##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2305166
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.285846659199382	31.0	31.0	33.0	30.0	34.0
2	31.648673457790025	31.0	31.0	34.0	30.0	34.0
3	31.75414178414917	31.0	31.0	34.0	30.0	34.0
4	35.465624601438684	37.0	35.0	37.0	33.0	37.0
5	35.31466627566084	37.0	35.0	37.0	33.0	37.0
6	35.39124991432287	37.0	35.0	37.0	33.0	37.0
7	35.81282302445898	37.0	35.0	37.0	35.0	37.0
8	35.82425387152162	37.0	35.0	37.0	35.0	37.0
9	37.47482393892674	39.0	37.0	39.0	35.0	39.0
10	36.8332289301508	39.0	37.0	39.0	32.0	39.0
11	36.49249858795419	39.0	35.0	39.0	32.0	39.0
12	35.93253154002792	37.0	35.0	39.0	31.0	39.0
13	35.643384033948095	37.0	35.0	39.0	30.0	39.0
14	36.692180519754324	38.0	35.0	41.0	31.0	41.0
15	36.847553278158706	38.0	35.0	41.0	31.0	41.0
16	36.95883853917679	38.0	35.0	41.0	32.0	41.0
17	36.89004349361391	38.0	35.0	41.0	32.0	41.0
18	36.8033725120013	38.0	35.0	40.0	31.0	41.0
19	36.75926332420312	38.0	35.0	40.0	31.0	41.0
20	36.622772503151616	38.0	35.0	40.0	31.0	41.0
21	36.44353074789408	38.0	35.0	40.0	31.0	41.0
22	36.37085094956285	38.0	35.0	40.0	30.0	41.0
23	36.32134995917865	38.0	35.0	40.0	30.0	41.0
24	36.237428020368164	38.0	35.0	40.0	30.0	41.0
25	36.10364155987031	38.0	35.0	40.0	30.0	41.0
26	36.00117562032409	38.0	35.0	40.0	30.0	41.0
27	35.965254563011946	38.0	34.0	40.0	30.0	41.0
28	35.902289900163375	38.0	35.0	40.0	30.0	41.0
29	35.86656709321585	38.0	35.0	40.0	30.0	41.0
30	35.78344943487801	38.0	34.0	40.0	29.0	41.0
31	35.6322381988976	37.0	34.0	40.0	29.0	41.0
32	35.475729730527	37.0	34.0	40.0	29.0	41.0
33	35.31836622611994	37.0	34.0	40.0	27.0	41.0
34	35.12785196380651	37.0	34.0	40.0	27.0	41.0
35	34.9727794007026	37.0	34.0	40.0	26.0	41.0
36	34.8192720177202	37.0	34.0	40.0	25.0	41.0
37	34.76490760318346	37.0	33.0	40.0	25.0	41.0
38	34.72249590701928	37.0	33.0	40.0	25.0	41.0
39	34.64499649916752	37.0	33.0	40.0	24.0	41.0
40	34.4999436049291	37.0	33.0	40.0	24.0	41.0
41	34.405510926328084	36.0	33.0	40.0	24.0	41.0
42	34.31806950128537	36.0	33.0	40.0	23.0	41.0
43	34.244063984979825	36.0	33.0	40.0	23.0	41.0
44	34.184969325419516	36.0	33.0	40.0	23.0	41.0
45	34.14769218355641	36.0	33.0	40.0	23.0	41.0
46	34.09710840781098	36.0	33.0	40.0	23.0	41.0
47	34.039525136150715	35.0	33.0	40.0	23.0	41.0
48	33.96677072280261	35.0	33.0	40.0	23.0	41.0
49	33.88783757872535	36.0	33.0	40.0	24.0	41.0
50	33.77548471563436	35.0	33.0	39.0	23.0	41.0
51	33.6373636432257	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	4.0
13	12.0
14	34.0
15	86.0
16	269.0
17	719.0
18	1515.0
19	2879.0
20	4873.0
21	7634.0
22	11172.0
23	15599.0
24	22091.0
25	29921.0
26	38935.0
27	45907.0
28	51041.0
29	57719.0
30	67227.0
31	80599.0
32	97984.0
33	124257.0
34	200152.0
35	279696.0
36	170797.0
37	212542.0
38	297223.0
39	483990.0
40	284.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.610434996872243	24.335904659360757	27.9765969131941	16.077063430572895
2	31.976308864524288	25.82594919411444	28.388497834863085	13.809244106498186
3	29.731958566107604	25.134155197499876	30.749152121799472	14.384734114593048
4	27.478368152228516	27.198344934811637	29.977407266982077	15.345879645977773
5	23.402696378482073	32.590364424948135	28.706349130604913	15.300590065964881
6	22.369495298820127	40.9474198387448	26.624243113077323	10.058841749357747
7	85.03686936211969	4.646693556993292	7.958776070790563	2.357661010096453
8	85.36417767744275	4.221171056661429	8.088875161268213	2.325776104627606
9	80.63371574975513	6.041387041106801	9.565992210539283	3.758904998598799
10	44.966262733356295	25.4089727160647	16.616026785055826	13.008737765523176
11	37.60349580030245	23.80119262560701	23.24760993351455	15.347701640575991
12	36.74173573616824	20.727574500057695	27.164377749801965	15.366312013972095
13	23.47440488016915	31.525104916522277	26.849519730900074	18.150970472408495
