FastQCFastQC Report
Sat 18 Jun 2016
SRR3549073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853500
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC47620.2569193417858106No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38630.208416509306717No Hit
ATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG27260.1470731049366064No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC23700.1278661990828163No Hit
ATGCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTC21580.1164283787429188No Hit
ATGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT18750.10115996762881035No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT18630.10051254383598596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAG453.8562575E-1045.0000043
AACGTCC253.8917336E-545.043
GTACCTA253.8917336E-545.021
ACCTCGT253.8917336E-545.045
CGTTAGT253.8917336E-545.014
AACGGTT253.8917336E-545.040
TACGCTA207.0344075E-445.02
CATCGAA406.8193913E-945.032
CAGTCGA351.2124656E-745.013
TTCGCAT551.8189894E-1245.02
CAATCGA351.2124656E-745.023
TTCGATC351.2124656E-745.019
TCGGTAA207.0344075E-445.02
TAGTCGA207.0344075E-445.01
AGTCGAC406.8193913E-945.035
TAATCGC302.1662254E-644.99999616
ACAACGA850.042.3529415
AGTACGA850.042.3529436
ATTCGCA700.041.7857131
CGTACAT700.041.78571336