FastQCFastQC Report
Sat 18 Jun 2016
SRR3549075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374834
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC62120.4518363671541437TruSeq Adapter, Index 10 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37660.2739239791858508No Hit
ATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG29120.21180738911024893TruSeq Adapter, Index 10 (95% over 22bp)
ATGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT25360.1844586328240355TruSeq Adapter, Index 10 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT22520.16380159350147TruSeq Adapter, Index 10 (95% over 24bp)
ATGCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC20630.15005447930441057No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC20050.14583578817515425RNA PCR Primer, Index 10 (95% over 23bp)
ATTGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC19680.1431445541789045No Hit
ATGTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC17430.12677894203954804No Hit
ATATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC16120.11725051897174496No Hit
ATGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC15280.11114069043971855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG551.8189894E-1245.00000440
TAACGCG551.8189894E-1245.0000043
TCGATGT750.045.0000042
AGCTAGT551.8189894E-1245.00000416
ATCGCAC850.045.0000041
CGCTTGT551.8189894E-1245.00000428
CTTAACG253.8912065E-545.04
AACCGTA453.8562575E-1045.026
AGCCGTC207.03377E-445.037
AACCGGT253.8912065E-545.033
CGTATGA406.8175723E-945.011
CTACTCG253.8912065E-545.026
GTCGAAG207.03377E-445.035
GTCGAAA207.03377E-445.012
GGTCGAA207.03377E-445.011
TCCGATT253.8912065E-545.030
CTATCGA253.8912065E-545.038
TCCGACA406.8175723E-945.022
TAGGTCG351.2121563E-745.02
GGCGTAA253.8912065E-545.09
Produced by FastQC (version 0.11.5)