##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549073_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1853500
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.908669004585917	31.0	31.0	34.0	31.0	34.0
2	32.54384731588886	34.0	31.0	34.0	31.0	34.0
3	32.52134016725115	34.0	31.0	34.0	31.0	34.0
4	36.00641920690585	37.0	35.0	37.0	35.0	37.0
5	35.792407337469655	37.0	35.0	37.0	35.0	37.0
6	35.78944105745886	37.0	35.0	37.0	35.0	37.0
7	35.778002697599135	37.0	35.0	37.0	35.0	37.0
8	35.74794712705692	37.0	35.0	37.0	33.0	37.0
9	37.90812247100081	39.0	38.0	39.0	35.0	39.0
10	37.84757539789587	39.0	38.0	39.0	35.0	39.0
11	37.675478823846774	39.0	38.0	39.0	35.0	39.0
12	37.18963960075533	39.0	37.0	39.0	33.0	39.0
13	36.92043916913946	39.0	37.0	39.0	33.0	39.0
14	37.6863965470731	40.0	36.0	41.0	33.0	41.0
15	37.63006959805773	40.0	36.0	41.0	33.0	41.0
16	37.669214459131375	40.0	36.0	41.0	33.0	41.0
17	37.73357054221743	40.0	36.0	41.0	33.0	41.0
18	37.767861882924194	40.0	36.0	41.0	33.0	41.0
19	37.70091826274616	40.0	35.0	41.0	33.0	41.0
20	37.675790126787156	40.0	36.0	41.0	33.0	41.0
21	37.6609803075263	40.0	36.0	41.0	33.0	41.0
22	37.57897275424872	40.0	35.0	41.0	33.0	41.0
23	37.517827893175074	40.0	35.0	41.0	32.0	41.0
24	37.485177771783114	40.0	35.0	41.0	32.0	41.0
25	37.40961694092258	39.0	35.0	41.0	32.0	41.0
26	37.28813973563528	39.0	35.0	41.0	32.0	41.0
27	37.19672889128675	39.0	35.0	41.0	32.0	41.0
28	37.09331642837874	39.0	35.0	41.0	31.0	41.0
29	37.03948961424332	39.0	35.0	41.0	31.0	41.0
30	36.97662098732128	39.0	35.0	41.0	31.0	41.0
31	36.87543728082007	39.0	35.0	41.0	31.0	41.0
32	36.759989209603454	39.0	35.0	41.0	31.0	41.0
33	36.63506986781764	39.0	35.0	41.0	31.0	41.0
34	36.55250229295927	39.0	35.0	41.0	30.0	41.0
35	36.46543512274076	39.0	35.0	41.0	30.0	41.0
36	36.374746695441054	38.0	35.0	41.0	30.0	41.0
37	36.21976962503372	38.0	35.0	41.0	30.0	41.0
38	36.13990072835177	38.0	35.0	41.0	30.0	41.0
39	36.111690855138924	38.0	35.0	40.0	30.0	41.0
40	36.008796331265174	38.0	35.0	40.0	30.0	41.0
41	35.93667224170488	38.0	35.0	40.0	30.0	41.0
42	35.798063663339626	38.0	35.0	40.0	29.0	41.0
43	35.674284326949014	38.0	35.0	40.0	29.0	41.0
44	35.58697599136768	37.0	34.0	40.0	29.0	41.0
45	35.54105206366334	37.0	34.0	40.0	29.0	41.0
46	35.47864958187213	37.0	34.0	40.0	28.0	41.0
47	35.3768993795522	37.0	34.0	40.0	28.0	41.0
48	35.28960507148638	37.0	34.0	40.0	28.0	41.0
49	35.254467763690315	37.0	34.0	40.0	28.0	41.0
50	35.14267925546264	36.0	34.0	40.0	28.0	41.0
51	34.94747720528729	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	12.0
11	24.0
12	29.0
13	29.0
14	44.0
15	63.0
16	126.0
17	290.0
18	553.0
19	1103.0
20	1843.0
21	2981.0
22	4464.0
23	6522.0
24	9312.0
25	12994.0
26	17595.0
27	21600.0
28	25684.0
29	30400.0
30	37283.0
31	45420.0
32	56916.0
33	73786.0
34	118634.0
35	211017.0
36	144739.0
37	197703.0
38	287553.0
39	544507.0
40	265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.3599136768276234	91.19789587267331	3.224008632317238	3.218181818181818
