##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549080_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1461268
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63818546632103	33.0	31.0	34.0	31.0	34.0
2	33.036312982970955	34.0	33.0	34.0	31.0	34.0
3	33.08146486476129	34.0	33.0	34.0	31.0	34.0
4	36.43752412288506	37.0	37.0	37.0	35.0	37.0
5	36.41773240774451	37.0	37.0	37.0	35.0	37.0
6	36.42729944130714	37.0	37.0	37.0	35.0	37.0
7	36.51907179244328	37.0	37.0	37.0	35.0	37.0
8	36.575281878478144	37.0	37.0	37.0	35.0	37.0
9	38.38217219565473	39.0	39.0	39.0	37.0	39.0
10	38.133105631547394	39.0	39.0	39.0	37.0	39.0
11	37.94190045905337	39.0	38.0	39.0	35.0	39.0
12	37.54063799385192	39.0	37.0	39.0	35.0	39.0
13	37.46396280490642	39.0	37.0	39.0	35.0	39.0
14	38.79488225294744	41.0	38.0	41.0	35.0	41.0
15	38.84401492402489	41.0	38.0	41.0	35.0	41.0
16	38.92519647319999	41.0	38.0	41.0	35.0	41.0
17	38.870435813279975	41.0	38.0	41.0	35.0	41.0
18	38.86794824768626	41.0	38.0	41.0	35.0	41.0
19	38.87226094049825	41.0	38.0	41.0	35.0	41.0
20	38.80062794778234	40.0	38.0	41.0	35.0	41.0
21	38.76942833210609	40.0	37.0	41.0	35.0	41.0
22	38.8426010834426	41.0	38.0	41.0	35.0	41.0
23	38.857550428805666	41.0	38.0	41.0	35.0	41.0
24	38.81775074798052	41.0	37.0	41.0	35.0	41.0
25	38.764781682757715	41.0	37.0	41.0	35.0	41.0
26	38.66401098224282	40.0	37.0	41.0	35.0	41.0
27	38.59404229751148	40.0	37.0	41.0	35.0	41.0
28	38.609241425939665	40.0	37.0	41.0	35.0	41.0
29	38.5722208383404	40.0	36.0	41.0	35.0	41.0
30	38.53365433308606	40.0	36.0	41.0	35.0	41.0
31	38.49252635382421	40.0	36.0	41.0	35.0	41.0
32	38.43572294746754	40.0	36.0	41.0	35.0	41.0
33	38.32081178811826	40.0	36.0	41.0	35.0	41.0
34	38.30710862073213	40.0	36.0	41.0	35.0	41.0
35	38.26153860893416	40.0	36.0	41.0	35.0	41.0
36	38.13664160167745	40.0	36.0	41.0	34.0	41.0
37	38.05373826019594	40.0	35.0	41.0	34.0	41.0
38	37.982785498621745	40.0	35.0	41.0	34.0	41.0
39	37.909220622089855	40.0	35.0	41.0	34.0	41.0
40	37.76762099765409	40.0	35.0	41.0	34.0	41.0
41	37.63874251677311	40.0	35.0	41.0	34.0	41.0
42	37.61651182397753	40.0	35.0	41.0	34.0	41.0
43	37.62785950284274	40.0	35.0	41.0	34.0	41.0
44	37.48031161977132	40.0	35.0	41.0	33.0	41.0
45	37.290847401024315	39.0	35.0	41.0	33.0	41.0
46	37.33009892778053	39.0	35.0	41.0	33.0	41.0
47	37.282516280381145	39.0	35.0	41.0	33.0	41.0
48	37.2030633668841	39.0	35.0	41.0	33.0	41.0
49	37.16108201917786	39.0	35.0	41.0	33.0	41.0
50	37.0549994935905	39.0	35.0	41.0	33.0	41.0
51	36.9174764656449	39.0	35.0	41.0	33.0	41.0
52	36.506845424658586	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	2.0
13	8.0
14	10.0
15	25.0
16	24.0
17	71.0
18	126.0
19	221.0
20	400.0
21	693.0
22	1148.0
23	1785.0
24	2674.0
25	3512.0
26	4537.0
27	5961.0
28	7405.0
29	9805.0
30	12586.0
31	16480.0
32	21957.0
33	31069.0
34	71656.0
35	186121.0
36	70918.0
37	94767.0
38	177129.0
39	738799.0
40	1374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.790866562464927	14.376692023639743	48.240637583249615	12.591803830645713
2	24.439596295819797	12.809149314157295	51.008986715646955	11.742267674375952
3	24.85943714636877	13.824637232868989	46.3757503757011	14.940175245061138
4	21.58727899331266	13.838392409879638	50.099707924898105	14.474620671909602
5	23.285324800105116	16.076585540776914	46.283570159614804	14.354519499503171
