##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549113_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	124926
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.664353297151912	31.0	31.0	33.0	30.0	34.0
2	32.101179898499915	33.0	31.0	34.0	30.0	34.0
3	32.17645646222564	33.0	31.0	34.0	30.0	34.0
4	35.87591053903911	37.0	35.0	37.0	35.0	37.0
5	35.845012247250374	37.0	35.0	37.0	35.0	37.0
6	35.89685093575397	37.0	35.0	37.0	35.0	37.0
7	36.05014968861566	37.0	35.0	37.0	35.0	37.0
8	35.983670332837036	37.0	35.0	37.0	35.0	37.0
9	37.67642444327041	39.0	38.0	39.0	35.0	39.0
10	37.52769639626659	39.0	37.0	39.0	35.0	39.0
11	37.280886284680534	39.0	37.0	39.0	34.0	39.0
12	36.42477946944591	38.0	35.0	39.0	33.0	39.0
13	36.15085730752605	38.0	35.0	39.0	33.0	39.0
14	37.15826969565983	39.0	35.0	41.0	33.0	41.0
15	37.34299505307142	39.0	35.0	41.0	33.0	41.0
16	37.41980052190897	39.0	35.0	41.0	33.0	41.0
17	37.33584682131822	39.0	35.0	41.0	33.0	41.0
18	37.288434753374	39.0	35.0	41.0	33.0	41.0
19	37.18405295935194	38.0	35.0	41.0	33.0	41.0
20	37.00708419384276	38.0	35.0	41.0	33.0	41.0
21	36.88505995549365	38.0	35.0	41.0	32.0	41.0
22	36.88523605974737	38.0	35.0	41.0	32.0	41.0
23	36.888550021612794	38.0	35.0	41.0	33.0	41.0
24	36.857443606615114	38.0	35.0	41.0	33.0	41.0
25	36.74291180378784	38.0	35.0	41.0	32.0	41.0
26	36.61096969405888	38.0	35.0	41.0	32.0	41.0
27	36.49407649328403	38.0	35.0	41.0	32.0	41.0
28	36.45348446280198	38.0	35.0	41.0	32.0	41.0
29	36.42945423690825	38.0	35.0	41.0	31.0	41.0
30	36.34213054128044	37.0	35.0	40.0	31.0	41.0
31	36.1635848422266	37.0	35.0	40.0	31.0	41.0
32	35.97972399660599	37.0	35.0	40.0	30.0	41.0
33	35.841842370683445	37.0	35.0	40.0	30.0	41.0
34	35.65599634983911	37.0	35.0	40.0	30.0	41.0
35	35.46642012071146	37.0	35.0	40.0	29.0	41.0
36	35.300473880537275	37.0	35.0	40.0	28.0	41.0
37	35.1920416886797	37.0	34.0	40.0	27.0	41.0
38	35.132334341930424	37.0	34.0	40.0	27.0	41.0
39	35.06960120391272	36.0	34.0	40.0	27.0	41.0
40	34.888878215903816	36.0	34.0	40.0	26.0	41.0
41	34.82899476490082	36.0	34.0	40.0	26.0	41.0
42	34.803139458559464	36.0	34.0	40.0	26.0	41.0
43	34.741198789683494	36.0	34.0	40.0	26.0	41.0
44	34.561556441413316	35.0	34.0	40.0	25.0	41.0
45	34.45290812160799	35.0	34.0	40.0	24.0	41.0
46	34.430742999855916	35.0	34.0	40.0	24.0	41.0
47	34.387501400829294	35.0	34.0	40.0	24.0	41.0
48	34.30566895602197	35.0	34.0	40.0	24.0	41.0
49	34.24203128251925	36.0	34.0	40.0	24.0	41.0
50	34.08396971006836	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	7.0
16	7.0
17	24.0
18	37.0
19	100.0
20	172.0
21	265.0
22	437.0
23	585.0
24	833.0
25	1263.0
26	1741.0
27	2116.0
28	2352.0
29	2584.0
30	2705.0
31	3220.0
32	3987.0
33	5442.0
34	10932.0
35	21897.0
36	7248.0
37	9145.0
38	15330.0
39	32494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.06917695275603	23.17932215871796	28.80505259113395	14.946448297392056
2	30.916702687991293	26.592542785328916	28.513680098618384	13.977074428061412
3	29.015577221715255	24.002209307910285	33.248483101996385	13.73373036837808
4	27.75563133374958	26.552519091302052	31.46742871780094	14.224420857147432
5	22.86553639754735	32.4071850535517	30.336359124601763	14.390919424299184
6	21.050061636488802	40.726510093975634	27.021596785296897	11.201831484238669
7	84.51963562428958	5.262315290652066	8.265693290427933	1.9523557946304213
8	85.50661991899204	4.232105406400589	8.10799993596209	2.153274738645278
9	80.17226197909162	6.623120887565438	9.571266189584234	3.633350943758705
10	51.73782879464643	21.90336679314154	15.077726013800168	11.28107839841186
11	45.88236235851625	19.678849879128443	21.493524166306454	12.94526359604886
