##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549094_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452718
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03860239707721	31.0	31.0	33.0	30.0	33.0
2	31.182080235378315	31.0	31.0	33.0	30.0	34.0
3	31.1339752340309	31.0	31.0	34.0	28.0	34.0
4	35.097137732539906	35.0	35.0	37.0	32.0	37.0
5	32.85232749747083	35.0	35.0	37.0	28.0	37.0
6	34.035152125605784	35.0	35.0	37.0	28.0	37.0
7	15.240708343825515	0.0	0.0	35.0	0.0	37.0
8	24.875770347103494	17.0	17.0	35.0	17.0	37.0
9	32.71326521145614	32.0	32.0	37.0	27.0	39.0
10	35.56099161067154	37.0	35.0	37.0	32.0	39.0
11	36.52061106472462	37.0	35.0	39.0	32.0	39.0
12	36.27846253075866	37.0	35.0	39.0	32.0	39.0
13	35.85315803657023	37.0	35.0	39.0	31.0	39.0
14	36.90506673028243	39.0	35.0	40.0	31.0	41.0
15	37.30129131158911	39.0	35.0	41.0	32.0	41.0
16	37.36849208558087	39.0	35.0	41.0	32.0	41.0
17	37.33860372240556	39.0	35.0	41.0	32.0	41.0
18	37.25965612147076	39.0	35.0	40.0	32.0	41.0
19	37.167017878679445	39.0	35.0	40.0	32.0	41.0
20	37.27673518614237	39.0	35.0	40.0	32.0	41.0
21	37.12158783171864	39.0	35.0	40.0	32.0	41.0
22	37.16186014251698	39.0	35.0	41.0	32.0	41.0
23	37.16960006008155	39.0	35.0	41.0	32.0	41.0
24	37.002204462822334	39.0	35.0	40.0	32.0	41.0
25	36.74204471657853	38.0	35.0	40.0	31.0	41.0
26	36.61362481721513	38.0	35.0	40.0	31.0	41.0
27	36.51201189261306	38.0	35.0	40.0	31.0	41.0
28	36.43300244302193	38.0	35.0	40.0	31.0	41.0
29	36.452250628426526	38.0	35.0	40.0	31.0	41.0
30	36.226341784510446	38.0	35.0	40.0	30.0	41.0
31	36.07683149333581	38.0	35.0	40.0	30.0	41.0
32	35.94476914989022	38.0	34.0	40.0	30.0	41.0
33	35.78064048701399	38.0	34.0	40.0	29.0	41.0
34	35.623394696035945	38.0	34.0	40.0	29.0	41.0
35	35.439381690147066	38.0	34.0	40.0	27.0	41.0
36	35.239449281892924	38.0	34.0	40.0	26.0	41.0
37	35.092503501075726	38.0	34.0	40.0	26.0	41.0
38	35.00542501071307	38.0	34.0	40.0	26.0	41.0
39	34.93211889078852	38.0	34.0	40.0	25.0	41.0
40	34.765637770090876	37.0	34.0	40.0	24.0	41.0
41	34.649693628263066	37.0	34.0	40.0	24.0	41.0
42	34.60535476831051	37.0	33.0	40.0	24.0	41.0
43	34.50658246413882	37.0	33.0	40.0	23.0	41.0
44	34.278124572029384	37.0	33.0	40.0	23.0	41.0
45	34.15457083659143	37.0	33.0	40.0	23.0	41.0
46	34.14970467266598	36.0	33.0	40.0	23.0	41.0
47	34.11745943390808	36.0	33.0	40.0	23.0	41.0
48	34.08808132214756	36.0	33.0	40.0	23.0	41.0
49	33.99540773726691	36.0	33.0	40.0	23.0	41.0
50	33.86055778652494	36.0	33.0	40.0	23.0	41.0
51	33.65299369585482	36.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	2.0
14	6.0
15	19.0
16	43.0
17	125.0
18	282.0
19	535.0
20	847.0
21	1404.0
22	2251.0
23	3321.0
24	4946.0
25	6324.0
26	7650.0
27	8359.0
28	9232.0
29	11053.0
30	13207.0
31	17304.0
32	23109.0
33	35386.0
34	45624.0
35	45949.0
36	52091.0
37	79106.0
38	71094.0
39	13446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.026961596402174	24.136879911998196	28.358934259296078	14.477224232303554
2	32.435202488083085	24.579318692872825	30.522974566948964	12.462504252095123
3	31.60444250062953	23.28623999929316	32.318131817157706	12.79118568291961
4	28.011919119628555	26.970211036450948	30.24333028507813	14.77453955884237
5	20.582128388975036	37.196223697754455	28.741733264416258	13.479914648854255
6	20.716207440393358	40.9789316969946	28.335520125110996	9.96934073750105
7	38.052606699976586	56.99861724075473	4.148940399984096	0.7998356592845878
8	88.32474078786353	3.194262211796306	6.886626995171387	1.5943700051687806
9	84.1121846270747	4.96423822335317	8.318644277453071	2.604932872119068
10	53.91391550589992	21.592249479808622	13.190330404357681	11.303504609933777
11	48.933331566228865	19.319532247447636	18.453651058716463	13.293485127607033
