##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549116_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1390191
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67412103804441	31.0	31.0	33.0	30.0	34.0
2	32.1035936788542	33.0	31.0	34.0	30.0	34.0
3	32.184482563906684	33.0	31.0	34.0	30.0	34.0
4	35.890288456766015	37.0	35.0	37.0	35.0	37.0
5	35.87165576528693	37.0	35.0	37.0	35.0	37.0
6	35.92148201218394	37.0	35.0	37.0	35.0	37.0
7	36.065015526643464	37.0	35.0	37.0	35.0	37.0
8	35.99786144493814	37.0	35.0	37.0	35.0	37.0
9	37.74559682806176	39.0	38.0	39.0	35.0	39.0
10	37.544435980379674	39.0	37.0	39.0	35.0	39.0
11	37.28435229403729	39.0	37.0	39.0	34.0	39.0
12	36.64158018574426	39.0	35.0	39.0	33.0	39.0
13	36.44077972019672	39.0	35.0	39.0	33.0	39.0
14	37.500680122371676	40.0	35.0	41.0	33.0	41.0
15	37.6588929147146	40.0	35.0	41.0	33.0	41.0
16	37.7193838832218	40.0	35.0	41.0	33.0	41.0
17	37.665698454385044	39.0	35.0	41.0	33.0	41.0
18	37.60539738784095	39.0	35.0	41.0	33.0	41.0
19	37.509065301098914	39.0	35.0	41.0	33.0	41.0
20	37.373548670650294	39.0	35.0	41.0	33.0	41.0
21	37.2656304061816	39.0	35.0	41.0	33.0	41.0
22	37.24555115088502	39.0	35.0	41.0	33.0	41.0
23	37.217357902619135	39.0	35.0	41.0	33.0	41.0
24	37.19509693272364	39.0	35.0	41.0	33.0	41.0
25	37.12261336751569	39.0	35.0	41.0	33.0	41.0
26	37.018486668378664	39.0	35.0	41.0	33.0	41.0
27	36.93065125583463	38.0	35.0	41.0	33.0	41.0
28	36.89671203453339	38.0	35.0	41.0	32.0	41.0
29	36.849619224984195	38.0	35.0	41.0	32.0	41.0
30	36.75277569772786	38.0	35.0	41.0	32.0	41.0
31	36.59811133865778	38.0	35.0	41.0	31.0	41.0
32	36.453395972208135	38.0	35.0	41.0	31.0	41.0
33	36.30315402703657	38.0	35.0	41.0	31.0	41.0
34	36.13656468787382	38.0	35.0	41.0	30.0	41.0
35	35.93043761612613	38.0	35.0	41.0	30.0	41.0
36	35.761959327890914	38.0	35.0	41.0	29.0	41.0
37	35.68378733569704	38.0	35.0	41.0	29.0	41.0
38	35.61156920164208	38.0	35.0	41.0	29.0	41.0
39	35.58269834864418	38.0	35.0	41.0	29.0	41.0
40	35.408917911279815	38.0	35.0	40.0	27.0	41.0
41	35.318405888111776	38.0	35.0	40.0	27.0	41.0
42	35.28760148785311	38.0	35.0	40.0	27.0	41.0
43	35.22611784999327	38.0	35.0	40.0	27.0	41.0
44	35.10047468297522	37.0	34.0	40.0	27.0	41.0
45	35.009344759101445	37.0	34.0	40.0	26.0	41.0
46	34.95508171179356	37.0	34.0	40.0	26.0	41.0
47	34.90074097731895	37.0	34.0	40.0	26.0	41.0
48	34.843715719638524	37.0	34.0	40.0	26.0	41.0
49	34.76951224687831	36.0	34.0	40.0	26.0	41.0
50	34.625260845452175	36.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	3.0
13	6.0
14	22.0
15	28.0
16	72.0
17	149.0
18	434.0
19	818.0
20	1573.0
21	2477.0
22	3715.0
23	5479.0
24	8139.0
25	12437.0
26	17855.0
27	22265.0
28	23833.0
29	24898.0
30	28043.0
31	33177.0
32	41596.0
33	54883.0
34	108129.0
35	213022.0
36	80146.0
37	106673.0
38	182114.0
39	418201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.157248896015005	23.691420819153628	28.529245261981988	13.622085022849378
2	32.300309813543606	25.574039826182158	29.247779621649112	12.877870738625125
