##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549114_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2423114
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.712851314465603	31.0	31.0	33.0	30.0	34.0
2	32.1524781747784	33.0	31.0	34.0	30.0	34.0
3	32.21071068055403	34.0	31.0	34.0	30.0	34.0
4	35.890366693436626	37.0	35.0	37.0	35.0	37.0
5	35.87067302652702	37.0	35.0	37.0	35.0	37.0
6	35.920094143321364	37.0	35.0	37.0	35.0	37.0
7	36.10515848614634	37.0	35.0	37.0	35.0	37.0
8	36.058197839639405	37.0	35.0	37.0	35.0	37.0
9	37.806490326084536	39.0	38.0	39.0	35.0	39.0
10	37.57558290695361	39.0	37.0	39.0	35.0	39.0
11	37.294037342031785	39.0	37.0	39.0	34.0	39.0
12	36.57043911264596	39.0	35.0	39.0	33.0	39.0
13	36.32848640220807	38.0	35.0	39.0	33.0	39.0
14	37.35207712059771	39.0	35.0	41.0	33.0	41.0
15	37.54083340693009	39.0	35.0	41.0	33.0	41.0
16	37.618083589959035	39.0	35.0	41.0	33.0	41.0
17	37.5567571315258	39.0	35.0	41.0	33.0	41.0
18	37.49194507563408	39.0	35.0	41.0	33.0	41.0
19	37.39981280286441	39.0	35.0	41.0	33.0	41.0
20	37.24704533092541	39.0	35.0	41.0	33.0	41.0
21	37.131235674425554	39.0	35.0	41.0	33.0	41.0
22	37.10381682413622	39.0	35.0	41.0	33.0	41.0
23	37.11174051241502	39.0	35.0	41.0	33.0	41.0
24	37.06925138478834	38.0	35.0	41.0	33.0	41.0
25	36.9636116996559	38.0	35.0	41.0	33.0	41.0
26	36.866756578518384	38.0	35.0	41.0	33.0	41.0
27	36.77507826705636	38.0	35.0	41.0	32.0	41.0
28	36.74345325890569	38.0	35.0	41.0	32.0	41.0
29	36.723576769396736	38.0	35.0	41.0	32.0	41.0
30	36.646631153136006	38.0	35.0	41.0	32.0	41.0
31	36.4859783732833	38.0	35.0	41.0	31.0	41.0
32	36.30725133031298	38.0	35.0	41.0	31.0	41.0
33	36.15152485603236	38.0	35.0	41.0	30.0	41.0
34	35.99125340367808	38.0	35.0	41.0	30.0	41.0
35	35.78365813577075	38.0	35.0	41.0	30.0	41.0
36	35.627345638711176	38.0	35.0	41.0	29.0	41.0
37	35.56642114238125	38.0	35.0	40.0	29.0	41.0
38	35.5181361669323	38.0	35.0	40.0	29.0	41.0
39	35.48579802683654	38.0	35.0	40.0	29.0	41.0
40	35.32958911549353	38.0	35.0	40.0	27.0	41.0
41	35.2526257534726	37.0	34.0	40.0	27.0	41.0
42	35.227105286833385	37.0	35.0	40.0	27.0	41.0
43	35.157734221336675	37.0	34.0	40.0	27.0	41.0
44	35.016431748568166	37.0	34.0	40.0	27.0	41.0
45	34.923309840147844	37.0	34.0	40.0	26.0	41.0
46	34.880262340112765	37.0	34.0	40.0	26.0	41.0
47	34.83272722620562	36.0	34.0	40.0	26.0	41.0
48	34.74895073034121	36.0	34.0	40.0	26.0	41.0
49	34.69379401877089	36.0	34.0	40.0	26.0	41.0
50	34.53956644218968	36.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	5.0
12	19.0
13	19.0
14	28.0
15	58.0
16	155.0
17	361.0
18	780.0
19	1510.0
20	2768.0
21	4562.0
22	6926.0
23	10206.0
24	14963.0
25	22442.0
26	31885.0
27	39409.0
28	42502.0
29	44714.0
30	49672.0
31	58261.0
32	72635.0
33	97104.0
34	191464.0
35	399019.0
36	136043.0
37	175880.0
38	303041.0
39	716674.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.91013712107643	23.859298406925962	29.23300348229592	14.99756098970168
2	32.104762714424496	25.78372292843011	29.141055682893995	12.970458674251399
3	28.78362305694243	24.812575883759493	32.33046402274098	14.07333703655709
4	25.930723853685794	27.157409845347765	31.538466617748895	15.373399683217546
5	23.228581073775313	32.06328715859014	30.662403832423895	14.045727935210643
6	21.010195970969587	40.62338792149276	27.548930838582088	10.817485268955567
7	86.13544389574737	4.310238808409345	7.720932651125782	1.8333846447175
8	87.5828376213418	3.6412649177876073	7.031613040079831	1.7442844207907675
9	82.33384809794339	5.455170495486387	9.107000330979062	3.103981075591161
10	49.525321549048044	25.656283608612718	14.369237270718587	10.44915757162065
11	42.11683808520771	20.03372519823665	24.007165985587143	13.842270730968497
12	38.66305918747529	21.439230675898862	26.808231061353283	13.089479075272562
13	21.232925896181527	37.847662140534865	26.244534924894165	14.674877038389445
