##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549093_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1012129
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55490752660975	31.0	31.0	33.0	30.0	34.0
2	31.74070202513711	31.0	31.0	34.0	30.0	34.0
3	31.763137900405976	31.0	31.0	34.0	30.0	34.0
4	35.6677933346441	37.0	35.0	37.0	35.0	37.0
5	27.139627458555186	35.0	26.0	37.0	0.0	37.0
6	31.357434674828998	35.0	26.0	37.0	17.0	37.0
7	14.934755352331571	0.0	0.0	32.0	0.0	37.0
8	25.080484799862468	17.0	17.0	35.0	17.0	37.0
9	33.24715920599054	32.0	32.0	37.0	28.0	39.0
10	36.11322766169135	37.0	35.0	39.0	32.0	39.0
11	37.078282511419	39.0	37.0	39.0	34.0	39.0
12	36.910741614952244	39.0	35.0	39.0	33.0	39.0
13	36.33604412085811	39.0	35.0	39.0	32.0	39.0
14	37.64881946866457	40.0	36.0	41.0	33.0	41.0
15	37.97748705945586	40.0	36.0	41.0	33.0	41.0
16	38.07394413162749	40.0	36.0	41.0	33.0	41.0
17	38.10833105266226	40.0	36.0	41.0	34.0	41.0
18	38.09257317990098	40.0	36.0	41.0	34.0	41.0
19	37.99979844466466	40.0	36.0	41.0	34.0	41.0
20	37.99446019232726	40.0	36.0	41.0	34.0	41.0
21	37.825437271335964	40.0	35.0	41.0	34.0	41.0
22	37.74376388780482	39.0	35.0	41.0	33.0	41.0
23	37.643516784915754	39.0	35.0	41.0	33.0	41.0
24	37.48916491870107	39.0	35.0	41.0	33.0	41.0
25	37.30204055016702	39.0	35.0	41.0	33.0	41.0
26	37.0738986828754	39.0	35.0	40.0	33.0	41.0
27	36.84133840646795	38.0	35.0	40.0	32.0	41.0
28	36.88406023342874	38.0	35.0	40.0	32.0	41.0
29	36.87366037333186	38.0	35.0	40.0	32.0	41.0
30	36.65874804496265	38.0	35.0	40.0	32.0	41.0
31	36.43101422842345	38.0	35.0	40.0	31.0	41.0
32	36.133746785241804	38.0	35.0	40.0	31.0	41.0
33	35.893364383393816	38.0	35.0	40.0	30.0	41.0
34	35.653772394625584	38.0	35.0	40.0	30.0	41.0
35	35.468579598055186	38.0	35.0	40.0	29.0	41.0
36	35.235345494497246	38.0	34.0	40.0	27.0	41.0
37	35.04423250395948	38.0	34.0	40.0	26.0	41.0
38	34.89843093123505	38.0	34.0	40.0	27.0	41.0
39	34.852654157721005	38.0	34.0	40.0	26.0	41.0
40	34.603604876453495	37.0	34.0	40.0	25.0	41.0
41	34.478080363273854	37.0	34.0	40.0	24.0	40.0
42	34.49099768902976	37.0	34.0	40.0	24.0	41.0
43	34.369570479652296	37.0	33.0	40.0	24.0	41.0
44	34.1081986584714	36.0	33.0	40.0	23.0	40.0
45	33.89248109677719	36.0	33.0	39.0	23.0	40.0
46	33.85972539073577	36.0	33.0	39.0	23.0	40.0
47	33.709811693963914	36.0	33.0	39.0	23.0	40.0
48	33.70231264986973	36.0	33.0	39.0	23.0	40.0
49	33.59705334003867	36.0	33.0	39.0	23.0	40.0
50	33.62789525841074	36.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	4.0
14	4.0
15	20.0
16	62.0
17	160.0
18	383.0
19	786.0
20	1518.0
21	2511.0
22	4114.0
23	6471.0
24	9844.0
25	14434.0
26	18231.0
27	18932.0
28	18682.0
29	20882.0
30	24826.0
31	33566.0
32	47341.0
33	92200.0
34	88637.0
35	105901.0
36	127209.0
37	240739.0
38	109946.0
39	24724.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.735410209568148	23.872846247859712	28.614237908408906	16.777505634163234
2	33.24625615904692	24.675905936891443	29.595041738750695	12.482796165310942
3	28.292045776773517	23.247431898503056	35.190968740150716	13.26955358457272
4	26.474293296605474	26.59463368799827	32.76104132971193	14.170031685684334
5	16.89073230783823	48.318544375272324	24.596074215836122	10.194649101053324
6	19.68879461017321	39.9899617538871	29.994496748932203	10.326746887007486
7	38.62985844689758	56.04246099064447	4.483222988374012	0.844457574083936
8	87.06617437105349	2.7244550842827344	8.653936405339635	1.5554341393241375
9	82.81661724938225	4.4648458842696925	10.394327205326594	2.3242096610214706
10	53.56066272184672	21.015799369447965	15.196778276286915	10.226759632418398
11	48.398178493057706	17.93378116821077	21.236818626874637	12.431221711856889
12	43.68020281999627	19.362749214774006	24.711375723845478	12.24567224138425
