##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549066_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1660238
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.353410173722082	31.0	31.0	33.0	30.0	34.0
2	31.706600499446466	31.0	31.0	34.0	30.0	34.0
3	31.823438567241563	31.0	31.0	34.0	30.0	34.0
4	35.54600605455362	37.0	35.0	37.0	33.0	37.0
5	35.41433396898517	37.0	35.0	37.0	33.0	37.0
6	35.4898725363472	37.0	35.0	37.0	33.0	37.0
7	35.85985503283264	37.0	35.0	37.0	35.0	37.0
8	35.87416322238137	37.0	35.0	37.0	35.0	37.0
9	37.554469298980024	39.0	37.0	39.0	35.0	39.0
10	36.95958591479053	39.0	37.0	39.0	32.0	39.0
11	36.6339844046456	39.0	35.0	39.0	32.0	39.0
12	36.042269843239346	37.0	35.0	39.0	32.0	39.0
13	35.812384128058746	37.0	35.0	39.0	31.0	39.0
14	36.84718094634625	39.0	35.0	41.0	31.0	41.0
15	37.00081494339968	39.0	35.0	41.0	32.0	41.0
16	37.096362087845236	39.0	35.0	41.0	32.0	41.0
17	37.01051415519944	38.0	35.0	41.0	32.0	41.0
18	36.94703952083978	38.0	35.0	41.0	32.0	41.0
19	36.90344697567457	38.0	35.0	41.0	31.0	41.0
20	36.77398662119528	38.0	35.0	41.0	31.0	41.0
21	36.59448344153067	38.0	35.0	40.0	31.0	41.0
22	36.529334348448835	38.0	35.0	40.0	31.0	41.0
23	36.44093678135304	38.0	35.0	40.0	31.0	41.0
24	36.37484926859884	38.0	35.0	40.0	31.0	41.0
25	36.25731250579736	38.0	35.0	40.0	30.0	41.0
26	36.18313940531418	38.0	35.0	40.0	30.0	41.0
27	36.15301721801332	38.0	35.0	40.0	30.0	41.0
28	36.12247280209223	38.0	35.0	40.0	30.0	41.0
29	36.063799888931584	38.0	35.0	40.0	30.0	41.0
30	35.9525001837086	38.0	35.0	40.0	30.0	41.0
31	35.80994351412267	38.0	34.0	40.0	30.0	41.0
32	35.66303566115219	38.0	34.0	40.0	29.0	41.0
33	35.495262727392095	38.0	34.0	40.0	29.0	41.0
34	35.324341449840325	37.0	34.0	40.0	27.0	41.0
35	35.18426032894079	37.0	34.0	40.0	27.0	41.0
36	35.02501448587492	37.0	34.0	40.0	26.0	41.0
37	34.965703712359314	37.0	34.0	40.0	26.0	41.0
38	34.92212682759941	37.0	34.0	40.0	26.0	41.0
39	34.82897150890415	37.0	34.0	40.0	26.0	41.0
40	34.65461036309252	36.0	33.0	40.0	25.0	41.0
41	34.60055425788351	36.0	33.0	40.0	25.0	41.0
42	34.4991338591214	36.0	33.0	40.0	25.0	41.0
43	34.41206260789116	36.0	33.0	40.0	24.0	41.0
44	34.34314056177488	36.0	33.0	40.0	24.0	41.0
45	34.29409699091335	36.0	33.0	40.0	24.0	41.0
46	34.2595507391109	35.0	33.0	40.0	24.0	41.0
47	34.17655480720234	35.0	33.0	40.0	24.0	41.0
48	34.09352394054346	35.0	33.0	40.0	23.0	41.0
49	34.00936612702516	35.0	33.0	39.0	24.0	41.0
50	33.90146653672546	35.0	33.0	39.0	24.0	41.0
51	33.728054652405255	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	7.0
14	25.0
15	54.0
16	135.0
17	391.0
18	850.0
19	1665.0
20	2948.0
21	4623.0
22	6809.0
23	9643.0
24	13683.0
25	18993.0
26	25148.0
27	30228.0
28	33901.0
29	38738.0
30	46475.0
31	56655.0
32	69743.0
33	89737.0
34	146547.0
35	209146.0
36	128867.0
37	162009.0
38	219008.0
39	344022.0
40	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.2310102527469	22.568270332325845	27.59580252951685	13.604916885410406