14	15.262963277831748	39.728671453344745	29.78856958442731	15.219795684396194
15	14.512977928401222	23.57771858856001	46.71018367274507	15.199119810293697
16	16.70441423721707	19.537710565825627	44.48593834214981	19.271936854807493
17	16.450154635729067	19.546831061188207	31.680721583879258	32.322292719203475
18	21.08076631970266	22.92050642272712	35.076806126331654	20.921921131238562
19	27.331400833803116	23.771601336131937	28.892161078678097	20.00483675138685
20	29.015927438824583	22.84688215246992	28.768353449321822	19.36883695938367
21	22.190701716881666	26.719295914265693	30.139151521554496	20.950850847298145
22	21.89055075411227	24.122595965356975	28.0747005712484	25.912152709282353
23	19.489590667215822	27.988200307538154	28.449837688197917	24.07237133704811
24	19.090022178073337	24.12804350104865	38.99416205758375	17.787772263294258
25	17.565661376229098	25.561612041364956	36.1829447562104	20.68978182619555
26	17.216895284332477	32.84426568456952	29.422222809162097	20.516616221935905
27	18.227289347509032	32.38526953333603	30.67887024712828	18.708570872026655
28	15.174151938373514	27.855395990448656	38.38366663722796	18.586785433949867
29	16.34116265268576	24.97092584170617	37.33992705254479	21.34798445306329
30	18.140582737749856	30.063835213696095	32.23855749254884	19.557024556005207
31	27.525159773745685	26.35422848450382	27.13512447206363	18.985487269686857
32	28.077300531464882	26.228398663868063	28.44447269092581	17.249828113741245
33	25.209874566363776	27.73621051258835	27.631675604201867	19.42223931684601
34	19.294387304930762	26.96847940295009	30.622166352883106	23.11496693923604
35	21.158434972518833	24.630578668605768	32.35320335733275	21.857783001542643
36	27.68627476874798	26.447084206521033	27.872275097250892	17.994365927480093
37	19.732831389691118	32.04999847303924	30.239559508962433	17.97761062830721
38	19.685082913969378	32.22204980863467	27.019529415454656	21.073337861941287
39	21.23531951034908	30.894336791417988	28.528331725209792	19.342011973023144
40	22.78927033560947	27.612650498490787	27.4540529252854	22.14402624061435
41	18.10199602660048	26.265540952674947	28.80813696755497	26.8243260531696
42	20.987745520846314	26.618392696340333	26.364504517740393	26.02935726507296
43	21.826377132895935	26.597964437496547	27.63225337313886	23.94340505646866
44	19.662013425699325	29.94481481267493	29.4032389726608	20.989932788964943
45	17.885002521548717	35.73079929380128	25.25956269494543	21.12463548970457
46	20.969421991701587	33.32839478456234	26.754539819422448	18.94764340431362
47	20.952130192801494	28.340804435944822	29.261685583096792	21.44537978815689
48	21.849611698005127	25.1194949969337	31.783853339133035	21.247039965928142
49	20.778180473555928	25.666683449478644	32.60948515010024	20.945650926865184
50	18.98742692254677	31.54502842210478	29.584245726779674	19.883298928568777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1090.0
1	3440.5
2	5791.0
3	16873.0
4	27955.0
5	20502.5
6	13050.0
7	12685.0
8	12320.0
9	12237.0
10	12154.0
11	11876.5
12	11599.0
13	11034.0
14	10469.0
15	10019.0
16	9569.0
17	9005.0
18	8441.0
19	8457.5
20	8474.0
21	9183.5
22	9893.0
23	9465.0
24	9037.0
25	13869.5
26	18702.0
27	22573.5
28	26445.0
29	34279.0
30	42113.0
31	46522.0
32	50931.0
33	56911.5
34	62892.0
35	71428.5
36	79965.0
37	86591.5
38	93218.0
39	112949.0
40	132680.0
41	165277.5
42	197875.0
43	215362.0
44	232849.0
45	249094.5
46	265340.0
47	238815.0
48	212290.0
49	199588.5
50	186887.0
51	173005.5
52	159124.0
53	149404.5
54	139685.0
55	123852.5
56	108020.0
57	97455.5
58	86891.0
59	74854.0
60	62817.0
61	55613.5
62	48410.0
63	38983.0
64	29556.0
65	24232.5
66	18909.0
67	14378.0
68	9847.0
69	8262.5
70	6678.0
71	5733.0
72	4788.0
73	3730.0
74	2672.0
75	2126.0
76	1580.0
77	1410.0
78	1240.0
79	815.0
80	390.0
81	315.0
82	240.0
83	171.5
84	103.0
85	80.0
86	57.0
87	45.5
88	34.0
