##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549087_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2593226
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99671875879696	31.0	31.0	34.0	31.0	34.0
2	32.65493481863902	34.0	31.0	34.0	31.0	34.0
3	32.611645109219175	34.0	31.0	34.0	31.0	34.0
4	36.059338060007114	37.0	35.0	37.0	35.0	37.0
5	35.835512986527206	37.0	35.0	37.0	35.0	37.0
6	35.833223174532414	37.0	35.0	37.0	35.0	37.0
7	35.81028764943742	37.0	35.0	37.0	35.0	37.0
8	35.77863903878798	37.0	35.0	37.0	33.0	37.0
9	37.96642753080526	39.0	38.0	39.0	35.0	39.0
10	37.928652959672625	39.0	39.0	39.0	35.0	39.0
11	37.74794599467999	39.0	38.0	39.0	35.0	39.0
12	37.27328663217167	39.0	37.0	39.0	34.0	39.0
13	37.02586083897046	39.0	37.0	39.0	33.0	39.0
14	37.78264254638817	40.0	36.0	41.0	33.0	41.0
15	37.676102661318374	40.0	36.0	41.0	33.0	41.0
16	37.73663537231232	40.0	36.0	41.0	33.0	41.0
17	37.827113795712364	40.0	36.0	41.0	33.0	41.0
18	37.886667031720336	40.0	36.0	41.0	33.0	41.0
19	37.827376017362155	40.0	36.0	41.0	33.0	41.0
20	37.76509259123578	40.0	36.0	41.0	33.0	41.0
21	37.77060464456241	40.0	36.0	41.0	33.0	41.0
22	37.68787255719324	40.0	35.0	41.0	33.0	41.0
23	37.62919120817082	40.0	35.0	41.0	33.0	41.0
24	37.58305253765002	40.0	35.0	41.0	33.0	41.0
25	37.527962468369516	40.0	35.0	41.0	33.0	41.0
26	37.455849972196795	40.0	35.0	41.0	32.0	41.0
27	37.37642264885513	39.0	35.0	41.0	32.0	41.0
28	37.26920522931669	39.0	35.0	41.0	32.0	41.0
29	37.208904661606816	39.0	35.0	41.0	32.0	41.0
30	37.140273929075214	39.0	35.0	41.0	32.0	41.0
31	37.054382070826065	39.0	35.0	41.0	31.0	41.0
32	36.97392706998927	39.0	35.0	41.0	31.0	41.0
33	36.86001991342058	39.0	35.0	41.0	31.0	41.0
34	36.76795929086011	39.0	35.0	41.0	31.0	41.0
35	36.71747044029329	39.0	35.0	41.0	31.0	41.0
36	36.625316497675094	39.0	35.0	41.0	31.0	41.0
37	36.45950487925079	38.0	35.0	41.0	30.0	41.0
38	36.389687593753884	38.0	35.0	41.0	30.0	41.0
39	36.33268754825071	38.0	35.0	41.0	30.0	41.0
40	36.23018896154828	38.0	35.0	41.0	30.0	41.0
41	36.22490789464551	38.0	35.0	40.0	30.0	41.0
42	36.05451279603089	38.0	35.0	40.0	30.0	41.0
43	35.94787303536213	38.0	35.0	40.0	30.0	41.0
44	35.87214419414274	38.0	35.0	40.0	30.0	41.0
45	35.859285692801166	38.0	35.0	40.0	30.0	41.0
46	35.79541582569356	38.0	35.0	40.0	30.0	41.0
47	35.72719577853994	37.0	35.0	40.0	29.0	41.0
48	35.68753976707005	37.0	35.0	40.0	29.0	41.0
49	35.653695050103615	37.0	35.0	40.0	29.0	41.0
50	35.54882682805124	37.0	34.0	40.0	29.0	41.0
51	35.37363692944618	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	21.0
11	24.0
12	19.0
13	28.0
14	43.0
15	68.0
16	107.0
17	247.0
18	508.0
19	999.0
20	1826.0
21	3012.0
22	4907.0
23	7509.0
24	11197.0
25	15816.0
26	22542.0
27	27914.0
28	33119.0
29	39744.0
30	48770.0
31	60555.0
32	76230.0
33	98462.0
34	160355.0
35	309835.0
36	189446.0
37	258948.0
38	392186.0
39	828166.0
40	609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.6556597843766798	91.9453992825924	3.241252401449006	3.157688531581898
2	3.0510260193288206	2.380934018091751	91.95253325394701	2.615506708632414
3	27.244559479196955	15.670828535576922	44.032066622808806	13.052545362417314
