FastQCFastQC Report
Sat 18 Jun 2016
SRR3549064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1969924
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84120.4270215500699519No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC66690.3385409792458998No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT37330.18949969643498937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA453.8562575E-1045.00000432
ACCGAAA453.8562575E-1045.00000414
CTTGCGA453.8562575E-1045.00000413
GCGATAC1150.045.0000049
ACGTCCA406.8193913E-945.00000426
CGCAACA207.034513E-445.00000436
GCTCGAT207.034513E-445.00000431
TATCGTG207.034513E-445.0000041
CTTACGT800.045.00000422
CGGACGT207.034513E-445.00000423
CGTATGT253.891824E-545.031
CACGGAT253.891824E-545.039
TGTGCGA351.2125201E-745.035
TACGACT253.891824E-545.015
ACGGATT253.891824E-545.045
ACCAGTA551.8189894E-1245.022
AACGAGT253.891824E-545.029
CGTGTCA253.891824E-545.031
ATCGGCA253.891824E-545.011
CGAAGTA351.2125201E-745.018