##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549070_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1188776
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58962748238525	33.0	31.0	34.0	31.0	34.0
2	32.97009108528436	34.0	31.0	34.0	31.0	34.0
3	33.02835521578498	34.0	33.0	34.0	31.0	34.0
4	36.40450934406482	37.0	37.0	37.0	35.0	37.0
5	36.383510434261794	37.0	37.0	37.0	35.0	37.0
6	36.381576512311824	37.0	37.0	37.0	35.0	37.0
7	36.51001954951984	37.0	37.0	37.0	35.0	37.0
8	36.57445220966776	37.0	37.0	37.0	35.0	37.0
9	38.38303010827944	39.0	39.0	39.0	37.0	39.0
10	38.0952702611762	39.0	39.0	39.0	35.0	39.0
11	37.89639175084288	39.0	38.0	39.0	35.0	39.0
12	37.53913100533658	39.0	37.0	39.0	35.0	39.0
13	37.45052221781059	39.0	37.0	39.0	35.0	39.0
14	38.748339468495324	41.0	38.0	41.0	35.0	41.0
15	38.8353903510838	41.0	38.0	41.0	35.0	41.0
16	38.90211107895853	41.0	38.0	41.0	35.0	41.0
17	38.890496611640884	41.0	38.0	41.0	35.0	41.0
18	38.87895701124518	41.0	38.0	41.0	35.0	41.0
19	38.87243685942516	40.0	38.0	41.0	35.0	41.0
20	38.803730055115516	40.0	38.0	41.0	35.0	41.0
21	38.768838704684484	40.0	38.0	41.0	35.0	41.0
22	38.824321823455385	41.0	38.0	41.0	35.0	41.0
23	38.83442212830676	41.0	38.0	41.0	35.0	41.0
24	38.80914907434201	41.0	38.0	41.0	35.0	41.0
25	38.69591243430217	40.0	37.0	41.0	35.0	41.0
26	38.57344613282906	40.0	37.0	41.0	35.0	41.0
27	38.43358715182675	40.0	37.0	41.0	35.0	41.0
28	38.50433723426449	40.0	37.0	41.0	35.0	41.0
29	38.47551683412182	40.0	37.0	41.0	35.0	41.0
30	38.384873180481435	40.0	36.0	41.0	35.0	41.0
31	38.42029701137977	40.0	36.0	41.0	35.0	41.0
32	38.38753894762344	40.0	36.0	41.0	35.0	41.0
33	38.29003529680949	40.0	36.0	41.0	35.0	41.0
34	38.23355619561633	40.0	36.0	41.0	35.0	41.0
35	38.20429584715708	40.0	36.0	41.0	34.0	41.0
36	38.1065844196047	40.0	36.0	41.0	34.0	41.0
37	38.10007436220112	40.0	36.0	41.0	34.0	41.0
38	38.05800672288135	40.0	36.0	41.0	34.0	41.0
39	38.04103716764134	40.0	36.0	41.0	34.0	41.0
40	37.897253141045915	40.0	35.0	41.0	34.0	41.0
41	37.77636745694731	40.0	35.0	41.0	34.0	41.0
42	37.7559514996938	40.0	35.0	41.0	34.0	41.0
43	37.72900445500245	40.0	35.0	41.0	34.0	41.0
44	37.62550640322483	40.0	35.0	41.0	33.0	41.0
45	37.531063884196854	40.0	35.0	41.0	33.0	41.0
46	37.54075788878645	40.0	35.0	41.0	33.0	41.0
47	37.54410923504512	40.0	35.0	41.0	33.0	41.0
48	37.47760217231842	39.0	35.0	41.0	33.0	41.0
49	37.473572817755404	39.0	35.0	41.0	33.0	41.0
50	37.40971553934467	39.0	35.0	41.0	33.0	41.0
51	37.28330232104282	39.0	35.0	41.0	33.0	41.0
52	36.948631197130496	39.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	3.0
13	5.0
14	12.0
15	8.0
16	9.0
17	34.0
18	85.0
19	148.0
20	268.0
21	470.0
22	871.0
23	1416.0
24	2135.0
25	2886.0
26	3697.0
27	4707.0
28	6195.0
29	8139.0
30	10494.0
31	13620.0
32	18219.0
33	25076.0
34	54273.0
35	142562.0
36	57805.0
37	78342.0
38	146968.0
39	609571.0
40	756.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.88828845804424	13.805292166059878	48.836702625221236	12.469716750674644
2	22.94200084793098	13.102552541437579	52.80145292300652	11.153993687624919
3	23.706064052437128	13.980766771872918	47.00692140487359	15.30624777081637
4	19.76335323054974	13.945352194189653	52.10148926290571	14.189805312354892
