##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2384204
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.894682669771548	31.0	31.0	34.0	31.0	34.0
2	32.553580985519694	34.0	31.0	34.0	31.0	34.0
3	32.516658809397185	34.0	31.0	34.0	31.0	34.0
4	35.98922365703606	37.0	35.0	37.0	35.0	37.0
5	35.76819391293698	37.0	35.0	37.0	35.0	37.0
6	35.767422166895116	37.0	35.0	37.0	35.0	37.0
7	35.75115342479083	37.0	35.0	37.0	33.0	37.0
8	35.70877198427652	37.0	35.0	37.0	33.0	37.0
9	37.88361985803228	39.0	38.0	39.0	35.0	39.0
10	37.80047638540997	39.0	38.0	39.0	35.0	39.0
11	37.63236031816069	39.0	37.0	39.0	35.0	39.0
12	37.165715265975564	39.0	37.0	39.0	33.0	39.0
13	36.956434516509496	39.0	37.0	39.0	33.0	39.0
14	37.81098890866721	40.0	36.0	41.0	33.0	41.0
15	37.767523248849514	40.0	36.0	41.0	33.0	41.0
16	37.78940937939874	40.0	36.0	41.0	33.0	41.0
17	37.83290314083862	40.0	36.0	41.0	33.0	41.0
18	37.883097671172436	40.0	36.0	41.0	33.0	41.0
19	37.83790145474129	40.0	36.0	41.0	33.0	41.0
20	37.7928495212658	40.0	36.0	41.0	33.0	41.0
21	37.75817044179105	40.0	36.0	41.0	33.0	41.0
22	37.67730781426422	40.0	35.0	41.0	33.0	41.0
23	37.62018308835989	40.0	35.0	41.0	32.0	41.0
24	37.599285128286006	40.0	35.0	41.0	32.0	41.0
25	37.53273209842782	40.0	35.0	41.0	32.0	41.0
26	37.43106084881998	40.0	35.0	41.0	32.0	41.0
27	37.313444235476496	40.0	35.0	41.0	32.0	41.0
28	37.22816629785035	39.0	35.0	41.0	32.0	41.0
29	37.16428250267175	39.0	35.0	41.0	31.0	41.0
30	37.0745150163325	39.0	35.0	41.0	31.0	41.0
31	37.045436128787635	39.0	35.0	41.0	31.0	41.0
32	36.95088423641601	39.0	35.0	41.0	31.0	41.0
33	36.84622708459511	39.0	35.0	41.0	31.0	41.0
34	36.80490553660677	39.0	35.0	41.0	31.0	41.0
35	36.70129108079678	39.0	35.0	41.0	30.0	41.0
36	36.626969000974746	39.0	35.0	41.0	30.0	41.0
37	36.49388768746299	39.0	35.0	41.0	30.0	41.0
38	36.380425500502476	39.0	35.0	41.0	30.0	41.0
39	36.34750507926335	39.0	35.0	41.0	30.0	41.0
40	36.29835743921242	38.0	35.0	41.0	30.0	41.0
41	36.24549954617977	38.0	35.0	41.0	30.0	41.0
42	36.09997298888853	38.0	35.0	40.0	30.0	41.0
43	35.971773807945965	38.0	35.0	40.0	30.0	41.0
44	35.91545060741446	38.0	35.0	40.0	29.0	41.0
45	35.88369535492768	38.0	35.0	40.0	29.0	41.0
46	35.80239023170836	38.0	35.0	40.0	29.0	41.0
47	35.71104402140085	38.0	35.0	40.0	29.0	41.0
48	35.61857919875984	38.0	34.0	40.0	29.0	41.0
49	35.60061303479065	37.0	34.0	40.0	29.0	41.0
50	35.493438900362555	37.0	34.0	40.0	28.0	41.0
51	35.325779589330445	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	1.0
9	14.0
10	18.0
11	33.0
12	22.0
13	27.0
14	38.0
15	85.0
16	145.0
17	307.0
18	670.0
19	1331.0
20	2226.0
21	3475.0
22	5424.0
23	8053.0
24	11335.0
25	16340.0
26	22125.0
27	27027.0
28	31302.0
29	37842.0
30	45949.0
31	56459.0
32	71349.0
33	90841.0
34	139769.0
35	253867.0
36	175871.0
37	244110.0
38	365415.0
39	772305.0
40	426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.154220024796536	90.75842503409943	3.393417677346402	3.6939372637576318
2	3.5314931104888676	2.8786966215978165	90.49858988576482	3.0912203821485074
3	27.64390127690416	16.79935945078525	42.27847113753689	13.278268134773702
4	26.777616344910083	15.991207128249094	43.45089598037752	13.780280546463306
