##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549117_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3216406
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71901712656922	31.0	31.0	33.0	30.0	34.0
2	32.135306301505466	33.0	31.0	34.0	30.0	34.0
3	32.17864722301849	33.0	31.0	34.0	30.0	34.0
4	35.877018324179225	37.0	35.0	37.0	35.0	37.0
5	35.849909495256504	37.0	35.0	37.0	35.0	37.0
6	35.90968522008726	37.0	35.0	37.0	35.0	37.0
7	36.07728439755429	37.0	35.0	37.0	35.0	37.0
8	36.021997844799444	37.0	35.0	37.0	35.0	37.0
9	37.79420508480584	39.0	38.0	39.0	35.0	39.0
10	37.5931897279137	39.0	37.0	39.0	35.0	39.0
11	37.31479234897584	39.0	37.0	39.0	35.0	39.0
12	36.368710604320476	38.0	35.0	39.0	33.0	39.0
13	36.06812759334487	38.0	35.0	39.0	33.0	39.0
14	37.027651670840065	39.0	35.0	41.0	33.0	41.0
15	37.19943346704365	39.0	35.0	41.0	33.0	41.0
16	37.307445950542316	39.0	35.0	41.0	33.0	41.0
17	37.20633993345368	38.0	35.0	41.0	33.0	41.0
18	37.131488064628655	38.0	35.0	41.0	33.0	41.0
19	37.062224109767236	38.0	35.0	41.0	33.0	41.0
20	36.898547011788935	38.0	35.0	41.0	33.0	41.0
21	36.77573975424744	38.0	35.0	41.0	32.0	41.0
22	36.774852117549834	38.0	35.0	41.0	32.0	41.0
23	36.80005447073535	38.0	35.0	41.0	33.0	41.0
24	36.747832207749894	38.0	35.0	41.0	33.0	41.0
25	36.65417270083441	38.0	35.0	41.0	32.0	41.0
26	36.54925932857979	37.0	35.0	41.0	32.0	41.0
27	36.44981696962386	37.0	35.0	41.0	32.0	41.0
28	36.43913828042853	37.0	35.0	41.0	32.0	41.0
29	36.408432890623885	37.0	35.0	41.0	32.0	41.0
30	36.327603542587596	37.0	35.0	40.0	32.0	41.0
31	36.14076270222105	37.0	35.0	40.0	31.0	41.0
32	35.94118777293662	37.0	35.0	40.0	31.0	41.0
33	35.762104037860894	37.0	35.0	40.0	30.0	41.0
34	35.55027101678084	37.0	35.0	40.0	30.0	41.0
35	35.34565909900678	37.0	35.0	40.0	29.0	41.0
36	35.20830081774503	36.0	35.0	40.0	28.0	41.0
37	35.13180176880655	36.0	34.0	40.0	27.0	41.0
38	35.05753036152774	36.0	34.0	40.0	27.0	41.0
39	35.033724287294575	36.0	34.0	40.0	27.0	41.0
40	34.869003477794784	36.0	34.0	40.0	26.0	41.0
41	34.81192672815559	36.0	34.0	40.0	26.0	41.0
42	34.79604813571421	36.0	34.0	40.0	26.0	41.0
43	34.709197470717314	36.0	34.0	40.0	26.0	41.0
44	34.575606126838466	35.0	34.0	40.0	25.0	41.0
45	34.464707502721986	35.0	34.0	40.0	24.0	41.0
46	34.414998914937975	35.0	34.0	40.0	24.0	41.0
47	34.36209918772692	35.0	34.0	40.0	24.0	41.0
48	34.314520306205125	35.0	34.0	40.0	24.0	41.0
49	34.28282561343313	36.0	34.0	40.0	24.0	41.0
50	34.125721379701446	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	5.0
12	12.0
13	23.0
14	35.0
15	85.0
16	215.0
17	513.0
18	1227.0
19	2388.0
20	4299.0
21	7128.0
22	10687.0
23	15240.0
24	22244.0
25	32294.0
26	45945.0
27	57416.0
28	61107.0
29	63781.0
30	70655.0
31	82782.0
32	102333.0
33	137032.0
34	284506.0
35	606477.0
36	171637.0
37	221877.0
38	376927.0
39	837534.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.354773619996976	22.575663644452845	27.840297524628422	15.229265210921755
2	32.50441642006637	25.69656940075351	28.79770153394814	13.001312645231977
3	28.123999271236283	24.692374034869978	33.348961542790306	13.834665151103437
4	26.514718602067028	26.175209224208633	31.98585626317076	15.324215910553582
5	22.798147994998143	32.27148562712543	30.781872686470553	14.148493691405871
6	20.659052370876065	39.87475461742081	28.441154505992095	11.025038505711032
7	83.99489989758756	4.947820642045811	9.070403425438206	1.9868760349284265
8	85.88716722951021	3.9236651094420294	8.337877743046121	1.8512899180016453
9	81.32664222116239	5.1073465227959405	10.018231529228586	3.5477797268130953
10	52.43022802469589	22.226827085884057	14.920690982419508	10.422253907000547
11	46.581153001206935	18.82281030442052	21.775920079741176	12.82011661463136
12	42.918244773825194	19.56323299981408	24.912526590237675	12.605995636123051
13	22.45773698967108	39.16430326270999	24.030392929250848	14.34756681836808