12	42.62410595558383	20.531765911671283	23.10754156008818	13.736586572656709
13	22.276118908459573	39.618703033676596	23.701288660932413	14.403889396931424
14	16.0554694092128	42.74802415631806	26.72436262750763	14.472143806961506
15	14.462424732394116	22.043744671075594	48.02437720611948	15.46945339041081
16	15.790845515309751	16.576986114976652	48.08423786993228	19.547930499781323
17	16.398287675771673	16.939242530670306	29.468675864445416	37.19379392911261
18	21.33292689930597	22.958221232643723	34.600568124086074	21.108283743964236
19	30.09732327850892	23.172703537301366	25.644220022177162	21.085753162012555
20	31.712456761162578	22.377727415300473	24.247103053114742	21.662712770422203
21	23.231680648880758	27.56682968205373	27.908985284437556	21.292504384627957
22	22.57277157082334	23.648275526928465	25.04671782434098	28.73223507790722
23	19.87418216196396	30.51524348490672	24.800206751222614	24.810367601906705
24	20.612831829085657	23.93565089084154	38.107828714564036	17.34368856550877
25	18.77261341497356	23.186177708860704	36.79177766291598	21.24943121324975
26	16.949403381354397	32.93153795519507	27.654522241218594	22.46453642223194
27	18.545319602931627	32.10232418414996	29.14750462760482	20.204851585313595
28	15.515839882664265	28.171400297757103	39.10712629053848	17.205633529040153
29	16.435396869574436	25.227183368012756	37.028127885350266	21.30929187706254
30	19.209750882447793	28.94958892732341	32.04047552781202	19.800184662416783
31	31.19756669714922	24.905128578938765	24.295919314010046	19.60138540990197
32	30.371224470862657	25.299855539209837	27.526186279317365	16.802733710610138
33	28.947380046739912	25.037440525890286	25.106799376212123	20.908380051157675
34	19.934705489951803	26.670245053211932	28.30017803577503	25.09487142106123
35	20.844985178411285	23.361121051073734	32.13766627348592	23.656227497029057
36	31.54237295623324	23.947578845992428	25.77299776019509	18.737050437579246
37	20.862656223079266	32.03075645324462	27.338431429720046	19.76815589395606
38	21.621627591569144	33.512031772538315	22.48375368330837	22.38258695258417
39	21.747533784828523	30.094672621808723	25.262746345407074	22.89504724795568
40	24.528514439452373	24.503112312742147	25.512128963283985	25.45624428452149
41	18.638313475496886	23.31605988717038	27.067622670183205	30.978003967149526
42	22.06450814856047	26.87103229825189	22.41086062405294	28.653598929134695
43	22.457026228248047	25.561166112237636	24.467549335347833	27.51425832416648
44	20.45401331513216	29.696632340662397	27.999328500302617	21.850025843902827
45	19.073462950445975	36.025958764617265	23.243166827914948	21.657411457021812
46	22.712151935642055	31.46108615076052	26.348411152196288	19.478350761401135
47	22.567028481306245	27.484880212405955	26.040493198856684	23.907598107431117
48	23.308328805128138	24.550161469170654	29.888584063368366	22.252925662332842
49	21.47252815218304	23.951113054926022	32.06786564704739	22.50849314584355
50	20.508572665544555	31.30204674874867	26.53108557645157	21.65829500925521
51	20.179228570545018	32.946116567046154	24.226560463688212	22.648094398720616
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	233.0
1	437.5
2	642.0
3	2532.0
4	4422.0
5	3365.0
6	2308.0
7	2317.5
8	2327.0
9	2388.0
10	2449.0
11	2442.5
12	2436.0
13	2270.0
14	2104.0
15	2060.5
16	2017.0
17	1899.5
18	1782.0
19	1728.0
20	1674.0
21	1718.0
22	1762.0
23	1843.5
24	1925.0
25	2231.0
26	3090.5
27	3644.0
28	4313.0
29	4982.0
30	5636.5
31	6291.0
32	7222.0
33	8153.0
34	8563.5
35	8974.0
36	11094.5
37	13215.0
38	18061.5
39	22908.0
40	30212.0
41	37516.0
42	40312.5
43	43109.0
44	42144.0
45	41179.0
46	39674.5
47	38170.0
