##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549104_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	370559
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03535469385442	31.0	31.0	33.0	30.0	33.0
2	31.192865373665192	31.0	31.0	33.0	30.0	34.0
3	31.129550220072918	31.0	31.0	34.0	28.0	34.0
4	35.09765786285045	35.0	35.0	37.0	32.0	37.0
5	32.853626547999106	35.0	35.0	37.0	28.0	37.0
6	34.02308674192234	35.0	35.0	37.0	28.0	37.0
7	15.290569113150672	0.0	0.0	35.0	0.0	37.0
8	24.90390194274056	17.0	17.0	35.0	17.0	37.0
9	32.71531928788668	32.0	32.0	37.0	27.0	39.0
10	35.56971224555334	37.0	35.0	37.0	32.0	39.0
11	36.525214608200045	37.0	35.0	39.0	32.0	39.0
12	36.2390037753772	37.0	35.0	39.0	32.0	39.0
13	35.783475775787394	37.0	35.0	39.0	31.0	39.0
14	36.810532195952604	39.0	35.0	40.0	31.0	41.0
15	37.238585488410756	39.0	35.0	41.0	32.0	41.0
16	37.33997015320097	39.0	35.0	41.0	32.0	41.0
17	37.318443216869646	39.0	35.0	41.0	32.0	41.0
18	37.23238404680496	39.0	35.0	40.0	32.0	41.0
19	37.15023518522017	39.0	35.0	40.0	32.0	41.0
20	37.24116537447478	39.0	35.0	40.0	32.0	41.0
21	37.05766153298125	39.0	35.0	40.0	32.0	41.0
22	37.11458094392526	39.0	35.0	40.0	32.0	41.0
23	37.12950434343789	39.0	35.0	40.0	32.0	41.0
24	36.947536019905066	38.0	35.0	40.0	32.0	41.0
25	36.700252861217784	38.0	35.0	40.0	31.0	41.0
26	36.542550578990124	38.0	35.0	40.0	31.0	41.0
27	36.465010969913024	38.0	35.0	40.0	31.0	41.0
28	36.40600282276237	38.0	35.0	40.0	31.0	41.0
29	36.446077412773676	38.0	35.0	40.0	31.0	41.0
30	36.19974956754525	38.0	35.0	40.0	30.0	41.0
31	36.03372472399807	38.0	35.0	40.0	30.0	41.0
32	35.902657876343575	38.0	34.0	40.0	30.0	41.0
33	35.715705191346046	38.0	34.0	40.0	29.0	41.0
34	35.54594814860791	38.0	34.0	40.0	28.0	41.0
35	35.354934032097454	38.0	34.0	40.0	27.0	41.0
36	35.17009437093688	38.0	34.0	40.0	26.0	41.0
37	35.00491959445055	38.0	34.0	40.0	25.0	41.0
38	34.94198764569205	38.0	34.0	40.0	25.0	41.0
39	34.88124428228703	38.0	34.0	40.0	25.0	41.0
40	34.674232713279125	37.0	33.0	40.0	24.0	41.0
41	34.58297329170254	37.0	34.0	40.0	24.0	41.0
42	34.53216626771985	37.0	33.0	40.0	24.0	41.0
43	34.459845800533785	37.0	33.0	40.0	23.0	41.0
44	34.22598020827992	37.0	33.0	40.0	23.0	41.0
45	34.1373708370327	36.0	33.0	40.0	23.0	41.0
46	34.13581103144168	36.0	33.0	40.0	23.0	41.0
47	34.083738352057296	36.0	33.0	40.0	23.0	41.0
48	34.07022633372823	36.0	33.0	40.0	23.0	41.0
49	34.00822810942387	36.0	33.0	40.0	23.0	41.0
50	33.823712283334096	36.0	33.0	40.0	23.0	41.0
51	33.64063752330938	35.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	3.0
15	15.0
16	47.0
17	118.0
18	211.0
19	445.0
20	711.0
21	1262.0
22	1949.0
23	2821.0
24	4024.0
25	5315.0
26	6502.0
27	7045.0
28	7728.0
29	8922.0
30	10871.0
31	14103.0
32	18998.0
33	29297.0
34	37486.0
35	37465.0
36	41760.0
37	63845.0
38	58464.0
39	11147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.143059539776395	24.75017473600695	28.77166659020561	14.335099134011047
2	32.95534584236248	24.30948917716207	30.153362892278963	12.581802088196481
3	31.08816679665047	22.94371476606964	33.27945077571993	12.688667661559968
4	26.92040943547451	27.004066828764113	30.74085368321914	15.33467005254224
5	20.92271406172836	37.29014812755864	28.31236051478982	13.474777295923188
6	20.61615019470584	41.44117401007667	28.33880704557169	9.603868749645805
7	37.96615383785038	57.082407929641434	4.18907650333685	0.762361729171333
8	87.82434106309657	3.612380214756625	6.941404742564612	1.6218739795821988
9	83.52893871151423	5.198902199109994	8.509845935465068	2.7623131539107133
10	55.164764585396654	21.130508232157364	13.331210414535876	10.373516767910102
