FastQCFastQC Report
Sat 18 Jun 2016
SRR3549093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012129
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40230.39747897748211936No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23040.2276389669696254No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18520.18298062796343156No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17290.1708280268621885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGG650.044.0000042
CTTAACG302.5274167E-644.01
GGTAATC351.4458601E-744.08
GATCGAA454.802132E-1044.01
CAACGAC207.856036E-444.012
GCGCGTA302.5274167E-644.040
TAGCGAA502.7284841E-1144.01
CCGTGAT207.856036E-444.017
CATCTCG207.856036E-444.022
AAGGTAC1400.044.06
CGACATA207.856036E-444.027
CGACACT207.856036E-444.011
ACTTACG207.856036E-444.01
CGCCTAT302.5274167E-644.016
TCGGATT302.5274167E-644.044
AATGCGT302.5274167E-644.029
CGCTAAG454.802132E-1044.02
GGAACGT254.4424625E-544.08
AGACCGC207.856036E-444.023
GTCGTAA408.310963E-944.01
Produced by FastQC (version 0.11.5)