2	32.63598351561644	25.58645206289701	28.25203374455951	13.525530676927042
3	30.373657270825028	25.363833378106033	30.080626994442966	14.181882356625977
4	27.79962872792937	27.256995683751366	29.3973514640672	15.546024124252064
5	23.949036222517496	32.43812031768939	29.01240665494947	14.600436804843643
6	22.055632987559616	41.96404370939588	25.896166694172763	10.08415660887174
7	86.74575572899789	4.405151550560824	6.768909035933403	2.0801836845078836
8	87.09269393906175	3.928653602676243	7.012307873931328	1.9663445843306804
9	82.64339209197718	5.721890475943811	8.425659453644597	3.2090579784344175
10	46.00792175579646	26.55215697990288	15.09789560291958	12.34202566138108
11	38.89635100509686	23.18938610006517	22.08020777743914	15.834055117398831
12	35.974179605574626	21.2275589403447	26.501501591940436	16.29675986214025
13	24.116602559392085	31.52560054642768	25.80322821185878	18.554568682321452
14	18.940778370330037	34.446747996371606	28.418094273230704	18.194379360067654
15	18.855248464376796	23.889165288350224	39.40519371319052	17.850392534082463
16	20.442310078434538	20.341059534837775	38.76570708536969	20.450923301358
17	19.912626984805794	20.147292135223985	30.072555862472726	29.867525017497492
18	23.04549106814806	23.05488731133729	31.867539473256244	22.032082147258407
19	28.66034869699405	24.15442846146155	25.577236516692185	21.60798632485222
20	30.96122363179255	21.83403825234695	26.38362692577811	20.821111190082387
21	24.56954966697546	25.514534663102516	28.257755815732445	21.65815985418958
22	24.780603744764303	22.977308072698012	26.324599244204745	25.917488938332937
23	22.431783876769476	27.4313080413772	26.12860324844992	24.008304833403404
24	21.791694925667286	23.497534690809392	35.12412075859003	19.58664962493329
25	21.476740081843687	24.50196899480677	31.886572888947246	22.134718034402294
26	20.40641161086543	29.177081840073534	27.39251842205756	23.023988127003477
27	20.93121588591515	30.268612090555692	27.44684798203631	21.353324041492847
28	19.473653777349995	27.816915406104425	31.98722110926265	20.722209707282932
29	20.545728985844196	24.31344180774082	32.656161345542024	22.48466786087296
30	21.713453131418508	27.812879840119308	30.062918689970957	20.410748338491228
31	27.083707275703844	26.941318051990137	25.112785034434822	20.862189637871197
32	28.86098258201535	24.733682761146294	26.628170178010624	19.777164478827736
33	27.717351367695475	26.378567410214682	25.067008465051394	20.83707275703845
34	21.698756443353304	27.127375713602508	28.02586135240851	23.14800649063568
35	23.03151716802049	25.344318103789938	29.184490416434272	22.439674311755304
36	28.102235944485066	27.049736242635092	24.96262584039156	19.885401972488282
37	23.60245940642245	30.905870122235484	26.720446104715105	18.771224366626953
38	22.71078002069583	30.79534380010577	24.417342573775567	22.07653360542284
