##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549069_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1536225
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.439210402122086	31.0	31.0	33.0	30.0	34.0
2	31.793819264756138	31.0	31.0	34.0	30.0	34.0
3	31.899510813845627	31.0	31.0	34.0	30.0	34.0
4	35.60555192110531	37.0	35.0	37.0	33.0	37.0
5	35.48994841250468	37.0	35.0	37.0	33.0	37.0
6	35.55731419551172	37.0	35.0	37.0	33.0	37.0
7	35.9288352943091	37.0	35.0	37.0	35.0	37.0
8	35.952732184413094	37.0	35.0	37.0	35.0	37.0
9	37.64625949974776	39.0	37.0	39.0	35.0	39.0
10	37.06996110595779	39.0	37.0	39.0	33.0	39.0
11	36.73757229572491	39.0	35.0	39.0	32.0	39.0
12	36.123701931683186	38.0	35.0	39.0	32.0	39.0
13	35.83217106869111	38.0	35.0	39.0	31.0	39.0
14	36.915072336409054	39.0	35.0	41.0	31.0	41.0
15	37.07890510830119	39.0	35.0	41.0	32.0	41.0
16	37.24105908965158	39.0	35.0	41.0	32.0	41.0
17	37.186848280688054	39.0	35.0	41.0	32.0	41.0
18	37.131929242135755	38.0	35.0	41.0	32.0	41.0
19	37.07334342300119	38.0	35.0	41.0	32.0	41.0
20	36.91290468518609	38.0	35.0	41.0	32.0	41.0
21	36.7294120327426	38.0	35.0	41.0	31.0	41.0
22	36.68555777962212	38.0	35.0	41.0	31.0	41.0
23	36.6541600351511	38.0	35.0	41.0	31.0	41.0
24	36.59086527038682	38.0	35.0	40.0	31.0	41.0
25	36.507690605217334	38.0	35.0	40.0	31.0	41.0
26	36.42984523751404	38.0	35.0	40.0	31.0	41.0
27	36.39702322250972	38.0	35.0	40.0	31.0	41.0
28	36.3602467086527	38.0	35.0	40.0	31.0	41.0
29	36.35998633012742	38.0	35.0	40.0	31.0	41.0
30	36.277825839313905	38.0	35.0	40.0	31.0	41.0
31	36.12854822698498	38.0	35.0	40.0	30.0	41.0
32	35.961035655584304	38.0	35.0	40.0	30.0	41.0
33	35.77470683005419	38.0	35.0	40.0	30.0	41.0
34	35.569781119302185	38.0	34.0	40.0	29.0	41.0
35	35.4477456101808	38.0	34.0	40.0	29.0	41.0
36	35.27276668456769	38.0	34.0	40.0	27.0	41.0
37	35.21501863333822	37.0	34.0	40.0	27.0	41.0
38	35.226581392699636	37.0	34.0	40.0	27.0	41.0
39	35.174150921902715	37.0	34.0	40.0	27.0	41.0
40	34.982483685658025	37.0	34.0	40.0	26.0	41.0
41	34.968480528568406	37.0	34.0	40.0	26.0	41.0
42	34.88278019170369	37.0	34.0	40.0	26.0	41.0
43	34.82517339582418	37.0	34.0	40.0	26.0	41.0
44	34.74439291119465	37.0	34.0	40.0	26.0	41.0
45	34.71754788523816	36.0	34.0	40.0	26.0	41.0
46	34.677611677976856	36.0	34.0	40.0	26.0	41.0
47	34.6319881527771	36.0	34.0	40.0	26.0	41.0
48	34.61339647512571	36.0	34.0	40.0	26.0	41.0
49	34.51361551856011	36.0	34.0	40.0	26.0	41.0
50	34.40505785285359	36.0	33.0	40.0	25.0	41.0
51	34.27705902455695	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	6.0
14	15.0
15	46.0
16	91.0
17	255.0
18	635.0
19	1259.0
20	2222.0
21	3514.0
22	5358.0
23	8071.0
24	11706.0
25	16460.0
26	22179.0
27	25867.0
28	28340.0
29	31893.0
30	38661.0
31	47518.0
32	59970.0
33	78715.0
34	133320.0
35	202960.0
36	111530.0
37	139834.0
38	202140.0
39	363438.0
40	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.4353854415857	23.387980276326708	27.124802681898814	15.051831600188775
2	31.39507559114062	25.480772673273773	29.815359078260023	13.308792657325588
