##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549075_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1374834
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.923920997007638	31.0	31.0	34.0	31.0	34.0
2	32.597197188896985	34.0	31.0	34.0	31.0	34.0
3	32.55588529233347	34.0	31.0	34.0	31.0	34.0
4	36.03343603664151	37.0	35.0	37.0	35.0	37.0
5	35.80292820806003	37.0	35.0	37.0	35.0	37.0
6	35.81482637176561	37.0	35.0	37.0	35.0	37.0
7	35.802706363095474	37.0	35.0	37.0	35.0	37.0
8	35.767430104288955	37.0	35.0	37.0	33.0	37.0
9	37.93096039230918	39.0	38.0	39.0	35.0	39.0
10	37.86025949314608	39.0	38.0	39.0	35.0	39.0
11	37.68723787744557	39.0	38.0	39.0	35.0	39.0
12	37.23737629415624	39.0	37.0	39.0	34.0	39.0
13	36.975805806373714	39.0	37.0	39.0	33.0	39.0
14	37.648777234197	40.0	35.0	41.0	33.0	41.0
15	37.49966104998858	40.0	35.0	41.0	32.0	41.0
16	37.53740960726895	40.0	35.0	41.0	33.0	41.0
17	37.624620863318775	40.0	35.0	41.0	33.0	41.0
18	37.68673308923113	40.0	35.0	41.0	33.0	41.0
19	37.63147841848543	40.0	35.0	41.0	33.0	41.0
20	37.613571529362815	39.0	35.0	41.0	33.0	41.0
21	37.589510442715266	39.0	35.0	41.0	33.0	41.0
22	37.52480517647949	39.0	35.0	41.0	33.0	41.0
23	37.439319219629425	39.0	35.0	41.0	32.0	41.0
24	37.37098224221979	39.0	35.0	41.0	32.0	41.0
25	37.314186294490824	39.0	35.0	41.0	32.0	41.0
26	37.2212281628182	39.0	35.0	41.0	32.0	41.0
27	37.130255725418486	39.0	35.0	41.0	32.0	41.0
28	37.02881802457606	39.0	35.0	41.0	32.0	41.0
29	36.94970083661009	39.0	35.0	41.0	31.0	41.0
30	36.88850944914077	39.0	35.0	41.0	31.0	41.0
31	36.81328364006127	39.0	35.0	41.0	31.0	41.0
32	36.71522598364603	39.0	35.0	41.0	31.0	41.0
33	36.59919015677529	39.0	35.0	41.0	31.0	41.0
34	36.48791054047252	38.0	35.0	41.0	31.0	41.0
35	36.433767276631215	38.0	35.0	41.0	30.0	41.0
36	36.28402774444042	38.0	35.0	41.0	30.0	41.0
37	36.17715084148341	38.0	35.0	41.0	30.0	41.0
38	36.087794599202525	38.0	35.0	41.0	30.0	41.0
39	35.993519944953356	38.0	35.0	40.0	30.0	41.0
40	35.94447475113359	38.0	35.0	40.0	30.0	41.0
41	35.86928894688377	38.0	35.0	40.0	30.0	41.0
42	35.73455340790233	38.0	35.0	40.0	29.0	41.0
43	35.64424214123305	37.0	35.0	40.0	29.0	41.0
44	35.57479739372172	37.0	35.0	40.0	29.0	41.0
45	35.548290920940275	37.0	34.0	40.0	29.0	41.0
46	35.489089591907096	37.0	34.0	40.0	29.0	41.0
47	35.40189797459184	37.0	34.0	40.0	29.0	41.0
48	35.33564488512795	37.0	34.0	40.0	29.0	41.0
49	35.26808036461129	36.0	34.0	40.0	28.0	41.0
50	35.17784110663542	36.0	34.0	40.0	28.0	41.0
51	35.000796459790784	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	10.0
11	6.0
12	30.0
13	33.0
14	31.0
15	44.0
16	98.0
17	190.0
18	384.0
19	764.0
20	1300.0
21	2126.0
22	3201.0
23	4752.0
24	6933.0
25	9763.0
26	13299.0
27	16466.0
28	18912.0
29	22590.0
30	27261.0
31	33690.0
32	42040.0
33	54881.0
34	91286.0
35	175244.0
36	101824.0
37	138408.0
38	203771.0
39	405262.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.7704682892625585	91.33022604910848	3.1517986898781962	3.7475069717507714
2	3.4327780662974585	2.4640792997554612	91.08066864799677	3.022473985950304
3	27.371449935046705	15.703204896009263	44.249778518715715	12.675566650228317
