##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549083_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1569551
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01352233855415	31.0	31.0	34.0	31.0	34.0
2	32.689841234849965	34.0	31.0	34.0	31.0	34.0
3	32.61489050053168	34.0	31.0	34.0	31.0	34.0
4	36.052229586677974	37.0	35.0	37.0	35.0	37.0
5	35.80774947739831	37.0	35.0	37.0	35.0	37.0
6	35.78849620050575	37.0	35.0	37.0	35.0	37.0
7	35.76077362252007	37.0	35.0	37.0	33.0	37.0
8	35.7393592180184	37.0	35.0	37.0	33.0	37.0
9	37.978698366602934	39.0	38.0	39.0	35.0	39.0
10	37.98520659730076	39.0	39.0	39.0	35.0	39.0
11	37.783385184680206	39.0	38.0	39.0	35.0	39.0
12	37.22125627010527	39.0	37.0	39.0	33.0	39.0
13	36.95871430746755	39.0	37.0	39.0	33.0	39.0
14	37.7722189339499	40.0	36.0	41.0	33.0	41.0
15	37.66244040493109	40.0	36.0	41.0	33.0	41.0
16	37.73415900470899	40.0	36.0	41.0	33.0	41.0
17	37.839911541581	40.0	36.0	41.0	33.0	41.0
18	37.90071491783319	40.0	36.0	41.0	33.0	41.0
19	37.884072578718374	40.0	36.0	41.0	33.0	41.0
20	37.84661473249356	40.0	36.0	41.0	33.0	41.0
21	37.806742820080395	40.0	36.0	41.0	33.0	41.0
22	37.771853224266046	40.0	35.0	41.0	33.0	41.0
23	37.71433167829526	40.0	35.0	41.0	33.0	41.0
24	37.68478437463963	40.0	35.0	41.0	33.0	41.0
25	37.658605550249725	40.0	35.0	41.0	33.0	41.0
26	37.539807244237366	40.0	35.0	41.0	33.0	41.0
27	37.44163330786957	40.0	35.0	41.0	32.0	41.0
28	37.36541788065504	39.0	35.0	41.0	32.0	41.0
29	37.3309252136439	39.0	35.0	41.0	32.0	41.0
30	37.30223611720804	39.0	35.0	41.0	32.0	41.0
31	37.273750263610424	39.0	35.0	41.0	32.0	41.0
32	37.17410138313441	39.0	35.0	41.0	32.0	41.0
33	37.09980242757324	39.0	35.0	41.0	32.0	41.0
34	37.033982967103334	39.0	35.0	41.0	31.0	41.0
35	36.90876307937748	39.0	35.0	41.0	31.0	41.0
36	36.88174325013969	39.0	35.0	41.0	31.0	41.0
37	36.73776067168254	39.0	35.0	41.0	31.0	41.0
38	36.606941730469416	39.0	35.0	41.0	30.0	41.0
39	36.612309507623515	39.0	35.0	41.0	30.0	41.0
40	36.559448530184746	39.0	35.0	41.0	30.0	41.0
41	36.55410305240161	39.0	35.0	41.0	30.0	41.0
42	36.43429872619622	39.0	35.0	41.0	30.0	41.0
43	36.36420033500026	38.0	35.0	41.0	30.0	41.0
44	36.28868192240966	38.0	35.0	40.0	30.0	41.0
45	36.30381746117202	38.0	35.0	40.0	30.0	41.0
46	36.26002468221804	38.0	35.0	40.0	30.0	41.0
47	36.18259170934873	38.0	35.0	40.0	30.0	41.0
48	36.11092344243672	38.0	35.0	40.0	30.0	41.0
49	36.113779036170214	38.0	35.0	40.0	30.0	41.0
50	36.062610262425366	38.0	35.0	40.0	30.0	41.0
51	35.90481481646662	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	7.0
11	7.0
12	8.0
13	9.0
14	28.0
15	27.0
16	61.0
17	146.0
18	274.0
19	544.0
20	971.0
21	1665.0
22	2640.0
23	4148.0
24	6429.0
25	9678.0
26	13213.0
27	16100.0
28	18939.0
29	22536.0
30	28224.0
31	35502.0
32	44974.0
33	58114.0
34	89274.0
35	183811.0
36	104564.0
37	139046.0
38	222460.0
39	565716.0
40	430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.276479706616733	92.79207875373275	2.8247568890720975	3.106684650578414
2	3.1266266594714027	1.8872276211477041	92.53945873692541	2.4466869824554918
3	26.05025258816056	15.593376704547989	45.2098084101759	13.146562297115544