6	23.470095834576547	18.477514049441993	45.31879162480804	12.73359849117342
7	84.4031348116841	2.3312629852977005	7.6601280531702605	5.60547414984794
8	86.79249802226559	2.3116909423870227	7.4342967888162885	3.4615142465310953
9	78.06877314770459	4.5394821483807215	9.58489476263081	7.806849941283871
10	38.98025550412382	29.57835249933619	13.958425148569598	17.482966847970395
11	32.240424070054225	21.224648729733357	30.687936778195375	15.846990422017043
12	29.782353408135947	26.097471511043835	25.879578557800485	18.240596523019732
13	19.937478956632184	31.447551031022375	32.32206549380401	16.29290451854143
14	14.078868489558383	39.71653385963423	29.49917468937936	16.70542296142802
15	13.262727986926423	25.728271610683322	41.06173542430273	19.947264978087524
16	17.10001177059923	24.49222182378592	38.50867876392284	19.89908764169201
17	16.874727975977027	24.658105152511382	28.01320496993023	30.453961901581366
18	18.078750783566054	25.89134915703348	32.91750726081732	23.112392798583148
19	22.511818502834522	27.41906344352987	29.41979157827312	20.649326475362493
20	24.615881549448833	23.573225445298192	27.156962309446314	24.653930695806654
21	19.686737819482804	29.255892827325308	31.148358822611595	19.90901053058029
22	25.195515127957364	21.929721310533044	26.71823375315137	26.15652980835822
23	22.00842008447458	27.644826274167368	24.05787302534511	26.288880616012943
24	21.012025172658266	22.21522677564964	33.681569705214926	23.09117834647717
25	24.532529282787277	26.951387425167734	27.604587248882478	20.911496043162515
26	18.68083062107704	31.646214109937397	26.042861405300055	23.630093863685513
27	17.747394728413955	31.60399050687485	26.129087888053387	24.519526876657807
28	18.62485184100384	26.496508511785656	34.318687605559006	20.559952041651496
29	16.883624359118247	23.776268282067356	33.26754572056597	26.072561638248427
30	21.179345609429618	30.772315550603995	27.90165801208266	20.146680827883728
31	21.036182274572496	27.211298680324212	28.039551950771525	23.712967094331773
32	26.177333658165374	24.792235236794347	27.799212738525718	21.23121836651456
33	24.16271347897853	26.447099368493664	27.591379541603594	21.79880761092421
34	17.34356736751917	24.426936058272677	27.628196881064937	30.601299693143215
35	20.746639220184115	24.033031586266173	32.14646457733968	23.07386461621003
36	21.40654554811301	24.367535592375937	31.076708721466563	23.14921013804449
37	22.518867175631026	27.10331027573313	28.598860715488193	21.77896183314765
38	19.545559062403335	25.46042204441622	32.67449913362915	22.319519759551294
39	20.64309900716364	28.811552706279752	25.995026237486897	24.550322049069713
40	25.11407900535699	23.705028783221145	29.78755437058774	21.393337840834125
41	18.873129364360267	24.34118861153464	30.578579699274876	26.207102324830217
42	18.426941532969995	26.42910130106182	27.035218727844583	28.108738438123602
43	19.2853056386645	23.90143354949263	30.490094903878003	26.323165907964864
44	25.288584982357786	23.660889036097416	26.59566896695199	24.454857014592807
45	18.056988861728307	27.565032560762297	28.30596440899274	26.07201416851666
46	19.575533030217592	27.07142016385769	27.298962271123433	26.05408453480128
47	20.48481182096645	24.592135049833434	32.68250587845625	22.240547250743877
