
70	2140.0
71	1767.0
72	1394.0
73	1174.5
74	955.0
75	713.0
76	471.0
77	367.5
78	264.0
79	186.5
80	109.0
81	88.5
82	68.0
83	49.0
84	30.0
85	21.5
86	13.0
87	15.0
88	17.0
89	11.5
90	6.0
91	5.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	715937.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.720980057494902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.19674137320598	13.242471331199587
2	5.57628212579568	1.8648180444078966
3	1.9543564808395169	0.9803626722406044
4	1.1914655721035505	0.7968988828134089
5	0.8470986568351324	0.7082159873835483
6	0.6553296262724179	0.657465216719201
7	0.5418803304442776	0.634253913923526
8	0.4788662693260188	0.6405690671685874
9	0.40157478600859836	0.60432515895983
>10	5.566596775800019	21.93128937397293
>50	2.3254361229405087	28.245795805836867
>100	1.2584910809844227	28.126672726938406
>500	0.0025203426187939038	0.24962465943698114
>1k	0.003360456825058539	1.3172371589986338
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4362	0.6092714861782531	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1995	0.27865580351343766	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1743	0.24345717570121392	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1277	0.17836764966749868	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019275439040027265	0.0
2	0.0	0.0	0.0	0.07388918298677118	0.0
3	0.0	0.0	0.0	0.10042783094043191	0.0
4	0.0	0.0	0.0	0.14721965759557057	0.0
5	0.0	0.0	0.0	0.28256676215924026	0.0
6	0.0	0.0	0.0	0.36218270602022246	0.0
7	0.0	0.0	0.0	0.42629449239248707	0.0
8	0.0	0.0	0.0	0.5693238371532691	0.0
9	0.0	0.0	0.0	0.6226804872495765	0.0
10	0.0	0.0	0.0	0.7855439794283575	0.0
11	0.0	0.0	0.0	0.878708601455156	0.0
12	0.0	0.0	0.0	0.9910089854274887	0.0
13	0.0	0.0	0.0	1.0296995406020362	0.0
14	0.0	0.0	0.0	1.041851447822923	0.0
15	0.0	0.0	0.0	1.066993324831654	0.0
16	0.0	0.0	0.0	1.1241212564792713	0.0
17	0.0	0.0	0.0	1.182087250693846	0.0
18	0.0	0.0	0.0	1.2805596023113766	0.0
19	0.0	0.0	0.0	1.319110480391431	0.0
20	0.0	0.0	0.0	1.3600358690778658	0.0
21	0.0	0.0	0.0	1.4118560711347508	0.0
22	0.0	0.0	0.0	1.4696823882548324	0.0
23	0.0	0.0	0.0	1.536448039422463	0.0
24	0.0	0.0	0.0	1.5856143766839819	0.0
25	0.0	0.0	0.0	1.6291936301657828	0.0
26	0.0	0.0	0.0	1.6724935294585976	0.0
27	0.0	0.0	0.0	1.7160727829403983	0.0
28	0.0	0.0	0.0	1.7628646095955371	0.0
29	0.0	0.0	0.0	1.8167799680698162	0.0
30	0.0	0.0	0.0	1.8848027130878835	0.0
31	0.0	0.0	0.0	1.9505906245940634	0.0
32	0.0	0.0	0.0	2.0105120981315396	0.0
33	0.0	0.0	0.0	2.064706810794805	0.0
34	0.0	0.0	0.0	2.123231513387351	0.0
35	0.0	0.0	0.0	2.2098313119729807	0.0
36	0.0	0.0	0.0	2.2827427552983015	0.0
37	0.0	0.0	0.0	2.346854541670566	0.0
38	0.0	0.0	0.0	2.4164137347280557	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	30	2.5263726E-6	44.000004	1
CCATGCG	30	2.5263726E-6	44.000004	1
ACATAGC	30	2.5263726E-6	44.000004	32
ACAACGT	25	4.4411536E-5	44.0	10
ACAACGC	20	7.8544905E-4	44.0	10
CGGAATA	25	4.4411536E-5	44.0	31
AACGTCG	20	7.8544905E-4	44.0	14
AACGTAA	20	7.8544905E-4	44.0	16
CGGAACA	25	4.4411536E-5	44.0	14
CTCACCG	20	7.8544905E-4	44.0	19
AAATCGT	20	7.8544905E-4	44.0	12
AGTTTCG	20	7.8544905E-4	44.0	10
CTCGTAG	20	7.8544905E-4	44.0	1
GATCGAC	20	7.8544905E-4	44.0	9
CGAAATC	25	4.4411536E-5	44.0	16
GGTCGGA	20	7.8544905E-4	44.0	9
CCGATCA	20	7.8544905E-4	44.0	38
TAGACCC	20	7.8544905E-4	44.0	30
GCGCGAC	50	2.7284841E-11	44.0	9
CGTGATG	25	4.4411536E-5	44.0	2
>>END_MODULE