39	22.96206929367958	29.11666881495304	26.24310490423662	21.678156987130762
40	24.97918973062898	25.74679052039527	26.6072695601474	22.66675018882835
41	20.893630913158233	25.057190595565217	28.185115628000325	25.864062863276228
42	22.4797288099658	26.398323613843317	24.416258391869118	26.70568918432177
43	22.57375147418623	26.13016928898146	26.645396623857543	24.650682612974766
44	21.692371816570876	27.630616815179508	27.61628152108312	23.060729847166492
45	20.038693247594622	32.2354987658396	25.40533345219179	22.32047453437399
46	22.224946061950153	30.393353242125524	25.718481326171304	21.663219369753012
47	22.81492171604312	27.91569642424761	26.69563038552304	22.57375147418623
48	23.347736890734943	25.539952705575946	29.314050154254996	21.79826024943412
49	22.574173100483183	25.41768107945969	29.49968618957041	22.508459630486712
50	21.575882493955685	30.06303915462723	27.18104271797176	21.18003563344533
51	20.572833533505438	29.445296397263526	26.366280015274917	23.61559005395612
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1332.0
1	2805.0
2	4278.0
3	9113.5
4	13949.0
5	10471.0
6	6993.0
7	6875.5
8	6758.0
9	6920.5
10	7083.0
11	7146.5
12	7210.0
13	7217.5
14	7225.0
15	7245.5
16	7266.0
17	6998.5
18	6731.0
19	6264.0
20	5797.0
21	5837.5
22	5878.0
23	6842.5
24	7807.0
25	8948.0
26	11964.5
27	13840.0
28	16756.5
29	19673.0
30	22656.5
31	25640.0
32	27996.5
33	30353.0
34	35078.5
35	39804.0
36	41961.0
37	44118.0
38	54321.0
39	64524.0
40	76121.5
41	87719.0
42	106088.0
43	124457.0
44	129469.0
45	134481.0
46	134220.0
47	133959.0
48	125643.5
49	117328.0
50	114578.0
51	111828.0
52	103219.0
53	94610.0
54	90094.0
55	85578.0
56	83774.0
57	81970.0
58	79562.0
59	77154.0
60	74355.5
61	71557.0
62	65326.0
63	59095.0
64	51217.5
65	43340.0
66	37038.0
67	30736.0
68	26740.5
69	22745.0
70	19893.5
71	17042.0
72	14816.0
73	12590.0
74	9959.0
75	6046.0
76	4764.0
77	3841.0
78	2918.0
79	2163.0
80	1408.0
81	1154.0
82	900.0
83	553.5
84	207.0
85	141.5
86	76.0
87	72.5
88	69.0
89	45.0
90	21.0
91	14.0
92	7.0
93	4.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1660238.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.394649432649555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.47501140774288	13.650453126608623
2	6.199347812819976	2.1567096383013253
3	2.184671986416337	1.1400480998712694
4	1.2419652323400598	0.86414199296378
5	0.753595260048063	0.6554262681321217
6	0.6025824439908343	0.6289026220493851
7	0.5065806482319737	0.6168254949751683
8	0.38451051217320686	0.535074044993717
9	0.3767038839877507	0.5897368801695971
>10	6.171362620041088	27.186385856923785
>50	2.03866700373207	24.69155848660899
>100	1.0576803947087914	25.825026984767874
>500	0.005926356858947973	0.675158555477941
>1k	0.0010458276809908187	0.3691723806134098
>5k	3.486092269969396E-4	0.415379567543012
