##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549099_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	874916
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58242848456309	31.0	31.0	33.0	30.0	34.0
2	31.777498639869428	31.0	31.0	34.0	30.0	34.0
3	31.79890526633414	31.0	31.0	34.0	30.0	34.0
4	35.69984204197889	37.0	35.0	37.0	35.0	37.0
5	27.210743659962784	35.0	26.0	37.0	0.0	37.0
6	31.403846769289853	35.0	28.0	37.0	17.0	37.0
7	14.879262694932999	0.0	0.0	32.0	0.0	37.0
8	25.04461914058035	17.0	17.0	35.0	17.0	37.0
9	33.23622039144329	32.0	32.0	37.0	28.0	39.0
10	36.16670628951808	37.0	35.0	39.0	32.0	39.0
11	37.1385024390913	39.0	37.0	39.0	34.0	39.0
12	36.92500422897741	39.0	35.0	39.0	33.0	39.0
13	36.30921254154685	39.0	35.0	39.0	32.0	39.0
14	37.62053957179889	40.0	35.0	41.0	33.0	41.0
15	37.95557287785342	40.0	36.0	41.0	33.0	41.0
16	38.044968888441865	40.0	35.0	41.0	33.0	41.0
17	38.09872376319555	40.0	35.0	41.0	34.0	41.0
18	38.062735165433025	40.0	36.0	41.0	34.0	41.0
19	37.98506828084067	40.0	36.0	41.0	34.0	41.0
20	37.98344641085544	40.0	36.0	41.0	34.0	41.0
21	37.81280488641195	40.0	35.0	41.0	34.0	41.0
22	37.72863566330939	39.0	35.0	41.0	33.0	41.0
23	37.633039057463805	39.0	35.0	41.0	33.0	41.0
24	37.47529705708891	39.0	35.0	41.0	33.0	41.0
25	37.29210575643833	39.0	35.0	41.0	33.0	41.0
26	37.05535960023591	39.0	35.0	40.0	33.0	41.0
27	36.81267458818904	38.0	35.0	40.0	32.0	41.0
28	36.86624087340956	38.0	35.0	40.0	32.0	41.0
29	36.86891770181366	38.0	35.0	40.0	33.0	41.0
30	36.68701109592235	38.0	35.0	40.0	32.0	41.0
31	36.4587171797064	38.0	35.0	40.0	31.0	41.0
32	36.16568219120464	38.0	35.0	40.0	31.0	41.0
33	35.933901083075405	38.0	35.0	40.0	30.0	41.0
34	35.70450420383214	38.0	35.0	40.0	30.0	41.0
35	35.550329403051265	38.0	35.0	40.0	29.0	41.0
36	35.35596560126915	38.0	35.0	40.0	28.0	41.0
37	35.14412355014653	38.0	34.0	40.0	27.0	41.0
38	35.00892199936908	38.0	34.0	40.0	27.0	41.0
39	34.98294007653306	37.0	34.0	40.0	27.0	41.0
40	34.7255839417727	37.0	34.0	40.0	26.0	41.0
41	34.587452966913396	37.0	34.0	40.0	25.0	40.0
42	34.578506965240095	37.0	34.0	40.0	25.0	41.0
43	34.44862592523168	37.0	33.0	40.0	24.0	41.0
44	34.23461566596107	36.0	33.0	40.0	24.0	40.0
45	33.986013514440245	36.0	33.0	39.0	23.0	40.0
46	33.990819690118826	36.0	33.0	39.0	23.0	40.0
47	33.87817116157437	36.0	33.0	39.0	23.0	40.0
48	33.840268094308485	36.0	33.0	39.0	23.0	40.0
49	33.7229105422692	36.0	33.0	39.0	24.0	40.0
50	33.746519665887924	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	7.0
14	7.0
15	17.0
16	49.0
17	141.0
18	319.0
19	605.0
20	1186.0
21	2002.0
22	3328.0
23	5136.0
24	7839.0
25	11672.0
26	14727.0
27	15811.0
28	16243.0
29	17755.0
30	21441.0
31	29035.0
32	41571.0
33	82153.0
34	78478.0
35	92424.0
36	108780.0
37	206414.0
38	96567.0
39	21208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.172935458946917	24.031907063078055	28.785963452491437	16.009194025483588
2	33.50721669280251	24.314791362827975	29.773029639416816	12.404962304952704
3	29.538492838169606	23.191712118649104	34.151164226051414	13.118630817129873
4	26.55134892949723	26.45328237225059	31.955753466618507	15.039615231633666
5	16.87121963708516	48.2814350177617	24.08254049531612	10.764804849837013
6	20.60414942691641	39.96109340782429	29.513118973707194	9.921638191552102
7	38.32550782017931	56.30151923156051	4.414709526400248	0.9582634218599272
8	86.89794220245145	3.198592779192517	8.351658902111746	1.5518061162443022
9	82.70622551193486	4.643988680056142	10.133315655445779	2.5164701525632176
10	56.99975769102406	18.921245010949622	14.435785835440202	9.643211462586123
11	52.06454105308396	17.41561475615945	18.967649465777285	11.552194724979312