3	29.954445108621762	23.927215756683793	32.42561633617251	13.692722798521931
4	26.79790043238663	27.323655526470823	31.264408991282494	14.614035049860055
5	23.439656852907262	31.610188815781427	30.300153000558915	14.650001330752394
6	21.09350441773828	41.066659185680244	27.75870366014454	10.081132736436937
7	85.95588663715994	4.885731528976954	7.407543280024112	1.750838553839005
8	86.92776747943269	4.128353585946104	7.115497079178329	1.828381855442885
9	81.61979181277968	6.586361154690255	8.670679064963016	3.1231679675670465
10	47.01246087767796	27.074696930134063	14.853066952670533	11.059775239517448
11	39.95314312925346	21.53099825851268	24.46843635155169	14.047422260682167
12	38.37853935178691	21.417992203948955	26.698993159932698	13.504475284331432
13	21.30095792592529	37.88608903380902	25.40197713839321	15.410975901872476
14	14.566343761396816	39.65649324445346	28.86142983230362	16.915733161846106
15	15.043400511152786	24.549720146368376	44.59027572470258	15.816603617776263
16	16.204176260672096	19.903092452763683	43.73391857665601	20.158812709908208
17	16.41853529479043	21.359151368409087	30.12902543607317	32.093287900727304
18	20.434458286667084	23.58762213249834	34.511372897680964	21.46654668315361
19	26.125474844823483	25.76574010333832	26.469528287839584	21.639256763998617
20	27.63979913551447	22.69450744537981	28.99867716018878	20.66701625891694
21	21.52193475572781	26.96377691986209	30.208798647092376	21.30548967731772
22	22.649549594264386	24.584895169081083	26.78042081987295	25.98513441678158
23	18.76087530418482	29.637366376274915	27.9645027194105	23.637255600129766
24	18.12671783949112	24.385066512443252	39.26769774800729	18.220517900058336
25	16.557868666967344	27.287401515331343	34.66149615412558	21.493233663575726
26	17.866321965830593	32.13709483085418	28.90451743681264	21.09206576650259
27	18.0310475323175	32.18924593814807	29.659665470428166	20.120041059106267
28	15.086991643594297	27.824521954177516	37.15230497104355	19.936181431184636
29	16.44946629635784	24.13402187181474	36.732722338153536	22.683789493673892
30	17.859200642213914	30.645645094810714	32.044157961028375	19.450996301947
31	26.068576188451804	28.64318643984891	26.315808403305734	18.97242896839355
32	25.91802133663648	26.58145535397654	28.63218075789586	18.86834255149113
33	24.616617428828125	28.80827166914474	26.88947058353852	19.68564031848861
34	18.737713019290155	27.443423241842307	29.508175495309636	24.310688243557898
35	20.758442544945265	25.871265171476438	31.295987385905967	22.074304897672334
36	26.352925605186627	27.37609436401185	26.86163268212785	19.409347348673673
37	19.77828945806727	31.9088528123114	29.091110502082092	19.221747227539236
38	19.555154651411208	32.05674615934069	27.15936155535462	21.22873763389347
39	20.64076087386553	31.549981261567655	27.38120157589856	20.428056288668248
40	23.599706802878163	27.86645863769798	26.4638456154586	22.069988943965253
41	18.334674875610617	26.432339153396907	28.48328035500158	26.749705615990898