14	14.965026181396254	39.706616639814754	29.843433944595304	15.484923234193692
15	15.147683470308163	21.747658722188678	49.50584596596325	13.598811841539904
16	17.689278032686172	17.892268575546744	46.03336767808542	18.38508571368167
17	17.347499040854917	18.13987413280537	30.613330531033707	33.899296295306
18	22.017183154116736	22.151867643574846	35.43498550867024	20.395963693638176
19	29.19292527124996	23.10476351555121	27.417527513628563	20.284783699570266
20	29.97214903839876	22.821870124604914	27.542947003581013	19.66303383341531
21	22.991997900762527	27.0535187411042	29.56868629146942	20.385797066663848
22	22.15118365032275	24.270163654712746	27.968204262770307	25.610448432194193
23	19.70447760637183	28.74755239232858	28.24870367733427	23.29926632396532
24	21.081604747659345	22.134114909622728	39.21454567613666	17.569734666581272
25	18.39926302836147	24.211868775272773	35.95873779616131	21.430130400204455
26	16.73513853661509	31.510854040192687	29.661056471104708	22.092950952087516
27	17.50882817654239	31.775372885139504	31.52916640498743	19.18663253333068
28	14.748884313734026	27.22075509124159	39.292054547840046	18.73830604718434
29	16.450814977959872	24.800600421712932	38.10669424195826	20.641890358368936
30	19.994024386224872	28.597322601686482	33.04128894175673	18.367364070331917
31	29.566323405689452	25.121735253571842	26.738446576707048	18.573494764031658
32	30.68449691985402	26.149683839664522	27.40443837003164	15.761380870449813
33	28.565050556428513	26.468704510562414	25.957046467392487	19.009198465616592
34	19.19188684513087	27.102704074050354	30.787686629113363	22.917722451705412
35	19.937377308710406	25.883517192792205	33.11913981008616	21.05996568841123
36	28.98328133948264	26.928037069947013	26.860321737989544	17.228359852580798
37	20.622614184900787	32.90256267399078	28.430708063596448	18.04411507751198
38	20.958361599872653	33.223915140066275	24.314219038268178	21.503504221792895
39	21.749617430137864	31.52863786474717	27.235958395799535	19.48578630931543
40	24.389707020817646	26.786388285558477	25.74255240165576	23.081352291968116
41	17.802292372293795	25.591545345954458	27.375461928624684	29.23070035312706
42	22.02330800278323	26.218362980295396	24.701297037749587	27.057031979171786
43	22.053901155513326	26.392128356930066	25.71043580940963	25.843534678146973
44	19.56152301668384	29.57030300279256	28.878754734321475	21.98941924620213
45	18.369105144064523	35.61935278071239	23.860420606105077	22.151121469118014
46	21.51174323142041	33.766073064159194	26.007071246602575	18.715112457817824
47	21.704878053330333	27.633047569243434	27.720256708885632	22.9418176685406
48	22.696916993687985	23.681805095501005	32.41416040139211	21.2071175094189
49	21.782262562624247	23.60208879102949	32.99788646085102	21.61776218549524
50	19.265571572742992	31.498448889847865	27.916314047418144	21.319665489991003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2591.0
1	5620.0
2	8649.0
3	27049.5
4	45450.0
5	31834.0
6	18218.0
7	17925.0
8	17632.0
9	17635.0
10	17638.0
11	17090.0
12	16542.0
13	15360.5
14	14179.0
15	13651.0
16	13123.0
17	12191.5
18	11260.0
19	10168.5
20	9077.0
21	10690.0
22	12303.0
23	13389.0
24	14475.0
25	18324.0
26	22173.0
27	26081.5
28	29990.0
29	36291.5
30	42593.0
31	45014.0
32	47435.0
33	58042.0
34	68649.0
35	73661.0
36	78673.0
37	88298.5
38	97924.0
39	123886.5
40	149849.0
41	192460.5
42	235072.0
43	281445.0
44	327818.0
45	340842.5
46	353867.0
47	307888.0
48	261909.0
49	259582.0
50	257255.0
51	239571.5
52	221888.0
53	206918.5
54	191949.0
55	176264.0
56	160579.0
57	149506.0
58	138433.0
59	126842.5
60	115252.0
61	100331.0
62	85410.0
63	70061.5
64	54713.0
65	42767.0
66	30821.0
67	24196.5
68	17572.0
69	13604.5
70	9637.0
71	8390.0
72	7143.0
73	5543.5
74	3944.0
75	3052.0
76	2160.0
77	1782.0
78	1404.0
79	964.0
