##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549110_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	209704
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10153835882959	31.0	31.0	33.0	30.0	34.0
2	31.249346698203183	31.0	31.0	33.0	30.0	34.0
3	31.167388318773128	31.0	31.0	34.0	28.0	34.0
4	35.11988803265555	35.0	35.0	37.0	33.0	37.0
5	32.9231011330256	35.0	35.0	37.0	28.0	37.0
6	34.100441574791134	35.0	35.0	37.0	29.0	37.0
7	14.9624136878648	0.0	0.0	35.0	0.0	37.0
8	24.753566932438105	17.0	17.0	35.0	17.0	37.0
9	32.79050471140274	32.0	32.0	37.0	27.0	39.0
10	35.68087876244611	37.0	35.0	37.0	32.0	39.0
11	36.642405485827645	37.0	35.0	39.0	33.0	39.0
12	36.284329340403616	37.0	35.0	39.0	32.0	39.0
13	35.691603402891694	37.0	35.0	39.0	31.0	39.0
14	36.73863159501011	39.0	35.0	40.0	31.0	41.0
15	37.20517014458475	39.0	35.0	41.0	32.0	41.0
16	37.36739880975089	39.0	35.0	41.0	32.0	41.0
17	37.3525254644642	39.0	35.0	41.0	32.0	41.0
18	37.23773509327433	39.0	35.0	41.0	32.0	41.0
19	37.12276828291306	39.0	35.0	40.0	32.0	41.0
20	37.21295254263152	39.0	35.0	40.0	32.0	41.0
21	37.00895548010529	39.0	35.0	40.0	32.0	41.0
22	37.06231640788921	39.0	35.0	40.0	32.0	41.0
23	37.07228283676039	38.0	35.0	40.0	32.0	41.0
24	36.90375958493877	38.0	35.0	40.0	32.0	41.0
25	36.654756227825885	38.0	35.0	40.0	32.0	41.0
26	36.513786098500745	38.0	35.0	40.0	31.0	41.0
27	36.37094189905772	38.0	35.0	40.0	31.0	41.0
28	36.3382768092168	38.0	35.0	40.0	31.0	41.0
29	36.39138976843551	38.0	35.0	40.0	31.0	41.0
30	36.152224087284935	38.0	35.0	40.0	31.0	41.0
31	35.996089726471595	38.0	35.0	40.0	30.0	41.0
32	35.79101972303819	38.0	34.0	40.0	30.0	41.0
33	35.56245469805059	38.0	34.0	40.0	29.0	41.0
34	35.34492427421508	38.0	34.0	40.0	27.0	41.0
35	35.13180959829092	38.0	34.0	40.0	27.0	41.0
36	34.89634437111357	38.0	34.0	40.0	25.0	41.0
37	34.70524167397856	37.0	34.0	40.0	24.0	41.0
38	34.671041086483804	37.0	34.0	40.0	24.0	41.0
39	34.59077080074772	37.0	33.0	40.0	24.0	41.0
40	34.37505722351505	37.0	33.0	40.0	23.0	41.0
41	34.30040914813261	37.0	33.0	40.0	23.0	41.0
42	34.248822149315224	37.0	33.0	40.0	23.0	41.0
43	34.15647770190363	37.0	33.0	40.0	23.0	41.0
44	33.90033094266204	36.0	33.0	40.0	23.0	41.0
45	33.777243161789954	36.0	33.0	40.0	23.0	41.0
46	33.80616011139511	36.0	33.0	40.0	23.0	41.0
47	33.79041410750391	36.0	33.0	40.0	23.0	41.0
48	33.77867851829245	36.0	33.0	40.0	23.0	41.0
49	33.715498989051234	36.0	33.0	40.0	22.0	41.0
50	33.52640865219548	35.0	33.0	40.0	22.0	41.0
51	33.310280204478694	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	2.0
15	4.0
16	28.0
17	66.0
18	121.0
19	217.0
20	455.0
21	720.0
22	1131.0
23	1755.0
24	2592.0
25	3403.0
26	4084.0
27	4254.0
28	4426.0
29	5052.0
30	6016.0
31	7913.0
32	10781.0
33	17301.0
34	21893.0
35	21547.0
36	22686.0
37	35111.0
38	32750.0
39	5394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.525388166177088	23.227978483958342	28.749093961011713	16.497539388852857
2	32.368958150535995	24.049135924922748	31.785755159653604	11.796150764887651
3	28.529260290695458	22.541296303360927	36.33788578186396	12.591557624079655
4	25.6027543585244	26.77679014229581	32.1639072216076	15.456548277572196
5	20.99673825964216	36.23059169114561	30.118643421203217	12.654026628009001
6	20.020600465417925	40.08697974287567	29.23358638843322	10.658833403273185
7	36.66215236714607	57.7890741235265	4.926467783161026	0.622305726166406
8	87.81997482165337	3.0318925723877466	7.97266623431122	1.1754663716476557
9	84.62737954450083	4.0590546675313774	9.152901232213024	2.1606645557547783
10	58.792869950024794	17.833708465265328	13.662591080761455	9.710830503948422