14	18.355944864708228	34.74712884017897	28.25354009212352	18.643386202989287
15	17.668705854589213	24.479712090148823	41.42816612773223	16.42341592752973
16	19.31891239069117	20.19312275124655	40.991668278987284	19.496296579074997
17	18.67757029211779	20.484251459547817	30.495938253470683	30.34223999486371
18	21.465655835631793	24.085206878810464	33.18095095971396	21.26818632584378
19	28.542152712646292	25.01962982275463	26.84821830618706	19.58999915841202
20	30.480407918562047	22.63312056485303	26.99150516708992	19.894966349495004
21	24.199471968613107	25.711033391955283	28.238270042157488	21.851224597274122
22	22.953965137434786	24.48821473160718	26.725667479044894	25.832152651913137
23	22.391662899765137	28.001497506036443	26.95168157087169	22.655158023326734
24	21.804807115843282	23.783840296100152	35.05448197656915	19.356870611487416
25	20.965908745834355	24.81344076738942	31.872368410778222	22.348282075997997
26	20.208045754622443	31.06231828857445	27.375035030015194	21.35460092678792
27	20.839497025376915	30.30224287535041	28.250373291988517	20.607886807284164
28	19.13723350075439	27.78133982541821	33.822206296639806	19.259220377187585
29	19.529396147609326	26.006066374395598	33.43724486653022	21.02729261146486
30	21.112882976757422	27.933432993545804	30.170018124508168	20.783665905188606
31	26.180283762644425	27.160516856486694	25.354746686355778	21.304452694513106
32	27.21773616303555	26.92751845203339	26.579170437183265	19.2755749477478
33	25.92941245879906	27.608554004353696	26.187745264332374	20.274288272514866
34	21.223981266425067	27.45190584973056	28.408366252148436	22.91574663169594
35	22.054333614151865	25.298481757929796	29.77902675989495	22.86815786802339
36	26.666973224487954	27.754790761272723	25.538421094185843	20.03981492005348
37	22.093116070599688	31.353707281818316	27.787803568159518	18.765373079422478
38	21.72919434001716	30.692973955021024	25.715067808565628	21.86276389639618
39	22.249937748517894	29.052007534381474	26.89506959585557	21.802985121245065
40	23.89250925963683	26.668621695791106	28.20803360799179	21.23083543658027
41	19.375480984883517	26.1471841941101	28.806428691035702	25.67090612997068
42	22.57702915972212	27.05865868228145	25.341385392635495	25.022926765360932
43	22.70409159253607	27.323108183965928	26.346996268381538	23.625803955116464
44	21.00785800241718	29.616478813239482	27.76012660259608	21.615536581747257
45	20.551882163800787	32.354763171068804	24.67440522721574	22.418949437914666
46	21.09713573772995	32.02624019268027	25.7889019706173	21.08772209897248
47	22.450183631027006	28.46276580515243	27.181079366952314	21.90597119686825
48	23.439483317036604	25.03489987272066	29.741719251455212	21.783897558787523
49	20.763667345431955	26.623765924015885	29.861016516814843	22.751550213737318
50	19.750638348821735	30.761819322339473	28.269330711974757	21.218211616864036
51	20.085755212422878	31.06999669438123	26.44672878222219	22.3975193109737
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2318.0
1	4781.0
2	7244.0
3	13867.0
4	20490.0
5	15938.5
6	11387.0
7	11027.5
8	10668.0
9	10788.5
10	10909.0
11	11261.0
12	11613.0
13	11249.0
14	10885.0
15	10842.5
16	10800.0
17	10521.0
18	10242.0
19	10180.0
20	10118.0
21	10734.0
22	11350.0
23	11919.5
24	12489.0
25	13559.0
26	17127.5
27	19626.0
28	24725.5
29	29825.0
30	35031.5
31	40238.0
32	47686.5
33	55135.0
34	63396.0
35	71657.0
36	72870.5
37	74084.0
38	83090.0
39	92096.0
40	112906.5
41	133717.0
42	156600.5
43	179484.0
44	183345.5
45	187207.0
46	186828.5
47	186450.0
48	175993.0
49	165536.0
50	158396.0
51	151256.0
52	137843.0
53	124430.0
54	116359.5
55	108289.0
56	103715.5
57	99142.0
58	99552.5
59	99963.0
60	96590.0
61	93217.0
62	84172.5
63	75128.0
64	62638.5
65	50149.0
66	42308.5
67	34468.0
68	30823.0
69	27178.0
70	23019.5
71	18861.0
72	16768.0
73	14675.0
74	11465.5
75	6606.0
76	4956.0
77	3694.0
78	2432.0
79	1879.0
80	1326.0
81	934.0
82	542.0