42	21.134218247708407	27.04132022146597	25.228403866806794	26.59605766401883
43	21.744637967013166	27.599229170667915	26.164318428187205	24.49181443413171
44	19.471209351808493	30.07040039821866	27.757480806594202	22.700909443378645
45	17.70397017388258	35.73221233629048	23.98620045734723	22.57761703247971
46	19.968623016549525	33.79945633369803	27.108792964419997	19.12312768533245
47	21.075809007539252	28.294817043125732	28.729361648866952	21.900012300468067
48	22.00855853620114	25.063822165443455	31.50603046631722	21.42158883203819
49	20.352023570861846	26.453559259123388	31.66924544900665	21.52517172100812
50	18.58500019062129	32.6063828639374	28.789641135642512	20.018975809798796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	745.0
1	1716.5
2	2688.0
3	9053.0
4	15418.0
5	11334.0
6	7250.0
7	6935.0
8	6620.0
9	6738.5
10	6857.0
11	6445.0
12	6033.0
13	5763.5
14	5494.0
15	5297.5
16	5101.0
17	4896.5
18	4692.0
19	4267.5
20	3843.0
21	4106.5
22	4370.0
23	4944.0
24	5518.0
25	6843.0
26	8168.0
27	12145.5
28	16123.0
29	18410.5
30	20698.0
31	26650.0
32	32602.0
33	35791.5
34	38981.0
35	44713.0
36	50445.0
37	51697.0
38	52949.0
39	60883.0
40	68817.0
41	90658.5
42	112500.0
43	128528.0
44	144556.0
45	142110.5
46	139665.0
47	132854.0
48	126043.0
49	116354.0
50	106665.0
51	103270.5
52	99876.0
53	90008.0
54	80140.0
55	70990.5
56	61841.0
57	54135.0
58	46429.0
59	41454.5
60	36480.0
61	31448.5
62	26417.0
63	21805.5
64	17194.0
65	14229.5
66	11265.0
67	8985.5
68	6706.0
69	5451.5
70	4197.0
71	3578.0
72	2959.0
73	2284.5
74	1610.0
75	1319.5
76	1029.0
77	795.0
78	561.0
79	402.0
80	243.0
81	229.0
82	215.0
83	147.5
84	80.0
85	74.0
86	68.0
87	43.0
88	18.0
89	14.5
90	11.0
91	9.0
92	7.0
93	5.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1390191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.375620426913322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.36941312126783	12.997233827807559
2	6.8294184845495165	2.2367192967905702
3	2.2815935033624615	1.1208752753872515
4	1.2086874347689258	0.7917202658622194
5	0.8383818689774176	0.6864511629590185
6	0.6226230024754534	0.6117502774561884
7	0.4758581898973924	0.5454731166358416
8	0.4113964767754267	0.538949803891508
9	0.38490143940327737	0.5672699886096585
>10	4.527728303826959	17.285917061223163
>50	1.39051866726753	16.534163568732975
>100	1.6483837631952551	43.58721325239271
>500	0.007988935847472964	0.8137239181507695
>1k	0.0026629786158243215	1.1178165292210465
>5k	4.438297693040536E-4	0.5647226548795533
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7770	0.5589160050669296	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4418	0.3177980579646969	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	3243	0.23327729786770307	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2490	0.17911207884384234	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2319	0.16681161077866277	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1661	0.11947998512434622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.031146799252764548	0.0