11	54.846831724716736	16.282474344790753	17.728798687674054	11.14189524281845
12	47.87605386640217	19.868481287910576	21.873211765154693	10.38225308053256
13	20.212776103460115	46.26235074199824	21.86892000152596	11.655953153015679
14	13.95776904589326	47.54129630336093	26.51833059932095	11.982604051424865
15	11.39653988478999	20.914717888070804	54.91549994277648	12.773242284362722
16	13.16188532407584	14.087475680006104	53.78867355892114	18.961965436996913
17	13.457063289207646	14.676401022393467	31.003223591347805	40.86331209705108
18	20.88467554266967	21.053961774691945	37.26156868729256	20.79979399534582
19	30.164899095868464	22.71010567275779	25.74629000877427	21.37870522259947
20	33.40231945981002	20.26904589325907	25.536947316217145	20.791687330713767
21	20.647197955213063	28.349006218288636	29.00230801510701	22.001487811391293
22	21.695818868500364	22.762083698928013	26.559817647732043	28.982279784839584
23	18.699214130393315	30.041391675886008	25.048639987792313	26.210754205928353
24	19.11456147712967	21.494582840575287	42.76885514820891	16.62200053408614
25	16.10698889863808	22.51220768321062	40.00066760767558	21.380135810475718
26	15.18664403158738	35.32884446648609	28.35854347079693	21.12596803112959
27	15.67924312364094	35.02794414984931	31.01848319536108	18.274329531148666
28	13.533361309274024	27.981821996719187	43.10122839812307	15.38358829588372
29	13.736027925075344	23.271372982871092	42.0406859192004	20.951913172853164
30	17.31631251669019	30.24262770381109	34.970243772174115	17.47081600732461
31	31.595486972113072	24.988078434364628	25.25035287834281	18.16608171517949
32	32.01274176935108	24.797810246824096	27.270342959600196	15.919105024224622
33	29.665623927059094	26.86405600274673	25.159748979513978	18.3105710906802
34	18.853240758402322	27.685213443711138	28.972742532331285	24.48880326555526
35	19.93905695647198	24.667626750085837	32.679395719681075	22.71392057376111
36	33.450005722351506	23.01052912676916	26.305649868385917	17.23381528249342
37	20.498416816083623	32.779536871018195	29.368061648800214	17.353984664097965
38	20.066856140083164	34.058005569755466	22.98668599549842	22.888452294662955
39	21.137889596764964	30.896883225880288	27.747205585015067	20.21802159233968
40	24.147846488383625	25.34810971655286	25.56842024949453	24.93562354556899
41	16.987754167779347	23.24609926372411	28.243142715446535	31.523003853050014
42	23.367222370579483	23.871266165643	23.78304657994125	28.978464883836264
43	22.889882882539197	25.628028077671384	25.241769351085342	26.240319688704076
44	19.46314805630794	31.373268988669743	28.44914736962576	20.714435585396558
45	17.06452905047114	39.79323236562011	21.936157631709456	21.20608095219929
46	23.15120360126655	33.3760920154122	25.269904245984815	18.202800137336435
47	22.1683897302865	27.05718536603975	27.264620608095218	23.50980429557853
48	23.79687559607828	23.42635333613093	31.35753252203105	21.419238545759736
49	21.70058749475451	22.20653874031969	32.98792583832449	23.104947926601305
50	20.04492045931408	33.68176095830313	26.357627894556135	19.91569068782665
51	18.888051730057605	35.225842139396484	23.024835005531607	22.86127112501431
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	204.0
1	245.5
2	287.0
3	1309.0
4	2331.0
5	1786.0
6	1241.0
7	1338.5
8	1436.0
9	1509.0
10	1582.0
11	1568.0
12	1554.0
13	1520.5
14	1487.0
15	1347.0
16	1207.0
17	1077.0
18	947.0
19	963.5
20	980.0
21	907.5
22	835.0
23	867.5
24	900.0
25	980.5
26	1177.0
27	1293.0
28	1545.0
29	1797.0
30	2495.0
31	3193.0
32	3440.5
33	3688.0
34	3997.0
35	4306.0
36	5189.0
37	6072.0
