##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549076_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1334144
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54332066103809	33.0	31.0	34.0	31.0	34.0
2	32.95733668929291	34.0	31.0	34.0	31.0	34.0
3	33.040422173318625	34.0	33.0	34.0	31.0	34.0
4	36.42850921639643	37.0	37.0	37.0	35.0	37.0
5	36.40424421951454	37.0	37.0	37.0	35.0	37.0
6	36.41466363451022	37.0	37.0	37.0	35.0	37.0
7	36.47227885445649	37.0	37.0	37.0	35.0	37.0
8	36.503296495730595	37.0	37.0	37.0	35.0	37.0
9	38.31211548378586	39.0	39.0	39.0	37.0	39.0
10	38.07979198647222	39.0	39.0	39.0	35.0	39.0
11	37.921116461191595	39.0	38.0	39.0	35.0	39.0
12	37.535147630240814	39.0	37.0	39.0	35.0	39.0
13	37.462558014727044	39.0	37.0	39.0	35.0	39.0
14	38.79842955483066	41.0	38.0	41.0	35.0	41.0
15	38.85697945649046	41.0	38.0	41.0	35.0	41.0
16	38.902745130960376	41.0	38.0	41.0	35.0	41.0
17	38.85543464573539	41.0	38.0	41.0	35.0	41.0
18	38.85127917226326	41.0	38.0	41.0	35.0	41.0
19	38.81174071164732	40.0	38.0	41.0	35.0	41.0
20	38.71774486112444	40.0	38.0	41.0	35.0	41.0
21	38.66148031996546	40.0	37.0	41.0	35.0	41.0
22	38.74334479636381	40.0	38.0	41.0	35.0	41.0
23	38.754703390338676	40.0	38.0	41.0	35.0	41.0
24	38.69527127506476	40.0	37.0	41.0	35.0	41.0
25	38.61630978365154	40.0	37.0	41.0	35.0	41.0
26	38.51289965700854	40.0	37.0	41.0	35.0	41.0
27	38.39644820948863	40.0	36.0	41.0	35.0	41.0
28	38.36746483138252	40.0	36.0	41.0	35.0	41.0
29	38.3358557996738	40.0	36.0	41.0	35.0	41.0
30	38.28994996042406	40.0	36.0	41.0	35.0	41.0
31	38.21289456010746	40.0	36.0	41.0	35.0	41.0
32	38.14957305957977	40.0	35.0	41.0	34.0	41.0
33	38.102806743499954	40.0	35.0	41.0	34.0	41.0
34	37.99215826777319	40.0	35.0	41.0	34.0	41.0
35	37.92612266741821	40.0	35.0	41.0	34.0	41.0
36	37.82134237383671	40.0	35.0	41.0	34.0	41.0
37	37.74601242444594	40.0	35.0	41.0	34.0	41.0
38	37.670792658063895	40.0	35.0	41.0	33.0	41.0
39	37.574036985512805	40.0	35.0	41.0	33.0	41.0
40	37.383469850331	39.0	35.0	41.0	33.0	41.0
41	37.23677129305382	39.0	35.0	41.0	33.0	41.0
42	37.18025190684064	39.0	35.0	41.0	33.0	41.0
43	37.12132348532092	39.0	35.0	41.0	33.0	41.0
44	36.981804063129616	39.0	35.0	41.0	33.0	41.0
45	36.86182226206466	39.0	35.0	41.0	33.0	41.0
46	36.837763389858964	38.0	35.0	41.0	33.0	41.0
47	36.79106603185264	38.0	35.0	41.0	33.0	41.0
48	36.697221589273724	38.0	35.0	41.0	33.0	41.0
49	36.62779729924206	38.0	35.0	40.0	32.0	41.0
50	36.52268645663437	38.0	35.0	40.0	32.0	41.0
51	36.40781429890627	37.0	35.0	40.0	32.0	41.0
52	36.07744366425214	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	10.0
12	11.0
13	28.0
14	44.0
15	64.0
16	62.0
17	106.0
18	198.0
19	384.0
20	603.0
21	968.0
22	1408.0
23	1940.0
24	2737.0
25	3639.0
26	4745.0
27	5975.0
28	7587.0
29	9979.0
30	12854.0
31	17003.0
32	22268.0
33	31655.0
34	72839.0
35	154669.0
36	84743.0
37	116437.0
38	197413.0
39	582645.0
40	1126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.32610272954044	13.538268732610574	46.40645987239758	11.729168665451406
2	26.17393624676197	12.697729780293582	49.0943256500048	12.034008322939654
3	26.443997049793726	13.916114002686367	44.687529981771085	14.952358965748825