13	20.196042204106394	42.15806483165683	24.977349725183252	12.66854323905352
14	14.276935054721285	44.2493990390553	27.685897746235906	13.787768159987513
15	12.963169714532436	21.451514579663264	51.27824615241733	14.30706955338697
16	13.857719717545885	15.848572662180413	50.42045035761252	19.87325726266118
17	14.093855625122883	16.89784602555603	31.08546440226493	37.922833947056155
18	20.691334800208274	21.921711560482905	36.36413935377802	21.022814285530796
19	28.420389100598836	22.876332957557782	27.39601374923552	21.307264192607857
20	30.87827737373398	21.781314437191305	25.758179046346857	21.582229142727854
21	20.97726673180988	27.07619285683939	30.056939382232894	21.88960102911783
22	21.600112238657328	23.862373274552947	26.55580464545527	27.981709841334457
23	18.198174343389034	28.858376748418436	26.318186713353732	26.6252621948388
24	18.735260031082994	22.521733889652406	42.094436578736506	16.648569500528097
25	16.399589380405068	23.93617809587513	38.3768274597408	21.287405063978998
26	15.659762737753786	33.19043323528918	29.325016870379173	21.82478715657787
27	16.760906959488366	32.590015699579794	30.73560781283809	19.91346952809375
28	13.844776703364886	27.46537249698408	41.2039374427568	17.48591335689423
29	14.20747750533776	22.68406497590722	39.9165521391048	23.191905379650223
30	17.85740750437938	29.177802434274682	34.316574270671026	18.648215790674904
31	28.569777172672655	25.591401886518415	26.33695902399793	19.501861916811
32	29.910910565748043	24.67432511073193	27.3883072217079	18.02645710181212
33	27.087357441590942	27.215997170321177	25.635368614079823	20.061276774008054
34	18.492010405788196	27.234275472790525	29.240837877385196	25.032876244036085
35	19.740764270167144	25.003630960085125	31.628379386422083	23.62722538332564
36	31.600418523725732	23.65696467545145	26.37084798479245	18.371768816030368
37	20.28921214588259	31.838332860732176	29.30515774175031	18.56729725163492
38	20.489186655060767	31.730441475345533	25.538147805269883	22.242224064323814
39	20.665745176751184	31.40637211264572	27.858800607432453	20.069082103170643
40	23.79805341018783	25.820918084552464	26.341405097571553	24.03962340768815
41	17.607044161366783	23.506588587027938	28.165382080742674	30.72098517086261
42	22.523808723986765	25.452387986116392	24.41931809087577	27.604485199021074
43	22.26356521747722	26.269477507313795	25.898674971273422	25.568282303935565
44	19.57784037410251	30.112762305990636	28.81411361595212	21.495283703954733
45	17.276552692393953	37.30295248925779	22.78227380106686	22.638221017281392
46	22.498713108704525	31.725698996867003	26.065353329466895	19.71023456496158
47	21.606237940025434	27.454109110597564	27.610610900389183	23.329042048987827
48	22.79877367410676	23.585037085193687	30.744895166525215	22.87129407417434
49	20.760891151226772	23.65321021332261	32.343505620331	23.242393015119614
50	20.0592019396737	31.646756490526407	27.45776477109143	20.836276798708464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	823.0
1	1138.5
2	1454.0
3	6972.5
4	12491.0
5	9414.0
6	6337.0
7	6734.5
8	7132.0
9	7540.5
10	7949.0
11	7929.5
12	7910.0
13	7502.0
14	7094.0
15	6632.0
16	6170.0
17	5665.0
18	5160.0
19	4776.5
20	4393.0
21	4391.0
22	4389.0
23	4743.0
24	5097.0
25	5991.5
26	6886.0
27	8001.5
28	9117.0
29	11217.5
30	13318.0
31	15635.5
32	17953.0
33	20230.5
34	22508.0
35	23782.0
36	25056.0
37	30566.5
38	36077.0
39	50743.0
40	65409.0
41	81309.5
42	97210.0
43	102679.5
44	108149.0
45	103728.0
46	99307.0
47	92396.0
48	85485.0
49	80103.5
50	74722.0
51	67938.0
52	61154.0
53	54235.5
54	47317.0
55	42449.5
56	37582.0
57	34729.5
58	31877.0
59	29492.0
60	27107.0
61	24128.5
62	21150.0
63	18476.5
64	15803.0
65	13282.0
66	10761.0
67	9390.5
68	8020.0
69	6994.5
70	5969.0
71	4640.5
72	3312.0
73	2636.0
74	1960.0
75	1572.5
