##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549077_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1643402
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.907570393610328	31.0	31.0	34.0	31.0	34.0
2	32.5566647722225	34.0	31.0	34.0	31.0	34.0
3	32.533323556865575	34.0	31.0	34.0	31.0	34.0
4	36.01204452714552	37.0	35.0	37.0	35.0	37.0
5	35.791192295007555	37.0	35.0	37.0	35.0	37.0
6	35.802813918931584	37.0	35.0	37.0	35.0	37.0
7	35.7940601264937	37.0	35.0	37.0	35.0	37.0
8	35.766239179458225	37.0	35.0	37.0	33.0	37.0
9	37.91203308746125	39.0	38.0	39.0	35.0	39.0
10	37.82827816930976	39.0	38.0	39.0	35.0	39.0
11	37.658614873293324	39.0	38.0	39.0	35.0	39.0
12	37.21017864162268	39.0	37.0	39.0	33.0	39.0
13	36.99031034403025	39.0	37.0	39.0	33.0	39.0
14	37.70401094802124	40.0	36.0	41.0	33.0	41.0
15	37.5857787686762	40.0	36.0	41.0	33.0	41.0
16	37.64318833736359	40.0	36.0	41.0	33.0	41.0
17	37.734520220858926	40.0	35.0	41.0	33.0	41.0
18	37.78030451465923	40.0	35.0	41.0	33.0	41.0
19	37.74605300468175	40.0	35.0	41.0	33.0	41.0
20	37.70673517496024	40.0	36.0	41.0	33.0	41.0
21	37.68012452218021	40.0	36.0	41.0	33.0	41.0
22	37.57523113638659	40.0	35.0	41.0	33.0	41.0
23	37.51034622082729	39.0	35.0	41.0	32.0	41.0
24	37.46900575756875	39.0	35.0	41.0	32.0	41.0
25	37.41679455178952	39.0	35.0	41.0	32.0	41.0
26	37.3056452407871	39.0	35.0	41.0	32.0	41.0
27	37.21663232733074	39.0	35.0	41.0	32.0	41.0
28	37.114374936868764	39.0	35.0	41.0	32.0	41.0
29	37.016377003313856	39.0	35.0	41.0	31.0	41.0
30	36.96781371812861	39.0	35.0	41.0	31.0	41.0
31	36.89483340047049	39.0	35.0	41.0	31.0	41.0
32	36.7968038252357	39.0	35.0	41.0	31.0	41.0
33	36.689018876696025	39.0	35.0	41.0	31.0	41.0
34	36.607525121668345	39.0	35.0	41.0	31.0	41.0
35	36.5225100127662	39.0	35.0	41.0	30.0	41.0
36	36.42178663528461	38.0	35.0	41.0	30.0	41.0
37	36.30143628886906	38.0	35.0	41.0	30.0	41.0
38	36.19955981555334	38.0	35.0	41.0	30.0	41.0
39	36.13980693707322	38.0	35.0	41.0	30.0	41.0
40	36.07655217652163	38.0	35.0	41.0	30.0	41.0
41	36.00082937710919	38.0	35.0	40.0	30.0	41.0
42	35.814742832246765	38.0	35.0	40.0	29.0	41.0
43	35.694362061139024	38.0	35.0	40.0	29.0	41.0
44	35.62795165151314	37.0	35.0	40.0	29.0	41.0
45	35.604766819074094	37.0	35.0	40.0	29.0	41.0
46	35.523558447659184	37.0	34.0	40.0	29.0	41.0
47	35.41460214847007	37.0	34.0	40.0	28.0	41.0
48	35.34634009207729	37.0	34.0	40.0	28.0	41.0
49	35.305962874573595	37.0	34.0	40.0	28.0	41.0
50	35.20766069409676	36.0	34.0	40.0	28.0	41.0
51	35.05954842454859	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	15.0
11	22.0
12	20.0
13	37.0
14	34.0
15	56.0
16	107.0
17	220.0
18	393.0
19	899.0
20	1510.0
21	2543.0
22	3818.0
23	5630.0
24	7927.0
25	11629.0
26	15359.0
27	19103.0
28	22227.0
29	26494.0
30	32037.0
31	39850.0
32	49605.0
33	64698.0
34	105188.0
35	198496.0
36	124738.0
37	167972.0
38	249666.0
39	492868.0
40	233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.372456647856094	90.24444414695857	3.7299455641407278	3.653153641044614