5	24.47521269153143	14.716358163982612	45.91872172012126	14.889707424364692
6	23.274853997392842	14.552068531048517	47.45239920745037	14.720678264108273
7	23.57029851472441	17.370451521765755	44.14823563755451	14.91101432595533
8	25.146380091636455	20.6652199224563	41.83702401304586	12.351375972861382
9	81.31367953413383	4.500621591105459	9.712633650476217	4.473065224284499
10	81.42767984618766	4.955112901412798	9.604421433736375	4.0127858186631675
11	74.39304690370456	7.246401734079802	11.053416570058602	7.307134792157047
12	38.741735187089695	25.51321111784059	17.801329080900796	17.94372461416892
13	31.20156664446499	24.853410194765214	25.630189362990752	18.31483379777905
14	27.629976293974845	24.103180768088635	28.45264079751565	19.81420214042087
15	21.847585189857917	27.13731710877089	31.56743298811679	19.447664713254404
16	18.30879404614706	32.90381192213418	28.93540150087828	19.85199253084048
17	16.772935537395288	25.176159422599742	36.95308790690729	21.09781713309767
18	20.307909893616486	22.474083593518003	35.14023967747726	22.077766835388246
19	19.982434389003625	22.957137895918304	29.958006949069794	27.10242076600828
20	21.76399334956237	23.284794421953826	32.37214600764028	22.57906622084352
21	25.166386768917427	25.06115248527391	27.666885887281456	22.10557485852721
22	26.690375488003543	22.912762498511032	27.52885239685866	22.868009616626765
23	23.759460180420803	25.698304339729315	27.721998620923376	22.820236858926503
24	27.902645914527447	22.077305465471913	25.814611501364816	24.20543711863582
25	25.82664906190913	25.111567634313168	24.92005717631545	24.14172612746225
26	25.223428867664012	21.342091532435983	31.115919610905777	22.31855998899423
27	26.04982627325514	24.551632326763986	26.944170884706175	22.4543705152747
28	23.30513664099213	27.16189554249552	25.98867378798123	23.544294028531116
29	22.43121813401873	26.43809002920891	27.595541321128565	23.535150515643796
30	21.149196964689263	26.83126108336367	29.518195590645767	22.501346361301298
31	21.475301610097123	23.886085251094286	30.062654034637976	24.575959104170618
32	22.547231696616564	26.805004940852378	28.959057194770242	21.688706167760813
33	22.86360563106177	29.146499208960307	25.813562933373152	22.17633222660477
34	24.073904749761347	25.422908442398384	28.008299625367627	22.49488718247264
35	24.64772309752018	27.143860173038885	25.53959308851088	22.668823640930054
36	22.580827815069515	25.62683394541742	26.359363544394693	25.43297469511837
37	23.438556432251602	24.60066336605425	29.899538797854547	22.061241403839603
38	24.92613887066711	28.083754578047852	25.33315102231185	21.656955528973192
39	23.057800423118156	27.46275067066409	28.068109943612207	21.41133896260555
40	21.815457066593293	26.325264113305742	29.448528733279534	22.41075008682143
41	21.19558561264053	28.20366881357468	26.52923155904444	24.07151401474035
42	24.661228653252827	25.548191346042536	28.30521213788753	21.48536786281711
43	21.837351166259264	25.208455316743027	28.276816916673237	24.677376600324468
44	21.570763240058316	26.742887773026137	25.432555267921703	26.253793718993844
45	21.716388362740773	24.810251136228274	27.365988816393227	26.107371684637727
