FastQCFastQC Report
Sat 18 Jun 2016
SRR3549099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874916
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36500.4171829067019005No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA24130.27579790517032493No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15840.18104595183994807No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.1687019096690425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG302.5270201E-644.0000043
TACCCGT302.5270201E-644.00000430
CTAACGG302.5270201E-644.0000042
ATTCACG302.5270201E-644.0000041
ATAACGG600.044.0000042
CCAACGG302.5270201E-644.0000042
GCTAATT302.5270201E-644.00000444
GACCTAG302.5270201E-644.0000042
CGGCGTA207.855449E-444.031
GGTACCC207.855449E-444.08
GCGCGTT207.855449E-444.017
GACGTGC254.441966E-544.034
TAGGTAT950.044.06
CGCATCG254.441966E-544.021
TGGAACG254.441966E-544.01
GGCAACG254.441966E-544.01
TCTTACG207.855449E-444.01
ATAGCGC207.855449E-444.037
ATAGCCG207.855449E-444.041
TGACGTT207.855449E-444.027
Produced by FastQC (version 0.11.5)