4	24.088104432505038	14.641897726182481	45.7959560587163	15.474041782596181
5	23.039941715437013	16.94862023889475	43.84376798906265	16.167670056605584
6	25.38421639643097	19.47480931593591	43.81146263072052	11.329511656912597
7	86.87158207809652	2.945409191211743	5.9853359157632156	4.197672814928524
8	87.69090892737216	2.8076429530845246	6.335448047587066	3.1660000719562507
9	80.29388132015734	4.9059172023409765	8.240939508778663	6.559261968723017
10	44.414096229492465	22.851281420896093	13.794987647510315	18.93963470210112
11	35.189454811474626	22.66861748057181	23.358947759762064	18.7829799481915
12	29.742591504365347	24.020795356423292	26.58918377626403	19.647429362947328
13	22.774003405929193	26.46880696536506	31.14431425693179	19.61287537177396
14	18.343297275256646	33.4325979804279	28.849884270363617	19.374220473951837
15	17.673654418113788	23.210537992900313	38.070328240429816	21.04547934855608
16	21.515593519140364	21.212103041350858	34.68298774345198	22.589315696056797
17	21.593920896095174	21.3387760241773	28.135418665451407	28.93188441427612
18	22.678361556173847	23.17853245226902	29.50670992036842	24.636396071188717
19	26.02777511273146	24.34512316511561	27.222998416962486	22.404103305190446
20	28.396035210591958	22.251046363810804	25.48922754964981	23.863690875947423
21	26.338611124436344	24.3949678595414	27.401689772618248	21.86473124340401
22	28.891633886596953	20.76987191787393	24.18029837858582	26.158195816943298
23	27.644617072819727	24.25795116569126	22.594862323707186	25.50256943778183
24	27.41877938213566	20.24106843039432	29.628360956538422	22.711791230931595
25	28.15992876331191	22.917990861556174	25.328900028782503	23.593180346349417
26	24.811189796603664	26.585061282740096	23.795857118871726	24.807891801784514
27	23.402646191115803	26.95975846685215	24.537980907608173	25.099614434423874
28	24.696509522210498	23.90296699606639	28.294172143336848	23.10635133838626
29	23.79855547826921	23.427905833253384	26.534317135181812	26.239221553295593
30	25.35723280245611	27.174128130096904	25.148334812434044	22.320304255012953
31	28.01429231027535	26.97220090185167	22.032779070325244	22.98072771754773
32	28.712717667658065	25.874793125779526	22.628966588314306	22.783522618248107
33	29.481525232658544	24.670425381368126	23.208439268924494	22.639610117048832
34	22.44817650868272	26.91561030893217	24.649887868176148	25.986325314208962
35	25.409101266430014	24.925495298858294	26.10362899357191	23.56177444113979
36	29.51428031756692	24.85743667849947	22.519083397294445	23.109199606639162
37	26.384183416482777	27.395168737407655	23.672931857430683	22.54771598867888
38	24.779633982538616	25.94907296363811	26.48814520771371	22.783147846109568
39	24.998575865873548	28.16075326201669	23.480898613642907	23.35977225846685
40	27.101872061786437	23.915784203204453	26.194473759953947	22.787869975055166
41	23.11961827209057	24.16096013623717	26.76112923342608	25.958292358246187
42	23.733794852729538	25.644832941571526	23.815720042214334	26.8056521634846
43	24.6814436822412	22.495247889283316	26.41311582557805	26.410192602897435
44	26.600801712558763	23.352876451117723	25.148859493427995	24.89746234289552