46	23.004281512823567	25.704679633118644	27.21956678203711	24.071472072020683
47	20.232790482693595	27.338516335011604	27.612695893472207	24.8159972888226
48	20.915953500623267	27.475669028321402	26.823082253028684	24.785295218026647
49	21.604149644912933	25.6400459021124	30.05195864112299	22.703845811851668
50	21.845865538351582	23.701117857364554	31.100149148311136	23.352867455972728
51	20.40928544705067	24.877317544975178	28.634630258149052	26.0787667498251
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	974.0
1	1980.0
2	2986.0
3	7171.0
4	11356.0
5	7809.0
6	4262.0
7	4287.0
8	4312.0
9	4661.5
10	5011.0
11	5198.0
12	5385.0
13	5495.0
14	5605.0
15	5391.0
16	5177.0
17	6225.5
18	7274.0
19	7187.0
20	7100.0
21	8410.0
22	9720.0
23	12526.0
24	15332.0
25	18239.5
26	25695.0
27	30243.0
28	34012.0
29	37781.0
30	43360.0
31	48939.0
32	55725.5
33	62512.0
34	74733.0
35	86954.0
36	92892.0
37	98830.0
38	113233.5
39	127637.0
40	146784.0
41	165931.0
42	189877.0
43	213823.0
44	223853.5
45	233884.0
46	222603.0
47	211322.0
48	181595.5
49	151869.0
50	139722.0
51	127575.0
52	120591.5
53	113608.0
54	109515.5
55	105423.0
56	107387.5
57	109352.0
58	106290.0
59	103228.0
60	90907.0
61	78586.0
62	67030.5
63	55475.0
64	45466.0
65	35457.0
66	33111.5
67	30766.0
68	24396.0
69	18026.0
70	15273.5
71	12521.0
72	11058.5
73	9596.0
74	7276.0
75	3456.0
76	1956.0
77	1772.0
78	1588.0
79	1052.5
80	517.0
81	311.0
82	105.0
83	71.0
84	37.0
85	27.5
86	18.0
87	23.5
88	29.0
89	23.0
90	17.0
91	9.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2384204.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.262012212330445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.47405367252051	10.908378800147696
2	11.137549130324528	3.399628216848866
3	4.805902328847801	2.200432201024274
4	2.7289375189939324	1.665963109662886
5	1.6964372992867085	1.294552338958331
6	1.15172785629228	1.0546610764808362
7	0.7777040496101769	0.8308530091910553
8	0.5712061778327893	0.6974204529474101
9	0.4187669449758695	0.5752103605507829
>10	2.6686201044359836	8.462625980721947
>50	0.7639242373057525	8.579605418029058
>100	1.784770155709941	55.675309901441025
>500	0.012681406726060312	1.2898010235676525
>1k	0.006892068872858865	2.430152585849632
>5k	5.513655098287093E-4	0.5068743412467627
>10k+	2.7568275491435463E-4	0.4285311833318069
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	10185	0.4271865998043791	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6754	0.28328112862825494	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5293	0.222002815195344	No Hit
ATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	4846	0.2032544195043713	No Hit
ATGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	4301	0.18039563728607114	No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	4027	0.1689033320974212	Illumina Single End Adapter 2 (95% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	3555	0.1491063684147833	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	3455	0.14491209644812272	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	3437	0.14415712749412382	Illumina Single End Adapter 2 (95% over 21bp)
ATGTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	3262	0.13681715155246785	No Hit
ATATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	3092	0.12968688920914484	No Hit
ATGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	2914	0.12222108510848903	No Hit
ATAGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	2483	0.10414377293218198	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.388543933321143E-5	0.0	0.0	0.17884375665840674	0.0
2	8.388543933321143E-5	0.0	0.0	0.8028255971384999	0.0
3	8.388543933321143E-5	0.0	0.0	1.1337117125883522	0.0
4	8.388543933321143E-5	0.0	0.0	1.4929930492524968	0.0
5	8.388543933321143E-5	0.0	0.0	2.8660299202585016	0.0
6	8.388543933321143E-5	0.0	0.0	3.1913376539926954	0.0
7	8.388543933321143E-5	0.0	0.0	3.71348257112227	0.0
8	8.388543933321143E-5	0.0	0.0	5.268508902761677	0.0
9	8.388543933321143E-5	0.0	0.0	5.4564961723074035	0.0
10	1.2582815899981714E-4	0.0	0.0	5.821775317883873	0.0
11	1.2582815899981714E-4	0.0	0.0	6.100526632788134	0.0
12	1.6777087866642285E-4	0.0	0.0	6.879193223398669	0.0
13	1.6777087866642285E-4	0.0	0.0	7.568479878399667	0.0
14	2.0971359833302854E-4	0.0	0.0	7.865727932676902	0.0
15	2.0971359833302854E-4	0.0	0.0	8.026410491719668	0.0
16	2.516563179996343E-4	0.0	0.0	8.13118340544685	0.0
17	2.516563179996343E-4	0.0	0.0	8.287713635242621	0.0
18	2.9359903766624E-4	0.0	0.0	8.638900027011111	0.0
19	3.355417573328457E-4	0.0	0.0	9.071245581334484	0.0
20	3.355417573328457E-4	0.0	0.0	9.546917965073458	0.0
21	3.7748447699945136E-4	0.0	0.0	9.84198499792803	0.0
22	3.7748447699945136E-4	0.0	0.0	10.167670216139223	0.0
23	4.194271966660571E-4	0.0	0.0	10.675932093059151	0.0
24	4.194271966660571E-4	0.0	0.0	11.120189379767838	0.0
25	4.613699163326628E-4	0.0	0.0	11.578203878527173	0.0
26	5.452553556658743E-4	0.0	0.0	11.963447758664946	0.0
27	5.452553556658743E-4	0.0	0.0	12.276969588172824	0.0
28	5.452553556658743E-4	0.0	0.0	12.580970420316381	0.0
29	6.710835146656914E-4	0.0	0.0	12.861651100325307	0.0
30	6.710835146656914E-4	0.0	0.0	13.150468667949555	0.0
31	7.969116736655084E-4	0.0	0.0	13.462186960511769	0.0
32	7.969116736655084E-4	0.0	0.0	13.788836861275293	0.0
33	8.388543933321142E-4	0.0	0.0	14.097199736264178	0.0
34	8.388543933321142E-4	0.0	0.0	14.397887093554075	0.0
35	9.227398326653256E-4	0.0	0.0	14.728353781807261	0.0
36	9.227398326653256E-4	0.0	0.0	15.03965264717281	0.0
37	9.227398326653256E-4	0.0	0.0	15.431313763419572	0.0
38	0.0010066252719985371	0.0	0.0	15.766603864434419	0.0
39	0.0010066252719985371	0.0	0.0	16.098790204193936	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	60	0.0	45.000004	44
GGCGTAA	30	2.1664873E-6	45.000004	24
AGGCGTA	30	2.1664873E-6	45.000004	14
CCCGTAG	30	2.1664873E-6	45.000004	24
ACGTAAC	30	2.1664873E-6	45.000004	41
TACCCGC	20	7.034813E-4	45.0	28
CGAGTTA	50	2.1827873E-11	45.0	35
GTCGACA	40	6.8212103E-9	45.0	39
ACGTTAC	50	2.1827873E-11	45.0	25
CCTAGCG	25	3.8920713E-5	45.0	23
TAGAACG	85	0.0	45.0	2
CACGCTA	25	3.8920713E-5	45.0	28
CGATTGA	20	7.034813E-4	45.0	19
CGCGACA	35	1.2126657E-7	45.0	34
ATCGTAC	50	2.1827873E-11	45.0	1
TACGATG	40	6.8212103E-9	45.0	19
CGCTAGC	25	3.8920713E-5	45.0	28
TATTCGA	35	1.2126657E-7	45.0	38
CGAATAC	25	3.8920713E-5	45.0	18
GTATCGC	70	0.0	45.0	5
>>END_MODULE