FastQCFastQC Report
Sat 18 Jun 2016
SRR3549062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699436
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61340.36094327765211515No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC42060.24749387443834306No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18090.10644708009010048No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG207.03424E-445.0000041
ACGTTAT302.166118E-645.00000411
TAGCGAA207.03424E-445.00000433
CGCGATA207.03424E-445.00000437
TCTAGCG207.03424E-445.0000041
CGCAATA207.03424E-445.00000413
ACGTTCA253.8915976E-545.021
CCGTATG253.8915976E-545.038
CTACGTT351.2123928E-745.023
TATAGCG1300.043.2692341
TACGGGA3450.042.3913044
TACGGGC1450.041.8965534
AGGGTAC9250.041.8378376
GTATGCG700.041.7857131
AACGGGA3050.041.3114784
TATTTCG603.6379788E-1241.25000417
ATACCGG603.6379788E-1241.2500042
TATGCCA7750.041.22580710
ACGGGTA2300.041.0869565
CAGGGTA5700.040.6578945