2	3.620842617935234	2.9132859762857777	90.41567431462296	3.0501970911560288
3	28.550774551813856	16.06934882639792	42.37538958818354	13.004487033604681
4	27.45439034393289	15.136588613133	43.35390853850732	14.055112504426791
5	25.134264166649427	14.516837633153665	45.50000547644459	14.848892723752313
6	23.20570377789488	15.010995483758691	45.98941707506745	15.79388366327898
7	22.592889627735634	17.578900354265116	43.97146894064873	15.85674107735052
8	25.978914471322295	19.64954405556279	43.05939751807531	11.312143955039607
9	82.0634878136938	4.436346067486835	9.004187654633498	4.495978464185877
10	82.57200611901409	4.58262798755265	9.000475842185905	3.8448900512473516
11	75.88526726875105	7.0373530030996685	10.288839857807158	6.788539870342132
12	42.83626282552899	23.0477387760268	16.357105565162996	17.758892833281205
13	34.49442071994558	23.584308647549413	23.62836360184544	18.29290703065957
14	29.18458173958654	23.95080448971098	27.833968803737612	19.030644966964868
15	22.01774124651181	27.60566191351842	30.58770769416126	19.78888914580851
16	18.417161473577373	32.44750827855874	29.956030234842114	19.17930001302177
17	17.55030114360333	23.175339935085876	39.30243482726685	19.971924094043942
18	21.403831807433605	20.336716153442673	36.320389046624015	21.939062992499707
19	21.41046439033176	21.10761700423877	29.68865803984661	27.793260565582855
20	22.572565933350454	22.64418565877369	31.483288933565863	23.299959474309997
21	26.370906205541917	23.399448217782382	28.207218927566107	22.02242664910959
22	27.779934550402153	22.715440287890605	27.312732977080472	22.19189218462677
23	25.98956311358998	23.861842689737507	28.180810294742248	21.967783901930265
24	28.291251927404247	21.282254737428822	24.924759736205747	25.50173359896118
25	26.550290190714144	24.533315646445605	24.736917686603764	24.179476476236488
26	26.39682804329069	21.288400525251884	31.279321797101378	21.03544963435605
27	26.709593879038724	22.65641638503543	27.90972628729915	22.72426344862669
28	24.862145719671755	26.325147468483063	25.123128729306643	23.68957808253854
29	23.05747467752869	27.2849856578001	26.506782880877594	23.150756783793618
30	22.955308561143287	25.18367386677149	29.609614689528186	22.251402882557038
31	22.321927319061313	24.702355236272076	28.14886436793919	24.826853076727424
32	24.015426535929734	26.907050131373822	27.1298805769982	21.94764275569824
33	26.8496083125127	26.19651186988941	24.22006301562247	22.733816801975415
34	27.11874513965542	25.187933323678564	25.377053210352674	22.31626832631334
35	27.620326615155633	24.90717426411797	24.721035997278815	22.75146312344758
36	22.655260246732084	26.28644726001307	26.069214957752273	24.989077535502574
37	24.874984939777363	25.110046111663486	27.182271896955218	22.83269705160393
38	28.010371169074883	26.38964781593305	23.593740302129362	22.006240712862706
39	24.80366946127606	28.416236562934692	25.263812506008875	21.51628146978037
40	24.415815485194734	26.93650123341702	26.082236726011043	22.5654465553772