3	28.7304919526762	24.368370518641473	33.097495484059955	13.80364204462237
4	25.91462839102345	27.719832706797508	31.202460577063906	15.163078325115137
5	22.603329590392033	32.48358801607837	30.179563540496996	14.733518853032596
6	20.47805497241615	42.355774707481004	27.267229735227588	9.898940584875263
7	86.88206480170548	4.223860437110449	7.1367149994304215	1.7573597617536494
8	87.7778971179352	3.647512571400674	6.813650344187863	1.7609399664762648
9	82.89983563605591	5.6512555127015895	8.419209425702615	3.029699425539879
10	45.4083223486143	26.77465866002701	15.850347442594673	11.966671548764015
11	37.11123045126853	23.3205422382789	24.095819297303457	15.472408013149117
12	37.941772852284004	20.77338931471627	27.524158245048742	13.760679587950984
13	22.846588227635927	36.294357922830315	24.27561066901007	16.583443180523684
14	16.428908525769337	37.25163957102638	28.126739247180588	18.192712656023694
15	14.859737343162625	25.01157057071718	42.44475906849583	17.68393301762437
16	16.067893700467053	20.41816791160149	42.059594134973715	21.45434425295774
17	15.9772168790379	21.512083190938828	31.229865416849744	31.280834513173527
18	19.93464499015444	24.546209051408486	34.703965890413194	20.81518006802389
19	26.589789907077417	26.742632101417435	26.155673810802455	20.511904180702697
20	28.927663590945336	22.28182720630116	28.36238181256001	20.42812739019349
21	20.508779638399325	26.84808540415629	30.484662077495155	22.158472879949226
22	22.008364660124656	25.280671776595227	25.918273690377386	26.79268987290273
23	19.802242510048984	28.97999967452684	26.887662939999025	24.33009487542515
24	18.224543930739312	24.114826929648977	39.834724731077806	17.82590440853391
25	18.47600449152956	27.483994857524124	33.10205210825237	20.937948542693942
26	18.469820501554135	31.960227180263306	27.33994043841234	22.230011879770217
27	17.31816628423571	33.849143191915246	27.806864228872723	21.025826294976323
28	15.316962033556283	29.165324089895684	34.853358069293236	20.664355807254797
29	17.130433367508015	23.55898387280509	34.977298247327056	24.333284512359842
30	18.316132077006948	31.353675405620923	31.315334667773275	19.014857849598855
31	24.686357792641054	30.463376133053426	26.003417468144313	18.846848606161206
32	26.268287522986544	26.425751436150303	27.072857166105223	20.23310387475793
33	25.617601588309004	30.666145909616105	24.658692574329933	19.05755992774496
34	19.192045436052663	27.65350127748214	28.474539862324853	24.67991342414034
35	20.749304301127765	26.73683867922993	30.414490064931893	22.099366954710412
36	26.964344415694313	28.82175462578724	25.455190483164902	18.758710475353546
37	19.515956321502383	33.365099513417626	29.207440316359907	17.911503848720077
38	19.087503458152288	30.29562726814106	29.10491627203046	21.511953001676186
39	20.302039089326108	32.740776904424806	27.823984116909955	19.133199889339128
40	22.956207586779282	27.936597829094044	28.621783918371328	20.485410665755342
