##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549112_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2717657
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68772512498818	31.0	31.0	33.0	30.0	34.0
2	32.11238025990771	33.0	31.0	34.0	30.0	34.0
3	32.15541181245462	33.0	31.0	34.0	30.0	34.0
4	35.867835418524116	37.0	35.0	37.0	35.0	37.0
5	35.863669329867605	37.0	35.0	37.0	35.0	37.0
6	35.92026403626359	37.0	35.0	37.0	35.0	37.0
7	36.08511964534156	37.0	35.0	37.0	35.0	37.0
8	36.024334196699584	37.0	35.0	37.0	35.0	37.0
9	37.75687881141734	39.0	38.0	39.0	35.0	39.0
10	37.563827223229424	39.0	37.0	39.0	35.0	39.0
11	37.33234216091287	39.0	37.0	39.0	35.0	39.0
12	36.63612185054994	39.0	35.0	39.0	33.0	39.0
13	36.41767448945912	39.0	35.0	39.0	33.0	39.0
14	37.466125416121315	40.0	35.0	41.0	33.0	41.0
15	37.61030659866201	40.0	35.0	41.0	33.0	41.0
16	37.66375153303011	40.0	35.0	41.0	33.0	41.0
17	37.57810606710118	39.0	35.0	41.0	33.0	41.0
18	37.498987914957624	39.0	35.0	41.0	33.0	41.0
19	37.390958461645454	39.0	35.0	41.0	33.0	41.0
20	37.220864884715034	39.0	35.0	41.0	33.0	41.0
21	37.1129149116316	38.0	35.0	41.0	33.0	41.0
22	37.09629544861622	39.0	35.0	41.0	33.0	41.0
23	37.08136494046158	38.0	35.0	41.0	33.0	41.0
24	37.04133671026182	38.0	35.0	41.0	33.0	41.0
25	36.9411117738552	38.0	35.0	41.0	33.0	41.0
26	36.84609500021526	38.0	35.0	41.0	33.0	41.0
27	36.73984906851748	38.0	35.0	41.0	32.0	41.0
28	36.67292009256503	38.0	35.0	41.0	32.0	41.0
29	36.61089534109713	38.0	35.0	41.0	32.0	41.0
30	36.49749066935232	38.0	35.0	41.0	31.0	41.0
31	36.31982034524592	38.0	35.0	41.0	31.0	41.0
32	36.11973806849061	38.0	35.0	41.0	30.0	41.0
33	35.933439356033524	38.0	35.0	41.0	30.0	41.0
34	35.70795063541867	38.0	35.0	41.0	29.0	41.0
35	35.47441306978769	38.0	35.0	41.0	28.0	41.0
36	35.33846876187834	38.0	35.0	40.0	27.0	41.0
37	35.242486818608825	38.0	35.0	40.0	27.0	41.0
38	35.16609417597585	38.0	34.0	40.0	27.0	41.0
39	35.10071837616005	37.0	34.0	40.0	26.0	41.0
40	34.95988787400323	37.0	34.0	40.0	25.0	41.0
41	34.85462109456786	37.0	34.0	40.0	24.0	41.0
42	34.838978943994775	37.0	34.0	40.0	24.0	41.0
43	34.76245604209803	37.0	34.0	40.0	24.0	41.0
44	34.627119242788915	37.0	34.0	40.0	24.0	41.0
45	34.545769756816256	36.0	34.0	40.0	23.0	41.0
46	34.50257813991979	36.0	34.0	40.0	23.0	41.0
47	34.43855681566879	36.0	34.0	40.0	23.0	41.0
48	34.34407579764481	36.0	34.0	40.0	23.0	41.0
49	34.266886144940294	36.0	34.0	40.0	24.0	41.0
50	34.11559920917173	36.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	8.0
12	12.0
13	22.0
14	30.0
15	69.0
16	212.0
17	499.0
18	922.0
19	1931.0
20	3569.0
21	5615.0
22	8738.0
23	12668.0
24	18770.0
25	27898.0
26	40401.0
27	50626.0
28	53383.0
29	55262.0
30	59645.0
31	68863.0
32	83464.0
33	110632.0
34	216426.0
35	411203.0
36	159456.0
37	208826.0
38	354471.0
39	764032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.193613837213455	23.47334487023197	27.779443837099382	16.553597455455197
2	32.04002565445161	25.940580433807504	29.19183693895146	12.827556972789427
3	29.82418311067217	24.239777131551186	33.366167989558654	12.569871768217991
4	27.096429019556183	26.47670401378835	32.4101974605331	14.01666950612237
5	23.180077544737987	31.9455692900171	31.29824698260303	13.576106182641887
6	20.428847349021602	41.06607272367337	28.08455960410015	10.420520323204878
7	83.8231609066192	4.766495551130992	9.314751640843564	2.095591901406248
8	85.30248666406393	3.6880665956005485	9.027114164885413	1.9823325754501027
9	80.258583036785	5.801983105299896	10.649283555651062	3.290150302264046
10	47.44844548079467	24.917677249189282	16.048603631731304	11.585273638284743
11	41.73668715367686	21.319025910922534	23.188761495656003	13.755525439744604
12	37.93120323867213	20.527903263730487	27.292443454048836	14.24845004354854