4	26.96024179921071	15.335724691947405	44.06125806235168	13.642775446490202
5	24.216632102254103	13.952081307221198	47.03839927565126	14.792887314873443
6	23.124440368868736	13.869905669617689	47.707064482617405	15.29858947889617
7	22.8806899205854	17.12484758366606	45.21588168559161	14.778580810156924
8	25.47282805278059	19.50770970212392	43.428764018253716	11.590698226841779
9	83.13232244316538	4.0664793581431	8.897450511447904	3.903747687243611
10	84.18074629824011	3.9469756974517454	8.53828397524936	3.3339940290587866
11	77.20646021596266	6.9703527575305815	9.818388370315583	6.004798656191169
12	42.11322113845843	25.83099197678876	15.314091405839674	16.741695478913137
13	33.275001870257356	23.355889536816303	25.50363909663099	17.865469496295347
14	29.258421749589118	23.37713720285081	27.36356183379312	20.000879213766947
15	21.399291847297537	27.83691047367256	31.175686191639297	19.58811148739061
16	16.97214203467033	32.89474191605359	30.677696429080996	19.455419620195077
17	17.47649452843678	24.182658973803285	39.48695562978313	18.853890867976798
18	20.411718839777173	21.303079639028763	36.63803309082972	21.647168430364342
19	20.201555899871433	21.18238055611042	29.718736430993676	28.897327113024474
20	21.78120996781615	22.928159751598972	32.27616875659892	23.014461523985954
21	25.675702773302444	24.732283264165947	27.902543010134867	21.689470952396743
22	27.606695289959305	22.54142138016509	27.7534237278201	22.09845960205551
23	25.22240637723052	24.86732741380813	28.330195671337556	21.580070537623794
24	27.99096569292456	21.195954382687816	26.011577857078404	24.80150206730921
25	25.538383465228254	24.70247483250592	24.637420726153447	25.12172097611238
26	25.418070002383132	21.17061914387716	31.48749858284623	21.923812270893475
27	25.6796360980493	23.40019728322946	27.52236017994575	23.397806438775486
28	21.782058331977236	27.218800058305753	26.944161442157377	24.054980167559634
29	21.730462366180195	26.446634423686945	27.937171692710162	23.885731517422702
30	22.934638168829096	26.27611322730838	30.16586290589405	20.623385697968477
31	21.175246584755822	24.481244596498726	30.65232262826302	23.691186190482433
32	22.64179828522466	26.63292748106027	28.482399914238094	22.242874319476975
33	25.921689818010464	27.424258433318194	25.166761400664655	21.48729034800669
34	25.139228127436635	25.597499022453114	27.453295624831775	21.809977225278477
35	25.562253347760667	26.847062307720194	23.830857781003274	23.75982656351587
36	23.46590694370641	26.284095562824067	27.065786013251447	23.184211480218075
37	22.63254340346734	25.69698900134427	28.36274200551745	23.307725589670937
38	25.985471378121304	27.318868467306746	24.876466609543478	21.81919354502847
39	24.971599081607234	27.161766849476287	26.575932834238124	21.290701234678348
40	22.233773685748947	27.334023336184348	26.97103916126092	23.461163816805787
41	23.157140951077924	27.851872532513553	24.950621349623983	24.04036516678454
42	24.867018917749554	24.79444521996926	27.346478864549407	22.992056997731783
43	21.546984335341385	25.163290820005663	28.46138362024752	24.82834122440543
