FastQCFastQC Report
Sat 18 Jun 2016
SRR3549066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1660238
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68500.41259144773219264No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32210.19400832892633466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT406.8175723E-945.00000417
CTATCGG207.0341927E-445.0000042
ATCGCTA207.0341927E-445.00000411
TAACGCG253.8915576E-545.01
TTGCGTA351.2123564E-745.034
TCGGATG302.1660871E-644.9999961
CGACGGT2250.041.028
ACGGGCT2400.040.3124965
ACGGTCT2300.040.108730
GGGCGAT27400.039.0875937
AACGTAG750.039.0000041
TGCGTAG750.039.0000041
CGTTGAT3150.038.57142625
TCGACAA2650.038.20754619
ATTAGCG1300.038.076921
ATATACG659.094947E-1238.076921
GTTCACG659.094947E-1238.076921
GGTACCT11650.038.047218
CGTTTTT51200.038.01271
GTAGGGT5700.037.8947374
Produced by FastQC (version 0.11.5)