FastQCFastQC Report
Sat 18 Jun 2016
SRR3549104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370559
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9930.26797352108571104No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.2655447580547228No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8540.23046262538489148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG207.0270913E-445.0000042
TCGTTAA207.0270913E-445.0000041
ATCTCCG207.0270913E-445.00000420
TCCGCTT207.0270913E-445.00000423
GGCGTAA207.0270913E-445.0000041
TGTATAG900.045.0000042
CGATTCG207.0270913E-445.00000410
ACGGGTG207.0270913E-445.0000045
CGTTCAC453.8380676E-1045.00000421
TATGAAC207.0270913E-445.00000427
CGTTAAG453.8380676E-1045.0000042
ACTAGGT207.0270913E-445.0000044
ATATCAC453.8380676E-1045.00000430
TCGTGCG207.0270913E-445.00000445
TCGCTAT207.0270913E-445.00000423
GTCAATC207.0270913E-445.00000418
GCTAGGC406.7939254E-945.0000044
ACGCTAG207.0270913E-445.0000042
TTAACGA207.0270913E-445.00000412
GCCTAGT207.0270913E-445.00000425