##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549067_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2067154
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26726310666743	31.0	31.0	33.0	30.0	34.0
2	31.613834769930058	31.0	31.0	34.0	30.0	34.0
3	31.71506718899511	31.0	31.0	34.0	30.0	34.0
4	35.43994690284323	37.0	35.0	37.0	33.0	37.0
5	35.28392175909487	37.0	35.0	37.0	33.0	37.0
6	35.37261181314987	37.0	35.0	37.0	33.0	37.0
7	35.81586761315316	37.0	35.0	37.0	35.0	37.0
8	35.83599238373145	37.0	35.0	37.0	35.0	37.0
9	37.47915491540543	39.0	37.0	39.0	35.0	39.0
10	36.83625264494082	39.0	37.0	39.0	32.0	39.0
11	36.488450787894855	39.0	35.0	39.0	32.0	39.0
12	35.832460474642915	37.0	35.0	39.0	31.0	39.0
13	35.53056376061	37.0	35.0	39.0	30.0	39.0
14	36.539355074658204	38.0	35.0	41.0	31.0	41.0
15	36.733673930437696	38.0	35.0	41.0	31.0	41.0
16	36.87888807510229	38.0	35.0	41.0	32.0	41.0
17	36.80319221499705	38.0	35.0	41.0	31.0	41.0
18	36.7354038450933	38.0	35.0	40.0	31.0	41.0
19	36.69116476082576	38.0	35.0	40.0	31.0	41.0
20	36.569704530963826	38.0	35.0	40.0	31.0	41.0
21	36.392875905713844	38.0	35.0	40.0	31.0	41.0
22	36.31854714259315	38.0	35.0	40.0	30.0	41.0
23	36.24969257249339	38.0	35.0	40.0	30.0	41.0
24	36.18152058337211	38.0	35.0	40.0	30.0	41.0
25	36.08193825907504	38.0	35.0	40.0	30.0	41.0
26	35.98635805556819	38.0	34.0	40.0	30.0	41.0
27	35.94104116093914	38.0	34.0	40.0	30.0	41.0
28	35.914692857909955	37.0	35.0	40.0	30.0	41.0
29	35.87585153307398	37.0	35.0	40.0	30.0	41.0
30	35.78650260212834	37.0	34.0	40.0	30.0	41.0
31	35.64337683597835	37.0	34.0	40.0	29.0	41.0
32	35.49596498374093	37.0	34.0	40.0	29.0	41.0
33	35.34810759140345	37.0	34.0	40.0	28.0	41.0
34	35.18649408800699	37.0	34.0	40.0	27.0	41.0
35	35.05218769380511	37.0	34.0	40.0	27.0	41.0
36	34.9087605471097	37.0	34.0	40.0	26.0	41.0
37	34.85902598451784	37.0	34.0	40.0	26.0	41.0
38	34.81931970235406	37.0	33.0	40.0	26.0	41.0
39	34.746239032021805	37.0	33.0	40.0	25.0	41.0
40	34.57040839724568	36.0	33.0	40.0	24.0	41.0
41	34.511483904924354	36.0	33.0	40.0	24.0	41.0
42	34.450806761373364	36.0	33.0	40.0	24.0	41.0
43	34.37887114361097	36.0	33.0	40.0	24.0	41.0
44	34.32279888194107	36.0	33.0	40.0	24.0	41.0
45	34.272881942999895	36.0	33.0	40.0	24.0	41.0
46	34.2417555731213	35.0	33.0	40.0	24.0	41.0
47	34.17981437280435	35.0	33.0	40.0	24.0	41.0
48	34.10475658804327	35.0	33.0	40.0	23.0	41.0
49	34.028612285296596	36.0	33.0	40.0	24.0	41.0
50	33.901775097549574	35.0	33.0	39.0	24.0	41.0
51	33.749763684756914	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	10.0
13	16.0
14	31.0
15	72.0
16	215.0
17	563.0
18	1184.0
19	2256.0
20	3927.0
21	6035.0
22	9029.0
23	13037.0
24	18325.0
25	24955.0
26	33009.0
27	39329.0
28	44287.0
29	51032.0
30	60664.0
31	73489.0
32	90503.0
33	114726.0
34	187075.0
35	268343.0
36	152652.0
37	187638.0
38	262845.0
39	421676.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.22197475369518	23.339964027837308	27.005583522079146	13.432477696388368
2	32.40909966069292	24.79921670083603	28.42047568782974	14.371207950641315
3	30.204329237202455	25.189898769032204	30.65117548087854	13.9545965128868
4	27.29588603461571	27.573610867888892	29.3582384282932	15.7722646692022
5	23.046275217037532	32.62301695954922	29.22341538172773	15.107292441685525
6	22.276279367671687	41.878205494123804	25.752072656415535	10.093442481788973
7	86.2691894266223	4.5175153858880375	6.963438621408952	2.249856566080708
8	86.46327269279406	4.205008431882675	6.939492655119067	2.3922262202042037
9	81.56242834350996	6.111929735278552	8.684742404291116	3.6408995169203653
10	46.12239823448084	25.65769168625076	15.069898033721726	13.15001204554668
11	39.70942658360238	23.493701968987313	21.44920020472592	15.347671242684385
12	36.88394768846443	22.009293937461845	25.798997075205815	15.307761298867911