13	22.009142434089366	34.21127095877073	27.09720174400228	16.68238486313762
14	16.148432270886282	36.54335333708411	31.35314721467794	15.955067177351667
15	16.89017414633267	22.520833203012742	45.354067860660855	15.234924789993734
16	18.838764420969976	19.023592749195355	42.98548345137006	19.15215937846461
17	18.06294907709104	20.72586790753947	31.136269220140733	30.07491379522876
18	21.972713995916333	24.197056508602817	33.852542833771885	19.977686661708965
19	28.330175588751633	24.030111231844195	27.804171019374408	19.83554216002976
20	28.645079198736262	23.049413520543617	28.994865798001733	19.310641482718385
21	23.26544519783034	26.463751680215715	30.395815218771173	19.874987903182777
22	22.43870363331355	24.577273732483533	28.90784230681061	24.076180327392308
23	20.83272465951369	27.474512052109596	28.549371756627124	23.14339153174959
24	20.327215686159068	23.333113781466903	37.878032437500394	18.461638094873635
25	19.118196299238647	24.909582040706386	34.879162455011794	21.09305920504317
26	17.82160147509417	31.234883578023275	29.221568431925	21.721946514957555
27	19.34261755622582	29.774618356915532	30.863755065484717	20.019009021373925
28	16.627006277834177	27.16796122542322	37.2763008724059	18.9287316243367
29	18.44386543261346	25.10040818248955	35.974996108780466	20.48073027611652
30	19.891362302159543	28.68272191818173	31.973019406054554	19.452896373604176
31	28.00853823716532	25.786845065436882	27.148974281890613	19.055642415507183
32	28.36943734989368	25.614122753533653	28.37308019371098	17.643359702861694
33	25.98569282289855	27.59494667649376	27.046349116168816	19.373011384438875
34	19.54672719920137	27.403384606666698	30.2600364946717	22.789851699460232
35	21.772431178769065	25.523603604134003	31.41665780486647	21.28730741223046
36	26.593458997953018	27.830958800172358	26.923338743631003	18.65224345824363
37	21.204552303693955	32.52978576766678	28.034442904310588	18.231219024328677
38	21.34757991902584	32.23397949042134	25.482796394099772	20.935644196453048
39	22.481681831077285	30.52765672783578	27.364821977166358	19.625839463920574
40	23.912215559211482	26.4169466566237	26.792122773403708	22.87871501076111
41	18.025527136058745	26.916163445202983	27.944659682954843	27.113649735783437
42	21.681286490532102	26.864501296521233	25.5265841127118	25.927628100234873
43	22.645977766877863	26.34475211551715	26.697519223360416	24.311750894244565
44	20.32589101568005	29.448859808283384	27.90591307144353	22.319336104593038
45	18.596754483733598	34.34222935418266	25.107031534884644	21.95398462719909
46	20.770906703826128	32.602973811632594	26.93331056862584	19.69280891591544
47	22.40205441672735	27.12995054195581	28.402259740651598	22.065735300665242
48	22.31197682415404	25.022363013433996	31.529254795583107	21.136405366828853
49	21.10733620909482	26.27104892192061	31.856411607498664	20.765203261485905
50	19.921866519579183	30.816471688664173	28.64143635491896	20.62022543683769
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2281.0
1	5428.5
2	8576.0
3	27687.5
4	46799.0
5	33205.0
6	19611.0
7	18534.0
8	17457.0
9	17148.0
10	16839.0
11	15968.5
12	15098.0
13	14508.5
14	13919.0
15	12841.5
16	11764.0
17	11207.0
18	10650.0
19	10041.0
20	9432.0
21	9739.0
22	10046.0
23	11978.5
24	13911.0
25	15522.0
26	17133.0
27	25888.5
28	34644.0
29	42182.5
30	49721.0
31	54356.0
32	58991.0
33	60081.5
34	61172.0
35	68972.0
36	76772.0
37	79843.0
38	82914.0
39	99691.0
40	116468.0
41	148212.5
42	179957.0
43	214134.0
44	248311.0
45	244033.5
46	239756.0
47	227357.0
48	214958.0
49	210189.5
50	205421.0
51	194553.5
52	183686.0
53	177303.5
54	170921.0
55	155930.5
56	140940.0
57	131843.5
58	122747.0
59	114185.5
60	105624.0
61	92963.0
62	80302.0
63	64666.5
64	49031.0
65	39161.5
66	29292.0
67	23462.0
68	17632.0