2	3.255948206096574	2.858483949285136	91.02497976800647	2.8605880766118155
3	28.572052872943082	16.20766118154842	41.79892096034529	13.421364985163207
4	27.71211222012409	15.506824925816023	43.145886161316426	13.635176692743459
5	25.085999460480174	14.409927164823308	45.75025627191799	14.753817102778527
6	23.035554356622605	14.6	47.043593202050175	15.320852441327219
7	22.458483949285135	18.000161855948207	43.86905853790127	15.67229565686539
8	26.77485837604532	20.1583490693283	41.96185594820609	11.104936606420287
9	83.92775829511734	4.447154032910709	8.022066360938766	3.6030213110331806
10	84.69225789047748	4.284434852980847	7.533531157270029	3.4897760992716487
11	78.10477475047209	6.4176962503372	9.055246830321014	6.422282168869706
12	45.267386026436476	22.814459131373077	15.020285945508496	16.897868896681953
13	34.68605341246291	24.65087671971945	22.0318856217966	18.63118424602104
14	28.63140005395198	23.3035878068519	27.566387914755868	20.49862422444025
15	23.518964121931482	26.004370110601567	29.87995683841381	20.596708929053143
16	20.27191799298624	29.178635014836797	30.078068519018075	20.47137847315889
17	19.23668734826005	22.37734016725115	36.94890747234961	21.437065012139193
18	23.89571081737254	20.49765308875101	33.68686269220394	21.91977340167251
19	23.109738332883733	21.230914486107363	29.00733746965201	26.652009711356893
20	24.3743728082007	21.660480172646345	30.756137038036147	23.209009981116807
21	27.695225249527923	24.06204478014567	26.2123550040464	22.03037496628001
22	28.48206096574049	22.12959266253035	26.329646614513084	23.05869975721608
23	26.942702994335043	24.166873482600483	26.125546263825193	22.764877259239277
24	29.045913137307792	21.312813595899648	25.154680334502295	24.48659293229026
25	28.921985432964664	22.951928783382787	24.34016725114648	23.78591853250607
26	27.612732667925542	20.67369840841651	28.649096304289184	23.06447261936876
27	28.474723496088483	22.239169139465876	25.42017804154303	23.865929322902616
28	24.63577016455355	26.68357162125708	23.75473428648503	24.925923927704343
29	23.784677636903158	25.97658483949285	25.71443215538171	24.524305368222283
30	23.260480172646343	25.073590504451037	27.353007823037494	24.31292149986512
31	24.003237118964122	23.959643916913947	26.299919072025897	25.737199892096037
32	24.35505799838144	26.536120852441325	25.384192069058535	23.724629080118696
33	27.272241704882656	25.93320744537362	24.152899919072027	22.6416509306717
34	28.840463987051525	24.135419476665767	23.339034259509038	23.685082276773674
35	27.171027785271107	26.405341246290803	23.17286215268411	23.25076881575398
36	25.258969517129753	25.631777717831127	24.491340706770973	24.61791205826814
37	25.340113299163747	24.880820070137577	26.863501483679524	22.915565147019155
38	26.328028055031023	27.751443215538167	22.47019152953871	23.450337199892097
39	26.908011869436198	27.785055300782304	23.794011329916376	21.51292149986512