44	22.27680888592047	25.53849915125022	26.25980149821111	25.9248904646182
45	22.57068994372261	22.970462273631377	28.454789516995433	26.00405826565058
46	22.741905256232968	24.566852252753907	27.972841549483153	24.71840094152997
47	20.129714880230264	26.97767182652033	27.99964214457205	24.89297114867736
48	21.515440613351863	27.13909238917086	26.268902131939136	25.07656486553814
49	22.255252723827386	25.224180229567345	29.523304177884995	22.997262868720274
50	22.56054813579688	22.851228546991276	30.93968670682771	23.648536610384134
51	21.204129528240117	24.199240636951814	28.093733442438108	26.50289639236997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1013.0
1	2119.0
2	3225.0
3	7435.5
4	11646.0
5	8023.5
6	4401.0
7	4460.0
8	4519.0
9	4904.0
10	5289.0
11	5441.0
12	5593.0
13	6150.0
14	6707.0
15	6233.5
16	5760.0
17	6009.0
18	6258.0
19	6287.5
20	6317.0
21	7440.0
22	8563.0
23	11780.0
24	14997.0
25	17549.5
26	25150.5
27	30199.0
28	32718.0
29	35237.0
30	40151.5
31	45066.0
32	51390.0
33	57714.0
34	68647.5
35	79581.0
36	91317.5
37	103054.0
38	115491.0
39	127928.0
40	156553.0
41	185178.0
42	205968.5
43	226759.0
44	243509.5
45	260260.0
46	247112.0
47	233964.0
48	210378.0
49	186792.0
50	163373.0
51	139954.0
52	137417.0
53	134880.0
54	133219.0
55	131558.0
56	131274.5
57	130991.0
58	123920.5
59	116850.0
60	103532.5
61	90215.0
62	74997.0
63	59779.0
64	49976.0
65	40173.0
66	33159.0
67	26145.0
68	23169.5
69	20194.0
70	16043.0
71	11892.0
72	9526.0
73	7160.0
74	5681.5
75	3244.0
76	2285.0
77	1309.0
78	333.0
79	376.5
80	420.0
81	229.5
82	39.0
83	28.0
84	17.0
85	12.0
86	7.0
87	5.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	1.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2593226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.672723090881709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.73152671413501	9.534198554667887
2	11.701854872521942	3.1999244264335482
3	5.178233754267703	2.124016686658773
4	2.9258993994489693	1.600200491217714
5	1.887801394551465	1.2905692859141251
6	1.2427128706755246	1.0194761377332684
7	0.8720514964772804	0.8346323042626003
8	0.6262835782721665	0.6850401553665498
9	0.4855202357213869	0.5974545364234323
>10	2.879241556076634	7.667300852844939
>50	0.5942633525300299	5.953635605583641
>100	1.8172477986347808	58.28865725146849
>500	0.050015994451373696	4.156214734466812
>1k	0.006499253516280197	2.0862208992600855
>5k	5.65152479676539E-4	0.5143206587578988
>10k+	2.825762398382695E-4	0.44813741894026954
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	11599	0.4472807229296637	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6894	0.26584647847892934	No Hit
ATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	6418	0.24749096299358403	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	4797	0.1849819491243725	No Hit
ATGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	4501	0.17356759495701493	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	4392	0.16936433615890015	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	3702	0.14275655110661392	No Hit
ATTGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	3492	0.1346585295689616	No Hit
ATGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	3294	0.12702325211917512	No Hit
ATATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	3075	0.1185781725156234	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.856200732215395E-5	0.0	0.0	0.20110086818503284	0.0
2	3.856200732215395E-5	0.0	0.0	0.854881140324831	0.0
3	3.856200732215395E-5	0.0	0.0	1.2029803804219146	0.0
4	3.856200732215395E-5	0.0	0.0	1.580579556120446	0.0
5	3.856200732215395E-5	0.0	0.0	2.884630957733726	0.0
6	3.856200732215395E-5	0.0	0.0	3.2002995496728786	0.0
7	3.856200732215395E-5	0.0	0.0	3.7216193266610778	0.0
8	3.856200732215395E-5	0.0	0.0	5.225730422261693	0.0
9	7.71240146443079E-5	0.0	0.0	5.414684258140247	0.0
10	7.71240146443079E-5	0.0	0.0	5.779557971422467	0.0
11	7.71240146443079E-5	0.0	0.0	6.0648397015917626	0.0
12	7.71240146443079E-5	0.0	0.0	6.856324901878972	0.0
13	7.71240146443079E-5	0.0	0.0	7.574118106173546	0.0
14	7.71240146443079E-5	0.0	0.0	7.887318729644081	0.0
15	7.71240146443079E-5	0.0	0.0	8.05113013674859	0.0
16	7.71240146443079E-5	0.0	0.0	8.159065195243299	0.0
17	7.71240146443079E-5	0.0	0.0	8.320370071871869	0.0
18	7.71240146443079E-5	0.0	0.0	8.651540590754527	0.0
19	7.71240146443079E-5	0.0	0.0	9.098474255618292	0.0
20	7.71240146443079E-5	0.0	0.0	9.603983609604407	0.0
21	1.1568602196646186E-4	0.0	0.0	9.917261357089586	0.0
22	0.0011568602196646185	0.0	0.0	10.256182839443998	0.0
23	0.0011954222269867725	0.0	0.0	10.772720927524249	0.0
24	0.0034320186516717016	0.0	0.0	11.200257902704971	0.0
25	0.0034705806589938556	0.0	0.0	11.669596093822907	0.0
26	0.004357506827403396	0.0	0.0	12.03470117914906	0.0
27	0.004357506827403396	0.0	0.0	12.36571745000243	0.0
28	0.004511754856692012	0.0	0.0	12.666732479159164	0.0
29	0.004511754856692012	0.0	0.0	12.960343602910044	0.0
30	0.004511754856692012	0.0	0.0	13.248941665709044	0.0
31	0.004550316864014166	0.0	0.0	13.57741284407915	0.0
32	0.004550316864014166	0.0	0.0	13.928519920747362	0.0
33	0.00458887887133632	0.0	0.0	14.269485189489847	0.0
34	0.00458887887133632	0.0	0.0	14.616157635316013	0.0
35	0.00458887887133632	0.0	0.0	14.945207243795952	0.0
36	0.00458887887133632	0.0	0.0	15.281313699615845	0.0
37	0.004627440878658474	0.0	0.0	15.650429233703504	0.0
38	0.004627440878658474	0.0	0.0	15.993978156936572	0.0
39	0.004627440878658474	0.0	0.0	16.359931606423814	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGCG	30	2.16656E-6	45.000004	3
ACCGAAC	30	2.16656E-6	45.000004	13
CTAACCG	60	0.0	45.000004	23
GTTCGAA	60	0.0	45.000004	39
TCTAACG	30	2.16656E-6	45.000004	41
CGTAATC	30	2.16656E-6	45.000004	36
ACCGTAT	30	2.16656E-6	45.000004	15
ACACTCG	30	2.16656E-6	45.000004	33
CGTCCTA	30	2.16656E-6	45.000004	23
TCGACGA	30	2.16656E-6	45.000004	30
GTACGAC	30	2.16656E-6	45.000004	11
CTTACGT	30	2.16656E-6	45.000004	22
AACCGTA	45	3.8562575E-10	45.0	38
CGAGTTA	25	3.8921666E-5	45.0	17
CGTATAC	35	1.2127202E-7	45.0	19
GTCGAAC	20	7.034929E-4	45.0	17
TCCGATT	20	7.034929E-4	45.0	32
ACGTTCA	45	3.8562575E-10	45.0	19
GCGTTTC	40	6.8212103E-9	45.0	27
CGACACG	50	2.1827873E-11	45.0	3
>>END_MODULE