13	23.28375147666792	34.12546912324868	25.548604506485727	17.042174893597668
14	17.733173242051635	37.02326967415103	27.69246993692778	17.55108714686956
15	17.081649456208876	24.644946627101803	41.88188204652386	16.391521870165455
16	19.092675243353906	20.214217228131044	41.220247741580934	19.472859786934112
17	18.666582170462384	20.834490318573266	29.667794465240615	30.831133045723735
18	21.76127177752601	23.747625963039038	33.50132597764849	20.989776281786458
19	28.564199861258523	24.533730916999893	26.150930216132906	20.751139005608678
20	29.727683568810065	23.152217976986716	26.735115042227136	20.38498341197608
21	23.973927438400818	26.158089818175135	28.613833318659378	21.254149424764677
22	23.48068890851867	24.63870616315959	25.457803337342067	26.422801590979677
23	21.439089685625746	28.805642927425822	26.117792868842866	23.63747451810557
24	20.90923075881139	23.883174644946628	35.89093991062108	19.316654685620907
25	20.40660734517119	24.58157447389019	32.655041665981344	22.35677651495728
26	19.27921190196763	31.549125028904474	27.30435178027375	21.867311288854143
27	19.65155958385297	32.07071171281869	27.658365075848245	20.6193636274801
28	17.815411914158307	28.933886880222758	33.756362612558135	19.494338593060796
29	18.691785904678607	25.477298740200293	33.45551419971613	22.375401155404965
30	20.853453588847277	29.517829827869623	29.395294206430677	20.233422376852424
31	26.927892164783078	26.614707951125073	25.891781647617933	20.565618236473917
32	28.10158314281374	25.07728016393554	27.56156532120974	19.25957137204098
33	26.773089958464634	26.925570131688303	25.529060727938024	20.772279181909038
34	21.135483858483692	26.95116087142032	28.284056243511614	23.629299026584377
35	21.579814566307107	25.899086376728587	29.64167159292438	22.87942746403993
36	28.25125752604789	26.68301442466309	25.43322848708901	19.63249956220001
37	21.88796770825976	31.728066704270702	27.390218629091013	18.993746958378523
38	22.052541803852062	31.53117764810943	24.192150173620348	22.22413037441816
39	22.24865684898174	29.932602989424105	26.571556836113807	21.247183325480346
40	24.33529383877544	25.979728651082603	26.900995281435247	22.783982228706716
41	18.961577124877973	25.688410249067072	28.64493888699149	26.70507373906347
42	22.028499086183224	26.354785371578508	25.44522565807869	26.171489884159577
43	21.596939560381085	26.469193877185738	27.27019854350474	24.66366801892844
44	20.450290592766674	28.346412507244263	28.55466985043204	22.648627049557025
45	19.536328691524677	33.46243192331099	24.17681508005693	22.824424305107406
46	21.4384124259731	32.11299206541941	25.553587202501603	20.895008306105883
47	22.13923103939039	28.13225332994059	26.890449381129805	22.83806624953922
48	22.434322745184925	25.790434578168824	29.89191903457604	21.88332364207021
49	21.42564124395183	26.251358147481994	30.99154683202122	21.33145377654495
50	20.464464669782707	31.461129649750337	26.776089251212053	21.298316429254907
51	19.76664534911284	30.967891119868185	25.60220477042349	23.663258760595486
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1491.0
1	2955.5
2	4420.0
3	9245.5
4	14071.0
5	10671.0
6	7271.0
7	7541.0
8	7811.0
9	8153.0
10	8495.0
11	8660.5
12	8826.0
13	8649.0
14	8472.0
15	8484.0
16	8496.0
17	8526.0
18	8556.0
19	7954.5
20	7353.0
21	7691.5
22	8030.0
23	8923.0
24	9816.0
25	10925.5
26	15082.5
27	18130.0
28	22694.0
29	27258.0
30	33727.0
31	40196.0
32	41782.5
33	43369.0
34	51157.0
35	58945.0
36	60878.5
37	62812.0
38	72646.0
39	82480.0
40	104060.0
41	125640.0
42	147389.0
43	169138.0
44	172877.5
45	176617.0
46	175534.0
47	174451.0
48	167418.0
49	160385.0
50	153463.5
51	146542.0
52	132798.5
53	119055.0
54	111706.0
55	104357.0
56	97635.0
57	90913.0
58	88355.0
59	85797.0
60	81490.0
61	77183.0
62	69167.0
63	61151.0
64	50562.5
65	39974.0
66	34252.0