40	25.020285945508498	26.630374966280012	24.288319395737794	24.0610196924737
41	25.387537091988133	27.301429727542487	23.01246290801187	24.29857027245751
42	25.768060426220664	24.017588346371728	25.87332074453736	24.341030482870245
43	23.231723765848393	24.62292959266253	26.0079309414621	26.137415700026978
44	23.93676827623415	25.79589964931211	23.984677636903157	26.28265443755058
45	23.108173725384408	23.638036147828434	26.43285675748584	26.82093336930132
46	24.929376854599404	24.411599676288105	25.337739411923387	25.321284057189104
47	23.523927704343134	26.334286485028326	24.53245211761532	25.60933369301322
48	24.30725654167791	25.633558133261396	24.323819800377663	25.735365524683036
49	24.134772052872943	24.92446722417049	26.812624763960073	24.128135958996495
50	23.400539519827355	23.52133800917184	27.8132721877529	25.26485028324791
51	23.44353925006744	24.38532506069598	26.910817372538443	25.26031831669814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	663.0
1	1503.5
2	2344.0
3	4795.0
4	7246.0
5	5173.0
6	3100.0
7	3134.5
8	3169.0
9	3231.0
10	3293.0
11	3405.0
12	3517.0
13	3697.0
14	3877.0
15	3941.0
16	4005.0
17	4074.0
18	4143.0
19	4444.5
20	4746.0
21	4637.0
22	4528.0
23	5392.5
24	6257.0
25	7471.0
26	11341.5
27	13998.0
28	17295.0
29	20592.0
30	24566.5
31	28541.0
32	30569.0
33	32597.0
34	37051.0
35	41505.0
36	47314.5
37	53124.0
38	65471.5
39	77819.0
40	87758.0
41	97697.0
42	108656.5
43	119616.0
44	123205.0
45	126794.0
46	136069.0
47	145344.0
48	153163.5
49	160983.0
50	150125.0
51	139267.0
52	133171.0
53	127075.0
54	124104.0
55	121133.0
56	118481.5
57	115830.0
58	110785.5
59	105741.0
60	93412.0
61	81083.0
62	73687.0
63	66291.0
64	53610.5
65	40930.0
66	34296.5
67	27663.0
68	22457.5
69	17252.0
70	15506.0
71	13760.0
72	11244.0
73	8728.0
74	6786.0
75	4350.0
76	3856.0
77	2466.0
78	1076.0
79	844.5
80	613.0
81	342.0
82	71.0
83	55.5
84	40.0
85	32.0
86	24.0
87	19.5
88	15.0
89	13.0
90	11.0
91	10.0
92	9.0
93	7.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1853500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.046750073158105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.69071388055649	11.824964683538928
2	10.485653049522691	3.3652130767907753
3	4.148992941120755	1.9973355834438586
4	2.174317422253576	1.3956291301846606
5	1.2942950895692593	1.0384614911616845
6	0.7929556143904362	0.7634616337938517
7	0.5781479645996987	0.6494177125265499
8	0.4174183611310325	0.5358566493613546
9	0.29484212071688615	0.42581320399654066
>10	2.867192295972591	11.071182620260739
>50	1.4461630910137797	17.08077290526891
>100	1.7968092142874654	47.365882950674035
>500	0.006756191819090759	0.6884347519521316
>1k	0.005742763046227143	1.7975736070460342
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	4762	0.2569193417858106	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3863	0.208416509306717	No Hit
ATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG	2726	0.1470731049366064	No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	2370	0.1278661990828163	No Hit
ATGCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTC	2158	0.1164283787429188	No Hit
ATGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	1875	0.10115996762881035	No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	1863	0.10051254383598596	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11033180469382249	0.0
2	0.0	0.0	0.0	0.49420016185594823	0.0
3	0.0	0.0	0.0	0.7018613434043701	0.0
4	0.0	0.0	0.0	0.92176962503372	0.0
5	0.0	0.0	0.0	1.7585648772592393	0.0
6	0.0	0.0	0.0	1.9574858376045319	0.0
7	0.0	0.0	0.0	2.3148098192608577	0.0
8	0.0	0.0	0.0	3.3180469382249798	0.0
9	0.0	0.0	0.0	3.44575128135959	0.0
10	0.0	0.0	0.0	3.6830860534124628	0.0
11	0.0	0.0	0.0	3.8700296735905044	0.0
12	0.0	0.0	0.0	4.454653358510925	0.0
13	0.0	0.0	0.0	4.961154572430537	0.0
14	5.395198273536552E-5	0.0	0.0	5.170865929322902	0.0
15	5.395198273536552E-5	0.0	0.0	5.293498786080389	0.0
16	5.395198273536552E-5	0.0	0.0	5.3773401672511465	0.0
17	5.395198273536552E-5	0.0	0.0	5.482762341516051	0.0
18	5.395198273536552E-5	0.0	0.0	5.72689506339358	0.0
19	1.0790396547073105E-4	0.0	0.0	6.026274615592123	0.0
20	1.0790396547073105E-4	0.0	0.0	6.365740490963043	0.0
21	1.0790396547073105E-4	0.0	0.0	6.57928243862962	0.0
22	2.158079309414621E-4	0.0	0.0	6.823415160507149	0.0
23	2.158079309414621E-4	0.0	0.0	7.194658753709199	0.0
24	2.158079309414621E-4	0.0	0.0	7.533207445373617	0.0
25	2.158079309414621E-4	0.0	0.0	7.89813865659563	0.0
26	2.158079309414621E-4	0.0	0.0	8.176854599406528	0.0
27	2.158079309414621E-4	0.0	0.0	8.436579444294578	0.0
28	2.697599136768276E-4	0.0	0.0	8.666037226868088	0.0
29	2.697599136768276E-4	0.0	0.0	8.880334502292959	0.0
30	2.697599136768276E-4	0.0	0.0	9.114324251416239	0.0
31	2.697599136768276E-4	0.0	0.0	9.36471540329107	0.0
32	2.697599136768276E-4	0.0	0.0	9.632425141623955	0.0
33	2.697599136768276E-4	0.0	0.0	9.899001888319395	0.0
34	2.697599136768276E-4	0.0	0.0	10.152630159158349	0.0
35	3.2371189641219314E-4	0.0	0.0	10.410898300512544	0.0
36	3.2371189641219314E-4	0.0	0.0	10.670569193417858	0.0
37	3.7766387914755866E-4	0.0	0.0	10.967035338548692	0.0
38	3.7766387914755866E-4	0.0	0.0	11.247531696789856	0.0
39	3.7766387914755866E-4	0.0	0.0	11.52818991097923	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAG	45	3.8562575E-10	45.000004	3
AACGTCC	25	3.8917336E-5	45.0	43
GTACCTA	25	3.8917336E-5	45.0	21
ACCTCGT	25	3.8917336E-5	45.0	45
CGTTAGT	25	3.8917336E-5	45.0	14
AACGGTT	25	3.8917336E-5	45.0	40
TACGCTA	20	7.0344075E-4	45.0	2
CATCGAA	40	6.8193913E-9	45.0	32
CAGTCGA	35	1.2124656E-7	45.0	13
TTCGCAT	55	1.8189894E-12	45.0	2
CAATCGA	35	1.2124656E-7	45.0	23
TTCGATC	35	1.2124656E-7	45.0	19
TCGGTAA	20	7.0344075E-4	45.0	2
TAGTCGA	20	7.0344075E-4	45.0	1
AGTCGAC	40	6.8193913E-9	45.0	35
TAATCGC	30	2.1662254E-6	44.999996	16
ACAACGA	85	0.0	42.35294	15
AGTACGA	85	0.0	42.35294	36
ATTCGCA	70	0.0	41.785713	1
CGTACAT	70	0.0	41.785713	36
>>END_MODULE