##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549103_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1114366 Sequences flagged as poor quality 0 Sequence length 50 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.537724589587263 31.0 31.0 33.0 30.0 34.0 2 31.720453603214743 31.0 31.0 34.0 30.0 34.0 3 31.773714381092027 31.0 31.0 34.0 30.0 34.0 4 35.67060104130959 37.0 35.0 37.0 35.0 37.0 5 27.024036088681815 35.0 25.0 37.0 0.0 37.0 6 31.296026619620484 35.0 26.0 37.0 17.0 37.0 7 15.05545933741697 0.0 0.0 32.0 0.0 37.0 8 25.145989737662493 17.0 17.0 35.0 17.0 37.0 9 33.265082567127855 32.0 32.0 37.0 28.0 39.0 10 36.10098208308581 37.0 35.0 39.0 32.0 39.0 11 37.05819362758734 39.0 37.0 39.0 34.0 39.0 12 36.90838288318201 39.0 35.0 39.0 33.0 39.0 13 36.315047300438096 39.0 35.0 39.0 32.0 39.0 14 37.61268829092058 40.0 36.0 41.0 33.0 41.0 15 37.934538562734325 40.0 36.0 41.0 33.0 41.0 16 38.03358860553893 40.0 36.0 41.0 33.0 41.0 17 38.06970869534785 40.0 36.0 41.0 34.0 41.0 18 38.05280760540074 40.0 36.0 41.0 34.0 41.0 19 37.97369535682173 40.0 36.0 41.0 34.0 41.0 20 37.986474820660355 40.0 36.0 41.0 34.0 41.0 21 37.825476548997365 40.0 35.0 41.0 34.0 41.0 22 37.75489740354605 39.0 35.0 41.0 33.0 41.0 23 37.66028306678416 39.0 35.0 41.0 33.0 41.0 24 37.522813869052 39.0 35.0 41.0 33.0 41.0 25 37.34618967197492 39.0 35.0 41.0 33.0 41.0 26 37.11035243358107 39.0 35.0 40.0 33.0 41.0 27 36.88751720709354 38.0 35.0 40.0 32.0 41.0 28 36.93694531240185 38.0 35.0 40.0 32.0 41.0 29 36.899145343630366 38.0 35.0 40.0 32.0 41.0 30 36.71894332741667 38.0 35.0 40.0 32.0 41.0 31 36.49290358822864 38.0 35.0 40.0 31.0 41.0 32 36.22419205180345 38.0 35.0 40.0 31.0 41.0 33 36.01885556450932 38.0 35.0 40.0 30.0 41.0 34 35.82063702589634 38.0 35.0 40.0 30.0 41.0 35 35.6599860369035 38.0 35.0 40.0 29.0 41.0 36 35.483139291758725 38.0 35.0 40.0 29.0 41.0 37 35.314165184508504 38.0 34.0 40.0 28.0 41.0 38 35.155197663963186 38.0 34.0 40.0 27.0 41.0 39 35.117200273518755 38.0 34.0 40.0 27.0 41.0 40 34.890517119151156 37.0 34.0 40.0 27.0 41.0 41 34.74784406559425 37.0 34.0 40.0 26.0 40.0 42 34.744776850693576 37.0 34.0 40.0 26.0 41.0 43 34.6300021716384 37.0 34.0 40.0 26.0 41.0 44 34.39546432680107 37.0 33.0 40.0 25.0 40.0 45 34.16244662884546 36.0 33.0 39.0 24.0 40.0 46 34.13184357742429 36.0 33.0 39.0 24.0 40.0 47 33.98552270977399 36.0 33.0 39.0 24.0 40.0 48 33.957277949973346 36.0 33.0 39.0 24.0 40.0 49 33.8406071254866 36.0 33.0 39.0 24.0 40.0 50 33.899349046902 36.0 33.0 39.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 2.0 13 1.0 14 9.0 15 18.0 16 67.0 17 146.0 18 362.0 19 779.0 20 1495.0 21 2356.0 22 3929.0 23 6127.0 24 9368.0 25 13693.0 26 17673.0 27 19294.0 28 20224.0 29 22599.0 30 28208.0 31 37762.0 32 53509.0 33 101410.0 34 98920.0 35 117639.0 36 143202.0 37 265857.0 38 122112.0 39 27602.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.48142890217397 23.71797057699176 28.957093091497764 14.843507429336503 2 32.925089243569886 24.19169285495071 30.31239287630814 12.570825025171265 3 28.934928021852784 23.227287982583817 34.70053824327016 13.137245752293234 4 26.724343707543124 26.394739250838594 32.042075942733355 14.838841098884927 5 17.20538853482608 48.47195625135727 23.686921532063973 10.635733681752674 6 20.27089843013875 40.171990171990174 28.587555614582644 10.969555783288435 7 39.13032163580009 55.73536881060621 4.1076271171231 1.026682436470603 8 87.80669905578597 2.619875337187244 7.83665330780013 1.7367722992266454 9 83.72985177221847 4.229579868732535 9.46304894442221 2.577519414626792 10 54.50399599413478 20.92579996159251 14.091959015260697 10.478245029012012 11 