67	28530.0
68	23881.5
69	19233.0
70	17007.0
71	14781.0
72	12406.5
73	10032.0
74	8662.0
75	5293.5
76	3295.0
77	2913.0
78	2531.0
79	1791.0
80	1051.0
81	852.0
82	653.0
83	388.0
84	123.0
85	89.0
86	55.0
87	39.5
88	24.0
89	16.5
90	9.0
91	8.5
92	8.0
93	4.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2067154.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.82299696341885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.92570793518102	13.445899418926524
2	6.777742851626405	2.2804389482348966
3	2.4504018773258904	1.236693100242279
4	1.35236746682271	0.9100349515113957
5	0.8129580896928486	0.683819573714479
6	0.5942036313616642	0.5997771531629839
7	0.4087002641016999	0.4812894311351967
8	0.34162513204208345	0.45977268471772265
9	0.2663435402004004	0.40326269112158053
>10	3.7883797905783467	16.07762848043429
>50	1.7720317064421576	21.44849017889693
>100	1.4988297208670502	40.03735624603017
>500	0.008103346627455769	0.8748988162352577
>1k	0.002315241893558791	0.725236102762404
>5k	2.894052366948489E-4	0.3354022228739394
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6889	0.3332601247899286	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4960	0.23994341979359063	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2703	0.13075948864961198	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03178282798475585	0.0
2	0.0	0.0	0.0	0.09525173257531853	0.0
3	0.0	0.0	0.0	0.13163025106015325	0.0
4	0.0	0.0	0.0	0.21619095626160412	0.0
5	0.0	0.0	0.0	0.37263793602218315	0.0
6	0.0	0.0	0.0	0.5185390154773181	0.0
7	0.0	0.0	0.0	0.6063408918735614	0.0
8	0.0	0.0	0.0	0.8249022569194168	0.0
9	0.0	0.0	0.0	0.8950470066574624	0.0
10	0.0	0.0	0.0	1.0286606609860707	0.0
11	0.0	0.0	0.0	1.1822534750676534	0.0
12	0.0	0.0	0.0	1.3178505326647167	0.0
13	0.0	0.0	0.0	1.3697092717813961	0.0
14	0.0	0.0	0.0	1.3880920337817115	0.0
15	0.0	0.0	0.0	1.418713845219079	0.0
16	0.0	0.0	0.0	1.4915192578782228	0.0
17	0.0	0.0	0.0	1.575160824979658	0.0
18	0.0	0.0	0.0	1.699341219860736	0.0
19	4.8375689474514235E-5	0.0	0.0	1.7582628096406945	0.0
20	4.8375689474514235E-5	0.0	0.0	1.819651559583853	0.0
21	9.675137894902847E-5	0.0	0.0	1.9016967289326292	0.0
22	9.675137894902847E-5	0.0	0.0	2.0010603951132815	0.0
23	9.675137894902847E-5	0.0	0.0	2.1096638179835656	0.0
24	9.675137894902847E-5	0.0	0.0	2.1921443685376127	0.0
25	9.675137894902847E-5	0.0	0.0	2.263837140338843	0.0
26	9.675137894902847E-5	0.0	0.0	2.3345623983505823	0.0
27	9.675137894902847E-5	0.0	0.0	2.4031591260254435	0.0
28	9.675137894902847E-5	0.0	0.0	2.478189820400415	0.0
29	9.675137894902847E-5	0.0	0.0	2.5679751000651136	0.0
30	9.675137894902847E-5	0.0	0.0	2.6710636943353037	0.0
31	9.675137894902847E-5	0.0	0.0	2.7731363991265288	0.0
32	9.675137894902847E-5	0.0	0.0	2.862824927412278	0.0
33	9.675137894902847E-5	0.0	0.0	2.9563835108559884	0.0
34	1.451270684235427E-4	0.0	0.0	3.0553601715208445	0.0
35	1.451270684235427E-4	0.0	0.0	3.177702290201891	0.0
36	1.9350275789805694E-4	0.0	0.0	3.28335479601423	0.0
37	2.4187844737257118E-4	0.0	0.0	3.3896845614792124	0.0
38	2.902541368470854E-4	0.0	0.0	3.498578238486344	0.0
39	2.902541368470854E-4	0.0	0.0	3.611438722030386	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	55	1.8189894E-12	45.000004	1
ATACGAC	20	7.0345955E-4	45.0	22
TATACGA	20	7.0345955E-4	45.0	21
TCACGAC	185	0.0	43.783783	25
CGGTCTA	180	0.0	43.75	31
CGTTAGG	205	0.0	42.80488	2
TTGCGTA	75	0.0	42.0	43
CGAATAT	340	0.0	41.691177	14
CACGACG	195	0.0	41.53846	26
GTGCGAT	65	0.0	41.53846	30
CTACGAA	350	0.0	41.142857	11
TAGGGCG	565	0.0	40.22124	5
ATTACGT	40	3.460009E-7	39.375	37
AGTCGTA	40	3.460009E-7	39.375	20
CGGCTAC	655	0.0	39.160305	25
GCGTTAG	115	0.0	39.130432	1
TAACGGG	575	0.0	38.739132	3
CGCGATT	35	6.2507115E-6	38.571426	23
CCGCTCG	70	0.0	38.571426	19
CAAATCG	35	6.2507115E-6	38.571426	27
>>END_MODULE