4	27.239215788960703	15.071710475591962	44.12670911542775	13.562364620019581
5	24.898205892493202	13.948083914130724	47.13078087972802	14.022929313648048
6	22.275561995120867	14.127014606854354	47.53766636553941	16.05975703248538
7	23.105844050990886	16.975576687803766	44.890510417985006	15.028068843220346
8	25.735034193218965	19.119617350167363	43.30086395884885	11.844484497764821
9	81.50889489203787	4.250913201157376	9.864754581280359	4.375437325524391
10	82.35117839681008	4.313029791232978	9.42870193783395	3.9070898741229847
11	75.99753861193425	6.701609066985542	10.757153227225977	6.54369909385424
12	44.733036861177425	22.30298348746103	15.915375965389275	17.048603685972267
13	34.79045470216768	23.59637599884786	23.59179362744884	18.02137567153562
14	30.587329088457228	22.726234585411767	27.16240651598666	19.524029810144352
15	22.034296504159776	28.215988257491446	30.116072194897715	19.633643043451062
16	17.819969538140604	32.84716554871352	29.942451234112628	19.39041367903325
17	18.250712449648468	23.35343757864586	38.678560466209014	19.717289505496662
18	21.22059826859097	21.041303895597576	36.365626686567246	21.3724711492442
19	20.880120800038405	21.376689840373455	29.62488562255516	28.118303737032978
20	22.803916691033244	21.823216475589053	31.98669803045313	23.38616880292457
21	26.29139226990313	25.185295097444495	26.79952634281666	21.72378628983572
22	28.270322089794114	22.552395416464822	26.227166334262904	22.950116159478164
23	26.130136438290002	25.112704515599702	27.293840565479176	21.463318480631116
24	27.869691904622666	20.833133309184966	25.555958028387426	25.741216757804942
25	27.31922544830867	24.35544945789819	23.75915928759399	24.566165806199148
26	25.401466649791903	20.65929414023802	30.831576757630373	23.1076624523397
27	26.61084901886337	22.477113600623785	26.832257567095375	24.079779813417474
28	22.45492910416821	28.083463167189638	25.860794830503174	23.600812898138976
29	21.8703494385504	26.386894708743018	28.222025349969527	23.52073050273706
30	22.58767240263188	25.822826610339867	30.243942177746554	21.3455588092817
31	21.79826800908328	25.25373972421398	29.43679018703349	23.511202079669253
32	21.910717948494145	25.887852642573577	30.47902510412166	21.722404304810617
33	27.0381005997815	24.599478918909483	25.962043417605322	22.40037706370369
34	26.50967316781517	26.832621247365136	25.54737517402101	21.110330410798685
35	24.912462159067932	25.092120212331086	25.356733976610997	24.638683651989986
36	21.869040189579252	24.567184110954486	28.143761355916425	25.42001434354984
37	23.651291719582147	25.138380342645	28.461399703527846	22.748928234245007
38	25.570287031016107	27.538379178868137	23.929798070167017	22.961535719948735
39	23.883392467745196	28.77351011103886	26.036670608960794	21.306426812255154
40	23.430465059781763	26.760467081844062	27.63489992246337	22.17416793591081
41	22.365245549644538	27.273547206426375	25.45921907663034	24.901988167298743
42	24.234707608336716	23.9333621368107	27.210194103433576	24.621736151419007
43	20.903614545465125	24.034247043643088	27.88620298886993	27.17593542202186
44	22.094231012616795	25.242829316121075	25.150672735763006	27.512266935499124