5	22.54150487560314	15.898621775675148	47.543607879028514	14.016265469693199
6	21.865767814962616	17.83431024852453	47.31412814525192	12.985793791260928
7	84.47975060061778	1.7017503718110056	7.764709247158422	6.053789780412795
8	87.05794867998681	1.7410344757969543	7.858082599244938	3.3429342449712984
9	78.23509222931823	4.268003391723925	9.421455345666468	8.075449033291385
10	39.59846093797317	29.430691736710703	13.786869856053622	17.1839774692625
11	30.282576364260382	22.103491322166665	32.428733419920995	15.185198893651958
12	29.351366447505672	26.125190952711026	26.32741576209479	18.196026837688514
13	20.273373621270956	29.14653391387444	35.19906189223201	15.381030572622597
14	14.325491093359894	39.23640786826114	29.671948289669377	16.766152748709597
15	13.198870098319615	27.394227339717492	39.81986513859634	19.587037423366553
16	16.071656897514757	25.315029913120725	39.9078548019139	18.705458387450623
17	15.972226895563168	24.19841921438522	29.934571357429828	29.89478253262179
18	16.77910724980989	25.969905179781556	33.29685323391455	23.954134336494008
19	21.69853698257704	27.740802304218793	31.728265038998092	18.832395674206072
20	24.040441597071275	23.53504781388588	27.24525057706414	25.17926001197871
21	20.617004380976738	29.039617219728527	31.467576734388985	18.875801664905755
22	25.27103508146194	22.938131321628298	26.579187332180325	25.21164626472944
23	22.05688876626042	29.017493623693614	22.8414772841982	26.08414032584776
24	21.337661594783206	22.29856591990417	32.538257838314365	23.825514646998258
25	24.602111751919622	27.21370552568356	27.11166779948451	21.072514922912305
26	18.788989683506397	30.39891451375196	26.647324643162378	24.164771159579264
27	17.93668445527164	31.443770735613775	25.507328546336737	25.11221626277785
28	18.65195798030916	26.331621768945535	30.323374630712596	24.693045620032706
29	16.620120190851768	23.575677840064067	29.48520158549634	30.319000383587824
30	18.378062814188713	30.213177251223104	29.23174761267051	22.17701232191767
31	19.12252602677039	26.80723702362766	23.487688176746502	30.58254877285544
32	25.042228308781468	24.882820649138274	25.189438548557508	24.88551249352275
33	20.325107505535104	25.746818576418097	31.582232481140267	22.345841436906532
34	16.735953619521254	25.020104712746555	29.917663209889838	28.326278457842353
35	19.75107169054557	23.715990228604884	31.971372234971096	24.561565845878448
36	20.221976217554865	23.760489781085756	29.17378883826726	26.84374516309212
37	20.975440284797138	25.971503462384838	31.592242777445033	21.46081347537299
38	17.027177533866766	24.63845165111005	34.83583114060176	23.49853967442142
39	19.674354125588	27.39733978478704	27.676198038991366	25.25210805063359
40	24.82519835528308	22.10079947778219	31.6983182702208	21.37568389671393
41	19.366726784524587	23.227168112411423	31.74180838105749	25.6642967220065
42	17.977230361312813	26.938296197096847	27.155830871417326	27.928642570173018
43	18.079688688196935	23.35696548382538	30.675922124941955	27.88742370303573
44	25.241256552958674	24.671342624682868	24.851275597757695	25.236125224600762
45	17.904045842109866	27.178122707726267	27.969440836625235	26.94839061353863
46	17.68516524559715	27.00559230670875	26.20182439753158	29.10741805016252
