FastQCFastQC Report
Sat 18 Jun 2016
SRR3549070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188776
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTT83680.7039173065405089TruSeq Adapter, Index 14 (95% over 24bp)
TTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC69030.5806813058137109TruSeq Adapter, Index 14 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC63410.5334057888113488TruSeq Adapter, Index 15 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC53160.44718264837109767TruSeq Adapter, Index 14 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC51400.432377504256479TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT41180.3464067242272724TruSeq Adapter, Index 15 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC38300.3221801247669872TruSeq Adapter, Index 15 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC37770.3177217575051986TruSeq Adapter, Index 15 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC30790.25900590186881295TruSeq Adapter, Index 14 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC25590.21526343062107578TruSeq Adapter, Index 14 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC25510.21459046952495678TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT23640.19886000390317438TruSeq Adapter, Index 15 (95% over 22bp)
CTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC22800.19179391239392451TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT17320.14569607730977072TruSeq Adapter, Index 15 (95% over 22bp)
TACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC13570.11415102592919103TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGCG750.046.0000041
ACGTAGG750.046.0000042
TCGCCGA301.8614573E-646.043
TCGCCCA206.311542E-446.033
ACAACGG206.311542E-446.036
CTTCGCG551.8189894E-1246.01
AACGTAT206.311542E-446.011
AACGTAG206.311542E-446.024
CGGCGTG206.311542E-446.020
AACAACG206.311542E-446.035
CGGCGAA405.6115823E-946.05
AATCCGT206.311542E-446.031
GTACCGA206.311542E-446.033
ACAGTCG206.311542E-446.044
GTACCCC206.311542E-446.027
TACCCGA301.8614573E-646.012
TCGCAAG253.417227E-546.01
AAATCCG501.6370905E-1146.034
CGAACCG405.6115823E-946.027
CGAACAC206.311542E-446.012
Produced by FastQC (version 0.11.5)