69	15059.0
70	12486.0
71	10867.0
72	9248.0
73	7577.0
74	5906.0
75	4554.0
76	3202.0
77	2588.5
78	1975.0
79	1502.0
80	1029.0
81	798.0
82	567.0
83	409.5
84	252.0
85	176.0
86	100.0
87	82.0
88	64.0
89	45.0
90	26.0
91	16.5
92	7.0
93	11.0
94	15.0
95	8.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2717657.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.248064772027172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.15284383517002	12.221757544608513
2	6.840111520668842	2.085969270300956
3	2.293169502273094	1.0489919131169234
4	1.1985417630630835	0.7310176974066217
5	0.7474612641305141	0.5698668885021695
6	0.5458087351222477	0.49935161677693524
7	0.4296569934527021	0.4586006366144879
8	0.3734122130565892	0.4555050889082306
9	0.31141006604108046	0.4273560771890089
>10	4.147687491930124	15.208087419592406
>50	1.185008234208711	12.927133844390223
>100	1.754866253089447	49.34382822143763
>500	0.016115371150893253	1.577724118716622
>1k	0.0029300674819805915	0.8507036621803875
>5k	7.325168704951479E-4	0.7343178401323376
>10k+	2.441722901650493E-4	0.8597881601265565
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23093	0.8497393158886497	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	7105	0.2614384375953257	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	6352	0.23373074674250652	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6266	0.2305662561537383	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4914	0.18081752038612672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026934966406724617	0.0
2	0.0	0.0	0.0	0.09136546665013281	0.0
3	0.0	0.0	0.0	0.1327246227172892	0.0
4	0.0	0.0	0.0	0.1911573094029158	0.0
5	0.0	0.0	0.0	0.3289598356231121	0.0
6	0.0	0.0	0.0	0.469190924388177	0.0
7	0.0	0.0	0.0	0.5635368996161031	0.0
8	0.0	0.0	0.0	0.7584106456407118	0.0
9	0.0	0.0	0.0	0.8581656919912999	0.0
10	0.0	0.0	0.0	1.0254053399674794	0.0
11	0.0	0.0	0.0	1.169279272549847	0.0
12	0.0	0.0	0.0	1.3140731151870895	0.0
13	0.0	0.0	0.0	1.3705923889585772	0.0
14	0.0	0.0	0.0	1.390094482121916	0.0
15	0.0	0.0	0.0	1.4202307355196038	0.0
16	0.0	0.0	0.0	1.47932575744474	0.0
17	0.0	0.0	0.0	1.5506739813008044	0.0
18	0.0	0.0	0.0	1.661541541114276	0.0
19	0.0	0.0	0.0	1.70724267264044	0.0
20	0.0	0.0	0.0	1.7618485334977887	0.0
21	0.0	0.0	0.0	1.8325344221143434	0.0
22	0.0	0.0	0.0	1.9047289632208921	0.0
23	0.0	0.0	0.0	1.9879624249859347	0.0
24	0.0	0.0	0.0	2.0533496316864124	0.0
25	0.0	0.0	0.0	2.1085074385766855	0.0
26	0.0	0.0	0.0	2.16175183255282	0.0
27	0.0	0.0	0.0	2.2143706876916402	0.0
28	0.0	0.0	0.0	2.2753055297265257	0.0
29	0.0	0.0	0.0	2.3398464191765185	0.0
30	0.0	0.0	0.0	2.419621019135233	0.0
31	3.6796402194978986E-5	0.0	0.0	2.4918155602417817	0.0
32	3.6796402194978986E-5	0.0	0.0	2.5624646524561414	0.0
33	3.6796402194978986E-5	0.0	0.0	2.635137546791225	0.0
34	3.6796402194978986E-5	0.0	0.0	2.707957626735088	0.0
35	3.6796402194978986E-5	0.0	0.0	2.799286296983026	0.0
36	7.359280438995797E-5	0.0	0.0	2.8772578732341865	0.0
37	7.359280438995797E-5	0.0	0.0	2.956701305573146	0.0
38	7.359280438995797E-5	0.0	0.0	3.0400083601425787	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	25	4.444449E-5	44.0	21
TACGACA	30	2.5289974E-6	44.0	23
TACGAAC	25	4.444449E-5	44.0	12
CGTACGT	35	1.4470606E-7	44.0	27
ATTGCGA	20	7.85838E-4	44.0	10
ATTTCGA	45	4.802132E-10	44.0	13
TCGTAAG	180	0.0	42.77778	1
TATAGCG	240	0.0	41.25	1
CGTAAGG	365	0.0	40.9863	2
CGTTTTT	13285	0.0	40.803913	1
TAGTGCG	180	0.0	40.333332	1
ACAATCG	55	7.8216544E-11	40.0	37
CTATTCG	50	1.3496901E-9	39.600002	42
CAATACG	50	1.3496901E-9	39.600002	1
CGACGGT	380	0.0	39.36842	28
CGTATGT	45	2.3530447E-8	39.11111	23
ATCGCTA	45	2.3530447E-8	39.11111	40
CCGTTCG	45	2.3530447E-8	39.11111	42
CGTATGG	130	0.0	38.92308	2
CGGTCTA	380	0.0	38.789474	31
>>END_MODULE