83	456.5
84	371.0
85	313.5
86	256.0
87	147.5
88	39.0
89	30.0
90	21.0
91	14.0
92	7.0
93	7.0
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2305166.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.125961246825977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.77390029743005	13.83330686248772
2	6.337747620334304	2.170800402760177
3	2.2446539917904693	1.1532557182781045
4	1.2555593424680969	0.8601064256879574
5	0.8197233516251977	0.701927517652572
6	0.5887504972336739	0.6049750919804053
7	0.430211070652773	0.5157444686768433
8	0.341466633340195	0.4678355463734652
9	0.26253215030743426	0.404650388898991
>10	3.7269627425111334	16.285643763415383
>50	1.7432236252349302	21.487091847296306
>100	1.4632870583117095	39.11896821438705
>500	0.008922482055319364	0.9921917192487855
>1k	0.0025492805872341043	0.7337311464267837
>5k	5.098561174468209E-4	0.6697708864294472
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9866	0.4279952072865902	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5475	0.23751001012508427	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3401	0.14753818163203866	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2571	0.11153209790531354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008155594868222071	0.0
2	0.0	0.0	0.0	0.034097327480971	0.0
3	0.0	0.0	0.0	0.05548407359817037	0.0
4	0.0	0.0	0.0	0.10476468939763992	0.0
5	0.0	0.0	0.0	0.1963849891938368	0.0
6	0.0	0.0	0.0	0.30735313639017753	0.0
7	0.0	0.0	0.0	0.3742897474628725	0.0
8	0.0	0.0	0.0	0.5328466583317644	0.0
9	0.0	0.0	0.0	0.598742129634048	0.0
10	0.0	0.0	0.0	0.7045045779783321	0.0
11	4.338082376713868E-5	0.0	0.0	0.8312633450259114	0.0
12	4.338082376713868E-5	0.0	0.0	0.9397587852675252	0.0
13	4.338082376713868E-5	0.0	0.0	0.9827058007969925	0.0
14	4.338082376713868E-5	0.0	0.0	0.9977155658204224	0.0
15	4.338082376713868E-5	0.0	0.0	1.0205772599457046	0.0
16	4.338082376713868E-5	0.0	0.0	1.0745863855357922	0.0
17	4.338082376713868E-5	0.0	0.0	1.1392238129488288	0.0
18	4.338082376713868E-5	0.0	0.0	1.2278942167288602	0.0
19	4.338082376713868E-5	0.0	0.0	1.2711448980246975	0.0
20	8.676164753427736E-5	0.0	0.0	1.321076226180674	0.0
21	8.676164753427736E-5	0.0	0.0	1.3856702727699437	0.0
22	8.676164753427736E-5	0.0	0.0	1.459591196469148	0.0
23	8.676164753427736E-5	0.0	0.0	1.5445742302289727	0.0
24	8.676164753427736E-5	0.0	0.0	1.6077800904576938	0.0
25	8.676164753427736E-5	0.0	0.0	1.6606179338060687	0.0
26	8.676164753427736E-5	0.0	0.0	1.710115453724374	0.0
27	8.676164753427736E-5	0.0	0.0	1.7628231546014475	0.0
28	1.3014247130141603E-4	0.0	0.0	1.8229923571664688	0.0
29	1.3014247130141603E-4	0.0	0.0	1.8879768311696425	0.0
30	1.735232950685547E-4	0.0	0.0	1.9651946974751493	0.0
31	1.735232950685547E-4	0.0	0.0	2.0449720323829172	0.0
32	1.735232950685547E-4	0.0	0.0	2.11845914784445	0.0
33	1.735232950685547E-4	0.0	0.0	2.19411530449434	0.0
34	2.169041188356934E-4	0.0	0.0	2.2702486502056685	0.0
35	3.0366576636997074E-4	0.0	0.0	2.3716296353494717	0.0
36	3.0366576636997074E-4	0.0	0.0	2.4610375131335442	0.0
37	3.470465901371094E-4	0.0	0.0	2.5523541471633715	0.0
38	3.470465901371094E-4	0.0	0.0	2.6366430877429217	0.0
39	3.470465901371094E-4	0.0	0.0	2.729955239666037	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGC	30	2.1664564E-6	45.000004	10
GATATCG	45	3.8562575E-10	45.000004	13
GACGTAT	20	7.0347666E-4	45.0	9
TAACGCG	20	7.0347666E-4	45.0	1
CCGTAGA	20	7.0347666E-4	45.0	22
CCGCGAA	35	1.2126475E-7	45.0	18
ATCGGCT	40	6.8193913E-9	45.0	45
CGTAAGG	275	0.0	44.18182	2
CGGTCTA	225	0.0	44.0	31
GCTACGA	465	0.0	42.09677	10
TATTACG	75	0.0	42.000004	1
TACGGGA	575	0.0	41.47826	4
CATACGA	835	0.0	40.1497	18
CGGTAGT	570	0.0	39.86842	12
TAGGGCG	810	0.0	39.72222	5
TATTTCG	40	3.4602454E-7	39.375	8
CTATGCG	40	3.4602454E-7	39.375	1
TCGCATA	40	3.4602454E-7	39.375	28
TATGCCA	840	0.0	39.375	10
ACGGTCC	40	3.4602454E-7	39.375	41
>>END_MODULE