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6850	0.41259144773219264	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3221	0.19400832892633466	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023550840301209828	0.0
2	0.0	0.0	0.0	0.062039297980169106	0.0
3	0.0	0.0	0.0	0.08498781500001806	0.0
4	6.0232328136086515E-5	0.0	0.0	0.11974186833454	0.0
5	6.0232328136086515E-5	0.0	0.0	0.19671878369245854	0.0
6	6.0232328136086515E-5	0.0	0.0	0.2746594163005545	0.0
7	6.0232328136086515E-5	0.0	0.0	0.32170086457483804	0.0
8	6.0232328136086515E-5	0.0	0.0	0.42487884267195425	0.0
9	6.0232328136086515E-5	0.0	0.0	0.46366846199159395	0.0
10	6.0232328136086515E-5	0.0	0.0	0.5394407307867908	0.0
11	6.0232328136086515E-5	0.0	0.0	0.6558095887457099	0.0
12	6.0232328136086515E-5	0.0	0.0	0.747784353809514	0.0
13	6.0232328136086515E-5	0.0	0.0	0.7870558317542424	0.0
14	6.0232328136086515E-5	0.0	0.0	0.8045834392418436	0.0
15	6.0232328136086515E-5	0.0	0.0	0.8324107748407156	0.0
16	6.0232328136086515E-5	0.0	0.0	0.8914986887422165	0.0
17	6.0232328136086515E-5	0.0	0.0	0.9681744424594546	0.0
18	6.0232328136086515E-5	0.0	0.0	1.0590650256168093	0.0
19	6.0232328136086515E-5	0.0	0.0	1.1071906557975424	0.0
20	6.0232328136086515E-5	0.0	0.0	1.1583279023850797	0.0
21	6.0232328136086515E-5	0.0	0.0	1.2254869482568163	0.0
22	1.2046465627217303E-4	0.0	0.0	1.302704792927279	0.0
23	1.2046465627217303E-4	0.0	0.0	1.391005385974782	0.0
24	1.2046465627217303E-4	0.0	0.0	1.4576223408932936	0.0
25	1.2046465627217303E-4	0.0	0.0	1.5108677189655941	0.0
26	1.2046465627217303E-4	0.0	0.0	1.5676668043979236	0.0
27	1.2046465627217303E-4	0.0	0.0	1.6274172739089214	0.0
28	1.2046465627217303E-4	0.0	0.0	1.6900588951704514	0.0
29	1.2046465627217303E-4	0.0	0.0	1.7605909514178089	0.0
30	1.2046465627217303E-4	0.0	0.0	1.838652048682177	0.0
31	1.8069698440825954E-4	0.0	0.0	1.9153880347275511	0.0
32	1.8069698440825954E-4	0.0	0.0	1.9944730815702327	0.0
33	1.8069698440825954E-4	0.0	0.0	2.0738592900535946	0.0
34	1.8069698440825954E-4	0.0	0.0	2.156317347271897	0.0
35	1.8069698440825954E-4	0.0	0.0	2.2573871938842505	0.0
36	2.4092931254434606E-4	0.0	0.0	2.349000564979238	0.0
37	2.4092931254434606E-4	0.0	0.0	2.4428425322152605	0.0
38	2.4092931254434606E-4	0.0	0.0	2.5400575098269043	0.0
39	2.4092931254434606E-4	0.0	0.0	2.639139689610767	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGT	40	6.8175723E-9	45.000004	17
CTATCGG	20	7.0341927E-4	45.000004	2
ATCGCTA	20	7.0341927E-4	45.000004	11
TAACGCG	25	3.8915576E-5	45.0	1
TTGCGTA	35	1.2123564E-7	45.0	34
TCGGATG	30	2.1660871E-6	44.999996	1
CGACGGT	225	0.0	41.0	28
ACGGGCT	240	0.0	40.312496	5
ACGGTCT	230	0.0	40.1087	30
GGGCGAT	2740	0.0	39.087593	7
AACGTAG	75	0.0	39.000004	1
TGCGTAG	75	0.0	39.000004	1
CGTTGAT	315	0.0	38.571426	25
TCGACAA	265	0.0	38.207546	19
ATTAGCG	130	0.0	38.07692	1
ATATACG	65	9.094947E-12	38.07692	1
GTTCACG	65	9.094947E-12	38.07692	1
GGTACCT	1165	0.0	38.04721	8
CGTTTTT	5120	0.0	38.0127	1
GTAGGGT	570	0.0	37.894737	4
>>END_MODULE