76	1185.0
77	952.0
78	719.0
79	574.0
80	429.0
81	282.5
82	136.0
83	85.5
84	35.0
85	22.0
86	9.0
87	7.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1012129.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.498628514987786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28554160335105	16.71322804568465
2	6.776348991697266	3.049171173042177
3	2.8148114139812406	1.899881890287343
4	1.7742749500980675	1.5967501194281963
5	1.306293340881214	1.469490430404437
6	0.9948799163669112	1.3430060193216753
7	0.8406226921288732	1.3239000370033527
8	0.7232402720174619	1.3017531365759671
9	0.6159925676916547	1.2473089153639205
>10	8.250639095907871	42.22375368427706
>50	1.4643496823423858	22.219175592711192
>100	0.1502486181569631	4.456116160186879
>500	9.189517930089486E-4	0.13221761631510737
>1k	0.0018379035860178973	1.0242471793980328
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4023	0.39747897748211936	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2304	0.2276389669696254	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1852	0.18298062796343156	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1729	0.1708280268621885	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007212519352770249	0.0
2	0.0	0.0	0.0	0.026676441441752977	0.0
3	0.0	0.0	0.0	0.04120028178226293	0.0
4	0.0	0.0	0.0	0.06827192976389374	0.0
5	0.0	0.0	0.0	0.12330444044188044	0.0
6	0.0	0.0	0.0	0.2334682634328233	0.0
7	0.0	0.0	0.0	0.31033593543905963	0.0
8	0.0	0.0	0.0	0.7661078775531578	0.0
9	0.0	0.0	0.0	0.9911780020135773	0.0
10	0.0	0.0	0.0	1.351408763112212	0.0
11	9.880163496945548E-5	0.0	0.0	1.6267689197720845	0.0
12	9.880163496945548E-5	0.0	0.0	1.8940273423644614	0.0
13	9.880163496945548E-5	0.0	0.0	2.0012271163063207	0.0
14	9.880163496945548E-5	0.0	0.0	2.0382777294198666	0.0
15	9.880163496945548E-5	0.0	0.0	2.0862953240150217	0.0
16	9.880163496945548E-5	0.0	0.0	2.2066357154078187	0.0
17	9.880163496945548E-5	0.0	0.0	2.3472304419693537	0.0
18	9.880163496945548E-5	0.0	0.0	2.5818843250218104	0.0
19	9.880163496945548E-5	0.0	0.0	2.6693237719697787	0.0
20	9.880163496945548E-5	0.0	0.0	2.7832420570895606	0.0
21	9.880163496945548E-5	0.0	0.0	2.9035824484823576	0.0
22	9.880163496945548E-5	0.0	0.0	3.022144410445704	0.0
23	9.880163496945548E-5	0.0	0.0	3.161158310847728	0.0
24	9.880163496945548E-5	0.0	0.0	3.2675676717098314	0.0
25	9.880163496945548E-5	0.0	0.0	3.343150922461465	0.0
26	9.880163496945548E-5	0.0	0.0	3.4242670647713878	0.0
27	9.880163496945548E-5	0.0	0.0	3.5054820087162804	0.0
28	9.880163496945548E-5	0.0	0.0	3.5853137297716002	0.0
29	9.880163496945548E-5	0.0	0.0	3.6829297451214225	0.0
30	9.880163496945548E-5	0.0	0.0	3.81394071309092	0.0
31	9.880163496945548E-5	0.0	0.0	3.931218253799664	0.0
32	9.880163496945548E-5	0.0	0.0	4.02982228549918	0.0
33	9.880163496945548E-5	0.0	0.0	4.121114996210958	0.0
34	9.880163496945548E-5	0.0	0.0	4.218335805020901	0.0
35	9.880163496945548E-5	0.0	0.0	4.36377181169594	0.0
36	9.880163496945548E-5	0.0	0.0	4.4759116673862716	0.0
37	9.880163496945548E-5	0.0	0.0	4.578862971024445	0.0
38	9.880163496945548E-5	0.0	0.0	4.676478986374266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGG	65	0.0	44.000004	2
CTTAACG	30	2.5274167E-6	44.0	1
GGTAATC	35	1.4458601E-7	44.0	8
GATCGAA	45	4.802132E-10	44.0	1
CAACGAC	20	7.856036E-4	44.0	12
GCGCGTA	30	2.5274167E-6	44.0	40
TAGCGAA	50	2.7284841E-11	44.0	1
CCGTGAT	20	7.856036E-4	44.0	17
CATCTCG	20	7.856036E-4	44.0	22
AAGGTAC	140	0.0	44.0	6
CGACATA	20	7.856036E-4	44.0	27
CGACACT	20	7.856036E-4	44.0	11
ACTTACG	20	7.856036E-4	44.0	1
CGCCTAT	30	2.5274167E-6	44.0	16
TCGGATT	30	2.5274167E-6	44.0	44
AATGCGT	30	2.5274167E-6	44.0	29
CGCTAAG	45	4.802132E-10	44.0	2
GGAACGT	25	4.4424625E-5	44.0	8
AGACCGC	20	7.856036E-4	44.0	23
GTCGTAA	40	8.310963E-9	44.0	1
>>END_MODULE