##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549064_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1969924
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.424988984346605	31.0	31.0	33.0	30.0	34.0
2	31.760959306044295	31.0	31.0	34.0	30.0	34.0
3	31.839716659119844	31.0	31.0	34.0	30.0	34.0
4	35.538173553903604	37.0	35.0	37.0	33.0	37.0
5	35.40675224018795	37.0	35.0	37.0	33.0	37.0
6	35.47675544843354	37.0	35.0	37.0	33.0	37.0
7	35.930521177466744	37.0	35.0	37.0	35.0	37.0
8	35.96909880787279	37.0	35.0	37.0	35.0	37.0
9	37.695981164755594	39.0	37.0	39.0	35.0	39.0
10	37.060250547736864	39.0	37.0	39.0	33.0	39.0
11	36.62405250151782	39.0	35.0	39.0	32.0	39.0
12	35.59836166268343	37.0	35.0	39.0	31.0	39.0
13	35.14618939613914	37.0	35.0	39.0	30.0	39.0
14	36.05723266481346	38.0	35.0	40.0	30.0	41.0
15	36.38874697704074	38.0	35.0	40.0	31.0	41.0
16	36.60293747372995	38.0	35.0	40.0	32.0	41.0
17	36.56630205023138	38.0	35.0	40.0	32.0	41.0
18	36.52202470755217	37.0	35.0	40.0	31.0	41.0
19	36.441587086608415	37.0	35.0	40.0	31.0	41.0
20	36.28703137786026	36.0	35.0	40.0	31.0	41.0
21	36.092544687003155	36.0	35.0	40.0	31.0	41.0
22	36.00230618033995	36.0	34.0	40.0	31.0	41.0
23	35.99177074851619	36.0	34.0	40.0	31.0	41.0
24	35.939909864543	36.0	34.0	40.0	31.0	41.0
25	35.84977745334338	36.0	34.0	40.0	31.0	41.0
26	35.74154332857511	36.0	34.0	40.0	30.0	41.0
27	35.694400393111614	36.0	34.0	40.0	30.0	41.0
28	35.70451905758801	36.0	34.0	40.0	30.0	41.0
29	35.73355215734211	36.0	34.0	40.0	30.0	41.0
30	35.66834304267576	36.0	34.0	40.0	30.0	41.0
31	35.47438987493934	36.0	34.0	40.0	30.0	41.0
32	35.28490896095484	35.0	34.0	40.0	29.0	41.0
33	35.100909476710775	35.0	34.0	40.0	29.0	41.0
34	34.9194842034515	35.0	34.0	40.0	27.0	41.0
35	34.76523358261537	35.0	34.0	40.0	27.0	41.0
36	34.5772045012904	35.0	33.0	40.0	25.0	41.0
37	34.49566277683809	35.0	33.0	40.0	25.0	41.0
38	34.559385032112914	35.0	33.0	40.0	27.0	41.0
39	34.52063683675106	35.0	34.0	40.0	26.0	41.0
40	34.32070678868829	35.0	33.0	40.0	24.0	41.0
41	34.352184652808944	35.0	34.0	40.0	25.0	41.0
42	34.28408862473882	35.0	33.0	40.0	25.0	41.0
43	34.20417234370463	35.0	33.0	40.0	24.0	41.0
44	34.15361912439262	35.0	33.0	40.0	24.0	41.0
45	34.133275699976245	35.0	33.0	40.0	24.0	41.0
46	34.1249997461831	35.0	33.0	40.0	24.0	41.0
47	34.077662386975334	35.0	33.0	40.0	24.0	41.0
48	34.080111212412255	35.0	33.0	39.0	24.0	41.0
49	34.03501150298184	35.0	33.0	39.0	24.0	41.0
50	33.90595880856317	35.0	33.0	39.0	24.0	41.0
51	33.74933703026107	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	7.0
14	21.0
15	40.0
16	144.0
17	413.0
18	955.0
19	1851.0
20	3328.0
21	5454.0
22	8203.0
23	12180.0
24	17478.0
25	24477.0
26	32400.0
27	37900.0
28	41293.0
29	46570.0
30	55781.0
31	68763.0
32	86907.0
33	114409.0
34	206627.0
35	335166.0
36	124414.0
37	148964.0
38	214792.0
39	381152.0
40	229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.58932831926511	23.475321890590703	28.75146452350446	15.183885266639729
2	30.3836087077471	25.496364326745603	30.231826202432178	13.888200763075123
3	28.729737796991152	24.299160779806734	32.7864932860354	14.184608137166713
4	25.592357877765842	27.738633571650478	31.704776427923107	14.96423212266057
5	23.24556683405045	32.90263989879813	29.61906144602533	14.232731821126093
6	21.231529744294704	41.11996198838128	27.30475896532049	10.343749302003529
7	87.5731754118433	4.264174658514745	6.565532477395067	1.597117452246889
8	89.27456084600219	2.9941764250803584	6.256281968238368	1.4749807606790921
9	85.68340707560291	4.393367459861396	7.541306162065135	2.381919302470552
10	54.00756577411108	22.686662023509534	13.608139197248217	9.697633005131163
11	47.6697070546884	19.50542254421998	20.459621792515854	12.365248608575763