41	24.12848469212037	27.84388725339266	25.003742237139786	23.023885817347185
42	25.58783547786847	24.839448899295487	26.698762688617876	22.873952934218163
43	22.47587626155986	24.82478419765827	27.6550107642561	25.04432877652577
44	23.1807555302963	25.834275484634922	25.293385306820852	25.691583678247927
45	23.399569916551155	23.662804353408358	27.056800466349685	25.88082526369081
46	23.711788107839713	24.69146319646684	27.352650173238196	24.24409852245525
47	21.69816027971245	27.342184079123673	26.528627809872447	24.43102783129143
48	22.729739893221502	27.01676157142318	25.64868486225525	24.60481367310007
49	23.769229926700834	24.910642679028015	28.26971124533133	23.05041614893982
50	23.680694072418067	22.782557158869224	30.35495879888183	23.181789969830877
51	22.59240283266054	24.734483711228293	27.792165276663894	24.88094817944727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	739.0
1	1550.5
2	2362.0
3	4980.0
4	7598.0
5	5487.5
6	3377.0
7	3401.5
8	3426.0
9	3425.0
10	3424.0
11	3708.0
12	3992.0
13	4101.0
14	4210.0
15	4021.5
16	3833.0
17	3729.5
18	3626.0
19	4551.0
20	5476.0
21	6290.0
22	7104.0
23	7610.5
24	8117.0
25	9737.5
26	14232.5
27	17107.0
28	19438.5
29	21770.0
30	23805.0
31	25840.0
32	32090.0
33	38340.0
34	42242.0
35	46144.0
36	49260.5
37	52377.0
38	65123.5
39	77870.0
40	89064.5
41	100259.0
42	107723.0
43	115187.0
44	119235.5
45	123284.0
46	128461.5
47	133639.0
48	139897.0
49	146155.0
50	135610.0
51	125065.0
52	112335.5
53	99606.0
54	96546.0
55	93486.0
56	91024.5
57	88563.0
58	80171.5
59	71780.0
60	67349.5
61	62919.0
62	54768.5
63	46618.0
64	38503.0
65	30388.0
66	25560.5
67	20733.0
68	17289.5
69	13846.0
70	11883.5
71	9921.0
72	8530.0
73	7139.0
74	5394.5
75	2695.0
76	1740.0
77	1264.5
78	789.0
79	582.0
80	375.0
81	219.5
82	64.0
83	59.0
84	54.0
85	31.5
86	9.0
87	6.5
88	4.0
89	5.5
90	7.0
91	9.0
92	11.0
93	15.0
94	19.0
95	10.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1643402.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.73560542329398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3570421618406	11.54317470478664
2	10.997559130510444	3.461065021941127
3	4.38977505055858	2.0722730427783063
4	2.249315706403214	1.4157737771351495
5	1.3161453456964518	1.035517191979211
6	0.8628493210069242	0.8146473873073252
7	0.5821002885574021	0.6411790320267394
8	0.423297519661241	0.5328674196838652
9	0.31900880042781726	0.4517816949081422
>10	2.5110975610871287	9.083306614679595
>50	1.1103901371963705	12.894982525768683
>100	1.8628025864336122	52.734236291657766
>500	0.011247402666369926	1.2103921946903748
>1k	0.007368987953828573	2.108803100657047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	4928	0.2998657662580428	Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4078	0.24814378952928134	No Hit
ATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG	2823	0.17177781212387475	Illumina PCR Primer Index 8 (95% over 22bp)
ATGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT	1939	0.11798695632596284	Illumina PCR Primer Index 8 (95% over 21bp)
ATGTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC	1866	0.11354495126572806	No Hit
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	1859	0.1131190055750206	Illumina PCR Primer Index 8 (95% over 24bp)
ATGCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC	1830	0.11135437342780403	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12401104538025388	0.0
2	0.0	0.0	0.0	0.5729578033859032	0.0
3	0.0	0.0	0.0	0.8163553409330158	0.0
4	0.0	0.0	0.0	1.0723487010481916	0.0
5	0.0	0.0	0.0	2.063402624555647	0.0
6	0.0	0.0	0.0	2.2884844974023397	0.0
7	0.0	0.0	0.0	2.667576162131968	0.0
8	0.0	0.0	0.0	3.815682346741698	0.0
9	1.2169876877355632E-4	0.0	0.0	3.945656631791856	0.0
10	1.2169876877355632E-4	0.0	0.0	4.212724579865426	0.0
11	2.4339753754711263E-4	0.0	0.0	4.420099281855565	0.0
12	2.4339753754711263E-4	0.0	0.0	4.995551910001327	0.0
13	2.4339753754711263E-4	0.0	0.0	5.509850906838375	0.0
14	2.4339753754711263E-4	0.0	0.0	5.732863900615918	0.0
15	3.042469219338908E-4	0.0	0.0	5.859126373218482	0.0
16	3.042469219338908E-4	0.0	0.0	5.955633496855913	0.0
17	3.042469219338908E-4	0.0	0.0	6.084999288062202	0.0
18	3.042469219338908E-4	0.0	0.0	6.363932866091194	0.0
19	3.042469219338908E-4	0.0	0.0	6.719354120294365	0.0
20	3.042469219338908E-4	0.0	0.0	7.116883148493186	0.0
21	3.6509630632066895E-4	0.0	0.0	7.35723821682096	0.0
22	3.6509630632066895E-4	0.0	0.0	7.598506025914536	0.0
23	3.6509630632066895E-4	0.0	0.0	7.977354293106616	0.0
24	4.2594569070744713E-4	0.0	0.0	8.3285769397871	0.0
25	4.2594569070744713E-4	0.0	0.0	8.713266747880311	0.0
26	4.8679507509422526E-4	0.0	0.0	9.0051003953993	0.0
27	4.8679507509422526E-4	0.0	0.0	9.270160313788105	0.0
28	5.476444594810034E-4	0.0	0.0	9.524206493602904	0.0
29	5.476444594810034E-4	0.0	0.0	9.759024267951482	0.0
30	5.476444594810034E-4	0.0	0.0	9.99323354845619	0.0
31	5.476444594810034E-4	0.0	0.0	10.245149999817452	0.0
32	5.476444594810034E-4	0.0	0.0	10.520615162936396	0.0
33	5.476444594810034E-4	0.0	0.0	10.79346380252671	0.0
34	5.476444594810034E-4	0.0	0.0	11.079151662222634	0.0
35	5.476444594810034E-4	0.0	0.0	11.357659294560916	0.0
36	6.084938438677816E-4	0.0	0.0	11.632698511989155	0.0
37	6.084938438677816E-4	0.0	0.0	11.942543577286628	0.0
38	6.084938438677816E-4	0.0	0.0	12.232673442042787	0.0
39	6.084938438677816E-4	0.0	0.0	12.513067405297061	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	20	7.034174E-4	45.0	30
CGAACTA	20	7.034174E-4	45.0	21
CGAACGA	40	6.8175723E-9	45.0	3
ACCGGCA	50	2.1827873E-11	45.0	33
CGAGTTA	25	3.89154E-5	45.0	42
ACGTTCC	25	3.89154E-5	45.0	37
CGTTGTC	40	6.8175723E-9	45.0	21
CGCATCG	35	1.2123564E-7	45.0	18
CGCATCC	25	3.89154E-5	45.0	17
ATTACGA	50	2.1827873E-11	45.0	1
GTGATCG	25	3.89154E-5	45.0	12
ATCGTAC	100	0.0	45.0	1
GTTCGAC	25	3.89154E-5	45.0	25
CGGATGT	20	7.034174E-4	45.0	35
CGCTAAC	20	7.034174E-4	45.0	16
TCTAACG	20	7.034174E-4	45.0	2
GTCACGA	20	7.034174E-4	45.0	27
GTCGTAG	40	6.8175723E-9	45.0	3
AACGATC	20	7.034174E-4	45.0	28
GGACGTA	55	1.8189894E-12	45.0	11
>>END_MODULE