12	45.977099515839235	19.683832505063343	22.86585226467455	11.47321571442287
13	20.85800236822735	43.43034074128259	23.61883883709979	12.092818053390268
14	13.843843294670574	45.35429686964234	27.891477581847855	12.910382253839225
15	12.849805009852375	21.06579374477093	53.24568301414079	12.838718231235912
16	13.857444600395924	14.929090335529354	52.375885227839014	18.837579836235708
17	14.439214736043231	16.008965432110053	30.05899994970946	39.49281988213726
18	20.954811661919546	21.73660099941023	36.041860018561785	21.266727320108444
19	29.45139876285266	22.893969249619392	26.755368515377477	20.89926347215047
20	31.85665823919096	20.833542877259074	25.65537720192567	21.654421681624292
21	21.445144448152735	26.549291589135414	30.322110922648577	21.683453040063274
22	23.019124121629964	23.017181077954913	25.97472214475447	27.98897265566066
23	19.77286962405534	29.04735997512904	25.19979060846984	25.97997979234578
24	19.7920714674323	21.790663332251327	41.547188530098886	16.870076670217486
25	17.227139519679604	22.619771498063816	38.681313406086986	21.4717755761696
26	16.089887486341546	33.39909202712032	28.853855684431416	21.657164802106717
27	17.5383693977479	32.85058222732239	30.30005166210242	19.31099671282729
28	14.74495837314668	27.58904854866067	41.32053820023865	16.345454877953998
29	14.264797992035808	23.932583242276973	40.30889822565823	21.493720540028985
30	18.24129402136891	28.442501908754668	33.67271829524206	19.643485774634364
31	29.92310118914273	25.221392682268927	25.427126718450687	19.42837941013766
32	30.54453227509841	25.036689236452414	27.71866099145518	16.700117496993997
33	28.23642498251261	25.378550626574437	26.239433271308332	20.145591119604624
34	19.36231592518596	27.666998889036204	29.31218539836967	23.658499787408164
35	20.19085260756461	24.866387173168626	31.98901380246789	22.95374641679887
36	33.02419889452244	22.450612401647703	25.260253555769925	19.264935148059926
37	20.888405286907545	31.941123490712254	28.512337184369702	18.658134038010505
38	21.779462256948097	32.33933314741072	23.60283730095232	22.278367294688863
39	21.720142276515688	29.988021707226753	26.60712571263984	21.68471030361772
40	23.930983088662224	24.88067425901458	26.073360185434947	25.114982466888247
41	17.526139652263762	23.603980267819995	27.831014634547774	31.03886544536847
42	23.51654330244275	25.2691686973378	24.17192050436842	27.042367495851032
43	22.76321383995721	26.180913367683296	25.296599902162036	25.759272890197458
44	20.452020536828677	30.118662820202168	27.83787243575383	21.59144420721532
45	16.957285042221194	37.28060750974951	22.741611766158123	23.02049568187117
46	22.04223033982691	31.731617663867162	27.147291854303727	19.078860142002206
47	23.912009838658797	26.561749927993088	26.791029081648983	22.735211151699133
48	24.339022260422716	22.51724737003324	30.099118086764904	23.04461228277915
49	21.54092507166402	23.164966693945477	32.677537043556185	22.61657119083432
50	19.905568077392573	32.90007269269278	26.211659176423797	20.98270005349085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	593.0
1	836.0
2	1079.0
3	6011.0
4	10943.0
5	8086.5
6	5230.0
7	5479.5
8	5729.0
9	6145.5
10	6562.0
11	6571.0
12	6580.0
13	6332.0
14	6084.0
15	5552.5
16	5021.0
17	4590.0
18	4159.0
19	3996.0
20	3833.0
21	3786.5
22	3740.0
23	3821.0
24	3902.0
25	4785.0
26	5668.0
27	6708.5
28	7749.0
29	8862.0
30	9975.0
31	11722.5
32	13470.0
33	14532.0
34	15594.0
35	16649.5
36	17705.0
37	22162.5
38	26620.0
39	40175.5
40	53731.0
41	69451.0
42	85171.0
43	89333.0
44	93495.0
45	90179.5
46	86864.0
47	80921.0
48	74978.0
49	71690.0
50	68402.0
51	61244.5
52	54087.0
53	47898.5
54	41710.0
55	37505.0
56	33300.0
57	31223.5
58	29147.0
59	28086.0
60	27025.0
61	24361.5