89	24.5
90	15.0
91	13.5
92	12.0
93	11.0
94	10.0
95	7.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2423114.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.785598191253712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.51147500327642	11.098988441803357
2	7.652896495757513	2.1099951217953326
3	2.4890005930238748	1.0293708621965805
4	1.1791320796718177	0.6502016425909215
5	0.700308583242663	0.48270863692347515
6	0.4845220440712782	0.40076557286229375
7	0.3468889461663714	0.3347450140185925
8	0.28759978026325045	0.3171788008481624
9	0.24789424102224844	0.3075633360592668
>10	2.9897506515612777	9.920245210321276
>50	1.0644799228218542	10.564507073693377
>100	2.0145832514686064	58.72309412748109
>500	0.02723227585342223	2.357469838323999
>1k	0.0036309701137896304	0.8179419654209408
>5k	3.0258084281580255E-4	0.29061153924665684
>10k+	3.0258084281580255E-4	0.5946128164146824
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14255	0.5882925854912315	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6967	0.2875225845750551	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3464	0.14295654269671176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01287599345305256	0.0
2	0.0	0.0	0.0	0.07634803810303602	0.0
3	0.0	0.0	0.0	0.12265209148228272	0.0
4	0.0	0.0	0.0	0.19470813176763455	0.0
5	0.0	0.0	0.0	0.3564008957069292	0.0
6	0.0	0.0	0.0	0.49324959535539803	0.0
7	4.126920978542487E-5	0.0	0.0	0.5924607756795595	0.0
8	4.126920978542487E-5	0.0	0.0	0.8145716627447162	0.0
9	4.126920978542487E-5	0.0	0.0	0.9265762981023592	0.0
10	4.126920978542487E-5	0.0	0.0	1.1225225061635564	0.0
11	4.126920978542487E-5	0.0	0.0	1.2677901246082521	0.0
12	4.126920978542487E-5	0.0	0.0	1.413965665668227	0.0
13	8.253841957084974E-5	0.0	0.0	1.471948905416749	0.0
14	8.253841957084974E-5	0.0	0.0	1.494688240008518	0.0
15	8.253841957084974E-5	0.0	0.0	1.526176647074797	0.0
16	8.253841957084974E-5	0.0	0.0	1.6011628012549142	0.0
17	8.253841957084974E-5	0.0	0.0	1.6904693712305736	0.0
18	8.253841957084974E-5	0.0	0.0	1.8286799548019614	0.0
19	8.253841957084974E-5	0.0	0.0	1.884310849592714	0.0
20	8.253841957084974E-5	0.0	0.0	1.945678164543641	0.0
21	8.253841957084974E-5	0.0	0.0	2.022191279485819	0.0
22	8.253841957084974E-5	0.0	0.0	2.104275737749029	0.0
23	8.253841957084974E-5	0.0	0.0	2.1939124614029715	0.0
24	8.253841957084974E-5	0.0	0.0	2.2667526166742467	0.0
25	8.253841957084974E-5	0.0	0.0	2.3245707795836266	0.0
26	8.253841957084974E-5	0.0	0.0	2.3832968651082864	0.0
27	8.253841957084974E-5	0.0	0.0	2.4414451816959497	0.0
28	1.2380762935627462E-4	0.0	0.0	2.5020283816609536	0.0
29	1.2380762935627462E-4	0.0	0.0	2.5678940404784916	0.0
30	1.2380762935627462E-4	0.0	0.0	2.6591815325238515	0.0
31	1.2380762935627462E-4	0.0	0.0	2.743329451276333	0.0
32	1.2380762935627462E-4	0.0	0.0	2.8207092196240047	0.0
33	1.2380762935627462E-4	0.0	0.0	2.8982540648108177	0.0
34	1.2380762935627462E-4	0.0	0.0	2.980173446234886	0.0
35	1.2380762935627462E-4	0.0	0.0	3.0833464706984484	0.0
36	1.2380762935627462E-4	0.0	0.0	3.1712911567511886	0.0
37	1.2380762935627462E-4	0.0	0.0	3.25300419212633	0.0
38	1.2380762935627462E-4	0.0	0.0	3.3365743419418155	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGCA	20	7.8582106E-4	44.000004	27
ACGTCCA	20	7.8582106E-4	44.000004	33
TCGCACA	25	4.4443048E-5	44.0	29
GTCGATA	45	4.802132E-10	44.0	1
GTCGACC	25	4.4443048E-5	44.0	38
CGCATCG	30	2.5288846E-6	44.0	21
CCAATCG	30	2.5288846E-6	44.0	28
ATTCGGT	25	4.4443048E-5	44.0	14
ATCGTCC	35	1.4469697E-7	44.0	10
ATCCGTT	30	2.5288846E-6	44.0	26
ATGTACG	55	1.8189894E-12	44.0	1
CGCTACT	45	4.802132E-10	44.0	17
CGGTATG	70	0.0	44.0	6
CAATCGT	25	4.4443048E-5	44.0	13
TGTCGAT	25	4.4443048E-5	44.0	15
TAATGCG	245	0.0	44.0	1
TCGCGAC	25	4.4443048E-5	44.0	38
CGCCCCA	25	4.4443048E-5	44.0	41
AATCGTC	55	1.8189894E-12	44.0	39
GTACGAT	25	4.4443048E-5	44.0	29
>>END_MODULE