47	20.487122889425763	23.65475076885805	32.68386979548713	23.174256546229064
48	17.708803004098332	20.399974427478348	37.609019697571284	24.282202870852036
49	18.225048284958646	20.511854209708137	29.614157755540155	31.648939749793065
50	17.314363681635562	22.855357106805656	34.4091738056623	25.421105405896483
51	20.8039193254238	22.863432639959083	29.941553328802062	26.391094705815053
52	18.737255799242245	20.984777620005787	31.606206720189505	28.671759860562464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	433.0
1	324.0
2	215.0
3	179.5
4	144.0
5	152.0
6	160.0
7	274.0
8	388.0
9	511.5
10	635.0
11	716.5
12	798.0
13	891.5
14	1098.5
15	1212.0
16	1306.5
17	1401.0
18	1509.5
19	1618.0
20	1939.5
21	2261.0
22	3253.0
23	4245.0
24	4458.0
25	4671.0
26	6307.5
27	7944.0
28	10654.5
29	13365.0
30	15199.0
31	17033.0
32	21436.0
33	25839.0
34	30605.5
35	35372.0
36	37891.5
37	40411.0
38	44289.0
39	59029.5
40	69892.0
41	80051.5
42	90211.0
43	98737.5
44	107264.0
45	115622.5
46	123981.0
47	143203.0
48	162425.0
49	157445.0
50	152465.0
51	123654.0
52	94843.0
53	75531.0
54	56219.0
55	45183.5
56	34148.0
57	32989.0
58	31830.0
59	26930.0
60	22030.0
61	19758.5
62	17487.0
63	13848.0
64	6957.0
65	3705.0
66	3288.0
67	2871.0
68	1829.0
69	787.0
70	667.5
71	548.0
72	437.0
73	326.0
74	216.0
75	106.0
76	88.5
77	71.0
78	53.5
79	36.0
80	23.5
81	11.0
82	7.0
83	3.0
84	3.0
85	3.0
86	3.0
87	3.0
88	2.5
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1188776.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.838672962825703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.48548868613328	9.738485472591849
2	13.276625789185667	4.20568267849459
3	6.625810397451068	3.14832131996753
4	4.068596772433271	2.5776469478471506
5	2.6804241423046764	2.1227180695813175
6	1.9012663761481987	1.8068121808216846
7	1.3140714958840094	1.4569204071154591
8	1.0130843019694338	1.2836728754133135
9	0.7989542502275765	1.1388937573452778
>10	4.481531328270422	13.477235825144238
>50	0.8229683743035365	9.48295318428977
>100	1.505676160597568	42.545466538842604
>500	0.016469993288117362	1.7949046198508938
>1k	0.006375481272819624	2.5217862938439564
>5k	0.0026564505303415097	2.6984998288503736
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTT	8368	0.7039173065405089	TruSeq Adapter, Index 14 (95% over 24bp)
TTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	6903	0.5806813058137109	TruSeq Adapter, Index 14 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	6341	0.5334057888113488	TruSeq Adapter, Index 15 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	5316	0.44718264837109767	TruSeq Adapter, Index 14 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	5140	0.432377504256479	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	4118	0.3464067242272724	TruSeq Adapter, Index 15 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3830	0.3221801247669872	TruSeq Adapter, Index 15 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3777	0.3177217575051986	TruSeq Adapter, Index 15 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3079	0.25900590186881295	TruSeq Adapter, Index 14 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	2559	0.21526343062107578	TruSeq Adapter, Index 14 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	2551	0.21459046952495678	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	2364	0.19886000390317438	TruSeq Adapter, Index 15 (95% over 22bp)
CTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	2280	0.19179391239392451	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	1732	0.14569607730977072	TruSeq Adapter, Index 15 (95% over 22bp)
TACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1357	0.11415102592919103	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7354623579210886	0.0
2	0.0	0.0	0.0	1.5074328553066347	0.0
3	0.0	0.0	0.0	5.616869788757512	0.0
4	0.0	0.0	0.0	6.426526107525724	0.0
5	0.0	0.0	0.0	7.811059442653621	0.0
6	0.0	0.0	0.0	11.909392518018533	0.0
7	0.0	0.0	0.0	12.35775284830784	0.0
8	0.0	0.0	0.0	13.403029670854728	0.0
9	8.412013701487917E-5	0.0	0.0	14.133949541377014	0.0
10	8.412013701487917E-5	0.0	0.0	16.31796065869432	0.0
11	8.412013701487917E-5	0.0	0.0	18.234974461126402	0.0
12	8.412013701487917E-5	0.0	0.0	18.981372436859424	0.0
13	8.412013701487917E-5	0.0	0.0	19.34140662328311	0.0
14	8.412013701487917E-5	0.0	0.0	19.49593531497944	0.0
15	8.412013701487917E-5	0.0	0.0	19.74055667341871	0.0
16	8.412013701487917E-5	0.0	0.0	20.431098878173852	0.0
17	8.412013701487917E-5	0.0	0.0	21.472169693869997	0.0
18	8.412013701487917E-5	0.0	0.0	22.634037026319508	0.0
19	8.412013701487917E-5	0.0	0.0	23.350992954097325	0.0
20	8.412013701487917E-5	0.0	0.0	24.04818064967664	0.0
21	8.412013701487917E-5	0.0	0.0	25.1599965006023	0.0
22	8.412013701487917E-5	0.0	0.0	26.161614972038468	0.0
23	8.412013701487917E-5	0.0	0.0	27.24028748898026	0.0
24	3.3648054805951666E-4	0.0	0.0	28.03454982267475	0.0
25	4.2060068507439584E-4	0.0	0.0	28.758067121139728	0.0
26	4.2060068507439584E-4	0.0	0.0	29.37046171860805	0.0
27	4.2060068507439584E-4	0.0	0.0	29.959555038123245	0.0
28	4.2060068507439584E-4	0.0	0.0	30.49792391501847	0.0
29	4.2060068507439584E-4	0.0	0.0	31.066491921102042	0.0
30	4.2060068507439584E-4	0.0	0.0	31.75745472654226	0.0
31	4.2060068507439584E-4	0.0	0.0	32.37615833428669	0.0
32	4.2060068507439584E-4	0.0	0.0	32.94371689872609	0.0
33	4.2060068507439584E-4	0.0	0.0	33.477543288222506	0.0
34	4.2060068507439584E-4	0.0	0.0	34.01069671662281	0.0
35	4.2060068507439584E-4	0.0	0.0	34.685171975208114	0.0
36	4.2060068507439584E-4	0.0	0.0	35.28023782445137	0.0
37	4.2060068507439584E-4	0.0	0.0	35.88607105123253	0.0
38	4.2060068507439584E-4	0.0	0.0	36.446142923477595	0.0
39	4.2060068507439584E-4	0.0	0.0	36.99586801886983	0.0
40	4.2060068507439584E-4	0.0	0.0	37.54929440029072	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTGCG	75	0.0	46.000004	1
ACGTAGG	75	0.0	46.000004	2
TCGCCGA	30	1.8614573E-6	46.0	43
TCGCCCA	20	6.311542E-4	46.0	33
ACAACGG	20	6.311542E-4	46.0	36
CTTCGCG	55	1.8189894E-12	46.0	1
AACGTAT	20	6.311542E-4	46.0	11
AACGTAG	20	6.311542E-4	46.0	24
CGGCGTG	20	6.311542E-4	46.0	20
AACAACG	20	6.311542E-4	46.0	35
CGGCGAA	40	5.6115823E-9	46.0	5
AATCCGT	20	6.311542E-4	46.0	31
GTACCGA	20	6.311542E-4	46.0	33
ACAGTCG	20	6.311542E-4	46.0	44
GTACCCC	20	6.311542E-4	46.0	27
TACCCGA	30	1.8614573E-6	46.0	12
TCGCAAG	25	3.417227E-5	46.0	1
AAATCCG	50	1.6370905E-11	46.0	34
CGAACCG	40	5.6115823E-9	46.0	27
CGAACAC	20	6.311542E-4	46.0	12
>>END_MODULE