62	21698.0
63	18184.0
64	14670.0
65	12793.5
66	10917.0
67	9158.5
68	7400.0
69	6224.5
70	5049.0
71	4150.0
72	3251.0
73	2541.0
74	1831.0
75	1345.0
76	859.0
77	693.0
78	527.0
79	401.5
80	276.0
81	213.0
82	150.0
83	121.5
84	93.0
85	64.0
86	35.0
87	21.0
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	874916.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.11833216109938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51637162725116	15.525431553031124
2	6.216749399148608	2.625747575470706
3	2.578377856707885	1.6335312004434186
4	1.6559492321403517	1.3988354370502964
5	1.284719579192547	1.3565567403628014
6	1.0828642835692928	1.372097257548434
7	0.9046808949596972	1.3373746147691683
8	0.7731955447708706	1.3062880271962745
9	0.7123856861335492	1.3539957791922883
>10	9.67022939073417	46.514148990868044
>50	1.4700700965632363	20.414054627320873
>100	0.13216630201482582	4.082976501083315
>500	0.0	0.0
>1k	0.002240106813810607	1.0789616956632704
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3650	0.4171829067019005	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2413	0.27579790517032493	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1584	0.18104595183994807	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1476	0.1687019096690425	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0049147575310086915	0.0
2	0.0	0.0	0.0	0.028574171691910996	0.0
3	0.0	0.0	0.0	0.03920376356130188	0.0
4	0.0	0.0	0.0	0.05646256326321612	0.0
5	0.0	0.0	0.0	0.09692359037896209	0.0
6	0.0	0.0	0.0	0.1673303494278308	0.0
7	0.0	0.0	0.0	0.21739229823205886	0.0
8	0.0	0.0	0.0	0.47936030430349885	0.0
9	0.0	0.0	0.0	0.5985717486021516	0.0
10	0.0	0.0	0.0	0.8125351462311811	0.0
11	0.0	0.0	0.0	0.9941525815049673	0.0
12	0.0	0.0	0.0	1.1635402712946157	0.0
13	0.0	0.0	0.0	1.2357757773317668	0.0
14	0.0	0.0	0.0	1.257492147817619	0.0
15	0.0	0.0	0.0	1.2921240439082151	0.0
16	0.0	0.0	0.0	1.3809325695266745	0.0
17	0.0	0.0	0.0	1.4833424008704836	0.0
18	0.0	0.0	0.0	1.6576448481911406	0.0
19	0.0	0.0	0.0	1.7202794325398096	0.0
20	0.0	0.0	0.0	1.7877144777327194	0.0
21	0.0	0.0	0.0	1.8769801900982495	0.0
22	0.0	0.0	0.0	1.9605310681253971	0.0
23	0.0	0.0	0.0	2.0638552729633473	0.0
24	0.0	0.0	0.0	2.1389481961696895	0.0
25	0.0	0.0	0.0	2.1902674085283618	0.0
26	1.1429668676764398E-4	0.0	0.0	2.258159640468342	0.0
27	1.1429668676764398E-4	0.0	0.0	2.315307983852164	0.0
28	1.1429668676764398E-4	0.0	0.0	2.3737135907904303	0.0
29	1.1429668676764398E-4	0.0	0.0	2.433147867909605	0.0
30	1.1429668676764398E-4	0.0	0.0	2.522413580275135	0.0
31	1.1429668676764398E-4	0.0	0.0	2.604250008000768	0.0
32	1.1429668676764398E-4	0.0	0.0	2.673856690242263	0.0
33	1.1429668676764398E-4	0.0	0.0	2.7489496134486053	0.0
34	1.1429668676764398E-4	0.0	0.0	2.831471821294844	0.0
35	1.1429668676764398E-4	0.0	0.0	2.9443969478212764	0.0
36	1.1429668676764398E-4	0.0	0.0	3.0226901782571125	0.0
37	1.1429668676764398E-4	0.0	0.0	3.1090984734534515	0.0
38	1.1429668676764398E-4	0.0	0.0	3.184762880093632	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	30	2.5270201E-6	44.000004	3
TACCCGT	30	2.5270201E-6	44.000004	30
CTAACGG	30	2.5270201E-6	44.000004	2
ATTCACG	30	2.5270201E-6	44.000004	1
ATAACGG	60	0.0	44.000004	2
CCAACGG	30	2.5270201E-6	44.000004	2
GCTAATT	30	2.5270201E-6	44.000004	44
GACCTAG	30	2.5270201E-6	44.000004	2
CGGCGTA	20	7.855449E-4	44.0	31
GGTACCC	20	7.855449E-4	44.0	8
GCGCGTT	20	7.855449E-4	44.0	17
GACGTGC	25	4.441966E-5	44.0	34
TAGGTAT	95	0.0	44.0	6
CGCATCG	25	4.441966E-5	44.0	21
TGGAACG	25	4.441966E-5	44.0	1
GGCAACG	25	4.441966E-5	44.0	1
TCTTACG	20	7.855449E-4	44.0	1
ATAGCGC	20	7.855449E-4	44.0	37
ATAGCCG	20	7.855449E-4	44.0	41
TGACGTT	20	7.855449E-4	44.0	27
>>END_MODULE