11	51.128430290453075	18.620786433469434	18.39086353320254	11.859919742874954
12	44.831727201336356	20.99098928915503	22.040754643659984	12.136528865848623
13	22.106061382937668	42.79480460601416	22.302521325888726	12.796612685159447
14	14.866458512679493	44.9267188221039	27.079628345283744	13.127194319932858
15	14.18910348959275	22.22615022169209	49.906492623306946	13.678253665408208
16	15.615866839018889	15.826629497596874	49.53192339141675	19.02558027196749
17	16.05628253530477	16.11673174852048	29.7094389827261	38.117546733448656
18	21.1437314975483	22.92536411205773	35.46533750360941	20.46556688678456
19	31.25602130834766	22.381321193116346	25.987764431575002	20.374893066960997
20	33.510453126222814	22.38887734476831	23.5760027418036	20.524666787205277
21	23.01198999349631	28.143696415415633	27.68816841582582	21.15614517526224
22	23.023594083533254	24.22853040946246	24.325411068142994	28.42246443886129
23	20.109618171465275	30.006827522742668	24.538332627192972	25.34522167859909
24	20.247248076554612	23.179574642634506	39.998488769669606	16.574688511141275
25	18.057583272839143	23.407878367547408	37.47473411791374	21.059804241699702
26	16.048726383652806	34.186188973955566	28.70474067557393	21.060343966817697
27	18.017913476666333	33.590332443686435	29.90400988776416	18.487744191883074
28	14.984658313520924	28.391430244576437	40.3741374517958	16.24977399010684
29	16.16206865843226	24.862167697991413	38.1901397618193	20.78562388175702
30	19.15511430028686	30.333361219131096	31.4546401517707	19.056884328811336
31	31.056592877247617	25.265072498576473	24.487058740983215	19.19127588319269
32	31.078451744526514	25.37787504823793	26.987605212665187	16.556067994570363
33	29.93747284508	26.004226047673917	24.70834603936215	19.349955067883926
34	19.994926583890823	26.867246511351766	27.97908025442642	25.158746650330986
35	20.432913517145717	24.307060414131083	32.14629789048438	23.113728178238823
36	32.165727994732286	23.984844518686632	25.418084569528737	18.431342917052344
37	20.868201824810622	33.12589897964966	28.013622661978253	17.99227653356146
38	20.853359384065694	33.81188960462436	23.39141675144849	21.94333425986145
39	22.26042276668493	30.917883521922285	25.912472777614358	20.90922093377843
40	24.501361456610148	25.369239446349972	25.078057745190375	25.051341351849505
41	17.966099865338585	23.46293032958314	27.2763581507938	31.29461165428447
42	21.980845155562275	26.332109056857345	22.934539439063684	28.7525063485167
43	22.766954789925492	25.56785828977302	24.818449963433622	26.84673695686787
44	19.654629896993463	30.1862861244768	28.68207222061804	21.477011757911697
45	17.686522254215927	37.896259435069716	22.72998361934267	21.687234691371685
46	22.67763028289692	32.252084013611864	25.800479815629902	19.269805887861313
47	22.469296387349925	26.76712750196325	26.348300810397262	24.41527530028956
48	23.092948761195924	23.880137845795137	30.918423247040284	22.10849014596866
49	21.219562876626934	23.478852220564068	32.65552853931493	22.646056363494072
50	19.706173645762213	33.104849700047765	26.822989051675982	20.36598760251404
51	19.555590337840936	33.87287854295808	24.03557867977839	22.5359524394226
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	301.0
1	435.0
2	569.0
3	2066.5
4	3564.0
5	2708.0
6	1852.0
7	1896.5
8	1941.0
9	2008.0
10	2075.0
11	2118.5
12	2162.0
13	2016.0
14	1870.0
15	1800.5
16	1731.0
17	1590.0
18	1449.0
19	1414.0
20	1379.0
21	1519.5
22	1660.0
23	1746.0
24	1832.0
25	2030.5
26	2546.0
27	2863.0
28	3482.0
29	4101.0
30	4867.0
31	5633.0
32	6238.5
33	6844.0
34	7196.0
35	7548.0
36	9165.0
37	10782.0
38	15188.0
39	19594.0
40	25687.0
41	31780.0
42	34756.0
43	37732.0
44	36522.5