45	22.5874418353641	26.069000047970835	25.581121678019763	25.762436438645302
46	23.66970881703924	26.278122901276024	24.373830710927756	25.67833757075698
47	25.298318622277655	24.0594718411206	27.51614518372829	23.126064352873453
48	24.20338434231987	21.71227393744603	30.360965532955962	23.723376187278138
49	23.292912908951358	23.269901899645014	26.54511057277176	26.892074618631874
50	22.589690468195336	24.906456874220474	27.99382975151108	24.510022906073107
51	23.44926784515015	25.41524752950206	26.113223160318526	25.02226146502926
52	23.609445457162046	23.926352777511273	26.377587426844478	26.0866143384822
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	154.0
1	119.5
2	85.0
3	100.0
4	115.0
5	126.5
6	138.0
7	226.5
8	315.0
9	450.0
10	585.0
11	815.5
12	1046.0
13	1108.0
14	1037.0
15	904.0
16	952.5
17	1001.0
18	1032.0
19	1063.0
20	1473.5
21	1884.0
22	2805.5
23	3727.0
24	4315.0
25	4903.0
26	5463.5
27	6024.0
28	8759.5
29	11495.0
30	11947.0
31	12399.0
32	16286.0
33	20173.0
34	24344.5
35	28516.0
36	28552.5
37	28589.0
38	33002.0
39	45420.5
40	53426.0
41	63603.5
42	73781.0
43	78044.5
44	82308.0
45	84464.5
46	86621.0
47	94599.0
48	102577.0
49	113473.0
50	124369.0
51	127494.5
52	130620.0
53	114776.5
54	98933.0
55	88130.5
56	77328.0
57	75548.0
58	73768.0
59	70192.0
60	66616.0
61	60645.0
62	54674.0
63	50331.5
64	38099.0
65	30209.0
66	26286.0
67	22363.0
68	19941.5
69	17520.0
70	14264.0
71	11008.0
72	9723.5
73	8439.0
74	6962.0
75	5485.0
76	4579.5
77	3674.0
78	2434.5
79	1195.0
80	1035.5
81	876.0
82	593.0
83	310.0
84	252.5
85	195.0
86	114.5
87	34.0
88	32.5
89	30.0
90	29.0
91	18.0
92	7.0
93	8.5
94	10.0
95	14.0
96	18.0
97	20.0
98	22.0
99	15.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1334144.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.33714288882291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.42102754966005	11.341412465677077
2	12.162146928214234	3.9738946440218914
3	5.130185047122515	2.514376984828296
4	2.6410874121202714	1.7259128973472155
5	1.5694489941378127	1.2820156236974416
6	1.0849906862607774	1.0635388724690609
7	0.7525097339783934	0.8605701334464588
8	0.5261878407612978	0.6877124752702813
9	0.38254124379639576	0.5624667864692782
>10	3.189438924649462	11.728102325550562
>50	1.3618955256966931	16.334028267975977
>100	1.7675274813197381	44.998377509836246
>500	0.00504745646275107	0.5617844225666673
>1k	0.005506316141182985	1.9713430691534095
>5k	4.588596784319154E-4	0.39446352169012583
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT	5262	0.39441019859925164	Illumina PCR Primer Index 8 (96% over 25bp)
GTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	3643	0.27305898014007485	Illumina PCR Primer Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	2974	0.22291446800345388	Illumina PCR Primer Index 8 (95% over 24bp)
TGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	2949	0.22104060731075506	Illumina PCR Primer Index 8 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	2886	0.21631847836515397	Illumina PCR Primer Index 8 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	2678	0.20072795740189966	Illumina PCR Primer Index 8 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	2272	0.1702964597524705	Illumina PCR Primer Index 8 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1844	0.1382159646934664	Illumina PCR Primer Index 8 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1676	0.12562362083853018	Illumina PCR Primer Index 8 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1455	0.10905869231507244	Illumina PCR Primer Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	1409	0.10561078864050658	Illumina PCR Primer Index 8 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4154723927851866	0.0
2	0.0	0.0	0.0	0.8433122661421856	0.0
3	0.0	0.0	0.0	2.738160198599252	0.0
4	0.0	0.0	0.0	3.1355685743068213	0.0
5	0.0	0.0	0.0	3.827173078768109	0.0
6	0.0	0.0	0.0	5.933992132783268	0.0
7	0.0	0.0	0.0	6.1726470306053915	0.0
8	0.0	0.0	0.0	6.666896526911637	0.0
9	0.0	0.0	0.0	6.977957401899645	0.0
10	0.0	0.0	0.0	8.050630216828168	0.0
11	0.0	0.0	0.0	8.981564208960952	0.0
12	0.0	0.0	0.0	9.393513743643865	0.0
13	0.0	0.0	0.0	9.598439148997409	0.0
14	0.0	0.0	0.0	9.728185263359878	0.0
15	0.0	0.0	0.0	9.922766957689724	0.0
16	0.0	0.0	0.0	10.385985320924878	0.0
17	0.0	0.0	0.0	11.012004701141706	0.0
18	0.0	0.0	0.0	11.684795644248297	0.0
19	7.495442770795357E-5	0.0	0.0	12.075308212606735	0.0
20	1.4990885541590714E-4	0.0	0.0	12.479237623524897	0.0
21	1.4990885541590714E-4	0.0	0.0	13.140860356903003	0.0
22	1.4990885541590714E-4	0.0	0.0	13.705267197543893	0.0
23	1.4990885541590714E-4	0.0	0.0	14.31449678595414	0.0
24	2.248632831238607E-4	0.0	0.0	14.785585364098628	0.0
25	2.248632831238607E-4	0.0	0.0	15.18067015254725	0.0
26	2.248632831238607E-4	0.0	0.0	15.550945025424541	0.0
27	2.248632831238607E-4	0.0	0.0	15.896859709296747	0.0
28	2.248632831238607E-4	0.0	0.0	16.254392329463688	0.0
29	2.248632831238607E-4	0.0	0.0	16.61837103041351	0.0
30	2.248632831238607E-4	0.0	0.0	17.004911014103424	0.0
31	2.998177108318143E-4	0.0	0.0	17.382081694329848	0.0
32	2.998177108318143E-4	0.0	0.0	17.787285330519044	0.0
33	2.998177108318143E-4	0.0	0.0	18.173825314208962	0.0
34	3.747721385397678E-4	0.0	0.0	18.544025232658544	0.0
35	3.747721385397678E-4	0.0	0.0	18.96159634941955	0.0
36	3.747721385397678E-4	0.0	0.0	19.361852993380026	0.0
37	3.747721385397678E-4	0.0	0.0	19.740597716588315	0.0
38	3.747721385397678E-4	0.0	0.0	20.1183680322364	0.0
39	4.497265662477214E-4	0.0	0.0	20.507831238606926	0.0
40	4.497265662477214E-4	0.0	0.0	20.89856867024849	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	6.31182E-4	46.000004	29
CGAACGT	20	6.31182E-4	46.000004	11
ATTAGCG	20	6.31182E-4	46.000004	1
GCGAATT	20	6.31182E-4	46.000004	29
GCGAATA	20	6.31182E-4	46.000004	24
TTTCGAT	20	6.31182E-4	46.000004	36
CGCATCG	40	5.6134013E-9	46.000004	16
CTCCCGA	20	6.31182E-4	46.000004	35
TAACGTA	20	6.31182E-4	46.000004	23
TAACGGA	40	5.6134013E-9	46.000004	35
CGTTATA	20	6.31182E-4	46.000004	14
CTAACCG	20	6.31182E-4	46.000004	29
CGCTAAT	20	6.31182E-4	46.000004	32
TCGCTAA	20	6.31182E-4	46.000004	31
GTCGTAG	20	6.31182E-4	46.000004	1
GATGCGA	20	6.31182E-4	46.000004	9
CGAGCAT	20	6.31182E-4	46.000004	14
CGTGTCA	20	6.31182E-4	46.000004	13
CCTATTA	20	6.31182E-4	46.000004	29
ATCGGAT	20	6.31182E-4	46.000004	14
>>END_MODULE