48	19.60413832370243	20.92251387151433	36.856894149464715	22.61645365531853
49	19.115863756682554	21.296161963445446	29.772704254113552	29.81527002575845
50	18.130760408083937	24.25537273108013	34.009298773394065	23.604568087441866
51	20.645083584941297	23.28429829435805	30.307513748333637	25.763104372367014
52	19.167736513767494	21.377392784896404	31.830437674677057	27.62443302665904
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	768.0
1	580.0
2	392.0
3	307.0
4	222.0
5	310.5
6	399.0
7	461.0
8	523.0
9	765.5
10	1008.0
11	1246.0
12	1484.0
13	1570.5
14	1576.0
15	1495.0
16	1994.0
17	2493.0
18	2104.5
19	1716.0
20	2313.5
21	2911.0
22	3625.0
23	4339.0
24	5268.0
25	6197.0
26	8539.5
27	10882.0
28	13550.5
29	16219.0
30	21828.5
31	27438.0
32	32096.5
33	36755.0
34	39639.0
35	42523.0
36	48343.5
37	54164.0
38	57663.0
39	77226.0
40	93290.0
41	103481.0
42	113672.0
43	116680.0
44	119688.0
45	133312.5
46	146937.0
47	163489.5
48	180042.0
49	174298.0
50	168554.0
51	139931.0
52	111308.0
53	89613.0
54	67918.0
55	60237.5
56	52557.0
57	47609.5
58	42662.0
59	37146.5
60	31631.0
61	27431.5
62	23232.0
63	18198.0
64	10380.5
65	7597.0
66	6540.0
67	5483.0
68	4485.5
69	3488.0
70	2587.0
71	1686.0
72	1634.5
73	1583.0
74	1244.5
75	906.0
76	674.5
77	443.0
78	383.5
79	324.0
80	309.0
81	294.0
82	159.5
83	25.0
84	20.0
85	15.0
86	10.0
87	5.0
88	3.0
89	2.0
90	3.0
91	2.5
92	2.0
93	3.0
94	4.0
95	3.5
96	3.0
97	2.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1461268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.604441379334562
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.29614300446818	9.244048100467328
2	12.492263450749661	3.648850585233542
3	6.087283355645676	2.6670411878077878
4	3.816231475141728	2.229357154747153
5	2.5686347608552205	1.875673789491556
6	1.8155230651725738	1.590882010688559
7	1.3596473482849707	1.3899822996216873
8	1.0090837231314815	1.1789683265051512
9	0.7848252690109356	1.0315741170861619
>10	4.406441685037762	11.887949710721973
>50	0.676545158989426	7.197268573322601
>100	1.6601846202104782	49.56160799770342
>500	0.016878465497762556	1.5575411769016283
>1k	0.00797038648505454	2.6435130881716784
>5k	0.0018753850553069507	1.59677584935798
>10k+	4.6884626382673767E-4	0.6989660321717951
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTT	10208	0.6985713777349535	Illumina PCR Primer Index 3 (96% over 25bp)
TTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	6506	0.4452297593596794	Illumina PCR Primer Index 3 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	6223	0.4258630175984145	Illumina PCR Primer Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	5504	0.376659175455837	TruSeq Adapter, Index 14 (96% over 26bp)
ATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	5087	0.34812231568747143	Illumina PCR Primer Index 3 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	4810	0.32916617622503197	TruSeq Adapter, Index 14 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	4749	0.324991719520307	TruSeq Adapter, Index 14 (96% over 25bp)
GGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	4240	0.2901589578366186	TruSeq Adapter, Index 14 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	3151	0.21563464059980783	Illumina PCR Primer Index 3 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	2832	0.19380428504559055	TruSeq Adapter, Index 14 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	2560	0.17519031416550557	Illumina PCR Primer Index 3 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	2489	0.17033152029607163	Illumina PCR Primer Index 3 (95% over 23bp)
CTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	2478	0.16957874941489173	Illumina PCR Primer Index 3 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	1822	0.12468623140998092	TruSeq Adapter, Index 14 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.745037871218695	0.0
2	0.0	0.0	0.0	1.5330521163811155	0.0
3	0.0	0.0	0.0	4.990049737625131	0.0
4	0.0	0.0	0.0	5.723111708461419	0.0
5	0.0	0.0	0.0	6.851583693066569	0.0
6	0.0	0.0	0.0	10.414174538825185	0.0
7	0.0	0.0	0.0	10.876444293586118	0.0
8	0.0	0.0	0.0	11.726870088170001	0.0
9	0.0	0.0	0.0	12.269960062083067	0.0
10	0.0	0.0	0.0	13.963215508722561	0.0
11	0.0	0.0	0.0	15.48969798832247	0.0
12	0.0	0.0	0.0	16.154873712419626	0.0
13	0.0	0.0	0.0	16.474664469488143	0.0
14	6.843371647090062E-5	0.0	0.0	16.637262979823003	0.0
15	6.843371647090062E-5	0.0	0.0	16.920646999729	0.0
16	6.843371647090062E-5	0.0	0.0	17.60354705639212	0.0
17	6.843371647090062E-5	0.0	0.0	18.553475474724692	0.0
18	6.843371647090062E-5	0.0	0.0	19.643350843240253	0.0
19	6.843371647090062E-5	0.0	0.0	20.258843689179535	0.0
20	6.843371647090062E-5	0.0	0.0	20.915602066150768	0.0
21	1.3686743294180124E-4	0.0	0.0	21.9542890147461	0.0
22	1.3686743294180124E-4	0.0	0.0	22.810326374080592	0.0
23	1.3686743294180124E-4	0.0	0.0	23.71132468513647	0.0
24	1.3686743294180124E-4	0.0	0.0	24.384096551761896	0.0
25	1.3686743294180124E-4	0.0	0.0	24.99069301455996	0.0
26	1.3686743294180124E-4	0.0	0.0	25.53734154172951	0.0
27	1.3686743294180124E-4	0.0	0.0	26.04327200759888	0.0
28	1.3686743294180124E-4	0.0	0.0	26.559535964655353	0.0
29	1.3686743294180124E-4	0.0	0.0	27.093592687994263	0.0
30	1.3686743294180124E-4	0.0	0.0	27.703200234317045	0.0
31	1.3686743294180124E-4	0.0	0.0	28.268462732366686	0.0
32	1.3686743294180124E-4	0.0	0.0	28.787327170649053	0.0
33	1.3686743294180124E-4	0.0	0.0	29.281076434986602	0.0
34	1.3686743294180124E-4	0.0	0.0	29.778931722312404	0.0
35	2.0530114941270185E-4	0.0	0.0	30.38511758281164	0.0
36	2.0530114941270185E-4	0.0	0.0	30.913562741399936	0.0
37	2.0530114941270185E-4	0.0	0.0	31.431674408801122	0.0
38	2.0530114941270185E-4	0.0	0.0	31.94068439191168	0.0
39	2.0530114941270185E-4	0.0	0.0	32.43368088536805	0.0
40	2.0530114941270185E-4	0.0	0.0	32.9441279765245	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	35	1.0198164E-7	46.000004	14
ACGCGAG	20	6.312019E-4	46.000004	43
CGGAATA	35	1.0198164E-7	46.000004	32
CGGGTCA	40	5.6134013E-9	46.000004	6
AGCCGTA	20	6.312019E-4	46.000004	12
AGCGTAA	55	1.8189894E-12	46.000004	45
TAATACG	40	5.6134013E-9	46.000004	38
CGGAACG	20	6.312019E-4	46.000004	37
AACCGCA	40	5.6134013E-9	46.000004	17
CCTTAAG	20	6.312019E-4	46.000004	41
ACACGTC	20	6.312019E-4	46.000004	22
CGAACAA	20	6.312019E-4	46.000004	35
GGTGTCG	35	1.0198164E-7	46.000004	15
CTCCGTC	40	5.6134013E-9	46.000004	33
CTACTCG	20	6.312019E-4	46.000004	30
CACGGAC	35	1.0198164E-7	46.000004	11
CGAAACC	20	6.312019E-4	46.000004	22
TCCGATA	35	1.0198164E-7	46.000004	36
GCGGTCC	40	5.6134013E-9	46.000004	29
CGTGCGG	105	0.0	46.000004	2
>>END_MODULE