12	44.572125625151024	20.639425683427383	22.991191538353764	11.797257153067834
13	21.360367201983426	43.54614695795371	22.04237320830651	13.05111263175635
14	13.957086669333435	45.6867879166912	25.59987085796203	14.75625455601333
15	13.051620265553392	24.049861822080445	49.53774866441548	13.360769247950682
16	14.774377082567652	17.584587019600757	49.354695917202896	18.286339980628693
17	14.433247170956848	18.33080870124939	29.735055768648945	37.50088835914482
18	20.168798390191704	23.040178199768114	36.58496469914576	20.206058710894432
19	28.122963119389375	25.03010268416446	26.1861878935431	20.660746302903057
20	32.1908357885888	21.9391204939886	26.84809160150341	19.021952115919195
21	21.43960883770135	28.116617696926376	29.83480580976728	20.60896765560499
22	22.098669796398237	24.98735991845371	25.808153004887497	27.10581728026056
23	18.11181548120638	30.331830060449033	25.747084659103596	25.809269799240987
24	18.30588388181473	23.107795021533825	42.05923680304418	16.527084293607267
25	16.363676974340127	25.56367656823309	37.813590778121394	20.259055679305394
26	15.516689983979076	34.24106716807349	28.592676671790386	21.64956617615705
27	16.412866689273294	36.82441556120947	28.502470146056396	18.260247603460844
28	14.028510744576947	31.095209764437616	37.75577128863855	17.120508202346894
29	14.566094935642187	23.49029708760338	38.84246295796183	23.101145018792604
30	17.571337777498012	32.108345296569816	32.496888204824145	17.823428721108023
31	27.457455211470087	29.39433196407577	25.016142754745868	18.132070069708274
32	30.319900666218597	25.762059856116277	27.391970451651943	16.526069026013186
33	29.13087002341207	28.582067125432246	24.471857797559704	17.815205053595975
34	19.221807541813796	28.416020110420504	28.049203928679482	24.312968419086218
35	19.236072051510618	26.163445899435718	31.883666577999964	22.716815471053707
36	30.968605895455863	25.918208012085746	26.15902948540147	16.954156607056923
37	19.28536329320319	34.85114146535602	29.336969345010267	16.52652589643052
38	20.265401101768393	33.722620771156656	25.098024086208405	20.91395404086655
39	19.31490758019091	34.101214056989	27.793508785110493	18.790369577709598
40	23.57187383878769	26.3872616405506	26.830070601708496	23.210793918953218
41	17.025631445680137	24.815576641535408	29.03518105267005	29.1236108601144
42	20.920604043607774	25.338997849663237	25.998515678777455	27.741882427951538
43	20.662015387395655	26.741183923846805	26.981700816884306	25.61509987187323
44	18.47451982919138	30.462901106844733	29.469614056176784	21.5929650077871
45	16.188695604500477	40.235816204076905	21.93643003486429	21.639058156558324
46	21.260414107346275	34.80474373630658	25.30275279655459	18.632089359792563
47	20.51038517221984	30.470921720837964	26.798140435874686	22.220552671067516
48	22.243243901795196	25.246100864804937	31.57802026880225	20.932634964597618
49	20.362409920382714	24.99400992119493	32.81136734209035	21.832212816332
50	19.203532725120358	34.97967434276652	26.770474393936013	19.046318538177108
51	17.610527106629494	33.79231889149023	25.0279706222169	23.56918337966338
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1831.0
1	2614.0
2	3397.0
3	9154.5
4	14912.0
5	11116.0
6	7320.0
7	7711.0
8	8102.0
9	8841.0
10	9580.0
11	9932.0
12	10284.0
13	10403.5
14	10523.0
15	9947.0
16	9371.0
17	8769.0
18	8167.0
19	8056.0
20	7945.0
21	9141.5
22	10338.0
23	10469.0
24	10600.0
25	12639.0
26	16295.0
27	17912.0
28	22103.0
29	26294.0
30	30205.5
31	34117.0
32	40129.5
33	46142.0
34	53173.5
35	60205.0
36	63184.5
37	66164.0
38	76947.5
39	87731.0
40	116684.5
41	145638.0
42	177035.0
43	208432.0
44	209005.5
45	209579.0
46	198347.0
47	187115.0
48	171148.5
49	155182.0
50	145888.0
51	136594.0
52	118834.0
53	101074.0
54	89249.0
55	77424.0
56	73843.5
57	70263.0
58	64239.0