12	40.27824472087475	21.53034596481117	24.687414949650194	13.503994364663882
13	21.408673934969503	38.462769959816214	25.29817652050014	14.830379584714152
14	14.823975793669852	40.04530682163841	30.546883755183067	14.58383362950867
15	15.716504170468918	20.954004770824326	48.18772713446361	15.14176392424315
16	17.223796487520612	18.559787394137327	44.343851560123596	19.872564558218464
17	16.85397755471239	18.878375998591164	30.020972415670077	34.24667403102637
18	22.093879576709412	22.551750636376735	34.69573987800778	20.65862990890607
19	29.577509885852425	22.89755535276884	26.98637593455325	20.538558826825483
20	29.335766773930168	22.233162031922898	27.318572594976224	21.11249859917071
21	21.966604229703986	27.219313833789606	29.023581960520627	21.79049997598578
22	22.386853016986056	23.828506475833695	26.666986856218884	27.117653650961373
23	20.11190624849911	28.279941725501494	27.34418775915342	24.26396426684597
24	20.475321390263034	22.592574804284133	39.07513247842723	17.8569713270256
25	18.09151017402302	25.330195475721627	35.16962041528585	21.408673934969503
26	17.058098394249395	32.25349406848855	28.512079150857307	22.17632838640475
27	18.519763700110463	31.335350527512283	30.26271552759233	19.882170244784913
28	15.640459151817875	27.91412516209596	38.04492259417575	18.400493091910413
29	16.773930166658662	24.05664153178682	36.41115540399917	22.758272897555354
30	19.080895890367096	29.14685493812337	31.91089124761859	19.861357923890942
31	28.419224180714984	26.307574083857645	25.376622960792787	19.896578774634584
32	29.01717816947633	25.353409218257205	27.68759105390391	17.94182155836255
33	26.66058306517458	26.79986552038807	25.95536557642124	20.584185838016104
34	19.245793509757778	26.869506747994816	30.280325952964155	23.604373789283255
35	21.90977058418584	24.047836319100906	31.337751949153898	22.704641147559354
36	29.313353505275124	26.60054752413429	25.43825944959416	18.647839520996428
37	20.91798344620015	32.95711060948081	28.329571106094807	17.79533483822423
38	21.708051166290442	33.13641675872117	23.492307446008038	21.663224628980355
39	22.51252741623041	30.82224677008789	26.695803915918226	19.969421897763475
40	23.719642028080624	25.942557994332642	25.888926244336645	24.448873733250085
41	18.422105886684918	25.258152826473275	27.207306725581542	29.112434561260265
42	22.18913596849335	26.285961289083136	24.791476554120038	26.733426188303476
43	22.78228711397147	26.12906840849783	26.182700158493827	24.90594431903687
44	20.546563565630855	29.984951091045897	27.313769751693002	22.154715591630243
45	18.060291692682068	35.80759809807406	23.996605990746524	22.135504218497353
46	22.145109905063798	32.13102156476634	26.000992587611865	19.722875942557994
47	23.1328946736468	26.17309447192738	28.25832893072699	22.43568192369883
48	23.469894177352995	24.19992635640299	30.82064582232682	21.5095336439172
49	21.411075356611114	24.91555000560332	31.75960168419704	21.913772953588527
50	19.806125226133872	32.574484094583994	27.28255127035205	20.336839408930086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	113.0
1	253.5
2	394.0
3	970.0
4	1546.0
5	1127.0
6	708.0
7	676.0
8	644.0
9	647.0
10	650.0
11	634.0
12	618.0
13	566.0
14	514.0
15	473.5
16	433.0
17	410.5
18	388.0
19	403.0
20	418.0
21	425.5
22	433.0
23	425.5
24	418.0
25	628.0
26	838.0
27	1007.0
28	1176.0
29	1630.5
30	2085.0
31	2006.5
32	1928.0
33	2318.5
34	2709.0
35	2753.5
36	2798.0
37	3276.5
38	3755.0
39	4494.0
40	5233.0
41	7328.0
42	9423.0
43	10703.5
44	11984.0
45	12177.5
46	12371.0
47	11505.5
48	10640.0
49	10656.0
50	10672.0