48.41407580633293 18.757661306967368 19.671992864103892 13.156270022595809 12 43.2672030553696 19.813687783008454 23.76256992765393 13.156539233968015 13 21.25737863502655 40.74855119413191 24.78018891459359 13.213881256247948 14 14.7300797045136 42.70293601922528 28.41068374304313 14.15630053321799 15 13.17547376714652 22.146404323175688 50.07116153938652 14.606960370291269 16 15.223633886891738 15.821821555934045 49.05435018656348 19.900194370610734 17 15.72732836428965 16.728704931772864 30.75434821234675 36.78961849159074 18 21.59595680413796 21.619108982147697 35.7386172944975 21.046316919216846 19 28.962387581817822 23.17021517167609 26.7462395658159 21.121157680690185 20 31.458066739293912 21.800557447014715 25.662394581313503 21.07898123237787 21 22.602986810437503 27.58967879493811 28.39130052424428 21.416033870380108 22 22.5783091013186 23.058402715086427 25.55156923308859 28.81171895050639 23 18.92753368282952 29.816415791580148 25.941297562919186 25.31475296267115 24 20.386390108815235 22.51962102217763 39.75946861264612 17.334520256361017 25 18.060583327201297 22.841149137715973 37.27240421908063 21.825863316002103 26 16.61886669191271 33.12143407103232 28.406466098211897 21.853233138843073 27 17.912337598239716 32.357860882331295 30.081678730327376 19.648122789101606 28 15.895764946166699 26.519563590418226 39.75282806546503 17.831843397950045 29 15.688472189567879 24.11380103125903 38.8985306443305 21.299196134842592 30 19.055139873255285 28.805168140449368 32.491658934317805 19.64803305197754 31 30.342365075747107 24.309338224604844 25.277153107686345 20.071143591961707 32 30.974204166315193 25.89983901159942 25.59168172754732 17.53427509453806 33 27.98676556894234 25.889878190827787 26.41214825290793 19.711207987321938 34 19.96731773941416 26.179908575818 29.008063777968818 24.84470990679902 35 20.266321836811247 24.509362274154093 32.238689981567994 22.985625907466666 36 30.103843799972363 23.47191138279524 26.507449078668948 19.916795738563454 37 19.979880936783786 31.638528095796175 28.10710305231854 20.274487915101503 38 21.55117797922765 32.648788638562195 23.641424810161112 22.15860857204904 39 20.588388375094 30.2947146628666 26.271709653740334 22.84518730829907 40 23.757095963085735 24.792303426342873 26.00519039525614 25.445410215315256 41 17.489765480999957 23.405954596604705 27.375566016910064 31.72871390548527 42 21.746445961201257 26.17263986876843 23.860742341385148 28.220171828645167 43 22.492251199336664 25.864213373344125 25.34140488851957 26.302130538799638 44 20.900583829729193 29.31020867470831 28.25804089500218 21.53116660056032 45 18.373227467456832 35.89126014253845 23.100758637646877 22.63475375235784 46 22.186427080510356 30.892453646288565 27.422049847177675 19.499069426023407 47 22.683750222099384 26.838309855110438 26.736727430664608 23.74121249212557 48 22.393360888612897 23.956402115642437 30.55270889456426 23.097528101180405 49 20.661075445589688 23.221993492263763 32.61163746919773 23.505293592948814 50 19.45276507000393 30.893351017529252 27.56006554399542 22.0938183684714 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 772.0 1 1185.0 2 1598.0 3 7203.0 4 12808.0 5 9390.0 6 5972.0 7 6170.0 8 6368.0 9 6770.5 10 7173.0 11 7108.5 12 7044.0 13 6725.0 14 6406.0 15 5931.0 16 5456.0 17 5123.0 18 4790.0 19 4525.5 20 4261.0 21 4266.5 22 4272.0 23 4640.0 24 5008.0 25 6164.0 26 7320.0 27 8381.5 28 9443.0 29 11076.5 30 12710.0 31 15210.5 32 17711.0 33 19650.5 34 21590.0 35 23146.5 36 24703.0 37 29975.0 38 35247.0 39 50763.0 40 66279.0 41 87155.0 42 108031.0 43 109624.0 44 111217.0 45 109578.5 46 107940.0 47 104403.5 48 100867.0 49 95104.5 50 89342.0 51 79967.5 52 70593.0 53 63173.0 54 55753.0 55 50752.5 56 45752.0 57 43375.5 58 40999.0 59 38289.0 60 35579.0 61 31658.5 62 27738.0 63 23111.0 64 18484.0 65 15593.0 66 12702.0 67 10664.5 68 8627.0 69 7151.0 70 5675.0 71 4647.0 72 3619.0 73 2903.0 74 2187.0 75 1670.0 76 1153.0 77 943.0 78 733.0 79 513.5 80 294.0 81 193.5 82 93.0 83 59.5 84 26.0 85 20.0 86 14.0 87 9.5 88 5.0 89 6.0 90 7.0 91 5.5 92 4.0 93 2.