41	17.89099903985419	27.077869452716886	30.545590652410944	24.48554085501798
42	20.682517209393154	26.877117609725136	25.8515516932741	26.58881348760761
43	20.953506159579486	27.239759800810425	27.802502888574267	24.004231151035818
44	19.02065127178636	29.638822438119416	28.51483343911211	22.825692850982115
45	17.328581425246952	36.739995768848964	23.354651825090723	22.576770980813357
46	19.740923367345278	34.465589350518314	26.31867076762844	19.474816514507967
47	19.723282722257483	30.824911715406273	28.121076014255724	21.33072954808052
48	21.04027730312942	25.832218587771973	31.333235691386353	21.79426841771225
49	20.22373024784781	27.79280378850754	31.226871063809014	20.756594899835637
50	18.485898875490246	32.73478819834334	29.041383911861868	19.737929014304544
51	18.12683688912757	32.15056388224381	26.83858158798353	22.884017640645087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1271.0
1	2261.5
2	3252.0
3	8612.5
4	13973.0
5	10421.0
6	6869.0
7	6932.0
8	6995.0
9	7284.5
10	7574.0
11	7720.0
12	7866.0
13	7699.5
14	7533.0
15	7022.0
16	6511.0
17	6235.0
18	5959.0
19	6462.5
20	6966.0
21	6819.0
22	6672.0
23	7436.5
24	8201.0
25	8810.0
26	13499.0
27	17579.0
28	20663.5
29	23748.0
30	27775.5
31	31803.0
32	38329.0
33	44855.0
34	53949.5
35	63044.0
36	67598.0
37	72152.0
38	74999.5
39	77847.0
40	93226.0
41	108605.0
42	116779.0
43	124953.0
44	133120.5
45	141288.0
46	134825.5
47	128363.0
48	118008.0
49	107653.0
50	100803.5
51	93954.0
52	87176.0
53	80398.0
54	71861.0
55	63324.0
56	59067.0
57	54810.0
58	51993.0
59	49176.0
60	46670.0
61	44164.0
62	38643.5
63	33123.0
64	28222.5
65	23322.0
66	20174.5
67	17027.0
68	14604.5
69	12182.0
70	10494.0
71	8806.0
72	7782.0
73	6758.0
74	5478.0
75	3066.5
76	1935.0
77	1542.5
78	1150.0
79	894.5
80	639.0
81	400.0
82	161.0
83	111.0
84	61.0
85	46.5
86	32.0
87	22.0
88	12.0
89	14.0
90	16.0
91	13.5
92	11.0
93	9.0
94	7.0
95	7.5
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1536225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.759670629260352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.75291980570991	12.568797478598029
2	6.333950413915597	1.996419446107542
3	2.253850319019611	1.0655981602620732
4	1.1957800727904606	0.7538040036884252
5	0.7393794622811753	0.5826188397795476
6	0.5168391677126738	0.48871290308716747
7	0.40245688106819216	0.44398115216798806
8	0.3362416107106316	0.4239245629321227
9	0.29508124890063236	0.41853449623902833
>10	4.465275954226317	17.865871392873462
>50	2.05015668868254	23.3058609558056
>100	1.6505921147744602	38.354789055981065
>500	0.005399521261214893	0.5085388790441452
>1k	0.0016613911572968898	0.6933906996672197
>5k	4.1534778932422246E-4	0.5291579737666199
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8084	0.5262249995931586	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3909	0.2544549138309818	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3389	0.22060570554443523	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1806	0.11756090416442905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024019918957184007	0.0