48	35919.5
49	33669.0
50	32040.5
51	30412.0
52	28165.5
53	25919.0
54	23998.0
55	22077.0
56	20872.0
57	19667.0
58	18257.5
59	16848.0
60	15415.5
61	13983.0
62	12073.5
63	10164.0
64	8461.0
65	6758.0
66	5906.5
67	5055.0
68	4470.0
69	3885.0
70	3518.5
71	3152.0
72	2670.0
73	2188.0
74	1697.5
75	835.5
76	464.0
77	363.0
78	262.0
79	201.5
80	141.0
81	93.5
82	46.0
83	36.0
84	26.0
85	18.5
86	11.0
87	10.5
88	10.0
89	11.5
90	13.0
91	7.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	452718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.02278124841017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.38867839563898	20.138524341929145
2	7.355292182470738	4.2694207205997925
3	3.907445575224938	3.4021481450946482
4	2.7715730909946523	3.2175503813567126
5	2.088282100323607	3.030387729133129
6	1.6760581522516953	2.91863214674493
7	1.3185213841810235	2.6787010393105777
8	1.187881823992999	2.758050746136902
9	0.9909314548810005	2.58836281624621
>10	9.106411199583665	49.641185999904444
>50	0.17864570705716304	3.36594273038962
>100	0.028008013394555217	1.2907064513438429
>500	7.569733349879788E-4	0.20321761148817952
>1k	0.0015139466699759577	0.49716914032189213
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1207	0.26661188642819594	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1056	0.2332577896173777	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	925	0.20432145397355528	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005963977575444316	0.0
2	0.0	0.0	0.0	0.02208880583497895	0.0
3	0.0	0.0	0.0	0.028715447585472634	0.0
4	0.0	0.0	0.0	0.04638649225345579	0.0
5	0.0	0.0	0.0	0.08018236518097359	0.0
6	0.0	0.0	0.0	0.12524352908433065	0.0
7	0.0	0.0	0.0	0.1555051930782518	0.0
8	0.0	0.0	0.0	0.4289646093152912	0.0
9	0.0	0.0	0.0	0.5528828100495231	0.0
10	0.0	0.0	0.0	0.7432883163470416	0.0
11	0.0	0.0	0.0	0.8665438529062242	0.0
12	0.0	0.0	0.0	0.992670934223954	0.0
13	0.0	0.0	0.0	1.0434751876444055	0.0
14	0.0	0.0	0.0	1.0593791278455904	0.0
15	0.0	0.0	0.0	1.0841185903807669	0.0
16	0.0	0.0	0.0	1.1486179034189055	0.0
17	0.0	0.0	0.0	1.2184185298574388	0.0
18	0.0	0.0	0.0	1.3597868872013041	0.0
19	0.0	0.0	0.0	1.4090449242133072	0.0
20	0.0	0.0	0.0	1.468022035792701	0.0
21	0.0	0.0	0.0	1.5358346697060863	0.0
22	0.0	0.0	0.0	1.6040890797361713	0.0
23	0.0	0.0	0.0	1.688468318025791	0.0
24	0.0	0.0	0.0	1.743027668438189	0.0
25	0.0	0.0	0.0	1.7894141606916447	0.0
26	0.0	0.0	0.0	1.8382304215869483	0.0
27	0.0	0.0	0.0	1.887709346657301	0.0
28	0.0	0.0	0.0	1.9345376150274565	0.0
29	0.0	0.0	0.0	1.9957236071903481	0.0
30	0.0	0.0	0.0	2.065303345570532	0.0
31	0.0	0.0	0.0	2.143718606284707	0.0
32	0.0	0.0	0.0	2.2102059118479938	0.0
33	0.0	0.0	0.0	2.278902097994778	0.0
34	0.0	0.0	0.0	2.346052067733114	0.0
35	0.0	0.0	0.0	2.4498694551575153	0.0
36	0.0	0.0	0.0	2.5176820890709006	0.0
37	0.0	0.0	0.0	2.5934466930848785	0.0
38	0.0	0.0	0.0	2.6647935359318606	0.0
39	0.0	0.0	0.0	2.736582154895542	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTAA	20	7.0287497E-4	45.000004	1
ACACGTG	20	7.0287497E-4	45.000004	42
CGCATCG	20	7.0287497E-4	45.000004	21
CACGACC	20	7.0287497E-4	45.000004	27
ATCGTAG	20	7.0287497E-4	45.000004	2
CGTTAAG	20	7.0287497E-4	45.000004	2
ACGGCCT	20	7.0287497E-4	45.000004	44
ACGTGAC	20	7.0287497E-4	45.000004	25
ACTCGAT	20	7.0287497E-4	45.000004	19
GGCATAT	20	7.0287497E-4	45.000004	8
GCGGACC	20	7.0287497E-4	45.000004	14
CGGTAGT	20	7.0287497E-4	45.000004	12
ACTCAAG	20	7.0287497E-4	45.000004	2
CTTACGT	20	7.0287497E-4	45.000004	22
AACACGT	20	7.0287497E-4	45.000004	41
TCACGAC	100	0.0	45.0	25
CGGGTAT	35	1.2097553E-7	45.0	6
GTTAGGC	55	1.8189894E-12	45.0	4
CACGACG	95	0.0	45.0	26
TGGATAA	25	3.8870465E-5	45.0	1
>>END_MODULE