38	8590.5
39	11109.0
40	15202.5
41	19296.0
42	21359.5
43	23423.0
44	22492.0
45	21561.0
46	19910.0
47	18259.0
48	16837.0
49	15415.0
50	14441.0
51	13467.0
52	12252.0
53	11037.0
54	9953.0
55	8869.0
56	8229.0
57	7589.0
58	6933.0
59	6277.0
60	5619.5
61	4962.0
62	4398.5
63	3835.0
64	3224.5
65	2614.0
66	2239.0
67	1864.0
68	1670.5
69	1477.0
70	1246.5
71	1016.0
72	801.0
73	586.0
74	460.5
75	242.5
76	150.0
77	117.0
78	84.0
79	64.5
80	45.0
81	31.5
82	18.0
83	12.5
84	7.0
85	6.0
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	209704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.298878419105026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.98712577131104	20.96621905161561
2	7.946979507884514	4.974630908327929
3	4.330006856098119	4.065730744287186
4	3.1126685457454104	3.8969213748903213
5	2.332596937609507	3.6503833975508333
6	1.866382265559534	3.504940296799298
7	1.5677611030700083	3.4348414908633123
8	1.4976765445265483	3.7500476862625414
9	1.209720423554506	3.4076603212146646
>10	9.09118610497448	46.27188799450654
>50	0.05027805286813438	1.0023652386220578
>100	0.0030471547192808712	0.13209094723991913
>500	0.004570732078921307	0.942280547819784
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	759	0.3619387326898867	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	654	0.31186815702132525	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	563	0.2684736581085721	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010967840384542021	0.0
2	0.0	0.0	0.0	0.035764696906115286	0.0
3	0.0	0.0	0.0	0.044825086788997826	0.0
4	0.0	0.0	0.0	0.06866821805974135	0.0
5	0.0	0.0	0.0	0.10252546446419715	0.0
6	0.0	0.0	0.0	0.14162819974821653	0.0
7	0.0	0.0	0.0	0.16070270476481135	0.0
8	0.0	0.0	0.0	0.3247434479075268	0.0
9	0.0	0.0	0.0	0.3862587265860451	0.0
10	0.0	0.0	0.0	0.5188265364513791	0.0
11	0.0	0.0	0.0	0.6308892534238737	0.0
12	0.0	0.0	0.0	0.7415213825201236	0.0
13	0.0	0.0	0.0	0.7844390188074619	0.0
14	0.0	0.0	0.0	0.8006523480715675	0.0
15	0.0	0.0	0.0	0.8354633197268531	0.0
16	0.0	0.0	0.0	0.9012703620341053	0.0
17	0.0	0.0	0.0	0.9742303437225804	0.0
18	0.0	0.0	0.0	1.0986914889558617	0.0
19	0.0	0.0	0.0	1.1540075535039866	0.0
20	0.0	0.0	0.0	1.207893030175867	0.0
21	0.0	0.0	0.0	1.2713157593560447	0.0
22	0.0	0.0	0.0	1.332831038034563	0.0
23	0.0	0.0	0.0	1.4129439591042612	0.0
24	0.0	0.0	0.0	1.4663525731507268	0.0
25	0.0	0.0	0.0	1.5049784458093312	0.0
26	0.0	0.0	0.0	1.5521878457254035	0.0
27	0.0	0.0	0.0	1.5912905810094229	0.0
28	0.0	0.0	0.0	1.6370693930492504	0.0
29	0.0	0.0	0.0	1.6828482050890778	0.0
30	0.0	0.0	0.0	1.7467477968946705	0.0
31	0.0	0.0	0.0	1.811124251325678	0.0
32	0.0	0.0	0.0	1.8650097279975584	0.0
33	0.0	0.0	0.0	1.922233243047343	0.0
34	0.0	0.0	0.0	1.9751649944683936	0.0
35	0.0	0.0	0.0	2.073398695303857	0.0
36	0.0	0.0	0.0	2.133960248731545	0.0
37	0.0	0.0	0.0	2.190706901155915	0.0
38	0.0	0.0	0.0	2.2479304162056994	0.0
39	0.0	0.0	0.0	2.30181589287758	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGGC	75	0.0	45.000004	4
AGGTCAA	35	1.2055898E-7	45.0	7
GTTGATC	55	1.8189894E-12	45.0	16
TTTAGCG	20	7.020086E-4	45.0	1
GTGCAAA	25	3.8798673E-5	45.0	1
ATGGGCT	45	3.8198777E-10	45.0	5
TTAGGCG	35	1.2055898E-7	45.0	5
TTAGGCC	40	6.7684596E-9	45.0	5
AACCGGA	20	7.020086E-4	45.0	24
GGTACTA	35	1.2055898E-7	45.0	38
TCGTTCA	20	7.020086E-4	45.0	16
CTCACGT	40	6.7684596E-9	45.0	45
GAAGCGC	20	7.020086E-4	45.0	9
ACACGCG	20	7.020086E-4	45.0	36
ACCGGAA	20	7.020086E-4	45.0	25
GAGCTTC	20	7.020086E-4	45.0	10
CCGATGT	35	1.2055898E-7	45.0	11
ACACCCA	20	7.020086E-4	45.0	30
TATGGGC	50	2.1827873E-11	45.0	4
AGGACTA	85	0.0	45.0	7
>>END_MODULE