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1114366.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.947264971136693 #Duplication Level Percentage of deduplicated Percentage of total 1 74.05703449823481 15.512923246111358 2 6.478388470311305 2.7140903974713564 3 2.5852976149014757 1.6246474250576677 4 1.6279253516777072 1.3640233477929526 5 1.2381678258475395 1.2968114763382321 6 0.9969520846736752 1.253005168871195 7 0.8289040814055163 1.2154291400200607 8 0.7422905529000643 1.2439165517735364 9 0.6445105623410466 1.2150660173448795 >10 9.002645339624484 42.91428037342291 >50 1.6016357584135814 22.778972511955846 >100 0.19353475561808964 5.760881494045904 >500 9.043680169069609E-4 0.09538322368471269 >1k 0.0018087360338139217 1.0105696261094337 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4203 0.37716513246096883 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 2922 0.26221187652889627 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1781 0.15982181796644906 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1763 0.15820654973321152 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.011486351880800383 0.0 2 0.0 0.0 0.0 0.045048036282514005 0.0 3 0.0 0.0 0.0 0.06012387312606451 0.0 4 0.0 0.0 0.0 0.08758343309110292 0.0 5 0.0 0.0 0.0 0.1422333416489735 0.0 6 0.0 0.0 0.0 0.24480287445955817 0.0 7 0.0 0.0 0.0 0.3191949503125544 0.0 8 0.0 0.0 0.0 0.7231017457460116 0.0 9 0.0 0.0 0.0 0.8913588533749235 0.0 10 0.0 0.0 0.0 1.1645186590402077 0.0 11 0.0 0.0 0.0 1.3908356859416027 0.0 12 0.0 0.0 0.0 1.6165245529745165 0.0 13 0.0 0.0 0.0 1.7067103626636133 0.0 14 0.0 0.0 0.0 1.7328238657676203 0.0 15 0.0 0.0 0.0 1.783794552238672 0.0 16 0.0 0.0 0.0 1.9044909841111448 0.0 17 0.0 0.0 0.0 2.045468006023156 0.0 18 0.0 0.0 0.0 2.2761821520039196 0.0 19 0.0 0.0 0.0 2.3648424305838476 0.0 20 0.0 0.0 0.0 2.4567332456302506 0.0 21 0.0 0.0 0.0 2.569173862088398 0.0 22 0.0 0.0 0.0 2.6831400096557148 0.0 23 0.0 0.0 0.0 2.831834424237638 0.0 24 0.0 0.0 0.0 2.933596322931604 0.0 25 0.0 0.0 0.0 3.011757357995488 0.0 26 0.0 0.0 0.0 3.09799473422556 0.0 27 0.0 0.0 0.0 3.18360395058715 0.0 28 0.0 0.0 0.0 3.273341074655903 0.0 29 0.0 0.0 0.0 3.3665779465633374 0.0 30 0.0 0.0 0.0 3.4914022861429728 0.0 31 0.0 0.0 0.0 3.6070734390675954 0.0 32 0.0 0.0 0.0 3.7001308367268924 0.0 33 0.0 0.0 0.0 3.8049437976391958 0.0 34 0.0 0.0 0.0 3.9107438669162553 0.0 35 0.0 0.0 0.0 4.066886462795885 0.0 36 0.0 0.0 0.0 4.174929960174665 0.0 37 0.0 0.0 0.0 4.289165319114187 0.0 38 0.0 0.0 0.0 4.401336724200128 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACGT 25 4.4427517E-5 44.0 13 CGAGTTA 20 7.8563776E-4 44.0 35 ACGTTAC 20 7.8563776E-4 44.0 38 ATTACGC 30 2.5276477E-6 44.0 32 ACTTCGG 50 2.7284841E-11 44.0 12 CGTTATC 20 7.8563776E-4 44.0 16 ATCGTAC 25 4.4427517E-5 44.0 34 CCCGTAC 35 1.4460238E-7 44.0 42 CGCTCGA 25 4.4427517E-5 44.0 17 AACATCG 20 7.8563776E-4 44.0 34 TGCGATA 30 2.5276477E-6 44.0 1 GAAACGC 20 7.8563776E-4 44.0 37 ACGCTCG 20 7.8563776E-4 44.0 40 ACGCTAG 30 2.5276477E-6 44.0 2 CGTAATT 25 4.4427517E-5 44.0 20 CGTCGAA 20 7.8563776E-4 44.0 17 ACGTATC 30 2.5276477E-6 44.0 33 CCGAATA 20 7.8563776E-4 44.0 28 GCTACGT 20 7.8563776E-4 44.0 12 ATTGCGA 20 7.8563776E-4 44.0 1 >>END_MODULE