FastQCFastQC Report
Sat 18 Jun 2016
SRR3549103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114366
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42030.37716513246096883No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29220.26221187652889627No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17810.15982181796644906No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17630.15820654973321152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT254.4427517E-544.013
CGAGTTA207.8563776E-444.035
ACGTTAC207.8563776E-444.038
ATTACGC302.5276477E-644.032
ACTTCGG502.7284841E-1144.012
CGTTATC207.8563776E-444.016
ATCGTAC254.4427517E-544.034
CCCGTAC351.4460238E-744.042
CGCTCGA254.4427517E-544.017
AACATCG207.8563776E-444.034
TGCGATA302.5276477E-644.01
GAAACGC207.8563776E-444.037
ACGCTCG207.8563776E-444.040
ACGCTAG302.5276477E-644.02
CGTAATT254.4427517E-544.020
CGTCGAA207.8563776E-444.017
ACGTATC302.5276477E-644.033
CCGAATA207.8563776E-444.028
GCTACGT207.8563776E-444.012
ATTGCGA207.8563776E-444.01
Produced by FastQC (version 0.11.5)