4	26.45317036528281	15.989413532914828	45.45338125361967	12.104034848182698
5	23.264105467104923	13.163637244027113	48.48552229268115	15.086734996186808
6	20.836149956261377	14.583788612157234	49.720652594276956	14.859408837304427
7	21.27786863886551	17.050863590925047	47.49148004747855	14.179787722730897
8	23.466392618016236	19.508317983932987	44.95170911935961	12.073580278691166
9	83.39493269094154	3.356119042961968	9.05271635008993	4.196231916006552
10	85.70591207294316	3.2065858325087877	8.22209663782827	2.865405456719788
11	78.26817988074296	6.732371232282354	8.66642753245992	6.333021354514763
12	36.52732533062003	33.096662676141136	15.217027035120234	15.158984958118596
13	28.514333079970005	25.06946254056096	29.847198338888	16.569006040581034
14	28.73898331433639	25.14617237668607	28.459986327299973	17.654857981677562
15	19.400070466012252	34.89596706319196	30.72999857921151	14.97396389158428
16	14.14958800319327	38.71928978414846	27.955192281104598	19.17592993155367
17	12.639729451288936	27.274997754134784	39.904533207267555	20.18073958730873
18	15.762533361451778	24.080772144390338	38.29318066122095	21.863513832936935
19	15.902573411122034	23.797251570672124	31.66268569801172	28.63748932019412
20	18.68604460766168	24.21405867028214	35.29168532911642	21.808211392939764
21	20.277136582372922	30.126641313343754	29.360562351908285	20.235659752375042
22	24.78364831725761	23.76329281431441	29.62133756724057	21.83172130118741
23	18.916174116037006	28.829901035391654	29.85465270003969	22.39927214853165
24	25.10310273447629	23.52239589538664	26.015019582033332	25.359481788103732
25	20.83098924469482	28.5172638544399	25.428100138192388	25.223646762672892
26	19.455054343567046	24.06000187314716	37.418790469376276	19.06615331390952
27	21.090299072792153	29.14616982818653	29.521117822867815	20.2424132761535
28	19.21441227459318	31.670649759071225	27.127630768289784	21.987307198045812
29	16.155894265302624	32.23278504489501	26.82939260973361	24.781928080068756
30	17.610195527255883	26.69623350881876	33.22746441498237	22.466106548942978
31	17.346362112476754	22.803973875331227	32.854682644909275	26.994981367282744
32	15.801461691910617	32.402706251660504	31.186944546561406	20.608887509867472
33	20.275034070253213	30.731527678934935	29.6884268176058	19.305011433206058
34	24.238333128391496	26.233999404925356	26.557977408825835	22.96969005785731
35	21.178094881912088	29.391845183750004	26.979499232583077	22.45056070175483
36	19.372674095967575	25.639243325001864	29.674282645164123	25.313799933866438
37	18.148502342389637	27.16611311132929	33.44434172575469	21.241042820526378
38	21.36521846056611	28.768737046454685	29.128776318832582	20.73726817414662
39	21.978642299613075	29.721684736590277	28.550458060935895	19.749214902860754
40	17.476717863898656	27.523603884168146	34.379577344093946	20.620100907839248
41	18.751859608257394	31.654721636952228	27.151268101514383	22.442150653276
42	22.72834715151021	27.217847651971805	29.859112574233016	20.194692622284972
43	18.956249271288414	27.09953356087187	30.74076598976395	23.203451178075767