51	9719.0
52	8766.0
53	8141.5
54	7517.0
55	6984.0
56	6451.0
57	5791.5
58	5132.0
59	4730.0
60	4328.0
61	3789.0
62	3250.0
63	2752.5
64	2255.0
65	1895.0
66	1535.0
67	1267.5
68	1000.0
69	828.5
70	657.0
71	565.5
72	474.0
73	383.5
74	293.0
75	234.0
76	175.0
77	137.0
78	99.0
79	75.0
80	51.0
81	38.0
82	25.0
83	20.0
84	15.0
85	10.0
86	5.0
87	4.5
88	4.0
89	4.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	124926.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.42079310952084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.47819860747617	21.55275923346621
2	8.207002123351934	5.321550357811825
3	4.826922127302356	4.694779309351136
4	3.145523677843069	4.0792148952179685
5	2.53320823663029	4.106431007156237
6	1.943113920300232	3.779837663897027
7	1.6097970470594045	3.653362790772137
8	1.4295590341217719	3.7077950146486716
9	1.2246308824255592	3.5733154027184093
>10	8.557602093723766	43.045482925892124
>50	0.027159152634437803	0.5475241342874982
>100	0.014814083255147893	1.3015705297536142
>500	0.0024690138758579824	0.6363767350271361
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	795	0.6363767350271361	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	490	0.3922322014632663	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	409	0.32739381713974675	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	341	0.2729615932632118	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	154	0.12327297760274082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.031218481340953844	0.0
2	0.0	0.0	0.0	0.1056625522309207	0.0
3	0.0	0.0	0.0	0.1536909850631574	0.0
4	0.0	0.0	0.0	0.22813505595312425	0.0
5	0.0	0.0	0.0	0.39623457086595265	0.0
6	0.0	0.0	0.0	0.5859468805532876	0.0
7	0.0	0.0	0.0	0.7204264924835503	0.0
8	0.0	0.0	0.0	1.0630293133535054	0.0
9	0.0	0.0	0.0	1.2399340409522437	0.0
10	0.0	0.0	0.0	1.5593231192866177	0.0
11	0.0	0.0	0.0	1.7458335334518034	0.0
12	0.0	0.0	0.0	1.9475529513471976	0.0
13	0.0	0.0	0.0	2.012391335670717	0.0
14	0.0	0.0	0.0	2.0388069737284473	0.0
15	0.0	0.0	0.0	2.0724268767110128	0.0
16	0.0	0.0	0.0	2.162079951331188	0.0
17	0.0	0.0	0.0	2.248531130429214	0.0
18	0.0	0.0	0.0	2.4222339625058034	0.0
19	0.0	0.0	0.0	2.4694619214575027	0.0
20	0.0	0.0	0.0	2.5270960408561867	0.0
21	0.0	0.0	0.0	2.6015401117461536	0.0
22	0.0	0.0	0.0	2.6711813393528967	0.0
23	0.0	0.0	0.0	2.7536301490482367	0.0
24	0.0	0.0	0.0	2.8112642684469207	0.0
25	0.0	0.0	0.0	2.869698861726142	0.0
26	0.0	0.0	0.0	2.920929190080528	0.0
27	0.0	0.0	0.0	2.9721595184349137	0.0
28	0.0	0.0	0.0	3.0297936378335977	0.0
29	0.0	0.0	0.0	3.073819701263148	0.0
30	0.0	0.0	0.0	3.151465667675264	0.0
31	0.0	0.0	0.0	3.2315130557289917	0.0
32	0.0	0.0	0.0	3.3003538094551974	0.0
33	0.0	0.0	0.0	3.3763988281062387	0.0
34	0.0	0.0	0.0	3.446040055712982	0.0
35	0.0	0.0	0.0	3.53489265645262	0.0
36	0.0	0.0	0.0	3.6037334101788256	0.0
37	0.0	0.0	0.0	3.673374637785569	0.0
38	0.0	0.0	0.0	3.7510206041976852	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGACCG	75	0.0	44.000004	7
TAAGGGT	45	4.7293724E-10	44.000004	4
TAAGGGC	45	4.7293724E-10	44.000004	4
ATACATC	130	0.0	44.000004	23
GTTGAAG	20	7.829527E-4	44.0	1
CTATGCT	20	7.829527E-4	44.0	36
TAATAGG	25	4.4200176E-5	44.0	2
CGGCGCA	30	2.5095687E-6	44.0	22
GATACAT	125	0.0	44.0	22
ACACGGG	20	7.829527E-4	44.0	3
ATAGGGT	25	4.4200176E-5	44.0	4
TCACAGG	20	7.829527E-4	44.0	2
ACACCGG	30	2.5095687E-6	44.0	18
CATGAGG	20	7.829527E-4	44.0	2
TATGGCC	30	2.5095687E-6	44.0	10
ATAGCGG	30	2.5095687E-6	44.0	2
CACGCCA	30	2.5095687E-6	44.0	27
CAATTGG	25	4.4200176E-5	44.0	2
ACGGGTA	30	2.5095687E-6	44.0	5
ACGGGCG	20	7.829527E-4	44.0	5
>>END_MODULE