45	21.85391109035709	23.299249218451102	28.047749764698864	26.799089926492943
46	23.74584858972065	23.687150594180824	28.068479540075387	24.49852127602314
47	21.140515873189052	26.601538803957425	27.130839068571188	25.12710625428233
48	21.530381122375502	26.762358219246835	26.43635522543085	25.270905432946815
49	22.580544269344518	25.570505239177965	28.905162368693237	22.943788122784277
50	22.77765897555632	22.367282159155213	31.68193396439134	23.173124900897125
51	22.26923395842698	22.8661060171628	28.22253450234719	26.642125522063026
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	718.0
1	1417.5
2	2117.0
3	4514.5
4	6912.0
5	4890.5
6	2869.0
7	2750.0
8	2631.0
9	2654.5
10	2678.0
11	2842.5
12	3007.0
13	3282.5
14	3558.0
15	3641.5
16	3725.0
17	3429.0
18	3133.0
19	3062.5
20	2992.0
21	3588.5
22	4185.0
23	6208.5
24	8232.0
25	8305.5
26	11510.5
27	14642.0
28	14895.0
29	15148.0
30	20040.5
31	24933.0
32	28372.5
33	31812.0
34	33629.0
35	35446.0
36	39240.5
37	43035.0
38	51368.0
39	59701.0
40	78074.5
41	96448.0
42	112506.5
43	128565.0
44	131749.0
45	134933.0
46	128913.5
47	122894.0
48	105850.0
49	88806.0
50	84074.0
51	79342.0
52	77030.5
53	74719.0
54	75350.0
55	75981.0
56	74473.5
57	72966.0
58	66198.5
59	59431.0
60	54592.5
61	49754.0
62	43389.5
63	37025.0
64	32197.5
65	27370.0
66	21070.0
67	14770.0
68	12566.5
69	10363.0
70	9615.0
71	8867.0
72	7373.5
73	5880.0
74	4476.0
75	2545.5
76	2019.0
77	1476.5
78	934.0
79	699.0
80	464.0
81	362.0
82	260.0
83	157.5
84	55.0
85	44.0
86	33.0
87	24.0
88	15.0
89	11.0
90	7.0
91	5.0
92	3.0
93	2.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1374834.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.018581542631992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.29244147834689	11.58022368736851
2	11.470021733370256	3.674669568635052
3	4.672957949968662	2.2456247390059034
4	2.461284991348584	1.5770517733429432
5	1.4089755231298267	1.128489465441385
6	0.925644766064268	0.8896509698826598
7	0.659414912180228	0.7394024078830548
8	0.4465547653358664	0.5722539137426778
9	0.31106835544649153	0.4484586435346852
>10	2.561324770775469	9.09720969681882
>50	0.9665782350750619	11.456627641800482
>100	1.8054951937688104	52.94214211715127
>500	0.010942395113750366	1.1140609436306477
>1k	0.006838996946093979	2.080447098483193
>5k	4.5593312973959857E-4	0.45368733327871924
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	6212	0.4518363671541437	TruSeq Adapter, Index 10 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3766	0.2739239791858508	No Hit
ATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	2912	0.21180738911024893	TruSeq Adapter, Index 10 (95% over 22bp)
ATGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	2536	0.1844586328240355	TruSeq Adapter, Index 10 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	2252	0.16380159350147	TruSeq Adapter, Index 10 (95% over 24bp)
ATGCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	2063	0.15005447930441057	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2005	0.14583578817515425	RNA PCR Primer, Index 10 (95% over 23bp)
ATTGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1968	0.1431445541789045	No Hit
ATGTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1743	0.12677894203954804	No Hit
ATATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1612	0.11725051897174496	No Hit
ATGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1528	0.11114069043971855	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17522115397204316	0.0
2	0.0	0.0	0.0	0.8315913048411663	0.0
3	0.0	0.0	0.0	1.1373736756582977	0.0
4	7.273605395269538E-5	0.0	0.0	1.510218688219814	0.0
5	7.273605395269538E-5	0.0	0.0	2.8501622741363684	0.0
6	7.273605395269538E-5	0.0	0.0	3.148234623234514	0.0
7	7.273605395269538E-5	0.0	0.0	3.6567323764178075	0.0
8	7.273605395269538E-5	0.0	0.0	5.148112426663873	0.0
9	7.273605395269538E-5	0.0	0.0	5.353300834864427	0.0
10	1.4547210790539076E-4	0.0	0.0	5.739383809245334	0.0
11	1.4547210790539076E-4	0.0	0.0	6.017962895884158	0.0
12	1.4547210790539076E-4	0.0	0.0	6.787946763027391	0.0
13	1.4547210790539076E-4	0.0	0.0	7.445844371029521	0.0
14	2.1820816185808613E-4	0.0	0.0	7.73860698818912	0.0
15	2.1820816185808613E-4	0.0	0.0	7.893971199432077	0.0
16	2.9094421581078153E-4	0.0	0.0	7.988600805624533	0.0
17	2.9094421581078153E-4	0.0	0.0	8.120907687764486	0.0
18	2.9094421581078153E-4	0.0	0.0	8.420871174265402	0.0
19	2.9094421581078153E-4	0.0	0.0	8.81830097306293	0.0
20	2.9094421581078153E-4	0.0	0.0	9.293267405374031	0.0
21	2.9094421581078153E-4	0.0	0.0	9.577738112383022	0.0
22	3.636802697634769E-4	0.0	0.0	9.891594185188902	0.0
23	3.636802697634769E-4	0.0	0.0	10.344448857098385	0.0
24	4.3641632371617227E-4	0.0	0.0	10.728277013806757	0.0
25	5.091523776688677E-4	0.0	0.0	11.128470782654487	0.0
26	5.091523776688677E-4	0.0	0.0	11.456219441765333	0.0
27	5.091523776688677E-4	0.0	0.0	11.755891984050438	0.0
28	5.091523776688677E-4	0.0	0.0	12.031052476153485	0.0
29	5.818884316215631E-4	0.0	0.0	12.281846390182379	0.0
30	5.818884316215631E-4	0.0	0.0	12.541368630685595	0.0
31	5.818884316215631E-4	0.0	0.0	12.823511783968101	0.0
32	5.818884316215631E-4	0.0	0.0	13.145514294816683	0.0
33	6.546244855742585E-4	0.0	0.0	13.447296182666417	0.0
34	7.273605395269538E-4	0.0	0.0	13.754387802454696	0.0
35	8.000965934796491E-4	0.0	0.0	14.050205333880308	0.0
36	8.000965934796491E-4	0.0	0.0	14.343913519741292	0.0
37	8.000965934796491E-4	0.0	0.0	14.689337039962643	0.0
38	8.000965934796491E-4	0.0	0.0	15.016212866426056	0.0
39	8.728326474323445E-4	0.0	0.0	15.341706707864368	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	55	1.8189894E-12	45.000004	40
TAACGCG	55	1.8189894E-12	45.000004	3
TCGATGT	75	0.0	45.000004	2
AGCTAGT	55	1.8189894E-12	45.000004	16
ATCGCAC	85	0.0	45.000004	1
CGCTTGT	55	1.8189894E-12	45.000004	28
CTTAACG	25	3.8912065E-5	45.0	4
AACCGTA	45	3.8562575E-10	45.0	26
AGCCGTC	20	7.03377E-4	45.0	37
AACCGGT	25	3.8912065E-5	45.0	33
CGTATGA	40	6.8175723E-9	45.0	11
CTACTCG	25	3.8912065E-5	45.0	26
GTCGAAG	20	7.03377E-4	45.0	35
GTCGAAA	20	7.03377E-4	45.0	12
GGTCGAA	20	7.03377E-4	45.0	11
TCCGATT	25	3.8912065E-5	45.0	30
CTATCGA	25	3.8912065E-5	45.0	38
TCCGACA	40	6.8175723E-9	45.0	22
TAGGTCG	35	1.2121563E-7	45.0	2
GGCGTAA	25	3.8912065E-5	45.0	9
>>END_MODULE