80	524.0
81	431.0
82	338.0
83	248.0
84	158.0
85	113.0
86	68.0
87	54.5
88	41.0
89	28.0
90	15.0
91	11.5
92	8.0
93	5.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3216406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.360631815232058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.12548392067315	10.838877214966423
2	7.908342007016805	2.113208916493697
3	2.600614057440325	1.0423754074493081
4	1.1945399656072513	0.6383923467623379
5	0.7001384310094672	0.4677145898205871
6	0.47954730904932674	0.38442330205160125
7	0.36395326821724266	0.3403851930220685
8	0.2579227357134268	0.27568085669156
9	0.19930080673108788	0.2396506229291509
>10	2.418783817597321	7.760232333624486
>50	0.9686504867952497	9.354338992607058
>100	1.6284196693424735	52.65753717513344
>500	0.1481878363236585	11.674710801233955
>1k	0.005410032119752613	1.019356966671578
>5k	2.35218787815331E-4	0.17184128418301237
>10k+	4.70437575630662E-4	1.0212739963597939
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21047	0.6543639080389727	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	11450	0.3559873971134241	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC	5468	0.17000341374814	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT	3512	0.10919019551636204	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024064126232820112	0.0
2	0.0	0.0	0.0	0.10474423937774024	0.0
3	0.0	0.0	0.0	0.14528638486559223	0.0
4	0.0	0.0	0.0	0.22854701800705507	0.0
5	0.0	0.0	0.0	0.4778625583959239	0.0
6	0.0	0.0	0.0	0.6006393471470952	0.0
7	0.0	0.0	0.0	0.7215196091538195	0.0
8	0.0	0.0	0.0	0.9544193114923925	0.0
9	0.0	0.0	0.0	1.054779775936247	0.0
10	0.0	0.0	0.0	1.3932009827117597	0.0
11	0.0	0.0	0.0	1.536031209990281	0.0
12	0.0	0.0	0.0	1.7570853928266519	0.0
13	3.109060236798464E-5	0.0	0.0	1.8201060438265568	0.0
14	3.109060236798464E-5	0.0	0.0	1.8410300192202105	0.0
15	3.109060236798464E-5	0.0	0.0	1.8824427015743659	0.0
16	6.218120473596927E-5	0.0	0.0	1.951152932807612	0.0
17	6.218120473596927E-5	0.0	0.0	2.034133750527763	0.0
18	6.218120473596927E-5	0.0	0.0	2.1666108072177455	0.0
19	6.218120473596927E-5	0.0	0.0	2.2195270124480553	0.0
20	6.218120473596927E-5	0.0	0.0	2.2805889554987773	0.0
21	9.327180710395391E-5	0.0	0.0	2.3499209987793828	0.0
22	9.327180710395391E-5	0.0	0.0	2.425004803498066	0.0
23	9.327180710395391E-5	0.0	0.0	2.505902550859562	0.0
24	9.327180710395391E-5	0.0	0.0	2.566342681862924	0.0
25	1.2436240947193855E-4	0.0	0.0	2.6202848769713776	0.0
26	1.2436240947193855E-4	0.0	0.0	2.6748177935248223	0.0
27	1.2436240947193855E-4	0.0	0.0	2.732366498507962	0.0
28	1.2436240947193855E-4	0.0	0.0	2.7895421162626857	0.0
29	1.2436240947193855E-4	0.0	0.0	2.8500444284707838	0.0
30	1.2436240947193855E-4	0.0	0.0	2.9317816220962154	0.0
31	1.2436240947193855E-4	0.0	0.0	3.0082023227167216	0.0
32	1.2436240947193855E-4	0.0	0.0	3.078902352501519	0.0
33	1.2436240947193855E-4	0.0	0.0	3.1520274492710185	0.0
34	1.2436240947193855E-4	0.0	0.0	3.2318992067543713	0.0
35	1.2436240947193855E-4	0.0	0.0	3.3361459964942237	0.0
36	1.2436240947193855E-4	0.0	0.0	3.4214896999943414	0.0
37	1.2436240947193855E-4	0.0	0.0	3.502138722536894	0.0
38	1.2436240947193855E-4	0.0	0.0	3.5837204631504855	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAGT	65	0.0	44.000004	25
AACGCGA	65	0.0	44.000004	23
CGAACAC	25	4.4446315E-5	44.0	39
TCGATTG	75	0.0	44.0	1
CACGAAG	50	2.7284841E-11	44.0	1
TATGCGA	50	2.7284841E-11	44.0	12
TTATCCG	30	2.5291429E-6	44.0	23
CCCGTTA	25	4.4446315E-5	44.0	19
TAATTCG	20	7.858597E-4	44.0	20
CGCTAAT	25	4.4446315E-5	44.0	35
CTACCGT	20	7.858597E-4	44.0	40
TCACGAC	275	0.0	43.2	25
CGGTCTA	265	0.0	43.16981	31
CGTAATC	105	0.0	41.904762	33
CGTTTTT	13565	0.0	41.567272	1
TATCGTG	90	0.0	41.555557	1
CGATACA	85	0.0	41.411766	10
AACGAGC	380	0.0	41.105263	15
TCGTTGA	480	0.0	40.791664	24
AGTACGG	265	0.0	40.679245	2
>>END_MODULE