45	35313.0
46	33324.0
47	31335.0
48	29366.5
49	27398.0
50	26668.0
51	25938.0
52	23659.5
53	21381.0
54	19086.0
55	16791.0
56	15583.0
57	14375.0
58	13654.5
59	12934.0
60	11701.0
61	10468.0
62	9061.0
63	7654.0
64	6407.5
65	5161.0
66	4293.5
67	3426.0
68	3064.5
69	2703.0
70	2257.5
71	1812.0
72	1482.0
73	1152.0
74	927.0
75	488.5
76	275.0
77	188.5
78	102.0
79	87.0
80	72.0
81	58.5
82	45.0
83	34.5
84	24.0
85	15.0
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	370559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.480415191348545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.05227663753448	18.15143986333259
2	7.7451896288375135	4.256820534723632
3	4.399894179212013	3.627327565282315
4	2.9696780092911403	3.264319387197518
5	2.2829097413267383	3.1367653768016437
6	1.8221252548566695	3.0043655120461836
7	1.5912357921621527	3.060947416516525
8	1.4223168962370591	3.126868707381167
9	1.2154162641312787	3.0060129211780753
>10	10.333037284053475	51.352446059717074
>50	0.1419318334624099	2.46326058795422
>100	0.020989919033173293	0.7718289754852093
>500	0.0029985598618818992	0.7775970923838532
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	993	0.26797352108571104	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	984	0.2655447580547228	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	854	0.23046262538489148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009175327005955866	0.0
2	0.0	0.0	0.0	0.030494469166853323	0.0
3	0.0	0.0	0.0	0.037780758259818276	0.0
4	0.0	0.0	0.0	0.054512236917737794	0.0
5	0.0	0.0	0.0	0.09310258285455218	0.0
6	0.0	0.0	0.0	0.1362805922943445	0.0
7	0.0	0.0	0.0	0.16137781028122378	0.0
8	0.0	0.0	0.0	0.3421857248103541	0.0
9	0.0	0.0	0.0	0.4250335304229556	0.0
10	0.0	0.0	0.0	0.555916871537326	0.0
11	0.0	0.0	0.0	0.6633221700188094	0.0
12	0.0	0.0	0.0	0.7677589803513071	0.0
13	0.0	0.0	0.0	0.8095876769961059	0.0
14	0.0	0.0	0.0	0.8236205300640383	0.0
15	0.0	0.0	0.0	0.8497971982869125	0.0
16	0.0	0.0	0.0	0.9088970987076282	0.0
17	0.0	0.0	0.0	0.9839188901092674	0.0
18	0.0	0.0	0.0	1.106436491894678	0.0
19	0.0	0.0	0.0	1.1531227146014535	0.0
20	0.0	0.0	0.0	1.2057459136062003	0.0
21	0.0	0.0	0.0	1.2678143021759019	0.0
22	0.0	0.0	0.0	1.3282635153916111	0.0
23	0.0	0.0	0.0	1.4084126954142255	0.0
24	0.0	0.0	0.0	1.457797543710988	0.0
25	0.0	0.0	0.0	1.5015152782687777	0.0
26	0.0	0.0	0.0	1.5471220507395584	0.0
27	0.0	0.0	0.0	1.5889507473843572	0.0
28	0.0	0.0	0.0	1.6356369700911326	0.0
29	0.0	0.0	0.0	1.6855615435058924	0.0
30	0.0	0.0	0.0	1.7568052590815497	0.0
31	0.0	0.0	0.0	1.8188736476512513	0.0
32	0.0	0.0	0.0	1.8758146475999773	0.0
33	0.0	0.0	0.0	1.93761317361068	0.0
34	0.0	0.0	0.0	2.002919912888366	0.0
35	0.0	0.0	0.0	2.0930540075939326	0.0
36	0.0	0.0	0.0	2.161599097579603	0.0
37	0.0	0.0	0.0	2.229334599888277	0.0
38	0.0	0.0	0.0	2.2941016140479653	0.0
39	0.0	0.0	0.0	2.3639960168286294	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	20	7.0270913E-4	45.000004	2
TCGTTAA	20	7.0270913E-4	45.000004	1
ATCTCCG	20	7.0270913E-4	45.000004	20
TCCGCTT	20	7.0270913E-4	45.000004	23
GGCGTAA	20	7.0270913E-4	45.000004	1
TGTATAG	90	0.0	45.000004	2
CGATTCG	20	7.0270913E-4	45.000004	10
ACGGGTG	20	7.0270913E-4	45.000004	5
CGTTCAC	45	3.8380676E-10	45.000004	21
TATGAAC	20	7.0270913E-4	45.000004	27
CGTTAAG	45	3.8380676E-10	45.000004	2
ACTAGGT	20	7.0270913E-4	45.000004	4
ATATCAC	45	3.8380676E-10	45.000004	30
TCGTGCG	20	7.0270913E-4	45.000004	45
TCGCTAT	20	7.0270913E-4	45.000004	23
GTCAATC	20	7.0270913E-4	45.000004	18
GCTAGGC	40	6.7939254E-9	45.000004	4
ACGCTAG	20	7.0270913E-4	45.000004	2
TTAACGA	20	7.0270913E-4	45.000004	12
GCCTAGT	20	7.0270913E-4	45.000004	25
>>END_MODULE