59	58215.0
60	54353.5
61	50492.0
62	44668.5
63	38845.0
64	32648.0
65	26451.0
66	19725.0
67	12999.0
68	11312.5
69	9626.0
70	8377.5
71	7129.0
72	5536.5
73	3944.0
74	3397.0
75	2011.5
76	1173.0
77	895.0
78	617.0
79	526.0
80	435.0
81	315.5
82	196.0
83	109.0
84	22.0
85	15.0
86	8.0
87	7.0
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1969924.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.401848370139758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.51375583044418	11.595469031805129
2	7.918072990085526	2.2806977317382175
3	2.7674365753303727	1.1956860579566064
4	1.3716209818423557	0.790155096071833
5	0.7738231612287129	0.5572241916659068
6	0.5214403458543921	0.45058228770409137
7	0.344767396634096	0.3475701438504458
8	0.2664178981615024	0.3069528137930635
9	0.21260155100463043	0.27556697707426997
>10	2.311340954942188	7.981327676390817
>50	1.0092741196730224	10.60078286323058
>100	1.9653164650012094	60.43072279604408
>500	0.02129270276274332	1.941530443116914
>1k	0.0021292702762743324	0.474956365375526
>5k	7.097567587581107E-4	0.7707755241825361
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8412	0.4270215500699519	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6669	0.3385409792458998	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3733	0.18949969643498937	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014213746317116803	0.0
2	0.0	0.0	0.0	0.08370881313187717	0.0
3	0.0	0.0	0.0	0.1293450914857629	0.0
4	0.0	0.0	0.0	0.19843405126289135	0.0
5	0.0	0.0	0.0	0.3343783821101728	0.0
6	0.0	0.0	0.0	0.46057614405428837	0.0
7	0.0	0.0	0.0	0.5357059460161915	0.0
8	0.0	0.0	0.0	0.702209831445274	0.0
9	0.0	0.0	0.0	0.7590140533340373	0.0
10	0.0	0.0	0.0	0.871201122479852	0.0
11	0.0	0.0	0.0	1.0392279093000543	0.0
12	0.0	0.0	0.0	1.168674527545225	0.0
13	0.0	0.0	0.0	1.2193871438695096	0.0
14	0.0	0.0	0.0	1.2372050901456098	0.0
15	0.0	0.0	0.0	1.2650741856030994	0.0
16	0.0	0.0	0.0	1.337818108718915	0.0
17	0.0	0.0	0.0	1.427009366858823	0.0
18	0.0	0.0	0.0	1.5482830809716517	0.0
19	0.0	0.0	0.0	1.605138066240119	0.0
20	0.0	0.0	0.0	1.6630083191026659	0.0
21	0.0	0.0	0.0	1.7458033913998712	0.0
22	0.0	0.0	0.0	1.8324564805545798	0.0
23	0.0	0.0	0.0	1.9328156822293652	0.0
24	0.0	0.0	0.0	2.0113973940111394	0.0
25	0.0	0.0	0.0	2.0694707003925026	0.0
26	0.0	0.0	0.0	2.132772634883376	0.0
27	5.076337970398858E-5	0.0	0.0	2.1951608285395783	0.0
28	5.076337970398858E-5	0.0	0.0	2.2548585630714686	0.0
29	5.076337970398858E-5	0.0	0.0	2.3237444693297813	0.0
30	5.076337970398858E-5	0.0	0.0	2.4165399274286723	0.0
31	5.076337970398858E-5	0.0	0.0	2.501314771534333	0.0
32	5.076337970398858E-5	0.0	0.0	2.578373581924988	0.0
33	5.076337970398858E-5	0.0	0.0	2.6545694148606747	0.0
34	5.076337970398858E-5	0.0	0.0	2.739242732206928	0.0
35	5.076337970398858E-5	0.0	0.0	2.854780184413206	0.0
36	5.076337970398858E-5	0.0	0.0	2.951992056546344	0.0
37	5.076337970398858E-5	0.0	0.0	3.0479348441868823	0.0
38	1.0152675940797717E-4	0.0	0.0	3.1371261023267905	0.0
39	1.0152675940797717E-4	0.0	0.0	3.236368509648088	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATA	45	3.8562575E-10	45.000004	32
ACCGAAA	45	3.8562575E-10	45.000004	14
CTTGCGA	45	3.8562575E-10	45.000004	13
GCGATAC	115	0.0	45.000004	9
ACGTCCA	40	6.8193913E-9	45.000004	26
CGCAACA	20	7.034513E-4	45.000004	36
GCTCGAT	20	7.034513E-4	45.000004	31
TATCGTG	20	7.034513E-4	45.000004	1
CTTACGT	80	0.0	45.000004	22
CGGACGT	20	7.034513E-4	45.000004	23
CGTATGT	25	3.891824E-5	45.0	31
CACGGAT	25	3.891824E-5	45.0	39
TGTGCGA	35	1.2125201E-7	45.0	35
TACGACT	25	3.891824E-5	45.0	15
ACGGATT	25	3.891824E-5	45.0	45
ACCAGTA	55	1.8189894E-12	45.0	22
AACGAGT	25	3.891824E-5	45.0	29
CGTGTCA	25	3.891824E-5	45.0	31
ATCGGCA	25	3.891824E-5	45.0	11
CGAAGTA	35	1.2125201E-7	45.0	18
>>END_MODULE