2	0.0	0.0	0.0	0.08885417175218474	0.0
3	0.0	0.0	0.0	0.12231281225080962	0.0
4	0.0	0.0	0.0	0.17536493677683934	0.0
5	0.0	0.0	0.0	0.268124786408241	0.0
6	0.0	0.0	0.0	0.342267571482042	0.0
7	0.0	0.0	0.0	0.38737815098699735	0.0
8	0.0	0.0	0.0	0.4936125893016973	0.0
9	0.0	0.0	0.0	0.5335155983010301	0.0
10	6.509463132028186E-5	0.0	0.0	0.6139074679815782	0.0
11	6.509463132028186E-5	0.0	0.0	0.7258051392211428	0.0
12	6.509463132028186E-5	0.0	0.0	0.8009894383960683	0.0
13	6.509463132028186E-5	0.0	0.0	0.8308678741720776	0.0
14	6.509463132028186E-5	0.0	0.0	0.8442122735927354	0.0
15	6.509463132028186E-5	0.0	0.0	0.8681020032872789	0.0
16	1.301892626405637E-4	0.0	0.0	0.9222607365457534	0.0
17	1.301892626405637E-4	0.0	0.0	0.9887223551237612	0.0
18	1.301892626405637E-4	0.0	0.0	1.0624094777783202	0.0
19	1.301892626405637E-4	0.0	0.0	1.10393985256066	6.509463132028186E-5
20	1.301892626405637E-4	0.0	0.0	1.1442334293479146	6.509463132028186E-5
21	1.301892626405637E-4	0.0	0.0	1.206268612996143	6.509463132028186E-5
22	1.301892626405637E-4	0.0	0.0	1.2720141906296278	6.509463132028186E-5
23	1.301892626405637E-4	0.0	0.0	1.3453758401275855	6.509463132028186E-5
24	1.301892626405637E-4	0.0	0.0	1.400901560643786	6.509463132028186E-5
25	1.301892626405637E-4	0.0	0.0	1.4456215723608195	6.509463132028186E-5
26	1.301892626405637E-4	0.0	0.0	1.4931406532246252	6.509463132028186E-5
27	1.301892626405637E-4	0.0	0.0	1.5426125730280396	6.509463132028186E-5
28	1.301892626405637E-4	0.0	0.0	1.5939071425084217	6.509463132028186E-5
29	1.301892626405637E-4	0.0	0.0	1.6571791241517355	6.509463132028186E-5
30	1.301892626405637E-4	0.0	0.0	1.726765285033117	6.509463132028186E-5
31	1.301892626405637E-4	0.0	0.0	1.7893212257319078	6.509463132028186E-5
32	1.301892626405637E-4	0.0	0.0	1.848296961708083	6.509463132028186E-5
33	1.301892626405637E-4	0.0	0.0	1.9106576185129132	6.509463132028186E-5
34	1.301892626405637E-4	0.0	0.0	1.9739296001562272	6.509463132028186E-5
35	1.301892626405637E-4	0.0	0.0	2.0605705544435224	6.509463132028186E-5
36	1.301892626405637E-4	0.0	0.0	2.130221809956224	6.509463132028186E-5
37	1.301892626405637E-4	0.0	0.0	2.2033230809289	6.509463132028186E-5
38	1.301892626405637E-4	0.0	0.0	2.2742762290680076	6.509463132028186E-5
39	1.301892626405637E-4	0.0	0.0	2.3499812852934956	6.509463132028186E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	30	2.1659816E-6	45.000004	27
CTACGGC	30	2.1659816E-6	45.000004	18
ACCGTAT	20	7.0340285E-4	45.000004	18
TCGACAC	30	2.1659816E-6	45.000004	34
GTCGACA	35	1.2122837E-7	45.0	14
CACGTTA	45	3.8562575E-10	45.0	13
TATACGA	25	3.8914208E-5	45.0	44
TGTACGC	25	3.8914208E-5	45.0	25
CGGTCTA	150	0.0	43.500004	31
TATAGCG	130	0.0	43.26923	1
CGACGGT	160	0.0	42.187504	28
TATACGG	120	0.0	41.250004	2
CGTTTTT	5565	0.0	41.19946	1
TCACGAC	175	0.0	41.14286	25
ACGACGG	175	0.0	41.14286	27
CCGTCGG	50	1.0822987E-9	40.5	20
ACGGGTA	235	0.0	40.212765	5
AGGGATG	4060	0.0	40.17857	6
CGAGGGA	645	0.0	40.116276	4
CGTATGG	135	0.0	40.000004	2
>>END_MODULE