2	0.0	0.0	0.0	0.098907272453929	0.0
3	0.0	0.0	0.0	0.13811051862657722	0.0
4	0.0	0.0	0.0	0.1904054910440364	0.0
5	0.0	0.0	0.0	0.31851738358254367	0.0
6	0.0	0.0	0.0	0.42936546129272884	0.0
7	0.0	0.0	0.0	0.5094983351208575	0.0
8	0.0	0.0	0.0	0.7035723868159124	0.0
9	0.0	0.0	0.0	0.7924810331817714	0.0
10	0.0	0.0	0.0	0.9608751603196971	0.0
11	0.0	0.0	0.0	1.1073298561132967	0.0
12	0.0	7.193256178467562E-5	0.0	1.254719675210097	0.0
13	0.0	7.193256178467562E-5	0.0	1.3118341292671294	0.0
14	0.0	7.193256178467562E-5	0.0	1.3332700326789628	0.0
15	0.0	7.193256178467562E-5	0.0	1.3649203598642201	0.0
16	0.0	7.193256178467562E-5	0.0	1.4370687193342497	0.0
17	0.0	7.193256178467562E-5	0.0	1.5298617240364814	0.0
18	0.0	7.193256178467562E-5	0.0	1.667180984483427	0.0
19	0.0	7.193256178467562E-5	0.0	1.7304816388539417	0.0
20	7.193256178467562E-5	7.193256178467562E-5	0.0	1.795796404954427	0.0
21	7.193256178467562E-5	7.193256178467562E-5	0.0	1.8781591881978807	0.0
22	7.193256178467562E-5	7.193256178467562E-5	0.0	1.971239923147251	0.0
23	7.193256178467562E-5	7.193256178467562E-5	0.0	2.0773404517796474	0.0
24	7.193256178467562E-5	7.193256178467562E-5	0.0	2.153517034709619	0.0
25	7.193256178467562E-5	7.193256178467562E-5	0.0	2.2182563403158273	0.0
26	7.193256178467562E-5	7.193256178467562E-5	0.0	2.2804780062595715	0.0
27	7.193256178467562E-5	7.193256178467562E-5	0.0	2.347591086404674	0.0
28	7.193256178467562E-5	7.193256178467562E-5	0.0	2.4208903668632584	0.0
29	7.193256178467562E-5	7.193256178467562E-5	0.0	2.504979531589544	0.0
30	7.193256178467562E-5	7.193256178467562E-5	0.0	2.6092098136155393	0.0
31	7.193256178467562E-5	7.193256178467562E-5	0.0	2.7049520533509424	0.0
32	7.193256178467562E-5	7.193256178467562E-5	0.0	2.7851568597408556	0.0
33	7.193256178467562E-5	7.193256178467562E-5	0.0	2.8725549223092366	0.0
34	7.193256178467562E-5	7.193256178467562E-5	0.0	2.959161726697986	0.0
35	7.193256178467562E-5	7.193256178467562E-5	0.0	3.0792891048783946	0.0
36	7.193256178467562E-5	7.193256178467562E-5	0.0	3.1871160149936233	0.0
37	7.193256178467562E-5	7.193256178467562E-5	0.0	3.2919217575138955	0.0
38	7.193256178467562E-5	7.193256178467562E-5	0.0	3.3880236600582223	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.857051E-4	44.000004	1
AACCGTA	20	7.857051E-4	44.000004	21
TCGTTAG	40	8.314601E-9	44.000004	1
GATCGAC	20	7.857051E-4	44.000004	8
TAGCGTA	20	7.857051E-4	44.000004	42
CGCATCG	20	7.857051E-4	44.000004	21
TATATCG	40	8.314601E-9	44.000004	1
TAACGCG	40	8.314601E-9	44.000004	1
TCTAGCG	40	8.314601E-9	44.000004	1
GAGCGTA	20	7.857051E-4	44.000004	20
CGTAAAG	20	7.857051E-4	44.000004	12
CGGTAAT	20	7.857051E-4	44.000004	6
CGATACA	40	8.314601E-9	44.000004	10
CCCGAAC	20	7.857051E-4	44.000004	12
AGCGTTG	50	2.7284841E-11	44.0	1
CTATACG	25	4.4433225E-5	44.0	1
TATTGCG	30	2.5281006E-6	44.0	1
TAACCGG	75	0.0	44.0	2
TATTCGA	30	2.5281006E-6	44.0	26
CATCGTA	30	2.5281006E-6	44.0	33
>>END_MODULE