44	18.049875410228786	28.9123449954796	26.95273998742315	26.08503960686846
45	18.916811240921767	25.347567552758722	29.603689207932714	26.131931998386797
46	20.766384781380154	26.718724017250793	29.166557824498852	23.3483333768702
47	16.6272392550481	31.758127005748776	27.541507093429907	24.07312664577322
48	17.977179460877664	30.40920619973483	28.47393936227622	23.139674977111287
49	18.68700029498882	28.595120515357575	30.93719159173547	21.78068759791813
50	18.80531438608876	24.36225391847732	33.917406952689014	22.9150247427449
51	17.949145965948222	24.950192762133884	31.07270805472393	26.02795321719396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1102.0
1	1115.0
2	1128.0
3	4259.5
4	7391.0
5	4897.5
6	2404.0
7	2619.5
8	2835.0
9	3029.0
10	3223.0
11	3474.5
12	3726.0
13	3763.0
14	3800.0
15	3741.0
16	3682.0
17	3893.5
18	4105.0
19	4936.5
20	5768.0
21	5219.5
22	4671.0
23	6584.5
24	8498.0
25	12965.0
26	23757.5
27	30083.0
28	33265.5
29	36448.0
30	39364.0
31	42280.0
32	52609.0
33	62938.0
34	74484.5
35	86031.0
36	89712.5
37	93394.0
38	102443.0
39	111492.0
40	124462.5
41	137433.0
42	144018.5
43	150604.0
44	158285.0
45	165966.0
46	170803.0
47	175640.0
48	158619.0
49	141598.0
50	114119.5
51	86641.0
52	72116.5
53	57592.0
54	47920.5
55	38249.0
56	32320.0
57	26391.0
58	23408.5
59	20426.0
60	17625.0
61	14824.0
62	11668.5
63	8513.0
64	7321.0
65	6129.0
66	4595.0
67	3061.0
68	2418.5
69	1776.0
70	1313.0
71	850.0
72	743.5
73	637.0
74	520.0
75	314.0
76	225.0
77	158.5
78	92.0
79	72.0
80	52.0
81	33.0
82	14.0
83	8.5
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1569551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.412629222205446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.00849727393049	10.63602381664666
2	12.2725366394002	3.7830411367801307
3	5.456241593015081	2.5228508585975113
4	3.032242641701194	1.8693932619320508
5	1.9164103394344305	1.476846099965188
6	1.2631144328219084	1.1680748650980244
7	0.8901363847668752	0.9603539438924378
8	0.6257354188156122	0.7715382401125175
9	0.48962453031215303	0.6791761209417935
>10	2.774654661025399	8.421487179829027
>50	0.5853448967883343	6.5182770110991255
>100	1.6559863741097276	55.224408546649265
>500	0.019096358287552635	1.9569419284640706
>1k	0.009548179143776318	3.082927348019758
>5k	8.302764472848971E-4	0.9286596419724544
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	9434	0.6010636162826184	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5080	0.3236594414580985	No Hit
ATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	4304	0.2742185504007197	No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	3644	0.23216830800655727	TruSeq Adapter, Index 15 (95% over 22bp)
ATGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT	3182	0.2027331383306436	No Hit
ATTGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	3001	0.19120117791648694	No Hit
ATATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	2763	0.17603760565919807	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2725	0.17361653109710995	No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	2558	0.16297654552161733	TruSeq Adapter, Index 14 (95% over 21bp)
ATGTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	2546	0.16221199565990527	No Hit
ATGCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	2494	0.1588989462591531	No Hit
ATGGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	2307	0.1469847109141404	No Hit
ATTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	2058	0.13112030128361551	No Hit
ATAGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	1908	0.12156342801221495	No Hit
ATAGCATTCCCACGAATAAATAATATAAGTTTTTGACTCCTACCACCATCA	1767	0.11257996713709845	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25370312911144655	0.0
2	0.0	0.0	0.0	1.1052205375932354	0.0
3	0.0	0.0	0.0	1.501193653471598	0.0
4	0.0	0.0	0.0	1.9459705355225794	0.0
5	0.0	0.0	0.0	3.7317041625280094	0.0
6	0.0	0.0	0.0	4.154563948543246	0.0
7	0.0	0.0	0.0	4.760915701369372	0.0
8	0.0	0.0	0.0	6.715487422836213	0.0
9	0.0	0.0	0.0	6.949758242962478	0.0
10	0.0	0.0	0.0	7.405429960542856	0.0
11	0.0	0.0	0.0	7.76183762107762	0.0
12	0.0	0.0	0.0	8.73944204425342	0.0
13	0.0	0.0	0.0	9.655755053515305	0.0
14	0.0	0.0	0.0	10.030766760685061	0.0
15	0.0	0.0	0.0	10.217444351919752	0.0
16	0.0	0.0	0.0	10.321869120531924	0.0
17	0.0	0.0	0.0	10.488795840339053	0.0
18	0.0	0.0	0.0	10.93682205930231	0.0
19	0.0	0.0	0.0	11.529921614525428	0.0
20	6.371248847600365E-5	0.0	0.0	12.179151872095906	0.0
21	6.371248847600365E-5	0.0	0.0	12.561108240509547	0.0
22	6.371248847600365E-5	0.0	0.0	12.955615969152962	0.0
23	1.274249769520073E-4	0.0	0.0	13.626635897782233	0.0
24	1.9113746542801093E-4	0.0	0.0	14.17876832291528	0.0
25	3.1856244238001824E-4	0.0	0.0	14.791300187123579	0.0
26	0.0026122120275161496	0.0	0.0	15.269780975578366	0.0
27	0.0026122120275161496	0.0	0.0	15.68104508869097	0.0
28	0.0026122120275161496	0.0	0.0	16.031654912774417	0.0
29	0.0026122120275161496	0.0	0.0	16.376658037871977	0.0
30	0.0026122120275161496	0.0	0.0	16.701910291541974	0.0
31	0.0026122120275161496	0.0	0.0	17.069276500094613	0.0
32	0.0026122120275161496	0.0	0.0	17.538901252651236	0.0
33	0.002675924515992153	0.0	0.0	17.94551435410509	0.0
34	0.002675924515992153	0.0	0.0	18.318168699201237	0.0
35	0.002675924515992153	0.0	0.0	18.68833825724682	0.0
36	0.002675924515992153	0.0	0.0	19.048950942020998	0.0
37	0.002675924515992153	0.0	0.0	19.58827715697037	0.0
38	0.002739637004468157	0.0	0.0	19.973291724830858	0.0
39	0.002739637004468157	0.0	0.0	20.36550580388914	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTGA	40	6.8175723E-9	45.000004	18
CTTAACG	40	6.8175723E-9	45.000004	3
CTAGACG	20	7.0340745E-4	45.000004	28
TTAGGCG	35	1.2123019E-7	45.000004	6
AGATCGC	35	1.2123019E-7	45.000004	22
CGAACCG	20	7.0340745E-4	45.000004	3
GATCGCC	35	1.2123019E-7	45.000004	23
CGCGGTC	20	7.0340745E-4	45.000004	43
CGAGTAG	75	0.0	45.000004	3
CGTGCAT	20	7.0340745E-4	45.000004	43
GCAACGC	35	1.2123019E-7	45.000004	11
GACGTGC	20	7.0340745E-4	45.000004	41
GACGTAC	35	1.2123019E-7	45.000004	29
CGGCACG	20	7.0340745E-4	45.000004	33
CCGGATA	75	0.0	45.000004	22
CGCGCAC	20	7.0340745E-4	45.000004	27
GCCGGAC	20	7.0340745E-4	45.000004	25
CGCGATG	35	1.2123019E-7	45.000004	3
CACGACA	20	7.0340745E-4	45.000004	25
ACGGGTC	40	6.8175723E-9	45.000004	7
>>END_MODULE