FastQCFastQC Report
Sat 18 Jun 2016
SRR3549076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334144
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT52620.39441019859925164Illumina PCR Primer Index 8 (96% over 25bp)
GTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC36430.27305898014007485Illumina PCR Primer Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT29740.22291446800345388Illumina PCR Primer Index 8 (95% over 24bp)
TGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC29490.22104060731075506Illumina PCR Primer Index 8 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC28860.21631847836515397Illumina PCR Primer Index 8 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC26780.20072795740189966Illumina PCR Primer Index 8 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC22720.1702964597524705Illumina PCR Primer Index 8 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC18440.1382159646934664Illumina PCR Primer Index 8 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC16760.12562362083853018Illumina PCR Primer Index 8 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC14550.10905869231507244Illumina PCR Primer Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT14090.10561078864050658Illumina PCR Primer Index 8 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC206.31182E-446.00000429
CGAACGT206.31182E-446.00000411
ATTAGCG206.31182E-446.0000041
GCGAATT206.31182E-446.00000429
GCGAATA206.31182E-446.00000424
TTTCGAT206.31182E-446.00000436
CGCATCG405.6134013E-946.00000416
CTCCCGA206.31182E-446.00000435
TAACGTA206.31182E-446.00000423
TAACGGA405.6134013E-946.00000435
CGTTATA206.31182E-446.00000414
CTAACCG206.31182E-446.00000429
CGCTAAT206.31182E-446.00000432
TCGCTAA206.31182E-446.00000431
GTCGTAG206.31182E-446.0000041
GATGCGA206.31182E-446.0000049
CGAGCAT206.31182E-446.00000414
CGTGTCA206.31182E-446.00000413
CCTATTA206.31182E-446.00000429
ATCGGAT206.31182E-446.00000414
Produced by FastQC (version 0.11.5)