FastQCFastQC Report
Sat 18 Jun 2016
SRR3549069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536225
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80840.5262249995931586No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39090.2544549138309818No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT33890.22060570554443523No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18060.11756090416442905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCC302.1659816E-645.00000427
CTACGGC302.1659816E-645.00000418
ACCGTAT207.0340285E-445.00000418
TCGACAC302.1659816E-645.00000434
GTCGACA351.2122837E-745.014
CACGTTA453.8562575E-1045.013
TATACGA253.8914208E-545.044
TGTACGC253.8914208E-545.025
CGGTCTA1500.043.50000431
TATAGCG1300.043.269231
CGACGGT1600.042.18750428
TATACGG1200.041.2500042
CGTTTTT55650.041.199461
TCACGAC1750.041.1428625
ACGACGG1750.041.1428627
CCGTCGG501.0822987E-940.520
ACGGGTA2350.040.2127655
AGGGATG40600.040.178576
CGAGGGA6450.040.1162764
CGTATGG1350.040.0000042
Produced by FastQC (version 0.11.5)